Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,566 G→T 6.9% intergenic (+24/+17) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5660GT 6.9% 405.9 / 13.8 138intergenic (+24/+17)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base G (52/76);  new base T (3/7);  total (55/83)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 141 total aligned reads displayed.

GCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCG  >  NC_000913/654417‑654715
                                                                                                                                                     |                                                                                                                                                     
gCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                                                                       <  1:552013/150‑1 (MQ=255)
  aCTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGcc                                                                                                                                                     <  1:1868393/150‑1 (MQ=255)
     gACCCTCAGCCGCCCCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAACGTTGGCCGACCACAACGCTTATACCACAGTGGGGGACTTGATAACGCCGGAGGAAACTTGCCAcc                                                                                                                                                  >  2:316004/1‑150 (MQ=255)
     gACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTcc                                                                                                                                                  >  2:403660/1‑150 (MQ=255)
       ccATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAACCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                                <  1:1589006/150‑1 (MQ=255)
        cATCAGCCGCACGACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGAAAAGTTGGCTGACCACTACGCATATCCCACAGTGGGTGACTTGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                               <  2:1012124/150‑1 (MQ=255)
        cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCAGAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                                                                               >  2:279721/1‑150 (MQ=255)
        cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                               <  2:458532/150‑1 (MQ=255)
        cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                               <  1:1284945/150‑1 (MQ=255)
        cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCAGCTGATTATTGCTGAAATTGGTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                               <  1:11186/150‑1 (MQ=255)
         aTCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGct                                                                                                                                              <  2:1204069/150‑2 (MQ=255)
         aTCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                              >  1:1732240/1‑150 (MQ=255)
         aTCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                              >  2:1590634/1‑150 (MQ=255)
          tCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAt                                                                                                                                             <  2:1135038/150‑1 (MQ=255)
             gCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                                                                          >  1:1684759/1‑150 (MQ=255)
             gCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCAATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAGGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTaa                                                                                                                                          >  2:1485685/1‑149 (MQ=255)
                   ccacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTa                                                                                                                                    >  2:859407/1‑150 (MQ=255)
                       tGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTg                                                                                                                                <  2:869088/150‑1 (MQ=255)
                         ccAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCTGGCATTACTATTTACTTGcc                                                                                                                              <  1:1281278/150‑1 (MQ=255)
                         ccAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGcc                                                                                                                              <  1:1007048/150‑1 (MQ=255)
                           aGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtg                                                                                                                            <  1:1042629/150‑1 (MQ=255)
                              cGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGa                                                                                                                         >  2:71224/1‑150 (MQ=255)
                                    cTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGCCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACCTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                                   >  1:1335358/1‑150 (MQ=255)
                                    cTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                                   >  1:1491049/1‑150 (MQ=255)
                                    cTTGAATATTGCGCCAGCCGCCATCGGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                                   >  1:708009/1‑150 (MQ=255)
                                        aaTATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGACGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCtgctgc                                                                                                               <  1:1732991/150‑1 (MQ=255)
                                           atTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGt                                                                                                            >  1:1321965/1‑150 (MQ=255)
                                             tGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTc                                                                                                          <  2:1597076/150‑1 (MQ=255)
                                             tGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTc                                                                                                          <  1:195000/150‑1 (MQ=255)
                                              gcgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                                         >  1:1375099/1‑150 (MQ=255)
                                                gcCATCCGCCATCTGATTATTGCTTAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                                                                       <  2:1182891/150‑1 (MQ=255)
                                                gcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                                                                       <  2:530491/150‑1 (MQ=255)
                                                 ccAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                                      >  2:11713/1‑150 (MQ=255)
                                                 ccAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                                      <  1:1187388/150‑1 (MQ=255)
                                                  cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                                                                     <  1:1624810/150‑1 (MQ=255)
                                                  cAGCCGCCATCTGATTATTGCTGAAAATGTTCACGGCAAAGTTTGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                                                                     <  1:1472540/150‑1 (MQ=255)
                                                   aGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCa                                                                                                    <  1:737611/150‑1 (MQ=255)
                                                   aGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCa                                                                                                    <  1:925746/150‑1 (MQ=255)
                                                   aGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCa                                                                                                    >  1:475598/1‑150 (MQ=255)
                                                     ccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAGCGCTGCTGCCGTTCCTGGCAcc                                                                                                  <  1:1040876/150‑1 (MQ=255)
                                                     ccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGGGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                                                                                  <  2:234734/150‑1 (MQ=255)
                                                             tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                                                                          >  2:1059409/1‑150 (MQ=255)
                                                                     gCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                                  >  2:1012273/1‑150 (MQ=255)
                                                                      cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                                 >  1:1834753/1‑150 (MQ=255)
                                                                       tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACg                                                                                >  1:748426/1‑150 (MQ=255)
                                                                       tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGAATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTATCAACg                                                                                >  2:791313/1‑150 (MQ=255)
                                                                          atttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATa                                                                             <  2:141375/148‑1 (MQ=255)
                                                                           aTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATaa                                                                            <  2:804324/150‑1 (MQ=255)
                                                                            ttGTTCACGGCAAAGTTGGCAGACCACATCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                                                                           <  2:572077/150‑1 (MQ=255)
                                                                               ttCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAAt                                                                        >  2:1752873/1‑150 (MQ=255)
                                                                                tCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATc                                                                       >  2:165764/1‑150 (MQ=255)
                                                                                   cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                                                                    <  1:1177112/150‑1 (MQ=255)
                                                                                   cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                                                                    <  2:1865592/150‑1 (MQ=255)
                                                                                   cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                                                                    >  1:495771/1‑150 (MQ=255)
                                                                                      cAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaa                                                                 >  2:1186207/1‑150 (MQ=255)
                                                                                           ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaac                                                            <  2:546639/150‑1 (MQ=255)
                                                                                               cAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc                                                        >  1:1233332/1‑150 (MQ=255)
                                                                                               cAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCACCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc                                                        <  2:1732240/150‑1 (MQ=255)
                                                                                                   ccACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCg                                                    <  1:859407/150‑1 (MQ=255)
                                                                                                     acaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGt                                                  <  1:1812723/150‑1 (MQ=255)
                                                                                                       aaCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTAc                                                >  1:851393/1‑150 (MQ=255)
                                                                                                         cGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCg                                              >  1:567770/1‑150 (MQ=255)
                                                                                                           cATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAg                                            <  2:1461939/150‑1 (MQ=255)
                                                                                                              ataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTg                                         <  2:1245138/150‑1 (MQ=255)
                                                                                                                 ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCTGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTCGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGt                                      >  2:1083497/1‑150 (MQ=255)
                                                                                                                 ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTACCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCCCCGGTACCGAGGTGCGt                                      >  2:1697077/1‑150 (MQ=255)
                                                                                                                    caGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGtttt                                   <  1:857953/150‑1 (MQ=255)
                                                                                                                    caGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGtttt                                   >  2:1761636/1‑150 (MQ=255)
                                                                                                                     aGTTGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTa                                  <  1:1533298/150‑1 (MQ=255)
                                                                                                                      gTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                                 <  2:913900/150‑1 (MQ=255)
                                                                                                                      gTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                                 <  1:161215/150‑1 (MQ=255)
                                                                                                                      gTGGGAGACATGATTAAGCCGGAGGCAACTTGCCTCCGTCATTACTCTTTACTAGCCTGTGACCGCTGCTGCCGTTCCTTGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGCTACCGAGGTGCGTTTTAc                                 <  2:215179/150‑1 (MQ=255)
                                                                                                                       tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACg                                <  2:814075/150‑1 (MQ=255)
                                                                                                                          ttGACATGATAAAGCCTGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                             <  1:1485685/149‑1 (MQ=255)
                                                                                                                          gTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGCCCGCGGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                             <  2:601465/150‑1 (MQ=255)
                                                                                                                          gTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                             <  1:1223415/150‑1 (MQ=255)
                                                                                                                            gACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTc                           <  1:11713/150‑1 (MQ=255)
                                                                                                                             aaatagtAATGCCGGAGGCAAGTTTCCTCCGGCTTTACTATTTACTTGCCTGTGACCGCTGCTGCAGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCa                          >  2:167715/7‑150 (MQ=38)
                                                                                                                             aCATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCa                          <  2:856846/150‑1 (MQ=255)
                                                                                                                                tGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAAc                       <  2:1474576/150‑1 (MQ=255)
                                                                                                                                 gATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCCAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACg                      >  2:728270/1‑150 (MQ=255)
                                                                                                                                   tAAAGCCGGAGGAAACCTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGc                    <  2:1335358/150‑1 (MQ=255)
                                                                                                                                    aatgCCGGAGGCAAGTTTCCTCCGGCTTTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCCCCAAACCAAGAAGCACCGGTACCGAGGTGCGGTTTACGACTTCAAACGGCg                   >  1:464086/4‑150 (MQ=255)
                                                                                                                                    aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCg                   <  1:69779/150‑1 (MQ=255)
                                                                                                                                     aaGCCGGAGGAAACTTTCCTCCGGCATTACTATTTACTTGCCTTTGACCGCTGCTGCCTTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATATCCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCga                  <  2:1489855/150‑1 (MQ=255)
                                                                                                                                       gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgaga                <  2:1834753/150‑1 (MQ=255)
                                                                                                                                       gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgaga                >  1:231087/1‑150 (MQ=255)
                                                                                                                                         cGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATc              <  1:1692861/150‑1 (MQ=255)
                                                                                                                                         cGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGAGAACAATCACCAAACCAACAAGGACCGGTACCGAGGTGCGTTTGACGACTGCAAACGGCGAGATc              >  1:79052/1‑150 (MQ=255)
                                                                                                                                          ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCt             >  1:805242/1‑150 (MQ=255)
                                                                                                                                          ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCt             <  2:1321965/150‑1 (MQ=255)
                                                                                                                                           gaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCtt            <  1:1688369/150‑1 (MQ=255)
                                                                                                                                             ggaAACTTGCCTCCTGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCTTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATAAGCAAACCAACTAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCg          <  1:1119762/150‑1 (MQ=255)
                                                                                                                                             ggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCg          <  2:475598/150‑1 (MQ=255)
                                                                                                                                              gaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGc         <  2:567770/150‑1 (MQ=255)
                                                                                                                                                aaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCAGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCa       >  2:1801909/1‑150 (MQ=255)
                                                                                                                                                  cTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACAATCAGCTCTGTAGCAACGATAACAATCACCAATCCAACAAGCACCTGTACCGAGGTGCGGTTTACGACTTCAAACGTCGAGATCTTCGCCATc     >  1:937031/1‑150 (MQ=255)
                                                                                                                                                    tGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATccc   >  2:258886/1‑150 (MQ=255)
                                                                                                                                                    tGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATccc   <  2:213374/150‑1 (MQ=255)
                                                                                                                                                     gCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCg  <  1:71224/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCG  >  NC_000913/654417‑654715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: