Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 10,619 G→T 5.2% intergenic (‑125/+24) satP ← / ← yaaW succinate‑acetate transporter/UPF0174 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,6190GT 5.2% 338.9 / 7.4 116intergenic (‑125/+24)satP/yaaWsuccinate‑acetate transporter/UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base G (52/58);  new base T (2/4);  total (54/62)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

Only 100 of 128 total aligned reads displayed.

GATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10470‑10768
                                                                                                                                                     |                                                                                                                                                     
gATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatg                                                                                                                                                       <  2:689065/150‑1 (MQ=255)
  ttAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaa                                                                                                                                                     <  2:1524941/150‑1 (MQ=255)
   tAGCCAACTTAGTGTTGCCCATAATTCATCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAATGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTATGAGGGgaaaatgaaa                                                                                                                                                    >  1:1785966/1‑150 (MQ=255)
    aGCCAACTTAGTGCTGCGCATAAAACCTCTCCACGCCTCATCGACTGAATCGTGACAAACTTTCCCTGACTACCTGTAGCGTATTCAGGGCGCGGCCTACTCATCAGCCAGTGGCGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaaa                                                                                                                                                   <  2:1821538/150‑1 (MQ=255)
     gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTTAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                  >  1:439538/1‑150 (MQ=255)
     gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCTGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGCGGGgaaaatgaaaat                                                                                                                                                  >  2:1727304/1‑150 (MQ=255)
     gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                  >  2:808144/1‑150 (MQ=255)
       cAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAAttt                                                                                                                                                >  1:1871892/1‑150 (MQ=255)
       cAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAAttt                                                                                                                                                >  1:1578572/1‑150 (MQ=255)
          cTTATTGTTGCCCATAATTCCTCAAAAATCATAATCGAATGAATCGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcc                                                                                                                                             <  1:107241/150‑1 (MQ=255)
          cTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcc                                                                                                                                             <  1:1021473/150‑1 (MQ=255)
            tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGGTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                           <  1:999403/150‑1 (MQ=255)
              gtgtTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCgg                                                                                                                                         >  1:1288665/1‑150 (MQ=255)
              gtgtTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACCGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccctc                                                                                                                                         <  2:1714121/150‑3 (MQ=255)
                gtTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATTATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGtt                                                                                                                                       >  2:1622208/1‑150 (MQ=255)
                   gCCCATAATTCCTCAAAAATCATAATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCTGCCAGTGGGGCAGTGTCTACGCTCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTcc                                                                                                                                    >  2:75370/1‑150 (MQ=255)
                    cccATAATTCCTCAAAAATCATCATCCAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCTGCATAATAATCAGCCAGGGGGCCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCg                                                                                                                                   <  2:898330/150‑1 (MQ=255)
                     ccATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCgg                                                                                                                                  <  2:1288665/150‑1 (MQ=255)
                       aTAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTa                                                                                                                                <  1:20132/150‑1 (MQ=255)
                         aaTTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGAATc                                                                                                                              <  1:1394424/150‑1 (MQ=255)
                             ccTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGAc                                                                                                                          <  2:550227/150‑1 (MQ=255)
                             ccTCAAAAATCATCATCGAATCAATGGAGAAAAAAGTTCCCTGAATAACGGCCCTGTTTTAAGGGCGAGGCAAAATAATCAGCCAGTGGGGCAGTGGCAACGAGCTTTTGAGGCGAAACTGAAAATTGTCCCCGGTTTCCGGTATCAGAc                                                                                                                          <  2:319603/150‑1 (MQ=255)
                              cTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAATATGAAAATTTTCCCCGGTTTCCGGTATCAGAcc                                                                                                                         <  1:15047/150‑1 (MQ=255)
                              cTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGAcc                                                                                                                         >  1:978266/1‑150 (MQ=255)
                                  aaaaTCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAg                                                                                                                     <  2:1369224/150‑1 (MQ=255)
                                      tcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgc                                                                                                                 <  1:905937/150‑1 (MQ=255)
                                      tcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgc                                                                                                                 <  2:649571/150‑1 (MQ=255)
                                            tcGAATGAATGGTGAAATAATTTCACTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAAc                                                                                                           <  2:1362527/150‑1 (MQ=255)
                                                 tgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATcc                                                                                                      >  1:52161/1‑150 (MQ=255)
                                                  gaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCg                                                                                                     >  2:1810395/1‑150 (MQ=255)
                                                     tgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                  >  1:916233/1‑150 (MQ=255)
                                                     tgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                  >  1:1273884/1‑150 (MQ=255)
                                                     tgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                  <  1:996267/150‑1 (MQ=255)
                                                        tGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgcag                                                                                               >  2:1032175/1‑150 (MQ=255)
                                                         gAAATAATTTCCCTGAATACCTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgcagg                                                                                              <  1:942229/150‑1 (MQ=255)
                                                         gAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGTGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgcagg                                                                                              >  1:225264/1‑150 (MQ=255)
                                                         gAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGAATCAGACCTGAGTGGCGCTAACCATCCGGCgcagg                                                                                              <  1:1469951/150‑1 (MQ=255)
                                                          aaaTAATTTCCCTGAATAACTGTAGTGTTATCAGGGCGCTGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCtcaggc                                                                                             >  1:1162774/1‑150 (MQ=255)
                                                             taatTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGcaggcagg                                                                                          <  1:1688410/150‑1 (MQ=255)
                                                               atTTCCCTGAATAACTGTAGTGTTATCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCg                                                                                        <  2:1362319/150‑1 (MQ=255)
                                                                  tCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCTAtt                                                                                     <  2:1780747/150‑1 (MQ=255)
                                                                   cccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGAttt                                                                                    >  2:960663/1‑150 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   >  2:1621072/1‑150 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  2:1610434/150‑1 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  2:1016168/150‑1 (MQ=255)
                                                                    ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   >  2:858782/1‑150 (MQ=255)
                                                                    ccTGAATAACTGTAGTGAGTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  1:1318979/150‑1 (MQ=255)
                                                                     cTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCGACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGAATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGc                                                                                  <  1:959282/150‑1 (MQ=255)
                                                                      tGACTAACTGTCGTGTTATCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCa                                                                                 <  1:588281/150‑1 (MQ=255)
                                                                      tGAATAACTGTAGTGTTTTCAGGGCGCGGCATACTAATCAGCCAGTGGGGCAGTGTCTACGCTCGTTTGAGGGGAAACTGACAATTTTCCCCGGTGTCCGGTATCAGACCTGAGGGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCa                                                                                 <  1:120357/150‑1 (MQ=255)
                                                                       gAATAACTGTAGTGTTTTCAGGGCGCGGCATAATTATCACGCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAAATTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                <  2:1163478/150‑1 (MQ=255)
                                                                       gAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTATACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                <  2:1622568/150‑1 (MQ=255)
                                                                        aaTAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                               <  2:18774/150‑1 (MQ=255)
                                                                            aCTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACgg                                                                           <  1:1285345/150‑1 (MQ=255)
                                                                             cTGTAGCGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGc                                                                          <  2:444708/150‑1 (MQ=255)
                                                                               gTAGTGTTTTCAGGGCGCGGCATAATTATCAGCCAGTGGGGGAGTGTCTACGAGCTTTTGAGGGGAAAATGAAAATTTTCGCCGGTTTCCGGTATCTGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                        <  1:976534/150‑1 (MQ=255)
                                                                               gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGTGCAGTCAGGCGATTTGCAGTACGGCTg                                                                        >  2:1019304/1‑150 (MQ=255)
                                                                                 aGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGa                                                                      >  2:1792956/1‑150 (MQ=255)
                                                                                  gtgtTTTCAGGGCGCCGCAGAATAATCAGCCCGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGaa                                                                     <  1:1213160/150‑1 (MQ=255)
                                                                                   tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                                                    >  1:123313/1‑150 (MQ=255)
                                                                                   tgtTTTCAGGGAGCGGCATAATAATCAGCCAGTGGGGCATTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                                                    <  1:1650517/150‑1 (MQ=255)
                                                                                    gtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                                                   <  2:332782/150‑1 (MQ=255)
                                                                                         cAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGCTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGTCAGGCGATTTGCAGTACGGCTGGAATCGTCAc                                                              >  2:1068728/1‑150 (MQ=255)
                                                                                          aGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcg                                                             >  2:1250612/1‑150 (MQ=255)
                                                                                          aGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcg                                                             >  1:1410344/1‑150 (MQ=255)
                                                                                               gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATa                                                        >  2:574766/1‑150 (MQ=255)
                                                                                                  gCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCCGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                     >  2:1155956/1‑150 (MQ=255)
                                                                                                      aatcatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTg                                                 <  1:1244987/150‑1 (MQ=255)
                                                                                                       ataatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGCATCGTCACGCGATAGGCGCTGc                                                >  2:54052/1‑150 (MQ=255)
                                                                                                         aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCg                                              >  2:1364936/1‑150 (MQ=255)
                                                                                                             aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGa                                          >  1:230148/1‑150 (MQ=255)
                                                                                                              gCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAc                                         <  1:1453564/150‑1 (MQ=255)
                                                                                                               ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                        >  1:598761/1‑150 (MQ=255)
                                                                                                                cAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGAGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCg                                       >  1:38649/1‑150 (MQ=255)
                                                                                                                    ggggCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCttt                                   <  1:472011/150‑1 (MQ=255)
                                                                                                                       gCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCAGCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAc                                >  2:1531102/1‑150 (MQ=255)
                                                                                                                       gCAGTGTCTACGATCTTTGGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAc                                <  2:326689/150‑1 (MQ=255)
                                                                                                                            gtCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAt                           >  1:1174087/1‑150 (MQ=255)
                                                                                                                              cTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAttt                         >  1:1227880/1‑150 (MQ=255)
                                                                                                                              cTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAttt                         >  1:1088011/1‑150 (MQ=255)
                                                                                                                              cTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAttt                         >  2:960124/1‑150 (MQ=255)
                                                                                                                               tACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTa                        >  1:766481/1‑150 (MQ=255)
                                                                                                                               tACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTa                        >  1:430759/1‑150 (MQ=255)
                                                                                                                               tACGATCATTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCTGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTa                        <  1:1790134/150‑1 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     <  2:439538/150‑1 (MQ=255)
                                                                                                                                  gATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGTATCAGACCTGAGTGGCGATAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                     >  2:1405961/1‑150 (MQ=255)
                                                                                                                                     cTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCAGCTGACCGCTTTAACCCCATTTAGTGCCg                  <  1:1622208/150‑1 (MQ=255)
                                                                                                                                      ttttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGc                 <  1:70495/150‑1 (MQ=255)
                                                                                                                                       tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCa                <  2:1398155/150‑1 (MQ=255)
                                                                                                                                         tGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGAGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCAcc              >  2:1722844/1‑150 (MQ=255)
                                                                                                                                           cggggAAAATTTTCATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTa            >  2:83663/2‑150 (MQ=255)
                                                                                                                                           cggggAAAATTTTCATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGAGAGTTGCAGTACGGCTGGAATCGTCACGCGATAGCCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTa            >  1:1228457/2‑150 (MQ=255)
                                                                                                                                            ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGCCCGCTTTAACCCCATTTAGTGCCGCACCTAc           >  2:1810389/1‑150 (MQ=255)
                                                                                                                                            ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc           <  2:123313/150‑1 (MQ=255)
                                                                                                                                             gggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACa          >  2:1752628/1‑150 (MQ=255)
                                                                                                                                             gggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCGAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCGGGAAGCGGCACGCGATAGGCGCTGCCGCGGACCGCTGTACCCCCATTTAGTGCCGAACCTAcc          >  2:1745437/1‑149 (MQ=255)
                                                                                                                                              ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAg         >  1:1099686/1‑150 (MQ=255)
                                                                                                                                               gaaaatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAgg        <  2:1578572/150‑1 (MQ=255)
                                                                                                                                                aaaatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg       <  1:1810395/150‑1 (MQ=255)
                                                                                                                                                     gaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCTCCTACAGGGCCTcc  >  2:1004924/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10470‑10768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑