Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 662,683 A→T 5.1% intergenic (‑41/+69) ybeD ← / ← dacA UPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,6830AT 5.1% 439.1 / 6.5 147intergenic (‑41/+69)ybeD/dacAUPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base A (80/59);  new base T (6/2);  total (86/61)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAA  >  NC_000913/662539‑662832
                                                                                                                                                |                                                                                                                                                     
aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGATAAGTTTGGTTTTCATGTCAGCTCAGCCGTAACGTAATTTAATAGCAACTCCCGCCAGAAgg                                                                                                                                                  >  2:527983/1‑150 (MQ=255)
   aCTTCAACCACCTGATCAACCCGCTCAGGGAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGATCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                                                                                                                               <  2:194778/150‑1 (MQ=255)
    cTTCAACCACCTGATCAACCATCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                                                              <  1:1651666/150‑1 (MQ=255)
    attCAAACACCTTATCAACCAGCTCATGTAACGCCTGCCGCATATCTTTGTAACTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAgtcttg                                                                                                                                              <  2:349464/149‑6 (MQ=255)
      tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                                                                                                                            <  2:1539442/150‑1 (MQ=255)
      tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggggg                                                                                                                                            <  1:1787816/150‑3 (MQ=255)
       cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAGt                                                                                                                                           <  2:186336/150‑1 (MQ=255)
         accaccTGATCAACCAGCGCAGGTAACGCCTGCCCCATAACTTTGAAAGTAAAACGAGAAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCACGCCAGAAGGCGGGAGttt                                                                                                                                         >  1:1192392/1‑150 (MQ=255)
          ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttt                                                                                                                                        >  1:1748875/1‑150 (MQ=255)
           caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                                                                                                                       >  2:1150862/1‑150 (MQ=255)
           caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                                                                                                                       <  1:586118/150‑1 (MQ=255)
           caccAGATCAACAAGATACGGTAACGCATTAACAATCACTTTTTAATTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTTGTTTTCATGTAATCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCAAGAAGGCGGGAtttttt                                                                                                                                       <  2:441659/150‑1 (MQ=255)
            accTGATACACCATCTCATGAAACGACAGCCCACTAACATTGTAATTAAAAGGAGTAGGGAAATCAAGCAGTTCCGTAACTTTTGTTTTCATGTCAGCTCCGGCGTAACGTAATTTAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                                                                                                                      <  2:1651157/150‑1 (MQ=255)
              cTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                                                                                                                                    <  1:565009/150‑1 (MQ=255)
               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                                                                                                                   >  2:1692005/1‑150 (MQ=255)
               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                                                                                                                   <  2:864/150‑1 (MQ=255)
                  tCAACCAGCTCATGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                                                                                                                                <  1:866672/150‑1 (MQ=255)
                  tCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                                                                                                                                <  2:22650/150‑1 (MQ=255)
                    aaCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTTAGTTTGGTTTTCATGTGAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                                                                                                                              >  2:1014143/1‑150 (MQ=255)
                    aaCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATgg                                                                                                                              >  2:1367627/1‑149 (MQ=255)
                    aaCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                                                                                                                              <  2:518648/150‑1 (MQ=255)
                        aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGATCGTTAAGTTTGGTTTTCTTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                                                                                                                          >  1:1326449/1‑150 (MQ=255)
                         gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                                                                                                                         <  1:1863322/150‑1 (MQ=255)
                           tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTTGGGAATTCAAGCAGTTCGTTAAGGTTGGTTTTGGTGTCAGCTCCGGCGGAACGTAATTTATTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGgat                                                                                                                       >  2:1216175/1‑150 (MQ=255)
                             aGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTatat                                                                                                                     <  1:1192638/150‑1 (MQ=255)
                              ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATg                                                                                                                    >  1:1692040/1‑150 (MQ=255)
                                   cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc                                                                                                               >  2:27699/1‑150 (MQ=255)
                                        gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGaaa                                                                                                          <  1:829618/150‑1 (MQ=255)
                                        gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGaaa                                                                                                          <  1:1016968/150‑1 (MQ=255)
                                        gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAAGGaaa                                                                                                          >  2:595046/1‑150 (MQ=255)
                                           ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                       <  1:105343/150‑1 (MQ=255)
                                                   ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCa                                                                                               <  2:1185343/150‑1 (MQ=255)
                                                   ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCACGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCa                                                                                               <  1:265982/150‑1 (MQ=255)
                                                     gtaagtaaTAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAg                                                                                             <  1:1388208/150‑1 (MQ=255)
                                                      taagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgt                                                                                            >  2:1194531/1‑150 (MQ=255)
                                                      taactaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCACCGGCGTAACGTAAATAAATATCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGAGACGGAAATTACACTTACAAgt                                                                                            >  1:936513/1‑150 (MQ=255)
                                                       aagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtg                                                                                           >  2:1262463/1‑150 (MQ=255)
                                                       aagtaaAAGGAGAAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATCTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGCGTTTTTTACTGATGCTTAGTATATGGGGCCGGTAATTACACTTTCAAgtg                                                                                           >  2:705025/1‑150 (MQ=255)
                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtgt                                                                                          >  1:658593/1‑150 (MQ=255)
                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtgt                                                                                          >  2:816428/1‑150 (MQ=255)
                                                          taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                                                                        >  1:705658/1‑150 (MQ=255)
                                                          taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                                                                        >  2:1595650/1‑150 (MQ=255)
                                                           aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTa                                                                                       >  2:510361/1‑150 (MQ=255)
                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtt                                                                                    >  2:1166581/1‑150 (MQ=255)
                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTGACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttt                                                                                   >  2:1755147/1‑150 (MQ=255)
                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttt                                                                                   >  1:1595385/1‑150 (MQ=255)
                                                                taGTAGGGAATTAAAGCAGTTCGTTTAGTTTGGTTTTCATGTCAGCTCCTGAGTATCGTAATTAAATTGCAACTCCCGCCAGAAGGCGTGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtttt                                                                                  <  1:1732496/149‑1 (MQ=255)
                                                                  gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTGAAGGCGGGAGTTTTTTACTGATGATTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTa                                                                                >  1:361380/1‑150 (MQ=255)
                                                                  gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTa                                                                                <  2:621153/150‑1 (MQ=255)
                                                                     gggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacc                                                                             <  1:1595650/150‑1 (MQ=255)
                                                                     gggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTAAAGTGTTTAATTTTTaacc                                                                             >  2:1315631/1‑150 (MQ=255)
                                                                      ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacca                                                                            >  1:1092233/1‑150 (MQ=255)
                                                                         aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                                                                         <  1:1031607/150‑1 (MQ=255)
                                                                                cAGTTCGTTAAGTTTGTTTTTCATGTAAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGAt                                                                  <  2:1690323/150‑1 (MQ=255)
                                                                                  gTTCGTTAAGGTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATgg                                                                <  2:967099/150‑1 (MQ=255)
                                                                                     cGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGaaa                                                             >  1:591833/1‑149 (MQ=255)
                                                                                     cGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                             >  2:75483/1‑150 (MQ=255)
                                                                                     cGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                             <  2:1100644/150‑1 (MQ=255)
                                                                                      gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAACCCAGTGATGGAACa                                                            >  2:1018733/1‑150 (MQ=255)
                                                                                      gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                            <  1:510361/150‑1 (MQ=255)
                                                                                       ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGCAGTTATTTACTGATGCTTAGTATATGGGGAAGGAAATTAAAATTTCAAGTGTTAAATTAATAAACAAACCAGTGATGGAACAt                                                           >  2:468528/1‑150 (MQ=255)
                                                                                        tAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAtt                                                          >  1:512224/1‑150 (MQ=255)
                                                                                           gTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaat                                                       >  1:1663580/1‑150 (MQ=255)
                                                                                            tttGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatt                                                      <  1:1792084/150‑1 (MQ=255)
                                                                                               tgtattCATGTCACCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGTCGGGAGTTTTTTACTGATTCTTAGTATATGGGTACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaa                                                   <  2:242628/146‑1 (MQ=255)
                                                                                                 ttttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAAACAAACCAGTGATGGAACATTAATTTAATg                                                 >  1:359943/1‑150 (MQ=255)
                                                                                                  tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGATTTTTACTGATGCTTAGTATATGGGGACGGAAAATACACTTTCAAGTGTTTAATTTTAAACCAAACCATTGATGGAACAttaatttaatct                                                >  2:647517/1‑148 (MQ=255)
                                                                                                      aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATc                                            >  2:134877/1‑150 (MQ=255)
                                                                                                      aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATc                                            >  1:610476/1‑150 (MQ=255)
                                                                                                        gTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCaa                                          >  1:672631/1‑150 (MQ=255)
                                                                                                         tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                         <  2:1580635/150‑1 (MQ=255)
                                                                                                             cTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtt                                     >  1:1510273/1‑150 (MQ=255)
                                                                                                               ccGGCGTAACTTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATTCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtttt                                   <  2:366187/150‑1 (MQ=255)
                                                                                                                cGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTg                                  <  1:1722298/150‑1 (MQ=255)
                                                                                                                 ggCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGc                                 <  2:1608153/150‑1 (MQ=255)
                                                                                                                   cgtaacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCg                               <  2:1538729/150‑1 (MQ=255)
                                                                                                                      aacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                            <  2:872960/150‑1 (MQ=255)
                                                                                                                       acgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaaga                           >  2:1246069/1‑150 (MQ=255)
                                                                                                                         gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                         <  2:689627/150‑1 (MQ=255)
                                                                                                                         gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                         <  2:1326449/150‑1 (MQ=255)
                                                                                                                            aTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTa                      >  2:1032455/1‑150 (MQ=255)
                                                                                                                             ttAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                     >  1:203259/1‑150 (MQ=255)
                                                                                                                              tAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAcc                    >  1:966457/1‑150 (MQ=255)
                                                                                                                                  tAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATTGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc                <  2:532672/150‑1 (MQ=255)
                                                                                                                                  tAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc                >  2:580045/1‑150 (MQ=255)
                                                                                                                                  tAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGAGATTGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc                >  1:422478/1‑150 (MQ=255)
                                                                                                                                  aaaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCACACCAGTGATGGAACATTAATTTAATGTAATCAACTATTTTGCCGAAGAAGTTACCTTcc                >  2:340468/5‑150 (MQ=255)
                                                                                                                                   aaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGTTGCTTAGTATATGGGGACGGATATTACACTTTCAAGTGTTTAATTTTTACCCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg               >  2:1166627/4‑150 (MQ=255)
                                                                                                                                     cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg             >  2:1821999/1‑150 (MQ=255)
                                                                                                                                     cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg             <  2:1692040/150‑1 (MQ=255)
                                                                                                                                      aaCTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGa            <  2:366191/150‑1 (MQ=255)
                                                                                                                                       aCTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt           >  2:1797781/1‑150 (MQ=255)
                                                                                                                                          cccGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAttta        >  2:1626949/1‑149 (MQ=255)
                                                                                                                                            cGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt      >  2:960164/1‑150 (MQ=255)
                                                                                                                                              ccAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGTGATTTCTTGc    >  1:533992/1‑150 (MQ=255)
                                                                                                                                              ccAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGc    <  2:17636/150‑1 (MQ=255)
                                                                                                                                               cAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAGGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCa   <  2:1163703/150‑1 (MQ=255)
                                                                                                                                               cAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCa   <  1:1543969/150‑1 (MQ=255)
                                                                                                                                               cAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCa   <  1:1150862/150‑1 (MQ=255)
                                                                                                                                                aGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCaa  <  1:27699/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAA  >  NC_000913/662539‑662832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: