BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	672,242	T→G	5.8%	I848L (ATT→CTT)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,242	0	T	G	5.8%	394.6 / 7.9	128	I848L (ATT→CTT)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (55/64); new base G (4/4); total (60/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAG  >  NC_000913/672093‑672390

 ggaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATc                                                                                                                                                     >  1:972357/1‑150 (MQ=255)

 ggaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATc                                                                                                                                                     >  1:1797466/1‑150 (MQ=255)

  gaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCa                                                                                                                                                    >  2:1043727/1‑150 (MQ=255)

    aggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt                                                                                                                                                  >  2:1441034/1‑150 (MQ=255)

       catcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGATTACCTGGTAAGTAAATCACATTa                                                                                                                                               >  1:677230/4‑150 (MQ=255)

       aaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCTACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTa                                                                                                                                               >  2:1839923/1‑150 (MQ=255)

       aaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTa                                                                                                                                               <  2:605899/150‑1 (MQ=255)

        atcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTAc                                                                                                                                              >  2:1072713/3‑150 (MQ=255)

            gCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAc                                                                                                                                          <  2:846406/150‑1 (MQ=255)

             ggttgTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAGTGATTTACGTAcc                                                                                                                                         <  2:946177/146‑2 (MQ=255)

             cGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAGTGATTTACGTAcc                                                                                                                                         <  1:1082657/150‑2 (MQ=255)

                ggTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTATACGTACAgga                                                                                                                                      >  1:354227/1‑148 (MQ=255)

                  tATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAAc                                                                                                                                    >  2:106410/1‑150 (MQ=255)

                   aTCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACg                                                                                                                                   <  1:1843788/150‑1 (MQ=255)

                     cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAACTCACTTTACGTACAGTAACGcc                                                                                                                                 >  1:240937/1‑150 (MQ=255)

                     cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGcc                                                                                                                                 >  2:1855268/1‑150 (MQ=255)

                     cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGACAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAACACTTTACGTACAGTAACGcc                                                                                                                                 >  1:350771/1‑150 (MQ=255)

                          aGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCaa                                                                                                                            >  2:771170/1‑150 (MQ=255)

                            aTGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                                                                                                                          <  1:661566/150‑1 (MQ=255)

                             tGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGat                                                                                                                         >  2:972593/1‑150 (MQ=255)

                              gccagccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGata                                                                                                                        >  2:1201616/1‑150 (MQ=255)

                              gccagccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGata                                                                                                                        <  1:1126330/150‑1 (MQ=255)

                                gcgccaCACCCGGCGGTGATTACCACCGCCAGAGATAACAACAATGGTGCCAGATATCGCACGCTTCCTCCCGCGCTTCGCCAACGCCCAGATTGAGGTGTTTACCTGGTACGTAAATCACTGTCCGTCCAGTCACGCCAGCAAGATAtt                                                                                                                      >  1:1477626/3‑150 (MQ=255)

                                  gccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATAtttt                                                                                                                    <  1:157499/150‑1 (MQ=255)

                                   ccaCACCCGGCGGTGATTAACACCGCCAGAGATAAGAACAATGTTGAGAGATATCGCACGCTTCCTCCCGCGCTTACCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTg                                                                                                                   <  2:755028/150‑1 (MQ=255)

                                   ccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTg                                                                                                                   <  2:280871/150‑1 (MQ=255)

                                   ccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTg                                                                                                                   <  2:1763210/150‑1 (MQ=255)

                                        cccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc                                                                                                              <  1:459415/150‑1 (MQ=255)

                                         ccGGCGGTTATTAACACCGCCAGAGATAACACCAATGTTGCCAGATATCGCACGCTTCCTCCCTCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGAAATTTTGCTACCa                                                                                                             <  2:1498175/150‑1 (MQ=255)

                                          ggggggTGTTTAACACCTCAAGAGATCACAACAATGTTGCGAGATATAGCACGCTTCCTCCCGCGGTTAGCCACCGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCCTCAAGTTATTTTGCTACCAg                                                                                                            <  2:330393/146‑1 (MQ=255)

                                           ggcggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGa                                                                                                           <  1:810201/150‑1 (MQ=255)

                                              ggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGt                                                                                                        <  2:1744369/150‑1 (MQ=255)

                                               gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGtt                                                                                                       <  1:1750861/150‑1 (MQ=255)

                                               gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGtt                                                                                                       >  1:827453/1‑150 (MQ=255)

                                                 gATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTcc                                                                                                     <  2:1636238/150‑1 (MQ=255)

                                                 gATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTcc                                                                                                     <  1:488962/150‑1 (MQ=255)

                                                    tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg                                                                                                  <  2:1119148/150‑1 (MQ=255)

                                                    tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg                                                                                                  <  1:1297237/150‑1 (MQ=255)

                                                     aaCACCGCCAGAGATAACAACAATGTTGACAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGCAGTTTACCTGCTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGc                                                                                                 >  1:269886/1‑150 (MQ=255)

                                                      acacCGCCAGAGATAACAACAATGTTTCCAGATATCGCACGCTTCCTCCCGCGCGTAGCCAACGACCAGATAGAGGAGATTACCTGGTACGTAAAGCACTTGACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGcc                                                                                                <  1:285630/150‑1 (MQ=255)

                                                      acacCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGcc                                                                                                <  1:134853/150‑1 (MQ=255)

                                                      acacCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGcc                                                                                                >  1:468796/1‑150 (MQ=255)

                                                       cacCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTACCGCGCTTAGCCAACGACCACATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCa                                                                                               <  2:662062/150‑1 (MQ=255)

                                                         ccGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACTTAAATCACTATACGTACAGTAACGCCATAAAGATATATTGCTACCAGATGTTCGTGGCCAGc                                                                                             >  2:774131/1‑150 (MQ=255)

                                                         ccGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGc                                                                                             <  1:428185/150‑1 (MQ=255)

                                                           gCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCAc                                                                                           <  1:1141602/150‑1 (MQ=255)

                                                                 gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     <  1:717532/150‑1 (MQ=255)

                                                                 gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     >  2:499123/1‑150 (MQ=255)

                                                                 gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     >  1:1191745/1‑150 (MQ=255)

                                                                  aTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgcg                                                                                    <  2:179795/150‑1 (MQ=255)

                                                                      caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                >  2:1290813/1‑150 (MQ=255)

                                                                      caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                >  2:1516694/1‑150 (MQ=255)

                                                                      caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                <  1:26088/150‑1 (MQ=255)

                                                                      caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                <  2:439660/150‑1 (MQ=255)

                                                                        acaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTg                                                                              >  2:694289/1‑150 (MQ=255)

                                                                           aTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGttc                                                                           <  1:1424732/150‑1 (MQ=255)

                                                                            tggtGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTTGTACGGAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGttct                                                                          <  2:1133911/147‑1 (MQ=255)

                                                                              ttGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGttcttc                                                                        <  1:1221553/150‑1 (MQ=255)

                                                                               tGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTcc                                                                       <  2:743169/150‑1 (MQ=255)

                                                                                   aGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTg                                                                   <  2:498007/150‑1 (MQ=255)

                                                                                   aGATATCGCACGCTTCCTACCGCTCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTATATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTg                                                                   <  2:721085/150‑1 (MQ=255)

                                                                                   aGATATCGCACGCTGCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTg                                                                   <  2:1177488/150‑1 (MQ=255)

                                                                                     atatCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCg                                                                 <  1:1224478/150‑1 (MQ=255)

                                                                                      tatCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGt                                                                >  1:1671491/1‑150 (MQ=255)

                                                                                       atCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGAGTGAGGAGTTTACCTGGTACGTCAATCACTTTACGTACAGTACCGCCATCAAGATAGTTTGCTACCAGATGTTCCTGGCCAGCCCGTTCGCGACCCAGTTCTGACGTTGCGTc                                                               >  2:324093/1‑150 (MQ=255)

                                                                                           cACGCTTCCTCCCGCGCTTAGCCAACGCCCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACGTGTTCTTCCGTTGCGTCCAcc                                                           >  2:937359/1‑150 (MQ=255)

                                                                                           cACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  1:1855268/150‑1 (MQ=255)

                                                                                           cACGCTTCCTCCCGCGCTTAGACAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGTCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  2:1624420/150‑1 (MQ=255)

                                                                                           cACGCTTCCTCACGCGCTTAGCCACCGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  2:1834147/150‑1 (MQ=255)

                                                                                             cGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCgg                                                         <  1:1544819/150‑1 (MQ=255)

                                                                                             cGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCgg                                                         <  1:1855405/150‑1 (MQ=255)

                                                                                                ttCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTAGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAAc                                                      <  2:1797466/150‑1 (MQ=255)

                                                                                                    tccCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTg                                                  <  1:1811142/150‑1 (MQ=255)

                                                                                                      ccGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAt                                                >  2:1073006/1‑150 (MQ=255)

                                                                                                         ctattAGACTACGAACAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAtttt                                             <  1:80072/147‑1 (MQ=255)

                                                                                                           cTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTgg                                           <  1:748074/150‑1 (MQ=255)

                                                                                                               gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGACCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAGTTTGGCACg                                       >  1:460552/1‑150 (MQ=255)

                                                                                                               gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACg                                       >  2:875084/1‑150 (MQ=255)

                                                                                                               gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCAGTTGCGTCCAACGGAACGGTGATTTTGGCACg                                       >  2:1318244/1‑150 (MQ=255)

                                                                                                                    cGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACtt                                  >  2:756684/1‑150 (MQ=255)

                                                                                                                       ccAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTAc                               >  2:1618595/1‑150 (MQ=255)

                                                                                                                       ccAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTAc                               >  2:683917/1‑150 (MQ=255)

                                                                                                                           aTTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAAAGTAACGCCATCAAGATGTTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACTGTGATTTTGGCACTGACTTTACCGtt                           >  1:1187690/1‑150 (MQ=255)

                                                                                                                              gaggagTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAAc                        >  1:1657618/1‑150 (MQ=255)

                                                                                                                               aggagTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAAcc                       <  1:771170/150‑1 (MQ=255)

                                                                                                                                  agTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGc                    <  2:831894/150‑1 (MQ=255)

                                                                                                                                     cgttactgTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGcacc                 >  1:1551008/8‑150 (MQ=255)

                                                                                                                                        ccTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACg              <  1:1072713/150‑1 (MQ=255)

                                                                                                                                         cTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGa             <  1:1239172/150‑1 (MQ=255)

                                                                                                                                         cTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGa             <  1:1818055/150‑1 (MQ=255)

                                                                                                                                          tGGTACTTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGAGAACCTGTTCTTCCGTTGAGTCCAACGGAACGGTGAATTTGTCACGTAATTTACCGTTAACCTGCACAAAGAc            >  2:1388458/1‑150 (MQ=255)

                                                                                                                                           ggTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGAcc           >  1:641810/1‑150 (MQ=255)

                                                                                                                                            gTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCa          >  1:1198414/1‑150 (MQ=255)

                                                                                                                                            gTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATATTGCTACAAGATGTTCATGGCCAGCACGTTCGCGAACCTGATCTTCCGTAGCGTCCACCGGAACGGTGATTTTTGCACGGACTTTACCGTTAACCTGCACCACGACCa          >  2:64802/1‑150 (MQ=255)

                                                                                                                                                  aaaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGTCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTgg    <  1:972593/150‑1 (MQ=255)

                                                                                                                                                  aaaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGCCTTTACCGTTAACCTGCACCACGACCCGCGTgg    >  1:367863/1‑150 (MQ=255)

                                                                                                                                                  aaaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTgg    >  1:856168/1‑150 (MQ=255)

                                                                                                                                                   aaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGa   >  1:139694/1‑150 (MQ=255)

                                                                                                                                                    aTCACTTTACGTTCAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAg  <  1:156601/150‑1 (MQ=255)

                                                                                                                                                    aTCACTTTACGTACAGAACCGCCATCAAGATTTTTTGCTCCCCGATGTTCCTGGCCCGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAATTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAg  <  2:354227/150‑1 (MQ=255)

TGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAG  >  NC_000913/672093‑672390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`