BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	672,244	A→G	6.3%	V847A (GTG→GCG) ‡	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,244	0	A	G	6.3%	374.8 / 10.2	125	V847A (GTG→GCG) ‡	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (52/65); new base G (5/3); total (57/68)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 126 total aligned reads displayed.

GAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAG  >  NC_000913/672095‑672390

gaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCa                                                                                                                                                    >  2:1043727/1‑150 (MQ=255)

  aggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt                                                                                                                                                  >  2:1441034/1‑150 (MQ=255)

   ggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACtt                                                                                                                                                 <  2:1830765/150‑1 (MQ=255)

     aaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTa                                                                                                                                               <  2:605899/150‑1 (MQ=255)

      atcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTAc                                                                                                                                              >  2:1072713/3‑150 (MQ=255)

          gCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAc                                                                                                                                          <  2:846406/150‑1 (MQ=255)

          gCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAc                                                                                                                                          >  2:1571441/1‑150 (MQ=255)

           ggttgTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAGTGATTTACGTAcc                                                                                                                                         <  2:946177/146‑2 (MQ=255)

           cGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAGTGATTTACGTAcc                                                                                                                                         <  1:1082657/150‑2 (MQ=255)

              ggTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTATACGTACAgga                                                                                                                                      >  1:354227/1‑148 (MQ=255)

                tATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAAc                                                                                                                                    >  2:106410/1‑150 (MQ=255)

                 aTCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACg                                                                                                                                   <  1:1843788/150‑1 (MQ=255)

                   cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAACTCACTTTACGTACAGTAACGcc                                                                                                                                 >  1:240937/1‑150 (MQ=255)

                   cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGcc                                                                                                                                 >  2:1855268/1‑150 (MQ=255)

                   cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGACAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAACACTTTACGTACAGTAACGcc                                                                                                                                 >  1:350771/1‑150 (MQ=255)

                     cGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCAt                                                                                                                               >  2:748074/1‑150 (MQ=255)

                        aGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCaa                                                                                                                            >  2:771170/1‑150 (MQ=255)

                          aTGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                                                                                                                          <  1:661566/150‑1 (MQ=255)

                           tGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGat                                                                                                                         >  2:972593/1‑150 (MQ=255)

                            gccagccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGata                                                                                                                        <  1:1126330/150‑1 (MQ=255)

                            gccagccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGata                                                                                                                        >  2:1201616/1‑150 (MQ=255)

                              gcgccaCACCCGGCGGTGATTACCACCGCCAGAGATAACAACAATGGTGCCAGATATCGCACGCTTCCTCCCGCGCTTCGCCAACGCCCAGATTGAGGTGTTTACCTGGTACGTAAATCACTGTCCGTCCAGTCACGCCAGCAAGATAtt                                                                                                                      >  1:1477626/3‑150 (MQ=255)

                                gccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATAtttt                                                                                                                    <  1:157499/150‑1 (MQ=255)

                                 ccaCACCCGGCGGTGATTAACACCGCCAGAGATAAGAACAATGTTGAGAGATATCGCACGCTTCCTCCCGCGCTTACCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTg                                                                                                                   <  2:755028/150‑1 (MQ=255)

                                 ccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTg                                                                                                                   <  2:280871/150‑1 (MQ=255)

                                   acacCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCt                                                                                                                 <  2:1083049/150‑1 (MQ=255)

                                      cccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc                                                                                                              <  1:459415/150‑1 (MQ=255)

                                       ccGGCGGTTATTAACACCGCCAGAGATAACACCAATGTTGCCAGATATCGCACGCTTCCTCCCTCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGAAATTTTGCTACCa                                                                                                             <  2:1498175/150‑1 (MQ=255)

                                       ccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGACAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCa                                                                                                             <  1:848707/150‑1 (MQ=255)

                                        ggggggTGTTTAACACCTCAAGAGATCACAACAATGTTGCGAGATATAGCACGCTTCCTCCCGCGGTTAGCCACCGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCCTCAAGTTATTTTGCTACCAg                                                                                                            <  2:330393/146‑1 (MQ=255)

                                         ggcggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGa                                                                                                           <  1:810201/150‑1 (MQ=255)

                                            ggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGt                                                                                                        <  2:1744369/150‑1 (MQ=255)

                                             gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGtt                                                                                                       >  1:827453/1‑150 (MQ=255)

                                             gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGtt                                                                                                       <  1:1750861/150‑1 (MQ=255)

                                               gATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTcc                                                                                                     <  2:1636238/150‑1 (MQ=255)

                                               gATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTcc                                                                                                     <  1:488962/150‑1 (MQ=255)

                                                  tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg                                                                                                  <  2:1119148/150‑1 (MQ=255)

                                                  tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg                                                                                                  <  1:1297237/150‑1 (MQ=255)

                                                   aaCACCGCCAGAGATAACAACAATGTTGACAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGCAGTTTACCTGCTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGc                                                                                                 >  1:269886/1‑150 (MQ=255)

                                                    acacCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTACATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGcc                                                                                                >  1:349591/1‑150 (MQ=255)

                                                       ccGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGc                                                                                             <  1:428185/150‑1 (MQ=255)

                                                        cGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGGTCCTGGCCAGCa                                                                                            <  2:1657956/150‑1 (MQ=255)

                                                         gCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCAc                                                                                           <  1:1141602/150‑1 (MQ=255)

                                                               gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     >  1:1191745/1‑150 (MQ=255)

                                                               gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     <  1:717532/150‑1 (MQ=255)

                                                               gaTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgc                                                                                     >  2:499123/1‑150 (MQ=255)

                                                                aTAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTcgcg                                                                                    <  2:179795/150‑1 (MQ=255)

                                                                   acaacaATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAc                                                                                 <  1:1441034/150‑1 (MQ=255)

                                                                    caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                >  2:1290813/1‑150 (MQ=255)

                                                                    caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                >  2:1516694/1‑150 (MQ=255)

                                                                    caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                <  1:26088/150‑1 (MQ=255)

                                                                    caacaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAAcc                                                                                <  2:439660/150‑1 (MQ=255)

                                                                      acaaTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTg                                                                              >  2:694289/1‑150 (MQ=255)

                                                                         aTGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGttc                                                                           <  1:1424732/150‑1 (MQ=255)

                                                                          tggtGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTTGTACGGAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGttct                                                                          <  2:1133911/147‑1 (MQ=255)

                                                                             tGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTcc                                                                       <  1:1201616/150‑1 (MQ=255)

                                                                                 aGATATCGCACGCTTCCTACCGCTCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTATATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTg                                                                   <  2:721085/150‑1 (MQ=255)

                                                                                 aGATATCGCACGCTGCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTg                                                                   <  2:1177488/150‑1 (MQ=255)

                                                                                   atatCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCg                                                                 <  1:1224478/150‑1 (MQ=255)

                                                                                    tatCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGt                                                                >  1:1671491/1‑150 (MQ=255)

                                                                                       cGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCa                                                             >  2:691968/1‑150 (MQ=255)

                                                                                        gCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAc                                                            >  1:668752/1‑150 (MQ=255)

                                                                                         cACGCTTCCTCCCGCGCTTAGCCAACGCCCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACGTGTTCTTCCGTTGCGTCCAcc                                                           >  2:937359/1‑150 (MQ=255)

                                                                                         cACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  1:1855268/150‑1 (MQ=255)

                                                                                         cACGCTTCCTCCCGCGCTTAGACAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGTCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  2:1624420/150‑1 (MQ=255)

                                                                                         cACGCTTCCTCACGCGCTTAGCCACCGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCAcc                                                           <  2:1834147/150‑1 (MQ=255)

                                                                                              ttCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTAGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAAc                                                      <  2:1797466/150‑1 (MQ=255)

                                                                                                  tccCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTg                                                  <  1:1811142/150‑1 (MQ=255)

                                                                                                         cTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTgg                                           <  1:748074/150‑1 (MQ=255)

                                                                                                             gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGACCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAGTTTGGCACg                                       >  1:460552/1‑150 (MQ=255)

                                                                                                             gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACg                                       >  2:875084/1‑150 (MQ=255)

                                                                                                             gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACg                                       >  1:467574/1‑150 (MQ=255)

                                                                                                             gCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCAGTTGCGTCCAACGGAACGGTGATTTTGGCACg                                       >  2:1318244/1‑150 (MQ=255)

                                                                                                               cAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGa                                     >  2:802655/1‑150 (MQ=255)

                                                                                                                  cGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACtt                                  >  2:756684/1‑150 (MQ=255)

                                                                                                                     ccAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTAc                               >  2:683917/1‑150 (MQ=255)

                                                                                                                       aGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCg                             <  1:761149/150‑1 (MQ=255)

                                                                                                                         aTTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGtt                           <  1:1688379/150‑1 (MQ=255)

                                                                                                                         aTTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTAAAGTAACGCCATCAAGATGTTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACTGTGATTTTGGCACTGACTTTACCGtt                           >  1:1187690/1‑150 (MQ=255)

                                                                                                                             aggagTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAAcc                       <  1:771170/150‑1 (MQ=255)

                                                                                                                                agTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGc                    <  2:831894/150‑1 (MQ=255)

                                                                                                                                   cgttactgTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGcacc                 >  1:1551008/8‑150 (MQ=255)

                                                                                                                                      ccTGGTACGTAAATCACTTTACGTACAGTAACGCCATCATGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACg              <  1:1571441/150‑1 (MQ=255)

                                                                                                                                      ccTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAGCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGCCTTTACCGTTAACCTGCACCACg              >  1:225755/1‑150 (MQ=255)

                                                                                                                                      ccTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACg              <  1:1072713/150‑1 (MQ=255)

                                                                                                                                       cTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGa             <  1:1239172/150‑1 (MQ=255)

                                                                                                                                       cTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCCTTGCGTCAACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGa             >  1:133088/1‑150 (MQ=255)

                                                                                                                                        tGGTACTTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGAGAACCTGTTCTTCCGTTGAGTCCAACGGAACGGTGAATTTGTCACGTAATTTACCGTTAACCTGCACAAAGAc            >  2:1388458/1‑150 (MQ=255)

                                                                                                                                         tgTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGAcc           >  2:432690/2‑150 (MQ=255)

                                                                                                                                         ggTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGAcc           >  1:641810/1‑150 (MQ=255)

                                                                                                                                          gTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCa          >  1:1198414/1‑150 (MQ=255)

                                                                                                                                          gTACGTAAAGTGATTTACGTACAGTAACGCCATCAAGATATATTGCTACAAGATGTTCATGGCCAGCACGTTCGCGAACCTGATCTTCCGTAGCGTCCACCGGAACGGTGATTTTTGCACGGACTTTACCGTTAACCTGCACCACGACCa          >  2:64802/1‑150 (MQ=255)

                                                                                                                                             cGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCg       >  2:590244/1‑150 (MQ=255)

                                                                                                                                                aaaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGTCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTgg    <  1:972593/150‑1 (MQ=255)

                                                                                                                                                aaaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGCCTTTACCGTTAACCTGCACCACGACCCGCGTgg    >  1:367863/1‑150 (MQ=255)

                                                                                                                                                aaaTCACTTAACCTACAGTTACGCCATCAAGATATTTTGCTACCAGAGGTTCCTGGCCAGCACGATCGCGAACCTGTTCTTCCGTTGCGGCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCgggg    <  2:269174/150‑1 (MQ=255)

                                                                                                                                                 aaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGa   >  1:1834855/1‑150 (MQ=255)

                                                                                                                                                 aaTCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGa   >  1:139694/1‑150 (MQ=255)

                                                                                                                                                  aTCACTTTACGTTCAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAg  <  1:156601/150‑1 (MQ=255)

                                                                                                                                                  aTCACTTTACGTACAGAACCGCCATCAAGATTTTTTGCTCCCCGATGTTCCTGGCCCGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGAATTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAg  <  2:354227/150‑1 (MQ=255)

GAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAG  >  NC_000913/672095‑672390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`