Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,066 T→G 6.3% D35A (GAC→GCC) ‡ ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0660TG 6.3% 374.1 / 8.6 139D35A (GAC→GCC) ‡ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (64/65);  new base G (3/7);  total (67/72)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

CATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCC  >  NC_000913/751917‑752215
                                                                                                                                                     |                                                                                                                                                     
cATTGATGCATGGAATGGATTTGTCACCCTTTTTTTGCCAGTTAAATTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTAACATGGAGCAATGCCTCCTTTTCCTATAATAATGAAATATCAATGCGGTc                                                                                                                                                      >  1:1025992/1‑150 (MQ=255)
aaTTTATGCATTGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGt                                                                                                                                                       <  1:880970/149‑1 (MQ=255)
    taTGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                   <  1:776552/149‑1 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                   <  2:838332/150‑1 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                   >  1:1223932/1‑150 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGAATCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcc                                                                                                                                                   >  1:1310021/1‑149 (MQ=255)
     aTGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCgcgc                                                                                                                                                  <  2:30551/150‑1 (MQ=255)
      tGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCa                                                                                                                                                 >  2:1086583/1‑150 (MQ=255)
      tGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCa                                                                                                                                                 >  2:393025/1‑150 (MQ=255)
       gCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAt                                                                                                                                                >  1:1299239/1‑150 (MQ=255)
        cATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                               >  1:553924/1‑150 (MQ=255)
        cATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                               >  1:157409/1‑150 (MQ=255)
           ggAATGGTTTTGTCACCCTTTTTTTGACAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTATTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAt                                                                                                                                            <  1:85477/150‑1 (MQ=255)
            taaTGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAta                                                                                                                                           <  1:1812376/149‑2 (MQ=255)
            gAATGGGTTTGTCACCCGTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAta                                                                                                                                           <  1:334399/150‑2 (MQ=255)
                ggTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATc                                                                                                                                       <  1:1401444/150‑1 (MQ=255)
                ggTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTACTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATc                                                                                                                                       <  1:1692582/150‑1 (MQ=255)
                 gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCTGGAGCAATTACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATct                                                                                                                                      >  1:1163357/1‑149 (MQ=255)
                 gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGATCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCg                                                                                                                                      <  1:1747959/150‑1 (MQ=255)
                 gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCg                                                                                                                                      <  1:1807964/150‑1 (MQ=255)
                   tttGTCACACTTTTTTTGCCAGTTAATTTTTTGCCCATTTCTGATTTTGTTGTTATTCACCTCAACGCTAACAAAATATTCACAGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCTCATTGATTTATCgag                                                                                                                                    <  2:65004/150‑1 (MQ=255)
                    ttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgaga                                                                                                                                   <  2:1802420/150‑1 (MQ=255)
                         aCCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAtt                                                                                                                              <  2:695011/150‑1 (MQ=255)
                          cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                             >  1:845589/1‑150 (MQ=255)
                          cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                             <  2:1327582/150‑1 (MQ=255)
                           ccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCa                                                                                                                            >  1:296158/1‑150 (MQ=255)
                           ccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCa                                                                                                                            <  2:896322/150‑1 (MQ=255)
                               tttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAtt                                                                                                                        >  1:745505/1‑150 (MQ=255)
                               tttttGCCAGTAAATTTTATGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAtt                                                                                                                        <  2:1728792/150‑1 (MQ=255)
                                ttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTg                                                                                                                       <  2:1858950/150‑1 (MQ=255)
                                    gCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACAGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATTCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAtt                                                                                                                   >  2:1569002/1‑150 (MQ=255)
                                        gTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCAGCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGAc                                                                                                               <  1:308964/150‑1 (MQ=255)
                                            aTTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGcc                                                                                                           <  2:943271/150‑1 (MQ=255)
                                            aTTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGcc                                                                                                           >  1:1009771/1‑150 (MQ=255)
                                                  tGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGAGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTgcg                                                                                                     >  1:1865853/1‑150 (MQ=255)
                                                   gCCCATTACTGATATTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATAAAAGCCTTCGCGCATTGATTTATCGATAACATTCAGATTTAAATAGACCGCCATTgcgc                                                                                                    >  2:640520/1‑150 (MQ=255)
                                                    cccATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCa                                                                                                   <  2:1299239/150‑1 (MQ=255)
                                                    cccATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCa                                                                                                   <  2:1686424/150‑1 (MQ=255)
                                                         tACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCa                                                                                              >  2:614030/1‑150 (MQ=255)
                                                           cTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATc                                                                                            >  2:503173/1‑150 (MQ=255)
                                                            tGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCg                                                                                           <  1:1448134/150‑1 (MQ=255)
                                                               ttttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCa                                                                                        >  2:392444/1‑150 (MQ=255)
                                                                 ttgttgTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATg                                                                                      <  1:1554674/150‑1 (MQ=255)
                                                                     tgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACca                                                                                  >  1:1757986/1‑150 (MQ=255)
                                                                     tgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACca                                                                                  <  1:1351490/150‑1 (MQ=255)
                                                                      gttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcag                                                                                 >  2:1461544/1‑150 (MQ=255)
                                                                        tATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggc                                                                               >  2:1557444/1‑150 (MQ=255)
                                                                        tATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggc                                                                               <  2:1486149/150‑1 (MQ=255)
                                                                         aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGGCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGATCATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCAGGACcaggca                                                                              >  2:1067927/1‑150 (MQ=255)
                                                                         aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                              >  2:1205853/1‑150 (MQ=255)
                                                                           tCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggcagg                                                                            <  1:934230/150‑1 (MQ=255)
                                                                            cACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGa                                                                           <  1:16676/150‑1 (MQ=255)
                                                                             aCCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAt                                                                          >  2:647374/1‑150 (MQ=255)
                                                                              ccGCAACGCTAACAAAATATTCACCGGGAGCTATGACTGCTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCTTGATAc                                                                        <  2:1025992/150‑1 (MQ=255)
                                                                              ccGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATa                                                                         <  1:393025/150‑1 (MQ=255)
                                                                              ccGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCACTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATa                                                                         <  2:1679754/150‑1 (MQ=255)
                                                                               cGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCGTTTTTCCTTTATTAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGTCCGCCATTGCGCAAGGCATCGCCATGACGAGGCAGGATAc                                                                        >  2:146696/1‑150 (MQ=255)
                                                                                  caagCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAGTTATCGAGAACATACAGATTGAATTCGTCCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACaaa                                                                     >  2:72415/4‑150 (MQ=255)
                                                                                   aCGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACaaaa                                                                    >  2:1747191/1‑150 (MQ=255)
                                                                                    cGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAag                                                                   <  1:1828872/150‑1 (MQ=255)
                                                                                    cGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAag                                                                   <  1:1821760/150‑1 (MQ=255)
                                                                                       tAACAAAATATTCACCGGTACCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCTGTCGCGCATTGATTTATCGATAACATTCAGATTGTATTCGACCGCCATTGCGCAATGCATCGCCATGACCAGGCAGGATACAAAagaga                                                                <  1:1388699/150‑1 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGCCCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                               >  2:572630/1‑150 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                               >  2:336821/1‑150 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTACTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                               <  1:1179754/150‑1 (MQ=255)
                                                                                          aaaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTc                                                             <  1:559988/149‑1 (MQ=255)
                                                                                             aaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGAt                                                          <  2:296158/150‑1 (MQ=255)
                                                                                              atatTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                         <  2:533456/150‑1 (MQ=255)
                                                                                              atatTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                         <  2:1223932/150‑1 (MQ=255)
                                                                                                  tCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGTACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGGCGATAAata                                                     <  1:278380/150‑1 (MQ=255)
                                                                                                  tCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAata                                                     <  1:1095432/150‑1 (MQ=255)
                                                                                                     ccGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTc                                                  >  2:464034/1‑150 (MQ=255)
                                                                                                       gggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                                >  1:1032815/1‑150 (MQ=255)
                                                                                                       gggAGCAAAGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                                >  2:1034650/1‑150 (MQ=255)
                                                                                                         gAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACgg                                              <  1:386662/150‑1 (MQ=255)
                                                                                                           gCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCTCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                            <  1:34011/150‑1 (MQ=255)
                                                                                                            cAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtgt                                           >  1:346230/1‑150 (MQ=255)
                                                                                                               tGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCa                                        <  2:1263643/150‑1 (MQ=255)
                                                                                                                   tCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATAcc                                    <  2:1831183/150‑1 (MQ=255)
                                                                                                                     cTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGCCCAGGCAGGATACAAAAGAGAGTCGATAAATTTTCACGGTTTCCATACCTg                                  >  2:1128646/1‑150 (MQ=255)
                                                                                                                     cTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                               >  1:1770307/1‑121 (MQ=255)
                                                                                                                        tttCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATa                               <  1:1746089/150‑1 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGCCCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTTTCCATACCGGATAAata                           >  1:940237/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                           >  1:1454041/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                           >  1:380071/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                           >  2:648086/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                           >  1:965813/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                           >  2:480804/1‑150 (MQ=255)
                                                                                                                               taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAttt                        >  1:1770868/1‑150 (MQ=255)
                                                                                                                                aataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAtttt                       <  2:1310021/150‑1 (MQ=255)
                                                                                                                                 ataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTa                      <  1:1001076/150‑1 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACTTGATAAATATTTTATg                    <  2:829246/150‑1 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                    >  2:1500178/1‑150 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAAACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                    <  2:157409/150‑1 (MQ=255)
                                                                                                                                    atGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGa                   <  2:528993/150‑1 (MQ=255)
                                                                                                                                     cgataaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATATATATTCACGGTGTCCATACCTGATAAATATTTTATGaa                  >  2:1734074/7‑150 (MQ=255)
                                                                                                                                      gataaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGaaa                 >  2:1853680/6‑150 (MQ=255)
                                                                                                                                      gAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATCCCTGATAAATATTTTATGaaa                 >  1:278849/1‑150 (MQ=255)
                                                                                                                                                  cGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatgat     >  2:1109032/1‑150 (MQ=255)
                                                                                                                                                  cGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatgat     >  1:1555977/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCC  >  NC_000913/751917‑752215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: