Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 794,938 C→A 5.6% intergenic (+16/‑151) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,9380CA 5.6% 341.4 / 6.9 120intergenic (+16/‑151)acrZ/modAAcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
Reads supporting (aligned to +/- strand):  ref base C (59/54);  new base A (3/4);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 122 total aligned reads displayed.

AAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794790‑795087
                                                                                                                                                    |                                                                                                                                                     
acgTCTGGTATTCGCCGTAATCATTGTACCTGTCGTGATGGCCATCATCGTGGGGCTGATTTACGGTCTTGGGGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCt                                                                                                                                                      <  2:112754/148‑1 (MQ=255)
  gTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGTTCTTGGTGAAGTATTCAACATCTATTCTGGTGTTGGTAAAAAAGACAAGCCCGGACAAAATCATTGATTCCCTGAATGACCGCTTa                                                                                                                                                    >  2:109823/1‑150 (MQ=255)
    cTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGt                                                                                                                                                  >  2:874787/1‑150 (MQ=255)
      ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                >  2:124938/1‑150 (MQ=255)
        tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                              >  1:1225191/1‑150 (MQ=255)
        tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                              >  1:1597790/1‑150 (MQ=255)
         aTTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGc                                                                                                                                             >  1:152642/1‑150 (MQ=255)
              ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCCtttt                                                                                                                                        >  1:1340525/1‑150 (MQ=255)
               cGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcag                                                                                                                                       <  1:105342/150‑4 (MQ=255)
                gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                      <  1:575370/150‑5 (MQ=255)
                gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                      <  2:705993/150‑5 (MQ=255)
                gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGACCGCTTAGTCGGGCAttttct                                                                                                                                      >  1:732167/1‑150 (MQ=255)
                gTAATCATGGTACCTGTCGAGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttct                                                                                                                                      <  1:354208/150‑1 (MQ=255)
                gTAATCATGGTACCTGGCGTGATGGCCATCATCCTGGGTCTGATTTACGGGCTTGGGGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                      <  2:1690611/150‑5 (MQ=255)
                  aaTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttcttt                                                                                                                                    <  2:1694452/150‑1 (MQ=255)
                   aTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                   <  1:1835878/150‑1 (MQ=255)
                   aTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                   >  1:130727/1‑150 (MQ=255)
                   aTCATGGTACCTGGCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                   <  2:1611874/150‑1 (MQ=255)
                     cATGGTAACTGTCTTGATGGCCATCATACTGTGTCTTATTTACGGTCTTTGTGAAGTATTCAACATATTTTCTGGTGTTGGTAAAAAATACCAGACCGGACAAAATAATTGATTCCCTGAATGACATCTTAGTCGGGCAgtttattttta                                                                                                                                 >  2:887384/1‑149 (MQ=255)
                      aTGGTACCTGTCTGGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttttct                                                                                                                                <  1:1006693/150‑1 (MQ=255)
                       tGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTtctc                                                                                                                               <  1:342314/150‑1 (MQ=255)
                       tGGTACCTGTCGTGATGGCCAGCATCCTGGGTCTGATTTACGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTtctc                                                                                                                               <  2:1630410/150‑1 (MQ=255)
                             cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTATTCGGGCATTTTCTTTTTCTCAACtta                                                                                                                         >  1:1172091/1‑149 (MQ=255)
                               gTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCt                                                                                                                       >  1:464456/1‑150 (MQ=255)
                                 cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGc                                                                                                                     <  1:193402/150‑1 (MQ=255)
                                 cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGc                                                                                                                     >  2:885771/1‑150 (MQ=255)
                                    gATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCttt                                                                                                                  <  2:1473627/150‑1 (MQ=255)
                                    gATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCttt                                                                                                                  >  2:1773401/1‑150 (MQ=255)
                                       ggCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCACTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTcc                                                                                                               >  1:911933/1‑150 (MQ=255)
                                         ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCATCTTCCTGCTTTTCCTg                                                                                                             >  1:101681/1‑150 (MQ=255)
                                         ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTg                                                                                                             >  2:507510/1‑150 (MQ=255)
                                         ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTg                                                                                                             >  2:1542835/1‑150 (MQ=255)
                                           atcatcCTGGGTCTGATTGACGGTCTTGGTGAAGTCTTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAACTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                           <  2:1819234/150‑1 (MQ=255)
                                            tcatcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCg                                                                                                          <  2:442788/150‑1 (MQ=255)
                                              atcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGat                                                                                                        >  1:1752262/1‑150 (MQ=255)
                                                    ggTCTGATTTACTGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                  <  1:188935/150‑1 (MQ=255)
                                                     gTCTGCTTGACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGCTTCCCTGAATGCCAGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                 <  2:1310833/150‑1 (MQ=255)
                                                      tCTGATTTACTGTCTTGTAGAAGTATTAAACATCATTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTCATTCCCTGAATTCCCGCTAAGTCGGGCATTTTCTTTTTCTAAACTTCCTGCTTTTCCTGCCGATAtttttta                                                                                                >  2:1342676/1‑149 (MQ=255)
                                                      tCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                <  1:547939/150‑1 (MQ=255)
                                                      tCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                <  1:443595/150‑1 (MQ=255)
                                                      gcTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACCAAATCATTGATTCCCTGAATGCCCGCTTAGGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                <  2:1206588/149‑1 (MQ=255)
                                                       gtGATTTACGGTCTTGGTGAAGTATTCATCATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  2:373412/149‑1 (MQ=255)
                                                       gtGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  2:1397446/149‑1 (MQ=255)
                                                       cTGATTTTCGGTCTTGTTGAAGTATTCAACAGCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGCCCAAACTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  2:1157764/150‑1 (MQ=255)
                                                       cTGATTTACGGTCTTGGTGAATTATTCAACATCTTTTCTGTTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTACCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  1:1260880/150‑1 (MQ=255)
                                                       cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  2:676419/150‑1 (MQ=255)
                                                           tttACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATc                                                                                           >  2:1286546/1‑150 (MQ=255)
                                                            ttACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTTTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCt                                                                                          <  2:336668/150‑1 (MQ=255)
                                                             tACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTa                                                                                         >  2:1645208/1‑150 (MQ=255)
                                                              aCGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAATAGACGAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAc                                                                                        <  1:70671/150‑1 (MQ=255)
                                                               cGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTACCTGCTTTTCCTGCCGATATTTTTTCTTATATAcc                                                                                       >  2:1535111/1‑150 (MQ=255)
                                                                         gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                             >  2:600247/1‑150 (MQ=255)
                                                                         gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                             >  2:1174310/1‑150 (MQ=255)
                                                                           aGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTa                                                                           <  1:898570/150‑1 (MQ=255)
                                                                            gTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAAACATTGATTCACTGAATGACCGCTTAGTCCGGCATTATCTTTTTATCAACTTCCTGCTTTTCCTGCCGAAATTTTTTCTTATCTACCTCACAAATGTTAg                                                                          >  1:800118/1‑150 (MQ=255)
                                                                             tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATGCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                         <  1:300542/150‑1 (MQ=255)
                                                                              aTTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCa                                                                        >  1:512961/1‑150 (MQ=255)
                                                                              aTTCAACATCTTTTCTGGTGTAGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCa                                                                        <  2:130117/150‑1 (MQ=255)
                                                                                 cAACATCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  2:595635/1‑150 (MQ=255)
                                                                                 cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  1:1318837/1‑150 (MQ=255)
                                                                                 cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  1:1455596/1‑150 (MQ=255)
                                                                                 cAACATCTTTTCTGGTGTTGGTAAAAAAGACAAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTAAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  2:906994/1‑150 (MQ=255)
                                                                                     aTCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActg                                                                 <  1:90236/150‑1 (MQ=255)
                                                                                      tCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgc                                                                >  1:1582258/1‑150 (MQ=255)
                                                                                       cTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTACTGCTTTTCCTGCCGATATTTTTTCTTATATACCTCACAAAGGTTAGCAATAActgat                                                               >  2:1419559/1‑148 (MQ=255)
                                                                                         tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                             <  1:574132/150‑1 (MQ=255)
                                                                                         tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                             <  1:23943/150‑1 (MQ=255)
                                                                                             tGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGaaa                                                         >  1:723677/1‑150 (MQ=255)
                                                                                                 gtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCATGAATGCCCGCTTTGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCAGGCCGATATTTTTTCTTATCTAGCTCCCAAAGGTTAGCAATAACTGCTGGGATAATTc                                                     >  2:1324255/1‑150 (MQ=255)
                                                                                                    ggTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGa                                                  >  1:715101/1‑150 (MQ=255)
                                                                                                     gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCAAAAAGGTTAGCAATAACTTCTGGGAAAATTCCTAg                                                 >  2:150533/1‑150 (MQ=255)
                                                                                                         aaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAg                                             >  2:531815/1‑150 (MQ=255)
                                                                                                           aaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTc                                           <  1:1359095/150‑1 (MQ=255)
                                                                                                              aCCAGCACGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGtt                                        <  1:822646/150‑1 (MQ=255)
                                                                                                               ccagccagGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTATTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTta                                       <  1:915456/150‑1 (MQ=255)
                                                                                                               ccAGCACGGACAAACTCATTGATTCCCTGAATGCCCGCTGAGTCGGGCATTTTCGTTTTCGCAAGTTGCTGCGGTTCCGGCCGATATGTTTGCTTATCTGCCGCTCATAGGTTAGCGATAGCTGCTGGGAATATTCCGAGTGAGTCGGta                                       >  2:177442/1‑150 (MQ=255)
                                                                                                                 atcccGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCTATATTTTTTCTTATCTACCTCACAAAGGTTAGCAAACACTGCTGGGAAAATTCCGAGTTAGGCGTtata                                     <  2:1433065/148‑1 (MQ=255)
                                                                                                                       taCAAAATCATTGATTCCCTGAATGCCCGCTTAGTCTGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                               <  1:244224/149‑1 (MQ=255)
                                                                                                                       gACAAAATCATTGATTCCCTGTATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                               <  1:491748/150‑1 (MQ=255)
                                                                                                                               cATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATa                       <  2:604310/150‑1 (MQ=255)
                                                                                                                                aTTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTTCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTATTCGTTATATTGTCGCCTACATaa                      <  2:732167/150‑1 (MQ=255)
                                                                                                                                  tGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCCCAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACg                    >  2:1776229/1‑150 (MQ=255)
                                                                                                                                   gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                   >  1:698045/1‑150 (MQ=255)
                                                                                                                                   gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                   <  2:419552/150‑1 (MQ=255)
                                                                                                                                     ttCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTa                 <  1:596185/150‑1 (MQ=255)
                                                                                                                                     ttCCCTGAATGCCCGCATAGGCGGGCATTTTCTTTTTCTCAACTTCCTGCTATTCCTGCCGATATATTTTCTTATCTACCTCACAATGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTa                 <  1:109823/150‑1 (MQ=255)
                                                                                                                                       cccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa               >  1:1545713/1‑150 (MQ=255)
                                                                                                                                        ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGAAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTTTATTGTCGCCTACATAACGTTACAt              >  1:1626747/1‑150 (MQ=255)
                                                                                                                                        ccTGAATGACCGCTTAGTCGGGCATTTTATTTTTCTCAAATTAATGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTGGTTATATTGTCGCCTACATAACGTTAGAt              >  2:1146600/1‑150 (MQ=255)
                                                                                                                                         gaaaaTGCCCGACTAAGCGGGCATTTTCTTTTACTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAtt             >  1:300061/4‑150 (MQ=255)
                                                                                                                                         cTGAATGCCCGCTTAGTCGTGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAtt             <  1:885771/150‑1 (MQ=255)
                                                                                                                                             aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTTCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACCGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         <  1:1419742/150‑1 (MQ=255)
                                                                                                                                             aTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         >  1:174297/1‑150 (MQ=255)
                                                                                                                                               gCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg       >  1:1705316/1‑150 (MQ=255)
                                                                                                                                               gCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg       <  1:1255829/150‑1 (MQ=255)
                                                                                                                                               gCCCGCTTAGTCCGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg       <  2:343096/150‑1 (MQ=255)
                                                                                                                                                  cGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTa    >  1:380222/1‑150 (MQ=255)
                                                                                                                                                  cGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCATATATACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGGTATATTGTCGCCTACATAAAGTTACATTAAGGGGTTa    >  2:362606/1‑150 (MQ=255)
                                                                                                                                                   gCTTATTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAc   <  1:874787/150‑1 (MQ=255)
                                                                                                                                                    cTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc  <  1:1752112/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794790‑795087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: