Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 794,943 T→G 6.8% intergenic (+21/‑146) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,9430TG 6.8% 367.7 / 11.3 122intergenic (+21/‑146)acrZ/modAAcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
Reads supporting (aligned to +/- strand):  ref base T (58/55);  new base G (3/6);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 124 total aligned reads displayed.

CTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATGG  >  NC_000913/794794‑795092
                                                                                                                                                     |                                                                                                                                                     
  ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                     >  2:124938/1‑150 (MQ=255)
    tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                                   >  1:1597790/1‑150 (MQ=255)
    tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                                   >  1:1225191/1‑150 (MQ=255)
     aTTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGc                                                                                                                                                  >  1:152642/1‑150 (MQ=255)
          ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCCtttt                                                                                                                                             >  1:1340525/1‑150 (MQ=255)
          ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCACGCTTAGTCGGGCCtttt                                                                                                                                             >  1:1334049/1‑150 (MQ=255)
           cGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcag                                                                                                                                            <  1:105342/150‑4 (MQ=255)
            gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttct                                                                                                                                           <  1:683466/150‑1 (MQ=255)
            gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                           <  1:575370/150‑5 (MQ=255)
            gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                           <  2:705993/150‑5 (MQ=255)
            gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGACCGCTTAGTCGGGCAttttct                                                                                                                                           >  1:732167/1‑150 (MQ=255)
            gTAATCATGGTACCTGTCGAGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttct                                                                                                                                           <  1:354208/150‑1 (MQ=255)
            gTAATCATGGTACCTGGCGTGATGGCCATCATCCTGGGTCTGATTTACGGGCTTGGGGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                           <  2:1690611/150‑5 (MQ=255)
              aaTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttcttt                                                                                                                                         <  2:1694452/150‑1 (MQ=255)
               aTCATGGTACCTGGCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                        <  2:1611874/150‑1 (MQ=255)
                 cATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTTGTGAAGTATTAAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTTAATGCCCTCTTAGTCGGGGAttttcttttta                                                                                                                                      >  2:1467679/1‑149 (MQ=255)
                 cATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAATAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttttc                                                                                                                                      <  2:1583447/150‑1 (MQ=255)
                 cATGGTAACTGTCTTGATGGCCATCATACTGTGTCTTATTTACGGTCTTTGTGAAGTATTCAACATATTTTCTGGTGTTGGTAAAAAATACCAGACCGGACAAAATAATTGATTCCCTGAATGACATCTTAGTCGGGCAgtttattttta                                                                                                                                      >  2:887384/1‑149 (MQ=255)
                  aTGGTACCTGTCTGGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttttct                                                                                                                                     <  1:1006693/150‑1 (MQ=255)
                   tGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTtctc                                                                                                                                    <  1:342314/150‑1 (MQ=255)
                   tGGTACCTGTCGTGATGGCCAGCATCCTGGGTCTGATTTACGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTtctc                                                                                                                                    <  2:1630410/150‑1 (MQ=255)
                    ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCa                                                                                                                                   <  2:440814/150‑1 (MQ=255)
                         cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTATTCGGGCATTTTCTTTTTCTCAACtta                                                                                                                              >  1:1172091/1‑149 (MQ=255)
                           gTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCt                                                                                                                            >  1:464456/1‑150 (MQ=255)
                             cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGc                                                                                                                          <  1:193402/150‑1 (MQ=255)
                             cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGc                                                                                                                          >  2:885771/1‑150 (MQ=255)
                                gATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCttt                                                                                                                       <  2:1473627/150‑1 (MQ=255)
                                gATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCttt                                                                                                                       >  2:1773401/1‑150 (MQ=255)
                                   ggCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCACTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTcc                                                                                                                    >  1:911933/1‑150 (MQ=255)
                                     ccATCATCCTGGGTCTGATTTACGTTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTg                                                                                                                  <  2:595937/150‑1 (MQ=255)
                                     ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCATCTTCCTGCTTTTCCTg                                                                                                                  >  1:101681/1‑150 (MQ=255)
                                     ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTg                                                                                                                  >  2:1542835/1‑150 (MQ=255)
                                       atcatcCTGGGTCTGATTGACGGTCTTGGTGAAGTCTTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAACTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                                <  2:1819234/150‑1 (MQ=255)
                                        tcatcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCg                                                                                                               <  2:442788/150‑1 (MQ=255)
                                          atcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGat                                                                                                             >  1:1752262/1‑150 (MQ=255)
                                           tcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGata                                                                                                            >  2:211107/1‑150 (MQ=255)
                                                ggTCTGATTTACTGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                       <  1:188935/150‑1 (MQ=255)
                                                  tCTGATTTACTGTCTTGTAGAAGTATTAAACATCATTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTCATTCCCTGAATTCCCGCTAAGTCGGGCATTTTCTTTTTCTAAACTTCCTGCTTTTCCTGCCGATAtttttta                                                                                                     >  2:1342676/1‑149 (MQ=255)
                                                  tCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                     <  1:443595/150‑1 (MQ=255)
                                                  tCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                     <  1:547939/150‑1 (MQ=255)
                                                   gtGATTTACGGTCTTGGTGAAGTATTCATCATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                    <  2:373412/149‑1 (MQ=255)
                                                   gtGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                    <  2:1397446/149‑1 (MQ=255)
                                                   cTGATTTTCGGTCTTGTTGAAGTATTCAACAGCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGCCCAAACTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                    <  2:1157764/150‑1 (MQ=255)
                                                   cTGATTTACGGTCTTGGTGAATTATTCAACATCTTTTCTGTTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTACCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                    <  1:1260880/150‑1 (MQ=255)
                                                   cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                    <  2:676419/150‑1 (MQ=255)
                                                       tttACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATc                                                                                                >  2:1286546/1‑150 (MQ=255)
                                                        ttACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTTTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCt                                                                                               <  2:336668/150‑1 (MQ=255)
                                                          aCGGTCTTTGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAATAGACGAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAc                                                                                             <  1:70671/150‑1 (MQ=255)
                                                                     gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                                  >  2:600247/1‑150 (MQ=255)
                                                                     gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                                  >  2:1174310/1‑150 (MQ=255)
                                                                     gAAGTATTCAACATCTTTTCTGGTGATGGTAAAAAAGACCAGCCCGGACATAATCATTGATTCCATGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGTTATTTTTTCTTATCTTCCTCACAAAGGt                                                                                  >  1:359815/1‑150 (MQ=255)
                                                                       aGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTa                                                                                <  1:898570/150‑1 (MQ=255)
                                                                        gTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAAACATTGATTCACTGAATGACCGCTTAGTCCGGCATTATCTTTTTATCAACTTCCTGCTTTTCCTGCCGAAATTTTTTCTTATCTACCTCACAAATGTTAg                                                                               >  1:800118/1‑150 (MQ=255)
                                                                         tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATGCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                              <  1:300542/150‑1 (MQ=255)
                                                                          aTTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTTCCTCACAAAGGTTAGCa                                                                             >  2:1222255/1‑150 (MQ=255)
                                                                          aTTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCa                                                                             >  1:512961/1‑150 (MQ=255)
                                                                          aTTCAACATCTTTTCTGGTGTAGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCa                                                                             <  2:130117/150‑1 (MQ=255)
                                                                             cAACATCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                          >  2:595635/1‑150 (MQ=255)
                                                                             cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                          >  1:1318837/1‑150 (MQ=255)
                                                                             cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                          >  1:1455596/1‑150 (MQ=255)
                                                                             cAACATCTTTTCTGGTGTTGGTAAAAAAGACAAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTAAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                          >  2:906994/1‑150 (MQ=255)
                                                                             aaaCATCTTTTCTGGTGTTTGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCACGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                          <  1:887384/149‑1 (MQ=255)
                                                                                 aTCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActg                                                                      <  1:90236/150‑1 (MQ=255)
                                                                                  tCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgc                                                                     <  2:1109108/150‑1 (MQ=255)
                                                                                    ttttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgctg                                                                   >  1:1767482/1‑150 (MQ=255)
                                                                                     tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                                  <  1:23943/150‑1 (MQ=255)
                                                                                     tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                                  <  1:574132/150‑1 (MQ=255)
                                                                                             gtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCATGAATGCCCGCTTTGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCAGGCCGATATTTTTTCTTATCTAGCTCCCAAAGGTTAGCAATAACTGCTGGGATAATTc                                                          >  2:1324255/1‑150 (MQ=255)
                                                                                                ggTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGa                                                       >  1:715101/1‑150 (MQ=255)
                                                                                                 gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCAAAAAGGTTAGCAATAACTTCTGGGAAAATTCCTAg                                                      >  2:150533/1‑150 (MQ=255)
                                                                                                   aaaaaaGACCAGCCCGGACAATATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTATTCTCAACTTCCAGCTTTTCCGGCCGATATTTTTTCTTATCTACCTCACCAAGGTTAGCAAAAACTGCTGGGAAAATTCCGAGtt                                                    >  1:250369/1‑150 (MQ=255)
                                                                                                     aaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAg                                                  <  1:1523707/150‑1 (MQ=255)
                                                                                                     aaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAg                                                  >  2:531815/1‑150 (MQ=255)
                                                                                                       aaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTc                                                <  1:1359095/150‑1 (MQ=255)
                                                                                                          aCCAGCACGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGtt                                             <  1:822646/150‑1 (MQ=255)
                                                                                                           ccAGCACGGACAAACTCATTGATTCCCTGAATGCCCGCTGAGTCGGGCATTTTCGTTTTCGCAAGTTGCTGCGGTTCCGGCCGATATGTTTGCTTATCTGCCGCTCATAGGTTAGCGATAGCTGCTGGGAATATTCCGAGTGAGTCGGta                                            >  2:177442/1‑150 (MQ=255)
                                                                                                             atcccGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCTATATTTTTTCTTATCTACCTCACAAAGGTTAGCAAACACTGCTGGGAAAATTCCGAGTTAGGCGTtata                                          <  2:1433065/148‑1 (MQ=255)
                                                                                                                   taCAAAATCATTGATTCCCTGAATGCCCGCTTAGTCTGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                                    <  1:244224/149‑1 (MQ=255)
                                                                                                                   gACAAAATCATTGATTCCCTGTATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                                    <  1:491748/150‑1 (MQ=255)
                                                                                                                     cAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGcc                                  >  2:1319711/1‑150 (MQ=255)
                                                                                                                       aaaTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTAGTTATATTGTCGCCTa                                >  1:778283/1‑150 (MQ=255)
                                                                                                                            aTTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTTCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTATTCGTTATATTGTCGCCTACATaa                           <  2:732167/150‑1 (MQ=255)
                                                                                                                               gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                        >  1:698045/1‑150 (MQ=255)
                                                                                                                               gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                        <  2:419552/150‑1 (MQ=255)
                                                                                                                                 ttCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTa                      <  1:596185/150‑1 (MQ=255)
                                                                                                                                 ttCCCTGAATGCCCGCATAGGCGGGCATTTTCTTTTTCTCAACTTCCTGCTATTCCTGCCGATATATTTTCTTATCTACCTCACAATGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTa                      <  1:109823/150‑1 (MQ=255)
                                                                                                                                   cccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa                    >  1:1545713/1‑150 (MQ=255)
                                                                                                                                    ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                   >  2:831472/1‑150 (MQ=255)
                                                                                                                                    ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGAAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTTTATTGTCGCCTACATAACGTTACAt                   >  1:1626747/1‑150 (MQ=255)
                                                                                                                                    ccTGAATGACCGCTTAGTCGGGCATTTTATTTTTCTCAAATTAATGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTGGTTATATTGTCGCCTACATAACGTTAGAt                   >  2:1146600/1‑150 (MQ=255)
                                                                                                                                     cTGAATGCCCGCTTAGTCGTGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAtt                  <  1:885771/150‑1 (MQ=255)
                                                                                                                                      tGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTa                 >  1:1649986/1‑150 (MQ=255)
                                                                                                                                         aTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg              >  1:174297/1‑150 (MQ=255)
                                                                                                                                           gCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg            <  1:1255829/150‑1 (MQ=255)
                                                                                                                                           gCCCGCTTAGTCCGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg            <  2:343096/150‑1 (MQ=255)
                                                                                                                                                cTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc       <  2:1722524/150‑1 (MQ=255)
                                                                                                                                                cTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc       <  1:1752112/150‑1 (MQ=255)
                                                                                                                                                  tAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCaa     >  1:1271806/1‑150 (MQ=255)
                                                                                                                                                  tAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCaa     <  2:959535/150‑1 (MQ=255)
                                                                                                                                                     tCGGGCATTTTCTTATTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATgg  <  1:1695466/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATGG  >  NC_000913/794794‑795092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: