Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 838,550 G→A 6.2% R220W (CGG→TGG)  ybiX ← Fe(II)‑dependent oxygenase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913838,5500GA 6.2% 337.8 / 6.5 113R220W (CGG→TGG) ybiXFe(II)‑dependent oxygenase superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (47/58);  new base A (3/4);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

ATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATG  >  NC_000913/838401‑838699
                                                                                                                                                     |                                                                                                                                                     
aTTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCg                                                                                                                                                       >  1:1794160/1‑150 (MQ=255)
 ttGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgc                                                                                                                                                      >  1:234650/1‑150 (MQ=255)
 ttGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgc                                                                                                                                                      >  2:644245/1‑150 (MQ=255)
     aCTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgcagca                                                                                                                                                  <  2:1822661/150‑1 (MQ=255)
        gTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTTCAGTTAAGTGTAGCTAATCAAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGGAGAt                                                                                                                                               >  1:367168/1‑150 (MQ=255)
        gTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGAt                                                                                                                                               >  1:1234135/1‑150 (MQ=255)
        gTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGAt                                                                                                                                               >  2:586168/1‑150 (MQ=255)
         ttGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGAtt                                                                                                                                              <  2:74915/150‑1 (MQ=255)
          tGATCTGTTCATTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTa                                                                                                                                             <  1:211038/150‑1 (MQ=255)
           gATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACAGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTAt                                                                                                                                            <  1:1396140/150‑1 (MQ=255)
            aTCTGTTCGTTGACGGCGGCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATg                                                                                                                                           <  2:1218345/150‑1 (MQ=255)
                gTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGCCCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATaa                                                                                                                                       >  2:1638596/1‑150 (MQ=255)
                gTTCGTTGACGGCGGCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGTACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATaa                                                                                                                                       <  2:753048/150‑1 (MQ=255)
                  tCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAg                                                                                                                                     <  2:1211780/150‑1 (MQ=255)
                   cGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGa                                                                                                                                    >  2:1247920/1‑150 (MQ=255)
                      tGACGGCGTCATCGTTAGCCCAAGCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCACAGCAGATTATGATAAAGATTa                                                                                                                                 >  1:888708/1‑150 (MQ=255)
                      tGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTa                                                                                                                                 <  1:781258/150‑1 (MQ=255)
                      tGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCACAGCAGATTATGATAAAGATTa                                                                                                                                 >  1:798692/1‑150 (MQ=255)
                       gACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTaa                                                                                                                                <  1:1492994/150‑1 (MQ=255)
                       gACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTaa                                                                                                                                <  1:1088417/150‑1 (MQ=255)
                        aCGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGCCCATTCCCGCAGCAGATTATGATAAAGATTAag                                                                                                                               >  2:883530/1‑150 (MQ=255)
                         cGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCACATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAagc                                                                                                                              <  1:559022/150‑1 (MQ=255)
                            cGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAagcagc                                                                                                                           <  1:789974/150‑1 (MQ=255)
                             gTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCg                                                                                                                          >  1:1452416/1‑150 (MQ=255)
                               cATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGAc                                                                                                                        >  1:424833/1‑150 (MQ=255)
                                 tCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAg                                                                                                                      <  1:970840/150‑1 (MQ=255)
                                     tAGCCCAACCGGATTCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAtc                                                                                                                  <  2:191308/150‑1 (MQ=255)
                                     tAGCCCAACCGGATGCCATATCGCCCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGCCCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGagc                                                                                                                  >  2:1856640/1‑148 (MQ=255)
                                     tAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAtc                                                                                                                  <  2:1779730/150‑1 (MQ=255)
                                     tAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAtc                                                                                                                  <  1:608677/150‑1 (MQ=255)
                                       gCCCAACCGGATGCCATATCGCCCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGCCCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAtctc                                                                                                                >  1:1203253/1‑150 (MQ=255)
                                           aaCCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAGCTCTTCa                                                                                                            <  1:1131374/150‑1 (MQ=255)
                                            aCCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCAc                                                                                                           <  1:1578225/150‑1 (MQ=255)
                                            aCCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGATCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCAc                                                                                                           <  1:104639/150‑1 (MQ=255)
                                               ggATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACttt                                                                                                        <  1:1491678/150‑1 (MQ=255)
                                                 aTGCCATATCGACCTCCCCATATCAAAACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCg                                                                                                      <  1:1256985/150‑1 (MQ=255)
                                                   gCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGcc                                                                                                    >  1:912684/1‑150 (MQ=255)
                                                   gCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGcc                                                                                                    <  1:1148888/150‑1 (MQ=255)
                                                     cATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCAAACCCGCACCAGATAATGATAAAGATTAACCCGCGACAGGCTCTCTTCACTTTCGCCGt                                                                                                  >  2:1628801/1‑150 (MQ=255)
                                                           gACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGCCCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGc                                                                                            >  1:1635675/1‑150 (MQ=255)
                                                             ccTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCtt                                                                                          >  1:1344737/1‑150 (MQ=255)
                                                              cTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCttt                                                                                         <  2:1269131/150‑1 (MQ=255)
                                                                ccccATATCAATACTTGTACAGTTAAGTGTAGCTAAGCCAGGGACGAACTCGGGCAGTTAAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTAGGATAAAGATTAAGCAGACACACGATCTCTTCACTTTCGCCGTACCGTCtttta                                                                                       >  2:533271/1‑149 (MQ=255)
                                                                 cccATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCa                                                                                      <  1:694360/150‑1 (MQ=255)
                                                                 cccATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGAACTCCGACCCTTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTcc                                                                                      >  2:7624/1‑149 (MQ=255)
                                                                 cccATATCAATACTTGTACAGTTAAGTGAAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCa                                                                                      <  1:383947/150‑1 (MQ=255)
                                                                  ccATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAg                                                                                     >  2:642247/1‑150 (MQ=255)
                                                                   cttatCAATACTTTTACAGTTCAGTGTAGATAATCCAGGTACTAACTCGGGAAGTTCAAGCATCAGAGCTCCGTCCATTCTCTCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGc                                                                                    <  1:272278/148‑1 (MQ=255)
                                                                    atatCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCg                                                                                   <  1:1219505/150‑1 (MQ=255)
                                                                      atCAATACTTGTACAGTTAAGTGTAGCTATTCCACGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCACAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGAc                                                                                 <  1:810988/150‑1 (MQ=255)
                                                                      atCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGAc                                                                                 <  2:858195/150‑1 (MQ=255)
                                                                      atCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGAc                                                                                 <  1:1122910/150‑1 (MQ=255)
                                                                       tCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCACAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACt                                                                                <  2:798692/150‑1 (MQ=255)
                                                                       tCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCACAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACt                                                                                <  2:888708/150‑1 (MQ=255)
                                                                               tGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCCACCATTCCCGCAGCAGATTATCATAAAGATTAAGCAGCGCCAGGTGCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAAAAttg                                                                        >  2:572196/1‑150 (MQ=255)
                                                                                gTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATAttgt                                                                       >  1:531313/1‑150 (MQ=255)
                                                                                gTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATAttgt                                                                       >  1:1841959/1‑150 (MQ=255)
                                                                                gTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATAttgt                                                                       >  2:1574191/1‑150 (MQ=255)
                                                                                gTACAGCTAAGTGTAGGTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTTAGCAGCTACATGAGCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATAttgt                                                                       <  1:305026/150‑1 (MQ=255)
                                                                                    aGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGAGCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTc                                                                   >  2:226098/1‑150 (MQ=255)
                                                                                     gTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATTAAGATTAAGCAGCGACAGGATCTCTGCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTcc                                                                  >  2:1175547/1‑150 (MQ=255)
                                                                                     gTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTcc                                                                  >  2:2825/1‑150 (MQ=255)
                                                                                     gTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTcc                                                                  <  2:710962/150‑1 (MQ=255)
                                                                                     gTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTcc                                                                  <  1:681505/150‑1 (MQ=255)
                                                                                      ttAAGTGTAGCTAATCCAGGGACGAACTCGTGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATAAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCa                                                                 <  1:295786/150‑1 (MQ=255)
                                                                                         aGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGtt                                                              <  1:644245/150‑1 (MQ=255)
                                                                                           tgtAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCa                                                            >  2:459877/1‑150 (MQ=255)
                                                                                              aGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCCCTATCGCCGTAGCGGCTTTTCAGCGCCTGAATATTGTTGTCCAGTTCAAAc                                                         >  2:268287/1‑150 (MQ=255)
                                                                                              aGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAAc                                                         >  2:1067030/1‑150 (MQ=255)
                                                                                              aGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAAc                                                         <  2:234650/150‑1 (MQ=255)
                                                                                                 tAATCCAGGGACGAACTCGTGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGc                                                      <  1:913067/150‑1 (MQ=255)
                                                                                                 tAATCCAGGGACGAACTCGGACAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGc                                                      <  2:607559/150‑1 (MQ=255)
                                                                                                      cAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGgc                                                 >  1:1443564/1‑150 (MQ=255)
                                                                                                      cAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCAGTAGCGGCTTTTCAGAGACTGAATATTGTTGACCAGTACAAACAGCATGgc                                                 >  1:693125/1‑150 (MQ=255)
                                                                                                       aGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGgcg                                                >  2:1287176/1‑150 (MQ=255)
                                                                                                        gggACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGgcgc                                               >  2:1861311/1‑150 (MQ=255)
                                                                                                        gggACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGgcgc                                               >  2:494300/1‑150 (MQ=255)
                                                                                                          gaagAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGgcgcgc                                             <  1:1621857/147‑1 (MQ=255)
                                                                                                            cGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCCAACAGCATGGCGCGCtt                                           >  2:17305/1‑150 (MQ=255)
                                                                                                               aCTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCttttt                                        <  1:1368955/150‑1 (MQ=255)
                                                                                                                cgcGGGCAGTTCAACCATGCTCCCTCCGCCCAGTCCCGCAGCAGAATATGATAAAGATTACGCAGCGACCGGAGCTCATCACTTTCGCCGTAGCGGCTTTTCAGCGACTTAATATTGTAGTCCAGTTCAAACAGCATGGCGCGCTTTTTa                                       <  2:1117304/148‑1 (MQ=255)
                                                                                                                cTCGTGCAGTTCAAGCATCAGATCTCCGAACAATCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGCATCTCTTCACTATCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGATCAAACAGCATGGCGCGCTTTTTa                                       <  1:380212/150‑1 (MQ=255)
                                                                                                                cTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGTGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTa                                       >  1:1702129/1‑150 (MQ=255)
                                                                                                                         ttCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCgg                              <  1:1814215/150‑1 (MQ=255)
                                                                                                                           cAAGCATCAGCTCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGat                            >  2:468085/1‑150 (MQ=255)
                                                                                                                           cAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGat                            <  2:1386695/150‑1 (MQ=255)
                                                                                                                           cAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTATTCACTTTCGCCGTAGCGGCTTTTCAGCGCCTGAATATTGTTGTCCAGTTCAAACCGCATGGCGCGCTTTTTATCATCGAGGat                            >  1:1641018/1‑150 (MQ=255)
                                                                                                                            aaGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGatc                           <  2:1452416/150‑1 (MQ=255)
                                                                                                                              gCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTACAGTTCAAACAGCATGGCGCGCTTTTTATAATCGCGGatcaa                         >  2:816950/1‑149 (MQ=255)
                                                                                                                                   aGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACt                    <  1:1638596/150‑1 (MQ=255)
                                                                                                                                    gATCTACGACCATTCCAGCAGCATATTATGATATAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGAGGCTTTTCAGCGAATGAATATTGTTGTCCAGTTCAATCAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTg                   <  2:774336/150‑1 (MQ=255)
                                                                                                                                     aTCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTgg                  <  1:723500/150‑1 (MQ=255)
                                                                                                                                         ccGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATcc              >  2:51793/1‑150 (MQ=255)
                                                                                                                                          cGACCATTCCCGCAGCAGATTATGATAAATATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCca             <  2:912684/150‑1 (MQ=255)
                                                                                                                                           gACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCcac            <  2:551191/150‑1 (MQ=255)
                                                                                                                                                ttCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATaaa       <  1:1566610/150‑1 (MQ=255)
                                                                                                                                                ttCCCGCAGCAGATTATGAAAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATaaa       <  1:931543/150‑1 (MQ=255)
                                                                                                                                                  cccGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAatg     >  2:339697/1‑150 (MQ=255)
                                                                                                                                                  cccGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATATCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGAATGGATACACATAAatg     >  1:173809/1‑150 (MQ=255)
                                                                                                                                                     gcagcaGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAatgatg  <  1:1088497/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACTGAATATTGTTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATG  >  NC_000913/838401‑838699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: