Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 854,790 T→C 5.6% intergenic (+25/+34) ybiR → / ← ldtB putative transporter/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913854,7900TC 5.6% 429.2 / 10.7 142intergenic (+25/+34)ybiR/ldtBputative transporter/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (63/71);  new base C (5/3);  total (68/74)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 143 total aligned reads displayed.

TGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGC  >  NC_000913/854641‑854938
                                                                                                                                                     |                                                                                                                                                    
tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAt                                                                                                                                                      <  2:1491426/150‑1 (MQ=255)
   ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTAt                                                                                                                                                   >  2:1536614/1‑150 (MQ=255)
    tGCGCTACGTTTGGCGAACGATGGCCGCATCTGGTGGCGTTTCCTTCTCTATTCAATTCCGATGCAGTTGTGGGCGGCGTTGGTGGGATATGTGTTGTTAGTTATTCTCCCGTCGAACTAGTGTCTGGCAAATAAAAATGGCGGATTATg                                                                                                                                                  <  2:1598757/150‑1 (MQ=255)
    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTTGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                                                                                                                  <  2:934357/150‑1 (MQ=255)
        cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTc                                                                                                                                              <  1:1750083/150‑1 (MQ=255)
         tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGtac                                                                                                                                             >  2:1091589/1‑148 (MQ=255)
          aCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCg                                                                                                                                            >  1:757135/1‑150 (MQ=255)
              attgctaaCGATCGCCTCATCTGGTGGCGTTACCATCTCTATTCAATAACGATGCTGTTGTGGGCGGCGTTGGTGGGACATGTTTTGTTAGTTATACTCGCGGCCAACTAGGCTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                                                                                                        <  1:148957/144‑1 (MQ=255)
               gggCGAACGATCGCCTAATCTGCTGGCGTTTCCATCACTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCttt                                                                                                                                       <  2:149044/149‑1 (MQ=255)
                ggCGAACGATCGCCGCATCTGGTGGCGATTCCATCTCTATTCAATACCGATGGTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCACGGCCAAATAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCtttt                                                                                                                                      >  2:1358489/1‑150 (MQ=255)
                 gCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttt                                                                                                                                     <  1:1189674/150‑2 (MQ=255)
                  cGAACGATCGCCGCGTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                    >  1:1327535/1‑150 (MQ=255)
                  cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                    >  2:514286/1‑150 (MQ=255)
                  cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                    >  1:1607940/1‑150 (MQ=255)
                  cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCATTTGc                                                                                                                                    >  2:752698/1‑150 (MQ=255)
                   gAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                                                                                                                   >  1:138514/1‑150 (MQ=255)
                      cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                                                                                                                >  1:753261/1‑150 (MQ=255)
                      cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttattt                                                                                                                                <  2:777844/150‑7 (MQ=255)
                       gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGCAGCTGTCGTGGGCGGCGTTGGTGGGATATGGTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttatttg                                                                                                                               <  2:308250/150‑8 (MQ=255)
                       gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTaa                                                                                                                               >  1:1145849/1‑150 (MQ=255)
                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtt                                                                                                                            >  2:1060510/1‑150 (MQ=255)
                           gccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGttt                                                                                                                           >  1:563036/1‑150 (MQ=255)
                             cgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                                                         <  1:666948/150‑1 (MQ=255)
                             cgcATCTGGTGGCGTTTCCATATCTATTAAATACCTATGCTGTTGTGGGGGGCGTTGGTGGGATATTTTTTGTTAGTTATACTCCCGGACAAGTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                                                         <  2:37694/150‑1 (MQ=255)
                              gcATCTGGTGGCGGTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGa                                                                                                                        <  1:1557125/150‑1 (MQ=255)
                               cATCTGGGGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTGGGGGGGATATGTTTTGTTAGTTATACTGCCGGCCAACTAGGGTCTGGCATATAAAAATGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                                                                                                                       <  2:1766766/150‑1 (MQ=255)
                                aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                      >  1:132407/1‑150 (MQ=255)
                                  cTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGGGGGATATGTTTTGTTAGGTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                                                                    <  1:378378/150‑1 (MQ=255)
                                   tttgggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGTCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                   <  1:401170/146‑1 (MQ=255)
                                      tggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGAGGATTATGAGTCCGACTTTTGCTTTAAGTTTTGAAGATTTAt                                                                                                                >  2:936980/1‑150 (MQ=255)
                                       ggCTTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGAGGCGTTGGTGGGATATGTATTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCATTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                               >  1:1047474/1‑150 (MQ=255)
                                       ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                               <  2:80816/150‑1 (MQ=255)
                                          ggttCCATCTCTATTCAATACCGATGCTGATGTGGGCGTCGTTGGTGGGATATGTTTTGTTAGTTTTACTCACGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                                                            <  1:1021329/148‑1 (MQ=255)
                                            ttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                                                          <  1:1372713/150‑1 (MQ=255)
                                            ttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                                                          >  1:288741/1‑150 (MQ=255)
                                              ccATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                                                        >  2:429272/1‑150 (MQ=255)
                                                 tctcTATTCACAACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                                                                     <  2:1599077/150‑1 (MQ=255)
                                                  ctctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTGTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                                                                    >  2:103479/1‑150 (MQ=255)
                                                   tctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCgg                                                                                                   >  2:532100/1‑150 (MQ=255)
                                                    ctATTCAATCCCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                  <  1:1860868/150‑1 (MQ=255)
                                                    ctATTCAATACCGCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTCAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                  <  1:1007127/150‑1 (MQ=255)
                                                        tCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGTCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc                                                                                              >  1:202081/1‑150 (MQ=255)
                                                          aaTACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg                                                                                            >  1:1592670/1‑150 (MQ=255)
                                                               cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                                                       >  2:1589679/1‑150 (MQ=255)
                                                                gATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCgg                                                                                      >  2:66464/1‑150 (MQ=255)
                                                                   gCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttt                                                                                   >  2:1754696/1‑150 (MQ=255)
                                                                    cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                  >  1:1812399/1‑150 (MQ=255)
                                                                     tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                 <  2:1620541/150‑1 (MQ=255)
                                                                     tgttgtGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCACCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                 <  1:889134/150‑1 (MQ=255)
                                                                     gtgtgtgGGCGGCGTGGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                 <  2:1029088/147‑1 (MQ=255)
                                                                       ttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCAGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                                                               <  1:1218754/150‑1 (MQ=255)
                                                                        tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                              >  1:999666/1‑150 (MQ=255)
                                                                        tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGATATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                              <  1:426206/150‑1 (MQ=255)
                                                                         gtgGGCGGCGTTGGTGGGATATGTTTGGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATc                                                                             <  2:1297336/150‑1 (MQ=255)
                                                                          tgGGCGGCGTTGTTTGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTTCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCg                                                                            <  1:844472/150‑1 (MQ=255)
                                                                                 cgTTGGTGGTATATGTGTTTTTTGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGTTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCttcttc                                                                     <  1:892782/150‑1 (MQ=255)
                                                                                    tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                  <  1:1782062/150‑1 (MQ=255)
                                                                                    tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                  <  2:816916/150‑1 (MQ=255)
                                                                                     ggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGa                                                                 <  2:1309240/150‑1 (MQ=255)
                                                                                      gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                                                                >  2:602189/1‑150 (MQ=255)
                                                                                        gggATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCATCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaaca                                                              >  1:857866/1‑150 (MQ=255)
                                                                                          gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                                                            >  2:550264/1‑150 (MQ=255)
                                                                                           atatGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                                                           <  1:725029/150‑1 (MQ=255)
                                                                                               gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                       >  1:514362/1‑150 (MQ=255)
                                                                                               gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                       >  2:1780984/1‑150 (MQ=255)
                                                                                                gtttGTTAGTTATACTCCCGGCCAAGTAGGGTCTGGCAAATAAATAAGTCTGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAACGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTc                                                      <  2:1047474/149‑1 (MQ=255)
                                                                                                 tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                     <  2:798934/150‑1 (MQ=255)
                                                                                                      tagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATc                                                >  1:1455669/1‑150 (MQ=255)
                                                                                                        gttaTACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGGCAACATCTg                                              <  2:258768/150‑1 (MQ=255)
                                                                                                          tataCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGc                                            >  1:830452/1‑150 (MQ=255)
                                                                                                             aCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTTTGGTTTAAGTTTTGAAGATTAATACAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTgc                                         >  2:545391/1‑149 (MQ=255)
                                                                                                              cTCCCGGCCAACTAGTGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGAAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                        <  1:364641/150‑1 (MQ=255)
                                                                                                              cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                        >  1:677514/1‑150 (MQ=255)
                                                                                                              cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                        >  2:1670509/1‑150 (MQ=255)
                                                                                                              cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                        >  2:50181/1‑150 (MQ=255)
                                                                                                                cccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCg                                      >  1:1126283/1‑150 (MQ=255)
                                                                                                                 ccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCgg                                     <  1:803502/150‑1 (MQ=255)
                                                                                                                  cGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGt                                    >  2:579591/1‑150 (MQ=255)
                                                                                                                     ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                 <  1:558787/150‑1 (MQ=255)
                                                                                                                     ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                 <  2:750155/150‑1 (MQ=255)
                                                                                                                         atAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGATTTAAGTTTTGAAGATTAATTCAGACGAACCTGCATCACGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTc                             <  1:275655/149‑1 (MQ=255)
                                                                                                                           aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                           <  2:1592670/150‑1 (MQ=255)
                                                                                                                              gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCaa                        >  2:1412352/1‑149 (MQ=255)
                                                                                                                                   gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGTAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGctct                   <  2:288741/150‑1 (MQ=255)
                                                                                                                                   gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                     <  2:1214300/148‑1 (MQ=255)
                                                                                                                                   gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                     >  1:1214300/1‑148 (MQ=255)
                                                                                                                                        tAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATGGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGTCCGGTCACTGTCTGCACGCTCTTCGTc              >  1:1667685/1‑150 (MQ=255)
                                                                                                                                         gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa             >  1:811053/3‑150 (MQ=255)
                                                                                                                                         gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa             >  2:1640533/3‑150 (MQ=255)
                                                                                                                                         aaaaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCCCGCTCTTCGTCa             >  1:189111/1‑150 (MQ=255)
                                                                                                                                           aaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATAAATTCAGACGAAGCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACTACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg           <  1:103479/150‑1 (MQ=255)
                                                                                                                                           aaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACACCCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg           >  1:1077171/1‑150 (MQ=255)
                                                                                                                                            aaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGCCGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg          >  2:474444/1‑150 (MQ=255)
                                                                                                                                            aaaCGCGCATTATGAGTCCGCCTTTTGCTTTAAGTTTAGAAGATTAAATCCGCCGACCCGGCATCCCGGAGCGGTTTTTAATCGCATCATCAAGCACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg          <  2:127736/150‑1 (MQ=255)
                                                                                                                                             aaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGTCGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGATCAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgtgt         <  2:672263/150‑1 (MQ=255)
                                                                                                                                               ggCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTAAGACGAACCGGCATCCCGGAGCGGTATTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGTTCACTGTCTGCACGCTCTTCGTAAGGGTaa       >  2:1013831/1‑150 (MQ=255)
                                                                                                                                                 cGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAAtt     >  2:1650738/1‑150 (MQ=255)
                                                                                                                                                    aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc  <  2:753261/150‑1 (MQ=255)
                                                                                                                                                    aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc  <  2:202081/150‑1 (MQ=255)
                                                                                                                                                    aTTAGGAGTGCGCCTTTTGCTTTAAGTTTTGATGATTATTTCAGACGAACGGGCTTCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc  <  2:1190671/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGC  >  NC_000913/854641‑854938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: