Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 854,792 A→C 5.6% intergenic (+27/+32) ybiR → / ← ldtB putative transporter/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913854,7920AC 5.6% 407.5 / 10.9 143intergenic (+27/+32)ybiR/ldtBputative transporter/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base A (63/72);  new base C (5/3);  total (68/75)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

Only 100 of 145 total aligned reads displayed.

TTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACA  >  NC_000913/854644‑854941
                                                                                                                                                    |                                                                                                                                                     
 tGCGCTACGTTTGGCGAACGATGGCCGCATCTGGTGGCGTTTCCTTCTCTATTCAATTCCGATGCAGTTGTGGGCGGCGTTGGTGGGATATGTGTTGTTAGTTATTCTCCCGTCGAACTAGTGTCTGGCAAATAAAAATGGCGGATTATg                                                                                                                                                     <  2:1598757/150‑1 (MQ=255)
 tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTTGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                                                                                                                     <  2:934357/150‑1 (MQ=255)
  gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                                                                                                                    >  1:1248424/1‑150 (MQ=255)
      tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGtac                                                                                                                                                >  2:1091589/1‑148 (MQ=255)
       aCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCg                                                                                                                                               >  1:757135/1‑150 (MQ=255)
           attgctaaCGATCGCCTCATCTGGTGGCGTTACCATCTCTATTCAATAACGATGCTGTTGTGGGCGGCGTTGGTGGGACATGTTTTGTTAGTTATACTCGCGGCCAACTAGGCTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                                                                                                           <  1:148957/144‑1 (MQ=255)
            gggCGAACGATCGCCTAATCTGCTGGCGTTTCCATCACTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCttt                                                                                                                                          <  2:149044/149‑1 (MQ=255)
             ggCGAACGATCGCCGCATCTGGTGGCGATTCCATCTCTATTCAATACCGATGGTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCACGGCCAAATAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCtttt                                                                                                                                         >  2:1358489/1‑150 (MQ=255)
              gCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttt                                                                                                                                        <  1:1189674/150‑2 (MQ=255)
               cGAACGATCGCCGCGTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                       >  1:1327535/1‑150 (MQ=255)
               cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                       >  2:514286/1‑150 (MQ=255)
               cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCATTTGc                                                                                                                                       >  2:752698/1‑150 (MQ=255)
                gAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                                                                                                                      >  1:138514/1‑150 (MQ=255)
                   cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                                                                                                                   >  1:753261/1‑150 (MQ=255)
                   cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttattt                                                                                                                                   <  2:777844/150‑7 (MQ=255)
                    gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGCAGCTGTCGTGGGCGGCGTTGGTGGGATATGGTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttatttg                                                                                                                                  <  2:308250/150‑8 (MQ=255)
                    gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTaa                                                                                                                                  >  1:1145849/1‑150 (MQ=255)
                      tCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGt                                                                                                                                <  1:1563176/150‑1 (MQ=255)
                       cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtt                                                                                                                               >  2:1060510/1‑150 (MQ=255)
                            cATCTGGGGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTGGGGGGGATATGTTTTGTTAGTTATACTGCCGGCCAACTAGGGTCTGGCATATAAAAATGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                                                                                                                          <  2:1766766/150‑1 (MQ=255)
                             aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                         >  1:132407/1‑150 (MQ=255)
                             aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                         <  1:1648808/150‑1 (MQ=255)
                             aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                         <  1:1766196/150‑1 (MQ=255)
                                tttgggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGTCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                      <  1:401170/146‑1 (MQ=255)
                                tgggggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                      <  1:408074/150‑1 (MQ=255)
                                   tggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGAGGATTATGAGTCCGACTTTTGCTTTAAGTTTTGAAGATTTAt                                                                                                                   >  2:936980/1‑150 (MQ=255)
                                    ggCTTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGAGGCGTTGGTGGGATATGTATTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCATTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                  >  1:1047474/1‑150 (MQ=255)
                                    ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                  <  2:80816/150‑1 (MQ=255)
                                       ggttCCATCTCTATTCAATACCGATGCTGATGTGGGCGTCGTTGGTGGGATATGTTTTGTTAGTTTTACTCACGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                                                               <  1:1021329/148‑1 (MQ=255)
                                         ttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                                                             <  1:1372713/150‑1 (MQ=255)
                                          tCCATCTCTATTCAATACCGATGCTGTTTTGGGCGTCGTTTGTGGGATATGTTTTGTTAGTTATACTCCCTGCCAAATAGTGTCTGGCAAATAAAAAAGGCTGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGAAGATTAATTCATACg                                                                                                            <  2:1429291/150‑1 (MQ=255)
                                           ccATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                                                           >  2:429272/1‑150 (MQ=255)
                                              tctcTATTCACAACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                                                                        <  2:1599077/150‑1 (MQ=255)
                                               ctctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTGTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                                                                       >  2:103479/1‑150 (MQ=255)
                                                tctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCgg                                                                                                      >  2:532100/1‑150 (MQ=255)
                                                 ctATTCAATACCGCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTCAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                     <  1:1007127/150‑1 (MQ=255)
                                                    ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                                                                  >  2:558787/1‑150 (MQ=255)
                                                       aaTACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg                                                                                               >  1:1592670/1‑150 (MQ=255)
                                                            cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                                                          >  2:1589679/1‑150 (MQ=255)
                                                             gATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCgg                                                                                         >  2:66464/1‑150 (MQ=255)
                                                                gCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttt                                                                                      >  2:1754696/1‑150 (MQ=255)
                                                                 cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                     >  1:1812399/1‑150 (MQ=255)
                                                                 cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                     <  1:216820/150‑1 (MQ=255)
                                                                  tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                    <  2:1620541/150‑1 (MQ=255)
                                                                  tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGGCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                    <  1:632281/150‑1 (MQ=255)
                                                                  tgttgtGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCACCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                    <  1:889134/150‑1 (MQ=255)
                                                                  gtgtgtgGGCGGCGTGGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                    <  2:1029088/147‑1 (MQ=255)
                                                                     tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                                 >  1:999666/1‑150 (MQ=255)
                                                                     tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGATATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                                 <  1:426206/150‑1 (MQ=255)
                                                                      gtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATc                                                                                <  1:526569/150‑1 (MQ=255)
                                                                      gtgGGCGGCGTTGGTGGGATATGTTTGGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATc                                                                                <  2:1297336/150‑1 (MQ=255)
                                                                       tgGGCGGCGTTGTTTGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTTCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCg                                                                               <  1:844472/150‑1 (MQ=255)
                                                                              cgTTGGTGGTATATGTGTTTTTTGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGTTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCttcttc                                                                        <  1:892782/150‑1 (MQ=255)
                                                                                 tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                     <  2:816916/150‑1 (MQ=255)
                                                                                 tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                     <  1:1782062/150‑1 (MQ=255)
                                                                                   gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                                                                   >  2:602189/1‑150 (MQ=255)
                                                                                     gggATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCATCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaaca                                                                 >  1:857866/1‑150 (MQ=255)
                                                                                       gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                                                               >  2:550264/1‑150 (MQ=255)
                                                                                        atatGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                                                              <  1:725029/150‑1 (MQ=255)
                                                                                            gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                          >  2:1780984/1‑150 (MQ=255)
                                                                                             gtttGTTAGTTATACTCCCGGCCAAGTAGGGTCTGGCAAATAAATAAGTCTGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAACGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTc                                                         <  2:1047474/149‑1 (MQ=255)
                                                                                              tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                        <  2:493460/150‑1 (MQ=255)
                                                                                              tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                        <  2:798934/150‑1 (MQ=255)
                                                                                                   tagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATc                                                   >  1:1455669/1‑150 (MQ=255)
                                                                                                     gttaTACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGGCAACATCTg                                                 <  2:258768/150‑1 (MQ=255)
                                                                                                     gttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCCTCCAGGAGCGGTTTTTAATCTCTTCATCAAGAACAACCTGGTCAACATCTg                                                 >  2:149814/1‑150 (MQ=255)
                                                                                                          aCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTTTGGTTTAAGTTTTGAAGATTAATACAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTgc                                            >  2:545391/1‑149 (MQ=255)
                                                                                                           cTCCCGGCCAACTAGTGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGAAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                           <  1:364641/150‑1 (MQ=255)
                                                                                                           cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                           >  2:50181/1‑150 (MQ=255)
                                                                                                           cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                           >  2:1670509/1‑150 (MQ=255)
                                                                                                           cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                           >  1:677514/1‑150 (MQ=255)
                                                                                                             cccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCg                                         >  1:1126283/1‑150 (MQ=255)
                                                                                                              ccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCgg                                        <  1:803502/150‑1 (MQ=255)
                                                                                                                  ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                    <  1:558787/150‑1 (MQ=255)
                                                                                                                    aaCTAGGGTCTGGCAAATAAAAACGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTg                                  <  2:1353060/150‑1 (MQ=255)
                                                                                                                      atAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGATTTAAGTTTTGAAGATTAATTCAGACGAACCTGCATCACGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTc                                <  1:275655/149‑1 (MQ=255)
                                                                                                                        aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                              <  2:1592670/150‑1 (MQ=255)
                                                                                                                           gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCAc                           <  2:419046/150‑1 (MQ=255)
                                                                                                                               ggCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTTGCTGACCGGTCACTGTCTGCACGcta                       >  2:696790/1‑149 (MQ=255)
                                                                                                                                gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGTAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGctct                      <  2:288741/150‑1 (MQ=255)
                                                                                                                                gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                        >  1:1214300/1‑148 (MQ=255)
                                                                                                                                gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                        <  2:1214300/148‑1 (MQ=255)
                                                                                                                                  aaaTAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTc                    >  1:1870238/1‑150 (MQ=255)
                                                                                                                                     tAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATGGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGTCCGGTCACTGTCTGCACGCTCTTCGTc                 >  1:1667685/1‑150 (MQ=255)
                                                                                                                                      gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                >  2:1640533/3‑150 (MQ=255)
                                                                                                                                      gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                >  1:811053/3‑150 (MQ=255)
                                                                                                                                      gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                >  1:811059/3‑150 (MQ=255)
                                                                                                                                      aaaaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCCCGCTCTTCGTCa                >  1:189111/1‑150 (MQ=255)
                                                                                                                                        aaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATAAATTCAGACGAAGCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACTACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg              <  1:103479/150‑1 (MQ=255)
                                                                                                                                        aaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACACCCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg              >  1:1077171/1‑150 (MQ=255)
                                                                                                                                         aaaCGCGCATTATGAGTCCGCCTTTTGCTTTAAGTTTAGAAGATTAAATCCGCCGACCCGGCATCCCGGAGCGGTTTTTAATCGCATCATCAAGCACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg             <  2:127736/150‑1 (MQ=255)
                                                                                                                                          aaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGTCGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGATCAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgtgt            <  2:672263/150‑1 (MQ=255)
                                                                                                                                            ggCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTAAGACGAACCGGCATCCCGGAGCGGTATTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGTTCACTGTCTGCACGCTCTTCGTAAGGGTaa          >  2:1013831/1‑150 (MQ=255)
                                                                                                                                             gCGGATTATGAGTCCGCCTTTTGCTTTATGTTTTGAATATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAAAAACCTGGTCAACATCTGGCTGACCTGTCACTGTCTGCACGCTCTTCGTAAGGGTAAt         >  1:352180/1‑150 (MQ=255)
                                                                                                                                              cGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAAtt        >  2:1650738/1‑150 (MQ=255)
                                                                                                                                                 aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc     <  2:753261/150‑1 (MQ=255)
                                                                                                                                                 aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc     <  2:202081/150‑1 (MQ=255)
                                                                                                                                                 aTTAGGAGTGCGCCTTTTGCTTTAAGTTTTGATGATTATTTCAGACGAACGGGCTTCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc     <  2:1190671/150‑1 (MQ=255)
                                                                                                                                                   tATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGcac   <  1:602189/150‑1 (MQ=255)
                                                                                                                                                    aTGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGCCCGGGCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGcaca  >  2:616991/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACA  >  NC_000913/854644‑854941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: