Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 854,794 G→T 5.7% intergenic (+29/+30) ybiR → / ← ldtB putative transporter/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913854,7940GT 5.7% 400.5 / 12.3 142intergenic (+29/+30)ybiR/ldtBputative transporter/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base G (64/70);  new base T (5/3);  total (69/73)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 145 total aligned reads displayed.

TGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAA  >  NC_000913/854645‑854942
                                                                                                                                                     |                                                                                                                                                    
tGCGCTACGTTTGGCGAACGATGGCCGCATCTGGTGGCGTTTCCTTCTCTATTCAATTCCGATGCAGTTGTGGGCGGCGTTGGTGGGATATGTGTTGTTAGTTATTCTCCCGTCGAACTAGTGTCTGGCAAATAAAAATGGCGGATTATg                                                                                                                                                      <  2:1598757/150‑1 (MQ=255)
tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTTGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                                                                                                                      <  2:934357/150‑1 (MQ=255)
 gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                                                                                                                     >  1:1248424/1‑150 (MQ=255)
      aCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCg                                                                                                                                                >  1:757135/1‑150 (MQ=255)
          attgctaaCGATCGCCTCATCTGGTGGCGTTACCATCTCTATTCAATAACGATGCTGTTGTGGGCGGCGTTGGTGGGACATGTTTTGTTAGTTATACTCGCGGCCAACTAGGCTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                                                                                                            <  1:148957/144‑1 (MQ=255)
           gggCGAACGATCGCCTAATCTGCTGGCGTTTCCATCACTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCttt                                                                                                                                           <  2:149044/149‑1 (MQ=255)
             gCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttt                                                                                                                                         <  1:1189674/150‑2 (MQ=255)
              cGAACGATCGCCGCGTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                        >  1:1327535/1‑150 (MQ=255)
              cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                        >  2:514286/1‑150 (MQ=255)
              cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCATTTGc                                                                                                                                        >  2:752698/1‑150 (MQ=255)
               gAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                                                                                                                       >  1:138514/1‑150 (MQ=255)
                  cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                                                                                                                    >  1:753261/1‑150 (MQ=255)
                  cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttattt                                                                                                                                    <  2:777844/150‑7 (MQ=255)
                   gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGCAGCTGTCGTGGGCGGCGTTGGTGGGATATGGTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttatttg                                                                                                                                   <  2:308250/150‑8 (MQ=255)
                   gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTaa                                                                                                                                   >  1:1145849/1‑150 (MQ=255)
                     tCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGt                                                                                                                                 <  1:1563176/150‑1 (MQ=255)
                      cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtt                                                                                                                                >  2:1060510/1‑150 (MQ=255)
                           cATCTGGGGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTGGGGGGGATATGTTTTGTTAGTTATACTGCCGGCCAACTAGGGTCTGGCATATAAAAATGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                                                                                                                           <  2:1766766/150‑1 (MQ=255)
                            aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                          >  1:132407/1‑150 (MQ=255)
                            aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                          <  1:1648808/150‑1 (MQ=255)
                            aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                          <  1:1766196/150‑1 (MQ=255)
                               tttgggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGTCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                       <  1:401170/146‑1 (MQ=255)
                               tggtggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                       <  2:1248424/150‑1 (MQ=255)
                               tgggggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                       <  1:408074/150‑1 (MQ=255)
                                  tggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGAGGATTATGAGTCCGACTTTTGCTTTAAGTTTTGAAGATTTAt                                                                                                                    >  2:936980/1‑150 (MQ=255)
                                   ggCTTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGAGGCGTTGGTGGGATATGTATTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCATTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                   >  1:1047474/1‑150 (MQ=255)
                                   ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                   <  2:80816/150‑1 (MQ=255)
                                      ggttCCATCTCTATTCAATACCGATGCTGATGTGGGCGTCGTTGGTGGGATATGTTTTGTTAGTTTTACTCACGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                                                                <  1:1021329/148‑1 (MQ=255)
                                        ttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                                                              <  1:1372713/150‑1 (MQ=255)
                                         tCCATCTCTATTCAATACCGATGCTGTTTTGGGCGTCGTTTGTGGGATATGTTTTGTTAGTTATACTCCCTGCCAAATAGTGTCTGGCAAATAAAAAAGGCTGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGAAGATTAATTCATACg                                                                                                             <  2:1429291/150‑1 (MQ=255)
                                          ccATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                                                            >  2:429272/1‑150 (MQ=255)
                                             tctcTATTCACAACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                                                                         <  2:1599077/150‑1 (MQ=255)
                                              ctctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTGTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                                                                        >  2:103479/1‑150 (MQ=255)
                                               tctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCgg                                                                                                       >  2:532100/1‑150 (MQ=255)
                                                ctATTCAATACCGCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTCAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                      <  1:1007127/150‑1 (MQ=255)
                                                   ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                                                                   >  2:558787/1‑150 (MQ=255)
                                                      aaTACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg                                                                                                >  1:1592670/1‑150 (MQ=255)
                                                           cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                                                           >  2:1589679/1‑150 (MQ=255)
                                                            gATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCgg                                                                                          >  2:66464/1‑150 (MQ=255)
                                                               gCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttt                                                                                       >  2:1754696/1‑150 (MQ=255)
                                                                cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                      >  1:1812399/1‑150 (MQ=255)
                                                                cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                      <  1:216820/150‑1 (MQ=255)
                                                                 tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                     <  2:1620541/150‑1 (MQ=255)
                                                                 tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGGCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                     <  1:632281/150‑1 (MQ=255)
                                                                 tgttgtGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCACCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                     <  1:889134/150‑1 (MQ=255)
                                                                 gtgtgtgGGCGGCGTGGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                     <  2:1029088/147‑1 (MQ=255)
                                                                    tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                                  >  1:999666/1‑150 (MQ=255)
                                                                    tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGATATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                                  <  1:426206/150‑1 (MQ=255)
                                                                     gtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATc                                                                                 <  1:526569/150‑1 (MQ=255)
                                                                      tgGGCGGCGTTGTTTGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTTCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCg                                                                                <  1:844472/150‑1 (MQ=255)
                                                                             cgTTGGTGGTATATGTGTTTTTTGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGTTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCttcttc                                                                         <  1:892782/150‑1 (MQ=255)
                                                                                tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                      <  1:1782062/150‑1 (MQ=255)
                                                                                tggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAg                                                                      <  2:816916/150‑1 (MQ=255)
                                                                                 ggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGa                                                                     <  2:1309240/150‑1 (MQ=255)
                                                                                  gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                                                                    >  2:602189/1‑150 (MQ=255)
                                                                                    gggATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCATCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaaca                                                                  >  1:857866/1‑150 (MQ=255)
                                                                                      gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                                                                >  2:550264/1‑150 (MQ=255)
                                                                                      gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTATGCTTAAAGTTTTGAAGATTAATTCAGACGAACCGTCATCCCGGAGCGGATTAAAATCGCATCATCAAGaacaac                                                                >  2:1116009/1‑150 (MQ=255)
                                                                                       atatGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                                                               <  1:725029/150‑1 (MQ=255)
                                                                                           gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                           >  2:1780984/1‑150 (MQ=255)
                                                                                             tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                         <  2:798934/150‑1 (MQ=255)
                                                                                             tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                         <  2:493460/150‑1 (MQ=255)
                                                                                                gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCTGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACa                                                      >  2:1057792/1‑150 (MQ=255)
                                                                                                  tagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATc                                                    >  1:1455669/1‑150 (MQ=255)
                                                                                                    gttaTACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGGCAACATCTg                                                  <  2:258768/150‑1 (MQ=255)
                                                                                                       ataCTCCCGGCCAACTAGGGCCTGGCAAATAAAAAAGCCGGATTATGAGTCTGCATTTTGCTTTAACTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCt                                               <  2:102345/150‑1 (MQ=255)
                                                                                                         aCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTTTGGTTTAAGTTTTGAAGATTAATACAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTgc                                             >  2:545391/1‑149 (MQ=255)
                                                                                                          cTCCCGGCCAACTAGTGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGAAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                            <  1:364641/150‑1 (MQ=255)
                                                                                                          cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                            >  2:50181/1‑150 (MQ=255)
                                                                                                          cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                            >  2:1670509/1‑150 (MQ=255)
                                                                                                          cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                            >  1:677514/1‑150 (MQ=255)
                                                                                                            cccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCg                                          >  1:1126283/1‑150 (MQ=255)
                                                                                                             ccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCgg                                         <  1:803502/150‑1 (MQ=255)
                                                                                                                 ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                     <  1:558787/150‑1 (MQ=255)
                                                                                                                   aaCTAGGGTCTGGCAAATAAAAACGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTg                                   <  2:1353060/150‑1 (MQ=255)
                                                                                                                     atAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGATTTAAGTTTTGAAGATTAATTCAGACGAACCTGCATCACGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTc                                 <  1:275655/149‑1 (MQ=255)
                                                                                                                       aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                               <  2:1592670/150‑1 (MQ=255)
                                                                                                                       aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                               <  1:964581/150‑1 (MQ=255)
                                                                                                                          gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCAc                            <  2:419046/150‑1 (MQ=255)
                                                                                                                          gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCaa                            >  2:1412352/1‑149 (MQ=255)
                                                                                                                              ggCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTTGCTGACCGGTCACTGTCTGCACGcta                        >  2:696790/1‑149 (MQ=255)
                                                                                                                               gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGTAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGctct                       <  2:288741/150‑1 (MQ=255)
                                                                                                                               gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                         >  1:1214300/1‑148 (MQ=255)
                                                                                                                                 aaaTAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTc                     >  1:1870238/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATGGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGTCCGGTCACTGTCTGCACGCTCTTCGTc                  >  1:1667685/1‑150 (MQ=255)
                                                                                                                                     gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                 >  1:811053/3‑150 (MQ=255)
                                                                                                                                     gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                 >  2:1640533/3‑150 (MQ=255)
                                                                                                                                     gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                 >  1:811059/3‑150 (MQ=255)
                                                                                                                                     aaaaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCCCGCTCTTCGTCa                 >  1:189111/1‑150 (MQ=255)
                                                                                                                                       aaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATAAATTCAGACGAAGCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACTACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg               <  1:103479/150‑1 (MQ=255)
                                                                                                                                       aaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACACCCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg               >  1:1077171/1‑150 (MQ=255)
                                                                                                                                        aaaCGCGCATTATGAGTCCGCCTTTTGCTTTAAGTTTAGAAGATTAAATCCGCCGACCCGGCATCCCGGAGCGGTTTTTAATCGCATCATCAAGCACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg              <  2:127736/150‑1 (MQ=255)
                                                                                                                                         aaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGTCGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGATCAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgtgt             <  2:672263/150‑1 (MQ=255)
                                                                                                                                            gCGGATTATGAGTCCGCCTTTTGCTTTATGTTTTGAATATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAAAAACCTGGTCAACATCTGGCTGACCTGTCACTGTCTGCACGCTCTTCGTAAGGGTAAt          >  1:352180/1‑150 (MQ=255)
                                                                                                                                             cGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAAtt         >  2:1650738/1‑150 (MQ=255)
                                                                                                                                                aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc      <  2:753261/150‑1 (MQ=255)
                                                                                                                                                aTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc      <  2:202081/150‑1 (MQ=255)
                                                                                                                                                aTTAGGAGTGCGCCTTTTGCTTTAAGTTTTGATGATTATTTCAGACGAACGGGCTTCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc      <  2:1190671/150‑1 (MQ=255)
                                                                                                                                                  tATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGcac    <  1:602189/150‑1 (MQ=255)
                                                                                                                                                   aTGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGCCCGGGCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGcaca   >  2:616991/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAA  >  NC_000913/854645‑854942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: