Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 854,796 G→A 5.6% intergenic (+31/+28) ybiR → / ← ldtB putative transporter/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913854,7960GA 5.6% 448.2 / 8.7 142intergenic (+31/+28)ybiR/ldtBputative transporter/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base G (65/68);  new base A (5/3);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATT  >  NC_000913/854647‑854944
                                                                                                                                                     |                                                                                                                                                    
cgcTACTTATGGCTAAAGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTGGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                                                                                                                      <  1:1396360/150‑1 (MQ=255)
  cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTc                                                                                                                                                    <  1:1750083/150‑1 (MQ=255)
   tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGtac                                                                                                                                                   >  2:1091589/1‑148 (MQ=255)
        attgctaaCGATCGCCTCATCTGGTGGCGTTACCATCTCTATTCAATAACGATGCTGTTGTGGGCGGCGTTGGTGGGACATGTTTTGTTAGTTATACTCGCGGCCAACTAGGCTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                                                                                                              <  1:148957/144‑1 (MQ=255)
           gCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttt                                                                                                                                           <  1:1189674/150‑2 (MQ=255)
            cGAACGATCGCCGCGTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                          >  1:1327535/1‑150 (MQ=255)
            cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                          >  1:1607940/1‑150 (MQ=255)
            cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                                                                                                                          >  2:514286/1‑150 (MQ=255)
            cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCATTTGc                                                                                                                                          >  2:752698/1‑150 (MQ=255)
             gAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                                                                                                                         >  1:138514/1‑150 (MQ=255)
                cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                                                                                                                      >  1:753261/1‑150 (MQ=255)
                cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttattt                                                                                                                                      <  2:777844/150‑7 (MQ=255)
                 gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGCAGCTGTCGTGGGCGGCGTTGGTGGGATATGGTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGACTCATAATCCGCCttttttatttg                                                                                                                                     <  2:308250/150‑8 (MQ=255)
                 gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTaa                                                                                                                                     >  1:1145849/1‑150 (MQ=255)
                   tCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGt                                                                                                                                   <  1:1563176/150‑1 (MQ=255)
                     gccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGttt                                                                                                                                 >  1:563036/1‑150 (MQ=255)
                       cgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                                                               <  1:666948/150‑1 (MQ=255)
                       cgcATCTGGTGGCGTTTCCATATCTATTAAATACCTATGCTGTTGTGGGGGGCGTTGGTGGGATATTTTTTGTTAGTTATACTCCCGGACAAGTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                                                               <  2:37694/150‑1 (MQ=255)
                        gcATCTGGTGGCGGTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGa                                                                                                                              <  1:1557125/150‑1 (MQ=255)
                          aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                            <  1:1648808/150‑1 (MQ=255)
                          aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                                                                                                                            <  1:1766196/150‑1 (MQ=255)
                            cTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGGGGGATATGTTTTGTTAGGTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                                                                          <  1:378378/150‑1 (MQ=255)
                             tttgggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGTCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                         <  1:401170/146‑1 (MQ=255)
                             tggtggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAtt                                                                                                                         <  2:1248424/150‑1 (MQ=255)
                                tggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTGGCAAATAAAAAAGGAGGATTATGAGTCCGACTTTTGCTTTAAGTTTTGAAGATTTAt                                                                                                                      >  2:936980/1‑150 (MQ=255)
                                 ggCTTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGAGGCGTTGGTGGGATATGTATTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCATTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                     >  1:1047474/1‑150 (MQ=255)
                                 ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                                                                                                                     <  2:80816/150‑1 (MQ=255)
                                    ggttCCATCTCTATTCAATACCGATGCTGATGTGGGCGTCGTTGGTGGGATATGTTTTGTTAGTTTTACTCACGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                                                                  <  1:1021329/148‑1 (MQ=255)
                                      ttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                                                                >  1:288741/1‑150 (MQ=255)
                                       tCCATCTCTATTCAATACCGATGCTGTTTTGGGCGTCGTTTGTGGGATATGTTTTGTTAGTTATACTCCCTGCCAAATAGTGTCTGGCAAATAAAAAAGGCTGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGAAGATTAATTCATACg                                                                                                               <  2:1429291/150‑1 (MQ=255)
                                           tctcTATTCACAACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                                                                           <  2:1599077/150‑1 (MQ=255)
                                           tctcTATTCAATTCCGATGCGGTTGTGGGCGGCGTTGGTGGGATTTGTTTTGTTAGTTATACTCCCTGCCAACTAGTGGCTGGCAAATAAAAAAGGCGGTTTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                                                                           <  2:1641/150‑1 (MQ=255)
                                              ctATTCAATCCCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                        <  1:1860868/150‑1 (MQ=255)
                                              ctATTCAATACCGCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTCAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                                                                        <  1:1007127/150‑1 (MQ=255)
                                                  tCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGTCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc                                                                                                    >  1:202081/1‑150 (MQ=255)
                                                  tCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc                                                                                                    >  1:7801/1‑150 (MQ=255)
                                                         cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                                                             >  2:1589679/1‑150 (MQ=255)
                                                          gATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCgg                                                                                            >  2:66464/1‑150 (MQ=255)
                                                             gCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttt                                                                                         >  2:1754696/1‑150 (MQ=255)
                                                              cTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtttt                                                                                        >  1:1812399/1‑150 (MQ=255)
                                                               tgttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGGCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                       <  1:632281/150‑1 (MQ=255)
                                                               tgttgtGGGCGGCGGTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCACCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                       <  1:889134/150‑1 (MQ=255)
                                                               gtgtgtgGGCGGCGTGGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGttttt                                                                                       <  2:1029088/147‑1 (MQ=255)
                                                                 ttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCAGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                                                                     <  1:1218754/150‑1 (MQ=255)
                                                                  tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGATATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAAt                                                                                    <  1:426206/150‑1 (MQ=255)
                                                                    tgGGCGGCGTTGTTTGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTTCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCg                                                                                  <  1:844472/150‑1 (MQ=255)
                                                                    tgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCg                                                                                  >  2:803502/1‑150 (MQ=255)
                                                                     gggCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGc                                                                                 >  1:1334499/1‑150 (MQ=255)
                                                                        cggcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtc                                                                              <  2:1607940/150‑1 (MQ=255)
                                                                               ggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGa                                                                       <  2:1309240/150‑1 (MQ=255)
                                                                               ggtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGa                                                                       <  1:886854/150‑1 (MQ=255)
                                                                                gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                                                                      >  2:602189/1‑150 (MQ=255)
                                                                                    gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                                                                  >  2:550264/1‑150 (MQ=255)
                                                                                    gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTATGCTTAAAGTTTTGAAGATTAATTCAGACGAACCGTCATCCCGGAGCGGATTAAAATCGCATCATCAAGaacaac                                                                  >  2:1116009/1‑150 (MQ=255)
                                                                                        tGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGTCGAAACTGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                                                              <  2:913863/150‑1 (MQ=255)
                                                                                        tGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGATGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                                                              >  2:186389/1‑150 (MQ=255)
                                                                                        tGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                                                              <  2:516693/150‑1 (MQ=255)
                                                                                         gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                             >  2:1780984/1‑150 (MQ=255)
                                                                                         gTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                                                             >  1:514362/1‑150 (MQ=255)
                                                                                          gtttGTTAGTTATACTCCCGGCCAAGTAGGGTCTGGCAAATAAATAAGTCTGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAACGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTc                                                            <  2:1047474/149‑1 (MQ=255)
                                                                                           tttGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCa                                                           <  2:493460/150‑1 (MQ=255)
                                                                                              gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCTGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACa                                                        >  2:1057792/1‑150 (MQ=255)
                                                                                                tagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATc                                                      >  2:1760107/1‑150 (MQ=255)
                                                                                                  gttaTACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCAGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGGCAACATCTg                                                    <  2:258768/150‑1 (MQ=255)
                                                                                                  gttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCCTCCAGGAGCGGTTTTTAATCTCTTCATCAAGAACAACCTGGTCAACATCTg                                                    >  2:149814/1‑150 (MQ=255)
                                                                                                    tataCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGc                                                  >  1:830452/1‑150 (MQ=255)
                                                                                                     ataCTCCCGGCCAACTAGGGCCTGGCAAATAAAAAAGCCGGATTATGAGTCTGCATTTTGCTTTAACTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCt                                                 <  2:102345/150‑1 (MQ=255)
                                                                                                      taCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTg                                                <  1:750883/150‑1 (MQ=255)
                                                                                                       aCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGACCGCCTTTTGGTTTAAGTTTTGAAGATTAATACAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTgc                                               >  2:545391/1‑149 (MQ=255)
                                                                                                        cTCCCGGCCAACTAGTGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGAAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                              <  1:364641/150‑1 (MQ=255)
                                                                                                        cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                              >  2:1670509/1‑150 (MQ=255)
                                                                                                        cTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc                                              >  1:677514/1‑150 (MQ=255)
                                                                                                          cccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCg                                            >  1:1126283/1‑150 (MQ=255)
                                                                                                           ccGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCgg                                           <  1:803502/150‑1 (MQ=255)
                                                                                                            cGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGTATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGt                                          <  2:77671/150‑1 (MQ=255)
                                                                                                            cGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGt                                          >  2:579591/1‑150 (MQ=255)
                                                                                                               ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                       <  1:558787/150‑1 (MQ=255)
                                                                                                               ccAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCAc                                       <  2:750155/150‑1 (MQ=255)
                                                                                                                 aaCTAGGGTCTGGCAAATAAAAACGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTg                                     <  2:1353060/150‑1 (MQ=255)
                                                                                                                   atAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGATTTAAGTTTTGAAGATTAATTCAGACGAACCTGCATCACGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTc                                   <  1:275655/149‑1 (MQ=255)
                                                                                                                     aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                                 <  2:1592670/150‑1 (MQ=255)
                                                                                                                     aGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTg                                 <  1:964581/150‑1 (MQ=255)
                                                                                                                        gTCTGGCAAATAAAAAATGCGGATTATGATTCCTCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGACTAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCAc                              <  2:26900/150‑1 (MQ=255)
                                                                                                                        gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCAc                              <  2:419046/150‑1 (MQ=255)
                                                                                                                        gTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCaa                              >  2:1412352/1‑149 (MQ=255)
                                                                                                                            ggCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTTGCTGACCGGTCACTGTCTGCACGcta                          >  2:696790/1‑149 (MQ=255)
                                                                                                                             gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                           <  2:1214300/148‑1 (MQ=255)
                                                                                                                             gCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGct                           >  1:1214300/1‑148 (MQ=255)
                                                                                                                              cAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCtt                        <  2:628892/150‑1 (MQ=255)
                                                                                                                                   gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                   >  2:1640533/3‑150 (MQ=255)
                                                                                                                                   gcaaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCa                   >  1:811059/3‑150 (MQ=255)
                                                                                                                                   aaaaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGACAACATCTGGCTGACCGGTCACTGTCTGCCCGCTCTTCGTCa                   >  1:189111/1‑150 (MQ=255)
                                                                                                                                     aaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATAAATTCAGACGAAGCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACTACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg                 <  1:103479/150‑1 (MQ=255)
                                                                                                                                     aaaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACACCCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAgg                 >  1:1077171/1‑150 (MQ=255)
                                                                                                                                      aaaGGCGGACTCATAATCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGCCGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg                >  2:474444/1‑150 (MQ=255)
                                                                                                                                      aaaCGCGCATTATGAGTCCGCCTTTTGCTTTAAGTTTAGAAGATTAAATCCGCCGACCCGGCATCCCGGAGCGGTTTTTAATCGCATCATCAAGCACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAggg                <  2:127736/150‑1 (MQ=255)
                                                                                                                                         ggCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTAAGACGAACCGGCATCCCGGAGCGGTATTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGTTCACTGTCTGCACGCTCTTCGTAAGGGTaa             >  2:1013831/1‑150 (MQ=255)
                                                                                                                                                 aTGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGcaca     >  2:546267/1‑150 (MQ=255)
                                                                                                                                                   gAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATATGGCTGACCGGTCACAGTCTGCACGCTCTTCTTCAGGGTAATTGGCACaa  >  1:940883/1‑149 (MQ=255)
                                                                                                                                                    aGTCCGCCTTTTGCCTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAAtt  >  1:1818840/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATT  >  NC_000913/854647‑854944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: