Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,576 T→C 5.5% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5760TC 5.5% 367.9 / 9.4 132intergenic (+21/+220)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base T (71/50);  new base C (4/3);  total (76/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 135 total aligned reads displayed.

ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAG  >  NC_000913/857427‑857723
                                                                                                                                                     |                                                                                                                                                   
aTCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAt                                                                                                                                                     <  1:1849650/150‑1 (MQ=255)
aTCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAt                                                                                                                                                     <  1:58164/150‑1 (MQ=255)
  cTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATAc                                                                                                                                                   >  2:1568146/1‑150 (MQ=255)
    ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                 <  2:693120/150‑1 (MQ=255)
     tGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGt                                                                                                                                                <  2:1066989/150‑1 (MQ=255)
        ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCTACCCGCATTATGGAAATCACCCCGGAACGCGGGATCGACTTTAGCGGTAGTTACGAACCTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCgg                                                                                                                                             <  2:1332083/150‑1 (MQ=255)
        ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                             >  2:1177835/1‑150 (MQ=255)
          cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                           >  1:37827/1‑150 (MQ=255)
          cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                           <  1:1802104/150‑1 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                          >  2:50600/1‑150 (MQ=255)
            cacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTg                                                                                                                                         >  1:1309473/1‑150 (MQ=255)
            cacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTg                                                                                                                                         <  2:1617390/150‑1 (MQ=255)
                aCCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcgg                                                                                                                                     >  1:1288251/1‑150 (MQ=255)
                aCCGGGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGCAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAcgg                                                                                                                                     <  2:1527655/150‑1 (MQ=255)
                 ccGGGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggc                                                                                                                                    <  2:462635/150‑1 (MQ=255)
                  cGTGAGTTCGTAAGCGCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAAAGCGGGATCGACTTAAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcg                                                                                                                                   <  1:236985/150‑1 (MQ=255)
                   gTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcgg                                                                                                                                  >  1:1248433/1‑150 (MQ=255)
                   gTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcgg                                                                                                                                  >  1:1821758/1‑150 (MQ=255)
                    tGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCGGGGTGACggcggc                                                                                                                                 >  1:822387/1‑150 (MQ=255)
                      aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCTAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               <  2:1228352/150‑1 (MQ=255)
                      aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               >  2:1139522/1‑150 (MQ=255)
                      aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               >  1:1161103/1‑150 (MQ=255)
                      aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCAGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               >  2:37944/1‑150 (MQ=255)
                       gTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                              >  1:1330064/1‑150 (MQ=255)
                       gTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                              >  2:1540496/1‑150 (MQ=255)
                         tCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatg                                                                                                                            <  2:343022/150‑1 (MQ=255)
                          cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatga                                                                                                                           >  2:537179/1‑150 (MQ=255)
                           gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCGGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGCTGAc                                                                                                                          >  1:91041/1‑150 (MQ=255)
                              aGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACaaa                                                                                                                       >  1:556505/1‑150 (MQ=255)
                               gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAt                                                                                                                      >  2:1038161/1‑150 (MQ=255)
                                cTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATg                                                                                                                     <  2:1330064/150‑1 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                   <  1:996012/150‑1 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                   >  1:1650025/1‑150 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGCGACGGCGGCTGATGACAAATGCa                                                                                                                   >  1:1577970/1‑150 (MQ=255)
                                   ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaa                                                                                                                  <  1:234853/150‑1 (MQ=255)
                                   ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaa                                                                                                                  >  1:1674097/1‑150 (MQ=255)
                                    gtGGCGACCCGAATTCTGTAAATCACCCCGGAACGGGTGAGCGACTTTAGGGTAAATTACGTCGATTACGTGCGTAGTAAAGGTATCGTGTAAGTGATTAACGGTCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  2:411571/149‑1 (MQ=255)
                                    cTGGCGACCCGCATTCTGTAAATCACCCCGGAACGCGTCATCGACTTTAGCGGTAATTCCGAAGATTACCTGCGTAGTAAAGGGATCGAGAAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  2:177040/150‑1 (MQ=255)
                                    cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGTTCGACTTTATCGGTAATTACGACGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  1:348682/150‑1 (MQ=255)
                                    cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCaaa                                                                                                                 >  2:1160624/1‑150 (MQ=255)
                                    cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGAGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGCGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  2:246130/150‑1 (MQ=255)
                                     tGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAAt                                                                                                               >  1:720843/1‑150 (MQ=255)
                                       gCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATa                                                                                                              <  2:1073389/150‑1 (MQ=255)
                                          aCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGcc                                                                                                           >  1:28033/1‑150 (MQ=255)
                                           cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                          >  2:79603/1‑150 (MQ=255)
                                           cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                          >  1:1100772/1‑150 (MQ=255)
                                            ccGCATTCTGGAAATCACCCCGGAACGCGTGATCAACTTTAGCGGTAATTTCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTg                                                                                                         >  1:1444106/1‑150 (MQ=255)
                                                   cTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCt                                                                                                  <  1:26318/150‑1 (MQ=255)
                                                   cTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCt                                                                                                  >  2:1309250/1‑150 (MQ=255)
                                                     ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTAc                                                                                                >  1:1631661/1‑150 (MQ=255)
                                                      gAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACg                                                                                               >  1:687243/1‑150 (MQ=255)
                                                      gAAATCACCCCGCAACGCGTGATCGACTTAAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACg                                                                                               <  2:982468/150‑1 (MQ=255)
                                                             ccccGCACCGCGTTAGCGACTTTAGCGGTAATTACCAATATTACCTGCTTTTAAATGGGAGGGATTACGTGATTACCCGCCACCCCCAAACGGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCa                                                                                        <  2:1187440/150‑1 (MQ=19)
                                                              cccGGAACGCGTGATAGACTTTAGCGGTAATTACGAAGAATACCTGAGTAGTATAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCGGGGTGCCGGCGGCTGATGACAAATGAAAAATAGCATGATGCGCTCCGCTGAACAg                                                                                       >  2:323773/1‑150 (MQ=255)
                                                                cGGAACGCGTGATCGACTTAGCTGTAATTCCGAAGTTTACCTGCGTAGTAAAGGGAACGAGTAAGTGATAATCCGTCACCCCGATACTGTCGTTGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGcc                                                                                    <  1:954130/150‑1 (MQ=38)
                                                                     cgcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                                >  2:1594389/1‑150 (MQ=255)
                                                                               aCTTTAGCGGAAATACCGACTATTCCCTGCGTATAAAAGGTATCGAGTATGTTATTAACCGACACCCCGTTACTGTCGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                      <  2:376996/150‑1 (MQ=25)
                                                                                  ttAGAGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCATAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAat                                                                   <  1:991734/150‑1 (MQ=255)
                                                                                     gCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatata                                                                >  2:1859034/1‑150 (MQ=255)
                                                                                       ggTAATTACTAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGGTATTATCCGTCACCCCGATACTGCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                              <  2:837580/150‑1 (MQ=38)
                                                                                       ggTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCCACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                              >  2:217670/1‑150 (MQ=255)
                                                                                            ttACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATc                                                         >  1:1684776/1‑150 (MQ=255)
                                                                                               ctaattttACCTGCGTAGTAAAGTGATCGAGTAATTGATTTTCCGTCACACCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCTCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                      <  2:988029/144‑1 (MQ=37)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                      <  2:708105/150‑1 (MQ=255)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAAAAGCCTGCTGCGCTACGCTTATCAGGCCTCCGCAGTACCTGCAATATAGTGAATCTGc                                                      >  1:109345/1‑150 (MQ=255)
                                                                                                 aaGATAACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAg                                                    <  1:1498198/150‑1 (MQ=255)
                                                                                                  aGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGAACCTGCAATATATTGAATCTGCAgg                                                   <  1:1223292/150‑1 (MQ=255)
                                                                                                        ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTg                                             >  1:123880/1‑150 (MQ=255)
                                                                                                        ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTg                                             >  2:1614964/1‑150 (MQ=255)
                                                                                                          tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                           >  1:1783232/1‑150 (MQ=255)
                                                                                                           gcttagtaAAGGGATCGAGTAAGTGATTATCCGGCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAg                                          <  2:194898/147‑1 (MQ=255)
                                                                                                           gCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAg                                          >  2:315458/1‑150 (MQ=255)
                                                                                                               agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                      <  2:1309473/150‑1 (MQ=255)
                                                                                                                  aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                   >  1:1572869/1‑150 (MQ=255)
                                                                                                                   aaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                  >  2:385792/1‑150 (MQ=255)
                                                                                                                     gggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATacg                                >  1:1782994/1‑148 (MQ=255)
                                                                                                                       gATCGCGTAAGGGATTATCCGTCACCCCGATCCTGTCGGGGTGACGGCGGCTGATGACAACTGCAAAATAGCCTTATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                              <  1:954722/150‑1 (MQ=37)
                                                                                                                        aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                             >  2:934632/1‑150 (MQ=255)
                                                                                                                           gAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTa                          >  1:1139437/1‑150 (MQ=255)
                                                                                                                                aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                     <  2:720843/150‑1 (MQ=255)
                                                                                                                                 gTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                    <  1:50600/150‑1 (MQ=255)
                                                                                                                                  tGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                   >  1:479130/1‑150 (MQ=255)
                                                                                                                                   gcttATCCGTCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                  <  2:883630/148‑1 (MQ=255)
                                                                                                                                   gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                  <  2:1821758/150‑1 (MQ=255)
                                                                                                                                        gccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGATAACGCCGCATCAgg             >  2:701351/2‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCCGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGCCTGTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc            >  2:498261/1‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGGATCAGGc            >  1:347172/1‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc            >  2:394531/1‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc            >  1:1711406/1‑150 (MQ=255)
                                                                                                                                           gTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt          >  1:1577163/1‑150 (MQ=255)
                                                                                                                                             ccccccGCTAGTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATCGCCTGATGCGCTACGCTTATCAGGCATACGCAGTACCTGCAATATAATGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa        <  2:1127252/148‑1 (MQ=25)
                                                                                                                                             ccccccGATACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa        <  1:1160624/148‑1 (MQ=255)
                                                                                                                                             ccccccGAAACTGGCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa        <  1:537179/148‑1 (MQ=255)
                                                                                                                                               ccccGATGCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATATGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  2:358656/1‑150 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  1:1159705/1‑150 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  2:1074969/1‑150 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  2:1422884/1‑150 (MQ=255)
                                                                                                                                               ccccGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  1:1283679/1‑150 (MQ=255)
                                                                                                                                                  cGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaaa   <  2:1657815/150‑1 (MQ=255)
                                                                                                                                                   taTACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAg  <  2:822151/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAG  >  NC_000913/857427‑857723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: