Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 881,123 G→A 5.3% G133S (GGT→AGT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913881,1230GA 5.3% 330.2 / 5.7 113G133S (GGT→AGT) dacCD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6a
Reads supporting (aligned to +/- strand):  ref base G (50/57);  new base A (3/3);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 119 total aligned reads displayed.

TAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGC  >  NC_000913/880975‑881272
                                                                                                                                                    |                                                                                                                                                     
tAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAATCCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCgg                                                                                                                                                      <  2:548086/150‑1 (MQ=255)
    aTTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt                                                                                                                                                  <  1:1713308/150‑1 (MQ=255)
     ttACACTCTACGATTTGGTGACGGTCGGTAAAGATGCCTGGGCGCCGGGAAATCCGGCACTGCGTGTTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGTGGTGATTATCCAGTCCGGTAATg                                                                                                                                                 <  1:947285/150‑1 (MQ=255)
     ttAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATg                                                                                                                                                 <  2:421083/150‑1 (MQ=255)
     taacaCTCACAGATATGGTGAAGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGGGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATg                                                                                                                                                 <  2:1650864/146‑1 (MQ=255)
      tAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTACGGTAAtca                                                                                                                                                >  1:170457/1‑148 (MQ=255)
        aaCTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACg                                                                                                                                              <  1:1568357/150‑1 (MQ=255)
            cACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTg                                                                                                                                          <  2:1784172/150‑1 (MQ=255)
            cACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGCTAATGACGCCTg                                                                                                                                          >  2:1069337/1‑150 (MQ=255)
             aCCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAACCCGGGCGCTCAGGTGGCGGTGGCCGACTTGCACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGt                                                                                                                                         >  2:1179190/1‑150 (MQ=255)
              ccGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGCCTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTa                                                                                                                                        >  2:821765/1‑150 (MQ=255)
              ccGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCATCAACCAGGGCGATCAGGTTTCGGAGGCATACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGACTGTa                                                                                                                                        >  1:189059/1‑150 (MQ=255)
                gATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATACAGTCCGGTAATGACGCCTGTAtt                                                                                                                                      >  2:1834349/1‑150 (MQ=255)
                 atatGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGcc                                                                                                                                            <  1:1155809/143‑1 (MQ=255)
                 atatGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGcc                                                                                                                                            >  2:1155809/1‑143 (MQ=255)
                 atatGGTGACGGGCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTGCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTg                                                                                                                                     <  2:991926/150‑1 (MQ=255)
                    tGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTgcgc                                                                                                                                  >  2:450006/1‑150 (MQ=255)
                       tGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCAGTAATGACGCCTGTATTGCgctgg                                                                                                                               >  1:558383/1‑150 (MQ=255)
                       tGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCAGTAATGACGCCTGTATTGCgctgg                                                                                                                               >  1:374862/1‑150 (MQ=255)
                       tGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCAGTAATGACGCCTGTATTGCgctgg                                                                                                                               >  1:381849/1‑150 (MQ=255)
                          cggtcggtAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCgctggctg                                                                                                                            <  1:414068/150‑1 (MQ=255)
                               ggtAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGAGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTAc                                                                                                                       >  2:1079717/1‑150 (MQ=255)
                                gtAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGGTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACTAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACg                                                                                                                      <  2:1036473/150‑1 (MQ=255)
                                 tAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGt                                                                                                                     <  2:1007099/150‑1 (MQ=255)
                                    aGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGc                                                                                                                  <  1:1951/150‑1 (MQ=255)
                                      aTGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCTGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCg                                                                                                                <  1:763385/150‑1 (MQ=255)
                                          cTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCGGATTACGTTGCCGGGAg                                                                                                            >  2:90520/1‑150 (MQ=255)
                                          cTGGGCGACGGGAAATCCGGCACTGCGGGGTGCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAg                                                                                                            <  1:892939/150‑1 (MQ=255)
                                           tGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGc                                                                                                           <  1:1831117/150‑1 (MQ=255)
                                           ggggCGACGGGAAATCCGGCACTGCGGGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGACTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGc                                                                                                           <  1:1400956/149‑1 (MQ=255)
                                               cGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGATTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAgg                                                                                                       >  1:142302/1‑150 (MQ=255)
                                               cGACGGGAAATCCGGCACGGCGTGGTGCATCGGTAATGTTCCTCAAACCGGGCGAGCAGGTTTCGGGGGCAGACTTGAACAAAGGGGGGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAgg                                                                                                       <  1:1379692/150‑1 (MQ=255)
                                                 aCGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAg                                                                                                     <  1:1307854/150‑1 (MQ=255)
                                                    ggAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCa                                                                                                  >  1:373241/1‑150 (MQ=255)
                                                    ggAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCa                                                                                                  >  2:1336444/1‑150 (MQ=255)
                                                     gAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCAt                                                                                                 >  2:377885/1‑150 (MQ=255)
                                                         tCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTAt                                                                                             >  2:796664/1‑150 (MQ=255)
                                                          ccGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCAGTTAtt                                                                                            >  2:1561886/1‑150 (MQ=255)
                                                           cGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTg                                                                                           >  1:1370822/1‑150 (MQ=255)
                                                              cACTGCGTGGTTCATCGGTAATGTTACTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCAAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATGTATTGGTc                                                                                        >  2:1404876/1‑150 (MQ=255)
                                                               aCTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCt                                                                                       <  1:570338/150‑1 (MQ=255)
                                                                 tGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGCTCAGGGTTCGGTGGCAGACTTGAACAAAGGTGTGAATATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCtga                                                                                     <  2:727093/150‑1 (MQ=255)
                                                                 tGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGAATACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCtga                                                                                     <  1:1560368/150‑1 (MQ=255)
                                                                  gCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCtgat                                                                                    >  1:142937/1‑150 (MQ=255)
                                                                      ggTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAAt                                                                                >  2:1272794/1‑150 (MQ=255)
                                                                       gtgcctCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGTCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATg                                                                               <  2:1078519/145‑1 (MQ=255)
                                                                       gTTCATCTGTAATGTTCCTCAAACCGGTCGATCAGGTTTCGGTGGGAGTCTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATg                                                                               <  1:1682413/150‑1 (MQ=255)
                                                                       gTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATg                                                                               <  2:1074021/150‑1 (MQ=255)
                                                                             gggTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATg                                                                         <  1:815314/149‑1 (MQ=255)
                                                                             ctgTAATGTTCCTAAAACCGGGCGAGACGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATACAGTCCGTTAATGTCGCCTGTATTGCGATGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTTGTCTGATGAATGGTTATg                                                                         <  1:931754/148‑1 (MQ=255)
                                                                             cGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACTAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATg                                                                         <  1:906659/150‑1 (MQ=255)
                                                                             cGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATg                                                                         >  2:1528971/1‑150 (MQ=255)
                                                                                tAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGTCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCa                                                                      >  2:1226498/1‑150 (MQ=255)
                                                                                tAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCa                                                                      <  1:1098060/150‑1 (MQ=255)
                                                                                   tGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCaaaa                                                                   <  1:204646/150‑1 (MQ=255)
                                                                                    gTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGAAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCaaaaa                                                                  <  2:912683/150‑1 (MQ=255)
                                                                                      tCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCAGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCaaaaaaa                                                                >  2:663992/1‑149 (MQ=255)
                                                                                       ccTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACt                                                               >  2:1685299/1‑150 (MQ=255)
                                                                                        cTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGCGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTg                                                              >  2:963745/1‑150 (MQ=255)
                                                                                         tCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTgg                                                             <  1:757968/150‑1 (MQ=255)
                                                                                         tCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTgg                                                             <  1:450006/150‑1 (MQ=255)
                                                                                          cAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAATGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGCTATGCCAAAAAACTggg                                                            <  2:472734/150‑1 (MQ=255)
                                                                                            aaCCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTc                                                          <  1:823689/150‑1 (MQ=255)
                                                                                              ccGGGCGATCAGGTTTCGGTGGCAGACTTGACCAAAGGGGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTCGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTg                                                        <  2:566035/150‑1 (MQ=255)
                                                                                              ccGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATCACGCCTGTATTGCGCCGGCTGAGTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTg                                                        <  2:107021/150‑1 (MQ=255)
                                                                                                gggCGATCAGGTTTCGGTGGAAGACTTGAACAAATGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTTGCTGATTACGTTGCCGTGAGGCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCTAAAAACTGGGTATGAc                                                      <  2:621322/150‑1 (MQ=255)
                                                                                                gcccGATCAGGTTTCGGTGGCAGACTTGAACAAAGCTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCAGCCTGATTACGTAGACGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTAAGCCAAAAAACTGGGGCTGAc                                                      <  1:271728/147‑1 (MQ=255)
                                                                                                 ggCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCAGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGAcc                                                     <  2:374862/150‑1 (MQ=255)
                                                                                                   cGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCaa                                                   >  2:981608/1‑150 (MQ=255)
                                                                                                   cGATCAGGCTTCGGTGGCAGACTTGAACAATGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATAGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCATAAAACTGGGTCTGACCaa                                                   <  2:1731707/150‑1 (MQ=255)
                                                                                                    gATCAGGTTTCGGTGGCAGACTTGAACAAAGGGGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAac                                                  <  2:978390/150‑1 (MQ=255)
                                                                                                     aTCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAaca                                                 >  1:668694/1‑150 (MQ=255)
                                                                                                       cAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACt                                               >  2:888728/1‑150 (MQ=255)
                                                                                                          gTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTAcc                                            >  2:1094026/1‑150 (MQ=255)
                                                                                                          gTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTAcc                                            >  1:847216/1‑150 (MQ=255)
                                                                                                              cGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTcc                                        <  2:142302/150‑1 (MQ=255)
                                                                                                              cGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTcc                                        <  1:599368/150‑1 (MQ=255)
                                                                                                                   gCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACg                                   <  1:1834349/150‑1 (MQ=255)
                                                                                                                      gACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCGGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTg                                <  2:1650544/150‑1 (MQ=255)
                                                                                                                           gAACAAAGTTGTTATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGTGTCTGACCAACACTACCTTCCAGACGGTGCACgg                           <  1:550544/150‑1 (MQ=255)
                                                                                                                           gAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACgg                           >  1:1181448/1‑150 (MQ=255)
                                                                                                                           gAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACgg                           >  1:218650/1‑150 (MQ=255)
                                                                                                                                     gtgATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGGCCGTGCACCGTCTGGaaggt                 <  1:1350820/150‑5 (MQ=255)
                                                                                                                                      tgATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATgcgc                <  1:1069337/150‑1 (MQ=255)
                                                                                                                                       gATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCACCACTACCTTCCAGACGGTGCCCGGCCTGGATGCGcc               >  1:1132862/1‑150 (MQ=255)
                                                                                                                                        aTTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCg              >  2:1416224/1‑150 (MQ=255)
                                                                                                                                         ttATCCAGTCCGGTACTGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGGCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCAGGATGCGCCgg             <  1:821765/150‑1 (MQ=255)
                                                                                                                                          tATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCggg            >  2:1330163/1‑150 (MQ=255)
                                                                                                                                             ccAGTCCGGTAATGACGCCTGTATTGCGCTGGCTAATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCa         >  2:1028533/1‑150 (MQ=255)
                                                                                                                                              cAGTCCGGTAATGCCGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCC‑‑AAAACTGGGTCTGACCAACCCTACCTTCCAGACGGTGCCCGGCCTGGCTGCGCCGGGGCAGtg      >  2:503565/1‑149 (MQ=255)
                                                                                                                                              cAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTTGATGCGCCGGGGCAg        >  1:306788/1‑150 (MQ=255)
                                                                                                                                                 tCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTc     <  1:1524067/150‑1 (MQ=255)
                                                                                                                                                  ccGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCTGGGAGCCAGGAGTCATTCATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCa    >  2:1270999/1‑150 (MQ=255)
                                                                                                                                                  ccGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCa    >  2:1544737/1‑150 (MQ=255)
                                                                                                                                                  ccGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCa    <  2:970022/150‑1 (MQ=255)
                                                                                                                                                    ggTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACCCTCCCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGc  >  2:323474/1‑150 (MQ=255)
                                                                                                                                                    ggTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGc  >  2:686718/1‑150 (MQ=255)
                                                                                                                                                    ggTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGc  >  2:536462/1‑150 (MQ=255)
                                                                                                                                                    ggTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGc  >  1:1068102/1‑150 (MQ=255)
                                                                                                                                                    ggTAATGACGCATGTATTGCGCTGGCTGATTAGGTTGCCGGGAGCCAGGAGTCATTTATTGTGCTGTTGAATGGTAATGACAAAAAACTGGGTCTGACCAACAGTTCCTTCCAGACGGTGCTCGGCCTGGATGCGCCGGGGCAGTTCAGc  <  2:189059/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGC  >  NC_000913/880975‑881272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: