Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 934,195 T→A 5.5% A324A (GCT→GCA ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913934,1950TA 5.5% 259.3 / 2.7 95A324A (GCT→GCAftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base T (47/42);  new base A (2/4);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GACGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGC  >  NC_000913/934046‑934344
                                                                                                                                                     |                                                                                                                                                     
gaCGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGTCCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgct                                                                                                                                                       <  2:1486299/150‑1 (MQ=255)
gaCGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTCTTAAACGGTGCGCCAATTACCGACCCTGTCGCTGTAGCAgct                                                                                                                                                       <  2:1129054/150‑1 (MQ=255)
gaCGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgct                                                                                                                                                       <  2:95241/150‑1 (MQ=255)
 aCGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctg                                                                                                                                                      >  1:1743603/1‑150 (MQ=255)
  cGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgc                                                                                                                                                     >  2:1014270/1‑150 (MQ=255)
  cGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgc                                                                                                                                                     >  1:429610/1‑150 (MQ=255)
  cGAAGAGATTACCTACACTGCACGCGGTCTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGGCGAATACGATCCCTTTTTAAAAGGTGCGCCAATAACCGTACCTGTCGCTGTAGCAgctgc                                                                                                                                                     >  2:484754/1‑150 (MQ=255)
   gAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGCTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgct                                                                                                                                                    <  2:1682048/150‑1 (MQ=255)
     agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                  <  1:545431/150‑1 (MQ=255)
     agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                  <  1:1532499/150‑1 (MQ=255)
     agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                  <  2:128753/150‑1 (MQ=255)
     agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                  >  1:1375406/1‑150 (MQ=255)
      gagaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgct                                                                                                                                                 <  2:1582963/150‑1 (MQ=255)
       tgaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTa                                                                                                                                                <  2:1226756/149‑1 (MQ=255)
       agaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCGGCTGCTGCTa                                                                                                                                                >  1:1346695/1‑150 (MQ=255)
       agaTTACCTACACAGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGGAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTa                                                                                                                                                <  1:1797818/150‑1 (MQ=255)
        gaTTACCTAAACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCAGTATTAAGCGGTGCGCCACGTACCGAACCTGTCGCTGTAGCAGCTGCTGCTAc                                                                                                                                               >  1:1156004/1‑150 (MQ=255)
          ttACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCa                                                                                                                                             >  2:1516198/1‑150 (MQ=255)
          ttACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCa                                                                                                                                             >  2:1142017/1‑150 (MQ=255)
           tACCTACACTGCGCGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCAc                                                                                                                                            >  2:870818/1‑150 (MQ=255)
            aCCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGATTATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCGACCACg                                                                                                                                           >  2:799145/1‑150 (MQ=255)
             ccTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGATTATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACgg                                                                                                                                          <  2:1681799/150‑1 (MQ=255)
              cTACACTGCACGCGGTGGGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGc                                                                                                                                         <  1:1078095/150‑1 (MQ=255)
                acacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGa                                                                                                                                       <  2:693175/150‑1 (MQ=255)
                 cacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGac                                                                                                                                      >  1:1747203/1‑150 (MQ=255)
                 cacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGac                                                                                                                                      <  2:1842838/150‑1 (MQ=255)
                   cTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacag                                                                                                                                    <  1:1008783/150‑2 (MQ=255)
                    tGCACGCGGTTTGGCTGCCGACACGGACGTAGTACTATTTTAGGTCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGGGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                   <  1:371528/150‑3 (MQ=25)
                    tGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                   <  2:1200487/150‑3 (MQ=38)
                    tGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                   <  2:1438970/150‑3 (MQ=38)
                             tgtgGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGc                                                                                                                          <  2:659797/150‑1 (MQ=255)
                                ggCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCttc                                                                                                                       >  1:1180282/1‑148 (MQ=255)
                                ggCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTgc                                                                                                                       >  2:1454608/1‑150 (MQ=255)
                                  cTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTgcgc                                                                                                                     >  2:353609/1‑150 (MQ=255)
                                   tGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGcc                                                                                                                    <  2:1505754/150‑1 (MQ=255)
                                    gCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTTCCGACCCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCg                                                                                                                   >  1:952216/1‑150 (MQ=255)
                                    gCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGGTGATACCACGGCGACACAAAGCTGGGCTGCGCCg                                                                                                                   >  2:167073/1‑150 (MQ=255)
                                       gACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGCCACAAAGCTGGGCTGCGCCGGtt                                                                                                                >  2:1682065/1‑150 (MQ=255)
                                        aCCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTg                                                                                                               <  2:531907/150‑1 (MQ=255)
                                          ccGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGaa                                                                                                             <  2:1530064/150‑1 (MQ=255)
                                           cGGACGTCGGCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGGTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAAc                                                                                                            <  2:228380/150‑1 (MQ=255)
                                             gacgacGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCt                                                                                                          >  2:496965/1‑150 (MQ=255)
                                                  cgTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGAc                                                                                                     <  1:1742467/150‑1 (MQ=255)
                                                     ccTATTTTCGGGCAATCGTGCATCGCAGCCAGAATATGACGAATACGATCGATTATTAAACGGTGCGCAAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACTAAGCTGGGCTCCGCCTGGTGAACCTGTGACTCa                                                                                                  >  2:384789/1‑150 (MQ=255)
                                                      cTATTTTCGGGCAGTCGTGCAACGCAGCCAGAATATGACGAATACGATGCAGTATTAAAAGGTGGGACAATTACCGAACCTGTCGCTGTAGAAGCAGCAGCTACCACGGCGACACAAAGCTGGGCTGCGAAGGTTGACCCTGTGACACAg                                                                                                 >  2:166557/1‑150 (MQ=255)
                                                         ttttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcg                                                                                              >  2:533741/1‑150 (MQ=255)
                                                           ttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcgcc                                                                                            >  1:657965/1‑150 (MQ=255)
                                                           ttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcgcc                                                                                            <  2:1589570/150‑1 (MQ=255)
                                                               ggCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCt                                                                                        >  2:473976/1‑150 (MQ=255)
                                                                 cAATCGTGCAACGCAGCCAGAATATGACGAATACGAGCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCGGGGCTGCGCCGGTTGAACCTGTGCCTCAGACGCCGCCTGt                                                                                      >  1:905290/1‑150 (MQ=255)
                                                                  aaTCGTGCAACGCATCAATAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGtt                                                                                     <  1:554952/150‑1 (MQ=255)
                                                                     cGTGCAACGCAGCCAGAATATGACGAATCCGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGcc                                                                                  <  2:1564631/150‑1 (MQ=255)
                                                                     cGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGCACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCCGACGCCGCCTGTTGcc                                                                                  >  1:1139508/1‑150 (MQ=255)
                                                                      gTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCct                                                                                 >  1:469656/1‑150 (MQ=255)
                                                                         cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTTCTACCACGGCGACACAAAGCTGGGCTGCGCCGTTTGAACCTGTGACTCAGACGCCGCCTGGTGCCGCTg                                                                              >  2:329867/1‑150 (MQ=255)
                                                                         cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACgccgcc                                                                                         <  1:1307477/139‑1 (MQ=255)
                                                                         cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACgccgcc                                                                                         >  2:1307477/1‑139 (MQ=255)
                                                                           aCGCAGCCAGAATATGAAGAATCCTATCCATTATTCAACGGTGCGCCACTTACCGCACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGtt                                                                            <  2:916425/150‑1 (MQ=255)
                                                                             gCAGACAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGa                                                                          <  1:1232610/150‑1 (MQ=255)
                                                                               aGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATg                                                                        <  1:1735973/150‑1 (MQ=255)
                                                                               aGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATg                                                                        <  2:602210/150‑1 (MQ=255)
                                                                                      atatGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTTATGTTCCAcc                                                                 >  1:1710962/1‑150 (MQ=255)
                                                                                      atatGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCAcc                                                                 <  2:1743603/150‑1 (MQ=255)
                                                                                           aCGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTgcgc                                                            <  1:1407308/150‑1 (MQ=255)
                                                                                                  cGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTAc                                                     >  2:1241149/1‑150 (MQ=255)
                                                                                                  cGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTAc                                                     >  2:1045551/1‑150 (MQ=255)
                                                                                                    agccATTATTAACCGGTGCGCCAATTACCGAACCTGGCGCTGTAGCAGCTGCGGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                   <  1:455605/148‑1 (MQ=255)
                                                                                                    aTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                   >  1:1102830/1‑150 (MQ=255)
                                                                                                    aTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                   <  1:1454608/150‑1 (MQ=255)
                                                                                                        attattAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGc                                               <  2:1769294/150‑1 (MQ=255)
                                                                                                         ttattaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGCTGTTCCACCTGCGCAACCTACAGTAGcc                                              >  1:1217054/1‑150 (MQ=255)
                                                                                                           attaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTg                                            <  1:617979/150‑1 (MQ=255)
                                                                                                           attaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCAAGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCGTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTg                                            >  2:717823/1‑150 (MQ=255)
                                                                                                            ttaAACGGTGCGCCAATTACCGAACGTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTgg                                           <  1:97008/150‑1 (MQ=255)
                                                                                                              aaaCGGTGCGCCAATTACAGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCTCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCAggaa                                         >  1:193972/1‑148 (MQ=255)
                                                                                                               atcGGAGCGCCAATTACAGAACCTGTCTCTTTAGCAGCTGCTGCCACCACGGCGACACAACGCTGGGCTGCGCCGGTTGAACCTGTTACTCATACGCCGCCTGATGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAg                                        <  2:936335/148‑1 (MQ=255)
                                                                                                               aaCGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCCCCTGCGCAACCTACAGTAGCCTGGCAg                                        >  1:1818755/1‑150 (MQ=255)
                                                                                                               aaCGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAg                                        <  1:803427/150‑1 (MQ=255)
                                                                                                                ccGGTGCGCGAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGc                                       <  2:186998/149‑1 (MQ=255)
                                                                                                                  ggTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACTAATCTGGGCTGCGCCGGATGAACCTGTGACTCATACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCt                                     >  1:144731/1‑150 (MQ=255)
                                                                                                                   gTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTg                                    <  2:1734912/150‑1 (MQ=255)
                                                                                                                   gTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTg                                    <  1:719190/150‑1 (MQ=255)
                                                                                                                    tGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGt                                   >  2:412846/1‑150 (MQ=255)
                                                                                                                       gcCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTAcc                                >  2:690667/1‑150 (MQ=255)
                                                                                                                         cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGCACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCgg                              >  2:227672/1‑150 (MQ=255)
                                                                                                                         cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCACCCTACAGTAGCCTGGCAGCCTGTACCgg                              >  2:907385/1‑150 (MQ=255)
                                                                                                                         cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCgg                              >  1:1074032/1‑150 (MQ=255)
                                                                                                                         cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCgg                              >  1:836643/1‑150 (MQ=255)
                                                                                                                           aTTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGt                            <  1:313276/150‑1 (MQ=255)
                                                                                                                             tACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTcc                          <  1:3617/150‑1 (MQ=255)
                                                                                                                              aCCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGCGTccc                         >  1:85835/1‑149 (MQ=255)
                                                                                                                               ccGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGCACGTGTGGCGCAGACGCCGCCGGTTGCCGCTGTTGATGTTCCACCTGCGCCACCGACAGTAGCCTGGCAGCCTGTCCCGGGTcgcc                        >  2:319902/1‑147 (MQ=255)
                                                                                                                               agctttgtGTCGCCGTGGTAGCAGCAGCTGCTACAGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGGACCCTACAGTCGCCTGGCGGCGTGTACCGGGTCcac                        >  1:1300086/8‑150 (MQ=25)
                                                                                                                                      tggcGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACggg                 <  2:596494/147‑1 (MQ=255)
                                                                                                                                      gggcgcgGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACggg                 <  1:1430157/147‑1 (MQ=255)
                                                                                                                                      ggTCGCGGTCCCAGCTGCGGCTACCACGGCGACACAAAGCGGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCGCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACtgg                 <  2:170477/149‑1 (MQ=255)
                                                                                                                                         cGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGgagc              >  1:1042249/1‑149 (MQ=255)
                                                                                                                                           cTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCAGGTTGAACCTGTGACTCAGACGCCGCCTGTTGGCTCTGTTGATGTTCCACCTGCGCAACCTACTGTAGCCTGGCAGCCTGTCCCGGGTCCACAAACGGGAGAGc            >  2:1166795/1‑150 (MQ=255)
                                                                                                                                                  agcTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTAt     <  2:1747203/150‑1 (MQ=255)
                                                                                                                                                    gtgctgctACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACTGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTg   <  2:1180282/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACGAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGC  >  NC_000913/934046‑934344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: