Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 934,198 T→A 5.5% A325A (GCT→GCA ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913934,1980TA 5.5% 262.3 / 2.7 95A325A (GCT→GCAftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base T (47/40);  new base A (2/4);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGCTCC  >  NC_000913/934049‑934347
                                                                                                                                                     |                                                                                                                                                     
gAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGCTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgct                                                                                                                                                       <  2:1682048/150‑1 (MQ=255)
  agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                     <  2:128753/150‑1 (MQ=255)
  agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                     <  1:1532499/150‑1 (MQ=255)
  agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                     >  1:1375406/1‑150 (MQ=255)
  agagATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgc                                                                                                                                                     <  1:545431/150‑1 (MQ=255)
   gagaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAgctgctgct                                                                                                                                                    <  2:1582963/150‑1 (MQ=255)
    tgaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTa                                                                                                                                                   <  2:1226756/149‑1 (MQ=255)
    agaTTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCGGCTGCTGCTa                                                                                                                                                   >  1:1346695/1‑150 (MQ=255)
    agaTTACCTACACAGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGGAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTa                                                                                                                                                   <  1:1797818/150‑1 (MQ=255)
     gaTTACCTAAACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCAGTATTAAGCGGTGCGCCACGTACCGAACCTGTCGCTGTAGCAGCTGCTGCTAc                                                                                                                                                  >  1:1156004/1‑150 (MQ=255)
       ttACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCa                                                                                                                                                >  2:1516198/1‑150 (MQ=255)
       ttACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCa                                                                                                                                                >  2:1142017/1‑150 (MQ=255)
        tACCTACACTGCGCGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCAc                                                                                                                                               >  2:870818/1‑150 (MQ=255)
         aCCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGATTATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCGACCACg                                                                                                                                              >  2:799145/1‑150 (MQ=255)
          ccTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGATTATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACgg                                                                                                                                             <  2:1681799/150‑1 (MQ=255)
           cTACACTGCACGCGGTGGGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGc                                                                                                                                            <  1:1078095/150‑1 (MQ=255)
             acacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGa                                                                                                                                          <  2:693175/150‑1 (MQ=255)
              cacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGac                                                                                                                                         <  2:1842838/150‑1 (MQ=255)
              cacTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGac                                                                                                                                         >  1:1747203/1‑150 (MQ=255)
               acTGCTCGCGGTGGGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCTTGCAACGAAGCCAGAATATTACGAATACGATCCATTTTTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGaca                                                                                                                                        <  2:1074696/150‑1 (MQ=255)
                cTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacag                                                                                                                                       <  1:1008783/150‑2 (MQ=255)
                 tGCACGCGGTTTGGCTGCCGACACGGACGTAGTACTATTTTAGGTCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGGGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                      <  1:371528/150‑3 (MQ=25)
                 tGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                      <  2:1200487/150‑3 (MQ=38)
                 tGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCCGTGGTAGCAGCAGCTGCTACAGCGacagg                                                                                                                                      <  2:1438970/150‑3 (MQ=38)
                          tgtgGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGc                                                                                                                             <  2:659797/150‑1 (MQ=255)
                          gggggCTGCCGACCCGGACGACGTCCTATTTTCGGGCACTCGTGAAACGCAGCCAGAATATGCCGAATACGATCCATTATTACCCGGTGCGCCACTTCCCGCACCTGGCGATGTAGAAGCTTCTGCTACCACGGCGACACAAAGCTGGGc                                                                                                                             <  2:666399/147‑1 (MQ=255)
                             ggCTGCCGACCCGGACGCCGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTgc                                                                                                                          >  2:405495/1‑150 (MQ=255)
                             ggCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCttc                                                                                                                          >  1:1180282/1‑148 (MQ=255)
                             ggCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTgc                                                                                                                          >  2:1454608/1‑150 (MQ=255)
                                tGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGcc                                                                                                                       <  2:1505754/150‑1 (MQ=255)
                                 gCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTTCCGACCCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCg                                                                                                                      >  1:952216/1‑150 (MQ=255)
                                 gCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGGTGATACCACGGCGACACAAAGCTGGGCTGCGCCg                                                                                                                      >  2:167073/1‑150 (MQ=255)
                                    gACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGCCACAAAGCTGGGCTGCGCCGGtt                                                                                                                   >  2:1682065/1‑150 (MQ=255)
                                     aCCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTg                                                                                                                  <  2:531907/150‑1 (MQ=255)
                                       ccGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGaa                                                                                                                <  2:1530064/150‑1 (MQ=255)
                                        cGGACGTCGGCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGGTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAAc                                                                                                               <  2:228380/150‑1 (MQ=255)
                                          gacgacGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCt                                                                                                             >  2:496965/1‑150 (MQ=255)
                                           acgacgTCCTATTTTCGGGCAATCGTGCAACTCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGATGGGCTGCGCCGGTTGAACAtg                                                                                                            >  1:1263273/1‑150 (MQ=255)
                                               cgTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGAc                                                                                                        <  1:1742467/150‑1 (MQ=255)
                                                  ccTATTTTCGGGCAATCGTGCATCGCAGCCAGAATATGACGAATACGATCGATTATTAAACGGTGCGCAAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACTAAGCTGGGCTCCGCCTGGTGAACCTGTGACTCa                                                                                                     >  2:384789/1‑150 (MQ=255)
                                                   cTATTTTCGGGCAGTCGTGCAACGCAGCCAGAATATGACGAATACGATGCAGTATTAAAAGGTGGGACAATTACCGAACCTGTCGCTGTAGAAGCAGCAGCTACCACGGCGACACAAAGCTGGGCTGCGAAGGTTGACCCTGTGACACAg                                                                                                    >  2:166557/1‑150 (MQ=255)
                                                      ttttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcg                                                                                                 >  2:533741/1‑150 (MQ=255)
                                                        ttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcgcc                                                                                               <  2:1589570/150‑1 (MQ=255)
                                                        ttCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGAcgcc                                                                                               >  1:657965/1‑150 (MQ=255)
                                                            ggCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCt                                                                                           >  2:473976/1‑150 (MQ=255)
                                                              cAATCGTGCAACGCAGCCAGAATATGACGAATACGAGCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCGGGGCTGCGCCGGTTGAACCTGTGCCTCAGACGCCGCCTGt                                                                                         >  1:905290/1‑150 (MQ=255)
                                                               aaTCGTGCAACGCATCAATAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGtt                                                                                        <  1:554952/150‑1 (MQ=255)
                                                                  cGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGCACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCCGACGCCGCCTGTTGcc                                                                                     >  1:1139508/1‑150 (MQ=255)
                                                                   gTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCct                                                                                    >  1:469656/1‑150 (MQ=255)
                                                                      cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTTCTACCACGGCGACACAAAGCTGGGCTGCGCCGTTTGAACCTGTGACTCAGACGCCGCCTGGTGCCGCTg                                                                                 >  2:329867/1‑150 (MQ=255)
                                                                      cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACgccgcc                                                                                            <  1:1307477/139‑1 (MQ=255)
                                                                      cAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACgccgcc                                                                                            >  2:1307477/1‑139 (MQ=255)
                                                                        aCGCAGCCAGAATATGAAGAATCCTATCCATTATTCAACGGTGCGCCACTTACCGCACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGtt                                                                               <  2:916425/150‑1 (MQ=255)
                                                                          gCAGCCAGAATATGACGAATAAGATACATTATTAAACGGTGCGAAAATTACTTAACATGTCTCTGTAGCAGCTGCTGATACCACGGCTACAAAAAGATGGGCTGAGCCGGTCTCACCTGTGACTAAGACGCCGCCTGTTTCATCTGtgta                                                                             >  1:691831/1‑147 (MQ=255)
                                                                          gCAGACAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGa                                                                             <  1:1232610/150‑1 (MQ=255)
                                                                            aGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATg                                                                           <  1:1735973/150‑1 (MQ=255)
                                                                            aGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATg                                                                           <  2:602210/150‑1 (MQ=255)
                                                                                   atatGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTTATGTTCCAcc                                                                    >  1:1710962/1‑150 (MQ=255)
                                                                                   atatGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCAcc                                                                    <  2:1743603/150‑1 (MQ=255)
                                                                                        aCGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTgcgc                                                               <  1:1407308/150‑1 (MQ=255)
                                                                                               cGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTAc                                                        >  2:1241149/1‑150 (MQ=255)
                                                                                               cGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTAc                                                        >  2:1045551/1‑150 (MQ=255)
                                                                                                 agccATTATTAACCGGTGCGCCAATTACCGAACCTGGCGCTGTAGCAGCTGCGGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                      <  1:455605/148‑1 (MQ=255)
                                                                                                 aTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                      >  2:1759266/1‑150 (MQ=255)
                                                                                                 aTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                      <  1:1454608/150‑1 (MQ=255)
                                                                                                 aTCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAg                                                      >  1:1102830/1‑150 (MQ=255)
                                                                                                     attattAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGc                                                  <  2:1769294/150‑1 (MQ=255)
                                                                                                      ttattaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGCTGTTCCACCTGCGCAACCTACAGTAGcc                                                 >  1:1217054/1‑150 (MQ=255)
                                                                                                        attaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTg                                               <  1:617979/150‑1 (MQ=255)
                                                                                                        attaAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCAAGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCGTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTg                                               >  2:717823/1‑150 (MQ=255)
                                                                                                         ttaAACGGTGCGCCAATTACCGAACGTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTgg                                              <  1:97008/150‑1 (MQ=255)
                                                                                                           aaaCGGTGCGCCAATTACAGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCTCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCAggaa                                            >  1:193972/1‑148 (MQ=255)
                                                                                                            aaCGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCCCCTGCGCAACCTACAGTAGCCTGGCAg                                           >  1:1818755/1‑150 (MQ=255)
                                                                                                            aaCGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAg                                           <  1:803427/150‑1 (MQ=255)
                                                                                                             ccGGTGCGCGAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGc                                          <  2:186998/149‑1 (MQ=255)
                                                                                                               ggTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACTAATCTGGGCTGCGCCGGATGAACCTGTGACTCATACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCt                                        >  1:144731/1‑150 (MQ=255)
                                                                                                                gTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTg                                       <  1:719190/150‑1 (MQ=255)
                                                                                                                gTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTg                                       <  2:1734912/150‑1 (MQ=255)
                                                                                                                 tGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGt                                      >  2:412846/1‑150 (MQ=255)
                                                                                                                    gcCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTAcc                                   >  2:690667/1‑150 (MQ=255)
                                                                                                                      cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGCACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCgg                                 >  2:227672/1‑150 (MQ=255)
                                                                                                                      cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCACCCTACAGTAGCCTGGCAGCCTGTACCgg                                 >  2:907385/1‑150 (MQ=255)
                                                                                                                      cAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCgg                                 >  1:1074032/1‑150 (MQ=255)
                                                                                                                       aaTTACCCAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGTCACAAAGCTGGGCGGCGCCGGTTGAACCTGGGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCggg                                <  2:1383394/150‑1 (MQ=255)
                                                                                                                        aTTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGt                               <  1:313276/150‑1 (MQ=255)
                                                                                                                          tACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTcc                             <  1:3617/150‑1 (MQ=255)
                                                                                                                           aCCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGCGTccc                            >  1:85835/1‑149 (MQ=255)
                                                                                                                            ccGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGCACGTGTGGCGCAGACGCCGCCGGTTGCCGCTGTTGATGTTCCACCTGCGCCACCGACAGTAGCCTGGCAGCCTGTCCCGGGTcgcc                           >  2:319902/1‑147 (MQ=255)
                                                                                                                            ccGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCcac                           >  2:1638161/1‑150 (MQ=255)
                                                                                                                            agctttgtGTCGCCGTGGTAGCAGCAGCTGCTACAGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGGACCCTACAGTCGCCTGGCGGCGTGTACCGGGTCcac                           >  1:1300086/8‑150 (MQ=25)
                                                                                                                                aCCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAAc                       <  1:353609/150‑1 (MQ=255)
                                                                                                                                   tggcGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACggg                    <  2:596494/147‑1 (MQ=255)
                                                                                                                                   gggcgcgGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACggg                    <  1:1430157/147‑1 (MQ=255)
                                                                                                                                   ggTCGCGGTCCCAGCTGCGGCTACCACGGCGACACAAAGCGGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCGCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACtgg                    <  2:170477/149‑1 (MQ=255)
                                                                                                                                      cGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGgagc                 >  1:1042249/1‑149 (MQ=255)
                                                                                                                                        cTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCAGGTTGAACCTGTGACTCAGACGCCGCCTGTTGGCTCTGTTGATGTTCCACCTGCGCAACCTACTGTAGCCTGGCAGCCTGTCCCGGGTCCACAAACGGGAGAGc               >  2:1166795/1‑150 (MQ=255)
                                                                                                                                               agcTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTAt        <  2:1747203/150‑1 (MQ=255)
                                                                                                                                                 gtgctgctACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACTGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTg      <  2:1180282/149‑1 (MQ=255)
                                                                                                                                                   gctgctACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGCt    >  2:818671/1‑150 (MQ=255)
                                                                                                                                                     tgctACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGCTcc  >  2:668923/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAGAGATTACCTACACTGCACGCGGTGTGGCTGCCGACCCGGACGACGTCCTATTTTCGGGCAATCGTGCAACGCAGCCAGAATATGACGAATACGATCCATTATTAAACGGTGCGCCAATTACCGAACCTGTCGCTGTAGCAGCTGCTGCTACCACGGCGACACAAAGCTGGGCTGCGCCGGTTGAACCTGTGACTCAGACGCCGCCTGTTGCCTCTGTTGATGTTCCACCTGCGCAACCTACAGTAGCCTGGCAGCCTGTACCGGGTCCACAAACGGGAGAGCCGGTTATTGCTCC  >  NC_000913/934049‑934347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: