BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	940,294	A→T	5.6%	K289N (AAA→AAT)	serS →	seryl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	940,294	0	A	T	5.6%	386.6 / 7.8	131	K289N (AAA→AAT)	serS	seryl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (64/59); new base T (4/4); total (68/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 135 total aligned reads displayed.

GTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGC  >  NC_000913/940145‑940441

gTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCCCACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGTCACCCGTGGTCTGATCCGTATGCACCAGTTCGACaaa                                                                                                                                                     >  2:876750/1‑150 (MQ=255)

          cTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATgg                                                                                                                                           >  1:1184265/1‑150 (MQ=255)

            aaCCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTg                                                                                                                                         >  1:120334/1‑150 (MQ=255)

             cccTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCATTTCAACTTTGTCGAACTGGTGc                                                                                                                                        <  2:1008335/149‑1 (MQ=255)

                   tGCGCGGTGAAATCTTCGATGATGATGATCTGCCAATTAAGATGACCGCCCACACCGCATGCTTCCGTTCTGATGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCATTTCAACTTTGTCGAACTGGTGCatacgg                                                                                                                                  <  1:397303/150‑6 (MQ=37)

                   tGCGCGGTGAAATCATCGATGAAGATGATCTGCAAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGAAACACGTGGTCTGCTCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCg                                                                                                                                  >  1:273857/1‑150 (MQ=255)

                    gcgcGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGt                                                                                                                                 >  1:994373/1‑150 (MQ=255)

                     cgcgGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACCCCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTg                                                                                                                                <  1:1225925/150‑1 (MQ=255)

                      gcgGTGAACTCATCGATGAAGATGAGCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAACCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTgc                                                                                                                               <  2:1578837/150‑1 (MQ=255)

                       cgGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTgcg                                                                                                                              <  1:1399779/150‑1 (MQ=255)

                          tGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACGCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGccc                                                                                                                           <  1:124049/150‑1 (MQ=255)

                              atcctcGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGATCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCagaa                                                                                                                       <  2:1487616/150‑1 (MQ=255)

                                 atcGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGAc                                                                                                                    <  1:634469/150‑1 (MQ=255)

                                 atcGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGAc                                                                                                                    <  1:1414569/150‑1 (MQ=255)

                                   cGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTc                                                                                                                  >  2:1603071/1‑150 (MQ=255)

                                   cGATGAAGATGAGCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCAGCAGTTCGACAAAGTTGAAATGGGGCAGATCGTGCGCACAGAAGACTc                                                                                                                  >  2:56362/1‑150 (MQ=255)

                                    gATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCTTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCTACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCa                                                                                                                 <  2:1502433/150‑1 (MQ=255)

                                      tGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAAt                                                                                                               <  1:1360880/150‑1 (MQ=255)

                                       gAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATg                                                                                                              >  2:549325/1‑150 (MQ=255)

                                          gatgatCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATggcg                                                                                                           >  1:1456849/1‑150 (MQ=255)

                                            tgatCTGCCAATTAAGATGACCGCCCACCCCCCATGCTTCCGTTCTGCAGCCGGTTCATATGGTCGTGCCACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATggcggc                                                                                                         >  2:1292685/1‑150 (MQ=255)

                                             gatCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGCCAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGgcggcg                                                                                                        >  2:1695763/1‑150 (MQ=255)

                                                cTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTg                                                                                                     <  1:741394/150‑1 (MQ=255)

                                                cTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTg                                                                                                     <  2:32018/150‑1 (MQ=255)

                                                  gCCAATTCAGATGACCGCCGACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGTTCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGa                                                                                                   <  2:1690998/150‑1 (MQ=255)

                                                  gCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGa                                                                                                   >  1:1568373/1‑150 (MQ=255)

                                                  gCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGa                                                                                                   <  2:701812/150‑1 (MQ=255)

                                                   ccAATTCATATTCCCTCCCAACCCCCATGCTAACGTTCTGAAGCCGGTTCATATCTGCGCGACACCAGTCTTATGATCCGTCTGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCTCCCAGAAGACTCACTGGCGGCGCTGGaa                                                                                                  <  2:112490/150‑1 (MQ=255)

                                                    cAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAag                                                                                                 <  1:419803/150‑1 (MQ=255)

                                                      aTTAAGATGACCGCCCACACCCCATGCGTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAagag                                                                                               <  1:1159193/150‑1 (MQ=255)

                                                        tAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGAt                                                                                             <  2:775639/150‑1 (MQ=255)

                                                         aaGATGACCGCCCACACCCCTTGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATg                                                                                            <  1:167412/150‑1 (MQ=255)

                                                         aaGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACCCCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATg                                                                                            >  2:1683638/1‑150 (MQ=255)

                                                         aaGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATg                                                                                            <  1:686337/150‑1 (MQ=255)

                                                           gATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGAc                                                                                          >  1:1717434/1‑150 (MQ=255)

                                                           gATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGAc                                                                                          >  1:684083/1‑150 (MQ=255)

                                                            aTGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACt                                                                                         >  1:513232/1‑150 (MQ=255)

                                                                ccGCCCACACCCCCTGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTc                                                                                     <  2:9016/150‑1 (MQ=255)

                                                                ccGCCCACACCCCATGCGTCCGTTCTGAAGCCGGTTCATATGGGCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTc                                                                                     <  2:1755208/150‑1 (MQ=255)

                                                                ccGCCAACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACACGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCGAGAAGACTCAATGGCGGCGCTGGTAGAGATGACTGGTc                                                                                     >  1:148543/1‑150 (MQ=255)

                                                                 cGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCa                                                                                    <  2:1666578/150‑1 (MQ=255)

                                                                  gCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAATTGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCAt                                                                                   >  1:1734836/1‑150 (MQ=255)

                                                                  gCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGAATGGTCAt                                                                                   >  2:48328/1‑150 (MQ=255)

                                                                    ccACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGAAAAATTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCGGGAAGAGATGACTGGTCATGc                                                                                 >  1:1020577/1‑150 (MQ=255)

                                                                     cacaCCCCATGCTTCCGGTCTGAAGCCGGGTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCa                                                                                <  2:769043/150‑1 (MQ=255)

                                                                         ccccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAag                                                                                                 >  1:417455/1‑129 (MQ=255)

                                                                         ccccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAag                                                                                                 <  2:417455/129‑1 (MQ=255)

                                                                         ccccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGaaa                                                                            >  1:1556792/1‑150 (MQ=255)

                                                                         ccccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGaaa                                                                            >  2:1645532/1‑150 (MQ=255)

                                                                           ccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGaaaaa                                                                          >  2:1249712/1‑150 (MQ=255)

                                                                           ccATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGaaaaa                                                                          >  1:1490636/1‑150 (MQ=255)

                                                                             aTGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGt                                                                        <  1:1762833/150‑1 (MQ=255)

                                                                                cTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACTCCCGGGGTCTGATCCGTATGCCCCAGGTCGACAAAGTTGCAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCt                                                                     >  2:1761550/1‑150 (MQ=255)

                                                                                 ttCCGTTCTGACGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTg                                                                    <  2:1552542/150‑1 (MQ=255)

                                                                                 ttCCGTTCTGAAGCCTGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTg                                                                    <  1:853179/150‑1 (MQ=255)

                                                                                   ccGTTCTGAAGCCGGTTCATGTGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCa                                                                  <  1:30463/150‑1 (MQ=255)

                                                                                   ccGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGCTCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCa                                                                  >  2:572601/1‑150 (MQ=255)

                                                                                          gAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTg                                                           <  1:469582/150‑1 (MQ=255)

                                                                                           aaGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGCCTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTgg                                                          >  2:1186821/1‑150 (MQ=255)

                                                                                            aGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGATTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg                                                         >  1:1502517/1‑150 (MQ=255)

                                                                                            aGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg                                                         <  2:261210/150‑1 (MQ=255)

                                                                                               cGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCt                                                      >  2:455420/1‑150 (MQ=255)

                                                                                                  ttCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGTAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGcc                                                   <  2:1450013/150‑1 (MQ=255)

                                                                                                  ttCATATGGGCGGGACACCCGTGGTCTGATCCGTATGCACCAGTGCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGCCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGcc                                                   <  2:537099/150‑1 (MQ=255)

                                                                                                    cATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGATGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgt                                                 >  1:1057319/1‑150 (MQ=255)

                                                                                                    cATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgt                                                 >  1:1049931/1‑150 (MQ=255)

                                                                                                      tatGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtac                                               >  1:947000/1‑150 (MQ=255)

                                                                                                          gacGTGACAGCCGTTGGCTGATCCGAATACCCCAGTTCGACACAGTTGAAAGGTTGCAGATCGTGCGCACAGACGACTCAAAGGCGTCGCTGGAACCGATGACTGGTCATGCAGAAAAATTCCTGCAGTTGCTGGGCCTTccgtaccgta                                           <  1:282357/148‑1 (MQ=255)

                                                                                                              tGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAat                                       <  2:861931/150‑1 (MQ=255)

                                                                                                               gACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTTCTGGGCCTGCCGTACCGTAAAatc                                      >  1:9258/1‑150 (MQ=255)

                                                                                                               gACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCATGCCGTACCGTAAAata                                      >  1:859222/1‑149 (MQ=255)

                                                                                                                 cacCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatcat                                    >  1:83434/1‑150 (MQ=255)

                                                                                                                 cacCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatcaa                                    >  1:629759/1‑149 (MQ=255)

                                                                                                                 cacCCGTGGTCTGAGCCGTATGCACCCGTTCGACAAAGTTGAACTGGTGCAGATCGGGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatcat                                    <  2:1402327/150‑1 (MQ=255)

                                                                                                                  acCCGTCGTCTGATCCGTATGCACCAGTTAGACAAAGTTGAAATGGTGCAGATCGAGCGCCCAGAAGCCTCAATGGCGGCGCTGGAAGAGATCACTCGTCATGAAGCAAAAGTCCTGCAGTAGCTGGGCCTGCCGTACCGTAAAatcatc                                   >  1:207190/1‑150 (MQ=255)

                                                                                                                           cTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACt                          >  2:1455294/1‑150 (MQ=255)

                                                                                                                           cTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACt                          >  1:1852947/1‑150 (MQ=255)

                                                                                                                           cTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACt                          >  2:890812/1‑150 (MQ=255)

                                                                                                                             gATCCGTATGCACCAGTTCTACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTgg                        <  1:584844/150‑1 (MQ=255)

                                                                                                                             gATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTgg                        >  1:312168/1‑150 (MQ=255)

                                                                                                                              cacgatctGCACCATTTCAACTTTGTCGAACTGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGc                       >  1:48551/8‑150 (MQ=38)

                                                                                                                                cgatctGCACCATTTCAACTTTGTCGAACTGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGa                     >  1:14568/6‑150 (MQ=38)

                                                                                                                                 cGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc                    <  2:1717434/150‑1 (MQ=255)

                                                                                                                                 cGTATGCACCAGTTCGACAAAGTAGAAATGGTGCAGCTCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGATGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTCCCGTACAGTAAAATCATCCTTTGCACTGGCGAc                    >  2:905586/1‑150 (MQ=255)

                                                                                                                                  ttcagCACCAGTTCGACAAATTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATTGCGGCGCTGGAAGATATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTTCCGTACCGTAAAATCATCCTTTGCACTGGCGACa                   <  1:367277/146‑1 (MQ=255)

                                                                                                                                  atctGCACCATTTCAACTTTGTCGAACTGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACa                   >  1:1442058/4‑150 (MQ=255)

                                                                                                                                     tGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATgg                >  2:1313119/1‑150 (MQ=255)

                                                                                                                                      gCAACAGTTCGACAAAGTTGAAATGTTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATggg               <  1:1557553/150‑1 (MQ=255)

                                                                                                                                       cACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTTGGCCTGCCGTACCGTAAAATCATCCTATGCACTGGCGACATGGGc              >  2:425004/1‑150 (MQ=255)

                                                                                                                                          cAGTTCGACACAGGTGCAATGGGCCCGATCGTGCGCCCACAACACTCAATCGCGGCGCTGGAAGACCTGACTTGTCATGCAGAACAAGCCCTGCAGTCGCCGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGTCttt           <  2:330179/150‑1 (MQ=255)

                                                                                                                                           aGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTg          >  2:1087753/1‑150 (MQ=255)

                                                                                                                                              tCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGATGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGGAAAATCATCCTTTGCTCTGGCGACATGGGCTTTGgcg       >  1:235949/1‑150 (MQ=255)

                                                                                                                                               cGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGgcgc      >  2:1759747/1‑150 (MQ=255)

                                                                                                                                               cGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGgcgc      >  2:579706/1‑150 (MQ=255)

                                                                                                                                               cGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGgcgc      >  1:1653094/1‑150 (MQ=255)

                                                                                                                                                gACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCt     >  2:583598/1‑150 (MQ=255)

                                                                                                                                                  cAAAGTTGACATGGTGCAGATCGTCCGCCCAGAAGACTCCACGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAATGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAATATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTg   <  2:203090/150‑1 (MQ=255)

                                                                                                                                                  cAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTg   >  1:1162330/1‑150 (MQ=255)

                                                                                                                                                   aaaGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGc  >  2:1500176/1‑150 (MQ=255)

                                                                                                                                                   aaaCTTGAAATGGTGCAGATCGGGCGCCCAGAAGACGCAATGCCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCGGCAGGTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGc  <  2:1173092/150‑1 (MQ=255)

GTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAAGATGACCGCCCACACCCCATGCTTCCGTTCTGAAGCCGGTTCATATGGTCGTGACACCCGTGGTCTGATCCGTATGCACCAGTTCGACAAAGTTGAAATGGTGCAGATCGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGC  >  NC_000913/940145‑940441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`