Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,254 A→G 5.3% intergenic (+20/+23) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2540AG 5.3% 288.6 / 5.1 95intergenic (+20/+23)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (39/51);  new base G (2/3);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

ACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTC  >  NC_000913/993105‑993400
                                                                                                                                                     |                                                                                                                                                  
aCCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCa                                                                                                                                                    >  2:531755/1‑150 (MQ=255)
          cGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgccgc                                                                                                                                          >  1:595340/1‑150 (MQ=255)
          cGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgccgc                                                                                                                                          <  2:1272128/150‑1 (MQ=255)
           gCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCgccgcc                                                                                                                                         >  2:1633504/1‑150 (MQ=255)
            ccTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCa                                                                                                                                        >  2:377897/1‑150 (MQ=255)
             cTGAAACGTTACGATGCCAAACGTCAGGAGATAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCTGCGCTATTGCCGCCAt                                                                                                                                       <  1:1745792/150‑1 (MQ=255)
              tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAtt                                                                                                                                      <  1:783990/150‑1 (MQ=255)
              tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAtt                                                                                                                                      <  1:666149/150‑1 (MQ=255)
               gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCTGCGCGGGAACAGTAGCAAGGGCTGGAAAATCTCTCTGGCGCGCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCACTAGCGCCGCCATTc                                                                                                                                     <  1:267740/150‑1 (MQ=255)
               gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                     <  1:750031/150‑1 (MQ=255)
                aaaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCg                                                                                                                                    >  1:1465233/1‑150 (MQ=255)
                  aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                  <  1:1275358/150‑1 (MQ=255)
                  aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                  <  1:741984/150‑1 (MQ=255)
                  aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                  <  1:543179/150‑1 (MQ=255)
                   cGTTACGATGACAAACGTCAGCAGAAACTGCGCGCGGCGCTGGAACAGTTGAAAGGGCGGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgggg                                                                                                                                 <  1:247699/150‑1 (MQ=255)
                      tACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAt                                                                                                                              <  1:826050/150‑1 (MQ=255)
                       aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                             <  2:1800245/150‑1 (MQ=255)
                        cGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACTGTTGAAAGGGCTGGAATATCTCTCTGGCGATCTGTACGAGATGATAACTAAAGCACTGGCTTGATATATAACCGAATGGCGGCAATAGCACCGCCATTCGGGGAAttt                                                                                                                            >  2:752581/1‑150 (MQ=255)
                        cGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAttt                                                                                                                            >  1:480385/1‑150 (MQ=255)
                          aTGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAATATCTCTCTGGCGATCTGTCCGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAc                                                                                                                          >  1:743470/1‑150 (MQ=255)
                           tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                         >  1:181050/1‑150 (MQ=255)
                           tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                         <  1:1476415/150‑1 (MQ=255)
                           tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAAGCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                         <  1:808884/150‑1 (MQ=255)
                             ccAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcccc                                                                                                                       >  2:12061/1‑150 (MQ=255)
                             ccAAACGTCAGGAGAAAATCCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcccc                                                                                                                       <  2:334899/150‑1 (MQ=255)
                                aaCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGt                                                                                                                    >  1:17780/1‑150 (MQ=255)
                                      aGGAGAAAATGAGCGCGGCGCTGGAACAGTTGATAGTGATGTAAAATCTCTCTGGCGAGCTGTACGAGAAGATACCTACAGCACTGGCTTGATAATTAACCGAATGGCGTCAATAGCGCCGCCATTCTGGGAATTTACCCCTGTTTtctc                                                                                                              <  1:287249/150‑1 (MQ=255)
                                       ggAGAAAATGCGCGCGGCGCTGGAACAGTTTAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGCGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                             <  2:286782/150‑1 (MQ=255)
                                         agaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAgg                                                                                                           <  1:619337/150‑1 (MQ=255)
                                          gaAAATGCGCGCCGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATACCTAAAGCACTCGCTTGATCAATAACCGAATGGCGGCAATAGGGCCGCGATTGGGGGAGTTTACCCCTGTTTtctcgtgc                                                                                                          >  2:116945/1‑146 (MQ=255)
                                           ataaTGCCCGCGGCGCTGGAACTGTTGAAAGGGCTGTAATATCTCTCTGGCGATCTGGACGAGAAGATAACAAAAGCACTGGCGTGATAAATAACCGAATGGCGGCAATAGCGCCGGCATTCGGGGAATTTACCCCTGGTTTCTCAGGCg                                                                                                         <  2:1163402/148‑1 (MQ=255)
                                             aaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                       <  1:1556142/150‑1 (MQ=255)
                                               tGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGtt                                                                                                     <  1:1633504/150‑1 (MQ=255)
                                                 cgcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATGTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTc                                                                                                   <  2:338631/150‑1 (MQ=255)
                                                    gcggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGTATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGa                                                                                                <  2:916164/150‑1 (MQ=255)
                                                        cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCAATGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                            >  1:310796/1‑150 (MQ=255)
                                                          cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                          >  2:1087977/1‑150 (MQ=255)
                                                          cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                          <  1:125032/150‑1 (MQ=255)
                                                            ggAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcaccac                                                                                        >  2:1789351/1‑150 (MQ=255)
                                                             gAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACg                                                                                       >  2:473060/1‑150 (MQ=255)
                                                                cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                    <  1:1772334/150‑1 (MQ=255)
                                                                 aGTTGAAAGGGCTGGATAATCTCTCTCGCGATCTGTACGAGAAGATAACTAAAGCTCTGGCTTTATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                   <  2:678575/150‑1 (MQ=255)
                                                                 aGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                   <  1:1033968/150‑1 (MQ=255)
                                                                  gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                  <  2:502605/150‑1 (MQ=255)
                                                                  gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                  >  1:528903/1‑150 (MQ=255)
                                                                    tGAAAGGTCTGGAAAATCTCGCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATCCCCGTCTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATCCACCACGTTGCATc                                                                                <  2:45252/150‑1 (MQ=255)
                                                                    tGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATc                                                                                <  1:1838474/150‑1 (MQ=255)
                                                                      aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAc                                                                              <  2:61257/150‑1 (MQ=255)
                                                                       aaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAcc                                                                             >  1:464232/1‑150 (MQ=255)
                                                                           gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                         >  1:1698788/1‑150 (MQ=255)
                                                                             tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                       >  2:265189/1‑150 (MQ=255)
                                                                                aaaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAac                                                                    <  2:134128/150‑1 (MQ=255)
                                                                                      tctctGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                              <  1:746321/150‑1 (MQ=255)
                                                                                       ctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCg                                                             >  2:1604560/1‑150 (MQ=255)
                                                                                         ctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCCACACCTCCGCt                                                           >  2:750203/1‑150 (MQ=255)
                                                                                             ctaTCTGTACGATAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCACTAGCGCAGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       <  1:1809277/148‑1 (MQ=255)
                                                                                             cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       <  1:670794/150‑1 (MQ=255)
                                                                                             cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       <  1:1612694/150‑1 (MQ=255)
                                                                                             cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       <  2:40298/150‑1 (MQ=255)
                                                                                             cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       <  2:1709127/150‑1 (MQ=255)
                                                                                                   gTACGAGAAGATAACTAAATCAATGGCTTGATAAATAACCGAATGGTGGCAATAGCTCCGCCATTCTGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCg                                                 <  2:1069492/150‑1 (MQ=255)
                                                                                                    tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                <  2:863915/150‑1 (MQ=255)
                                                                                                    tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCCTCCAGCTCCGc                                                <  1:87017/150‑1 (MQ=255)
                                                                                                      cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCTTTGCAACACCTCCGCTTCCCGCTCCGATa                                              >  2:349785/1‑150 (MQ=255)
                                                                                                      cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                              >  2:373194/1‑150 (MQ=255)
                                                                                                         taagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                           <  1:1582713/149‑1 (MQ=255)
                                                                                                         gaagaTAACTAAAGCTCTGGCTTTATAAATAACAGAATTGCGGCAATTGCGCCGCAATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCTATTCCAGCTCCGCTAATc                                           <  2:797186/150‑1 (MQ=255)
                                                                                                         gaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                           <  2:1539524/150‑1 (MQ=255)
                                                                                                            taTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCa                                        <  2:244567/149‑1 (MQ=255)
                                                                                                               aaCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCATGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACAACCGCTTCCAGCTCCGCTAATCGccccc                                     >  1:1286131/1‑149 (MQ=255)
                                                                                                                aCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGa                                    >  1:378374/1‑150 (MQ=255)
                                                                                                                aCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCGGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGATTCCAGCTCCGCTAATCGCACCgc                                    >  2:1153497/1‑149 (MQ=255)
                                                                                                                 cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTCCCCCTGTTTTCTCAGGCGCGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAAGGGCCCCGAc                                   >  2:1659119/1‑150 (MQ=255)
                                                                                                                 cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAc                                   <  2:832664/150‑1 (MQ=255)
                                                                                                                 cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAAAGAGCCGCAATTCGGCGAATTTACCCCTGTTATCTCAGCCGAGTTTCAGAATAACCACGTTGCAACACACGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcagcc                                   >  1:1352810/1‑146 (MQ=255)
                                                                                                                           ggCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                         <  1:1235858/150‑1 (MQ=255)
                                                                                                                            gCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcg                        <  2:1465233/150‑1 (MQ=255)
                                                                                                                             cTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcgc                       >  2:1766970/1‑150 (MQ=255)
                                                                                                                              ttGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcgcg                      <  1:1423456/150‑1 (MQ=255)
                                                                                                                                  taaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCTCCGCCCCTAATCGACGCGGACg                  >  1:1209017/1‑150 (MQ=255)
                                                                                                                                   aaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGCCGCGGACgg                 >  2:1795982/1‑150 (MQ=255)
                                                                                                                                    aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                >  2:1849830/1‑150 (MQ=255)
                                                                                                                                    aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                <  1:373194/150‑1 (MQ=255)
                                                                                                                                    aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                >  2:131796/1‑150 (MQ=255)
                                                                                                                                    aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTAACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCCCCGACCCTAATCGACGCGGACggg                >  1:1864889/1‑150 (MQ=255)
                                                                                                                                      taaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggg              <  1:377897/150‑1 (MQ=255)
                                                                                                                                       aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGGTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAACGCCACTAATCGACGCGGACgggggg             >  1:455076/1‑149 (MQ=255)
                                                                                                                                        cccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCTCTAATCGCACAGACCCTAATCGACGCGGCCGGGGGat            >  2:73256/2‑150 (MQ=255)
                                                                                                                                        cccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggggt            >  1:1869017/2‑148 (MQ=255)
                                                                                                                                          cGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCCCCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGatat          >  2:1407293/1‑150 (MQ=255)
                                                                                                                                           gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCGGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc         <  2:921050/150‑1 (MQ=255)
                                                                                                                                            aaTGGCGTCATTATCTCCTCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCCAGTTTCAGAGTAGCCACCTTGCCTCCCCCGTTGCACCACCTCCGCTTCCAGCACCGCTATACGCAGCGACCATAATCGACGCGCACGGGGGCTAGcc        >  2:1136238/1‑150 (MQ=255)
                                                                                                                                                gcggcAATCGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGGTTCAGATTCACCACGTAGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGTGGATATCCACTg    <  1:1207425/150‑1 (MQ=255)
                                                                                                                                                gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTg    <  2:17780/150‑1 (MQ=255)
                                                                                                                                                  ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGCTACTCGCACCGACCCTACTCGCCGCGGACGGGGGATATCCACGGTc  >  1:496992/1‑150 (MQ=255)
                                                                                                                                                  ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGCCGGGGGATATCCACTGTc  >  1:1678602/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTC  >  NC_000913/993105‑993400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: