Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,255 A→C 6.8% intergenic (+21/+22) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2550AC 6.8% 271.8 / 6.3 95intergenic (+21/+22)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (38/49);  new base C (2/5);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAAT  >  NC_000913/993115‑993404
                                                                                                                                            |                                                                                                                                                     
cGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgccgc                                                                                                                                              <  2:1272128/150‑1 (MQ=255)
cGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgccgc                                                                                                                                              >  1:595340/1‑150 (MQ=255)
 gCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCgccgcc                                                                                                                                             >  2:1633504/1‑150 (MQ=255)
  ccTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCa                                                                                                                                            >  2:377897/1‑150 (MQ=255)
   cTGAAACGTTACGATGCCAAACGTCAGGAGATAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCTGCGCTATTGCCGCCAt                                                                                                                                           <  1:1745792/150‑1 (MQ=255)
    tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAtt                                                                                                                                          <  1:783990/150‑1 (MQ=255)
    tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAtt                                                                                                                                          <  1:666149/150‑1 (MQ=255)
     gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCTGCGCGGGAACAGTAGCAAGGGCTGGAAAATCTCTCTGGCGCGCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCACTAGCGCCGCCATTc                                                                                                                                         <  1:267740/150‑1 (MQ=255)
     gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                         <  1:750031/150‑1 (MQ=255)
      aaaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCg                                                                                                                                        >  1:1465233/1‑150 (MQ=255)
        aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                      <  1:543179/150‑1 (MQ=255)
        aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                      <  1:741984/150‑1 (MQ=255)
        aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                      <  1:1275358/150‑1 (MQ=255)
         cGTTACGATGACAAACGTCAGCAGAAACTGCGCGCGGCGCTGGAACAGTTGAAAGGGCGGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgggg                                                                                                                                     <  1:247699/150‑1 (MQ=255)
            tACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAt                                                                                                                                  <  1:826050/150‑1 (MQ=255)
             aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                                 <  2:1800245/150‑1 (MQ=255)
              cGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACTGTTGAAAGGGCTGGAATATCTCTCTGGCGATCTGTACGAGATGATAACTAAAGCACTGGCTTGATATATAACCGAATGGCGGCAATAGCACCGCCATTCGGGGAAttt                                                                                                                                >  2:752581/1‑150 (MQ=255)
              cGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAttt                                                                                                                                >  1:480385/1‑150 (MQ=255)
                aTGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAATATCTCTCTGGCGATCTGTCCGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAc                                                                                                                              >  1:743470/1‑150 (MQ=255)
                 tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                             >  1:181050/1‑150 (MQ=255)
                 tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                             <  1:1476415/150‑1 (MQ=255)
                 tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAAGCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                             <  1:808884/150‑1 (MQ=255)
                   ccAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcccc                                                                                                                           >  2:12061/1‑150 (MQ=255)
                   ccAAACGTCAGGAGAAAATCCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcccc                                                                                                                           <  2:334899/150‑1 (MQ=255)
                      aaCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGt                                                                                                                        >  1:17780/1‑150 (MQ=255)
                            aGGAGAAAATGAGCGCGGCGCTGGAACAGTTGATAGTGATGTAAAATCTCTCTGGCGAGCTGTACGAGAAGATACCTACAGCACTGGCTTGATAATTAACCGAATGGCGTCAATAGCGCCGCCATTCTGGGAATTTACCCCTGTTTtctc                                                                                                                  <  1:287249/150‑1 (MQ=255)
                             ggAGAAAATGCGCGCGGCGCTGGAACAGTTTAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGCGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                 <  2:286782/150‑1 (MQ=255)
                               agaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAgg                                                                                                               <  1:619337/150‑1 (MQ=255)
                                gaAAATGCGCGCCGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATACCTAAAGCACTCGCTTGATCAATAACCGAATGGCGGCAATAGGGCCGCGATTGGGGGAGTTTACCCCTGTTTtctcgtgc                                                                                                              >  2:116945/1‑146 (MQ=255)
                                 ataaTGCCCGCGGCGCTGGAACTGTTGAAAGGGCTGTAATATCTCTCTGGCGATCTGGACGAGAAGATAACAAAAGCACTGGCGTGATAAATAACCGAATGGCGGCAATAGCGCCGGCATTCGGGGAATTTACCCCTGGTTTCTCAGGCg                                                                                                             <  2:1163402/148‑1 (MQ=255)
                                   aaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                           <  1:1556142/150‑1 (MQ=255)
                                     tGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGtt                                                                                                         <  1:1633504/150‑1 (MQ=255)
                                       cgcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATGTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTc                                                                                                       <  2:338631/150‑1 (MQ=255)
                                          gcggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGTATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGa                                                                                                    <  2:916164/150‑1 (MQ=255)
                                              cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCAATGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                >  1:310796/1‑150 (MQ=255)
                                                cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                              >  2:1087977/1‑150 (MQ=255)
                                                cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                              <  1:125032/150‑1 (MQ=255)
                                                  ggAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcaccac                                                                                            >  2:1789351/1‑150 (MQ=255)
                                                   gAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACg                                                                                           >  2:473060/1‑150 (MQ=255)
                                                      cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                        <  1:1772334/150‑1 (MQ=255)
                                                       aGTTGAAAGGGCTGGATAATCTCTCTCGCGATCTGTACGAGAAGATAACTAAAGCTCTGGCTTTATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                       <  2:678575/150‑1 (MQ=255)
                                                       aGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                       <  1:1033968/150‑1 (MQ=255)
                                                        gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                      >  1:528903/1‑150 (MQ=255)
                                                        gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                      <  2:502605/150‑1 (MQ=255)
                                                          tGAAAGGTCTGGAAAATCTCGCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATCCCCGTCTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATCCACCACGTTGCATc                                                                                    <  2:45252/150‑1 (MQ=255)
                                                          tGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATc                                                                                    <  1:1838474/150‑1 (MQ=255)
                                                            aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAc                                                                                  <  2:61257/150‑1 (MQ=255)
                                                             aaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAcc                                                                                 >  1:464232/1‑150 (MQ=255)
                                                                 gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                             >  1:1698788/1‑150 (MQ=255)
                                                                   tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                           >  2:265189/1‑150 (MQ=255)
                                                                      aaaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAac                                                                        <  2:134128/150‑1 (MQ=255)
                                                                            tctctGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                                  <  1:746321/150‑1 (MQ=255)
                                                                             ctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCg                                                                 >  2:1604560/1‑150 (MQ=255)
                                                                               ctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCCACACCTCCGCt                                                               >  2:750203/1‑150 (MQ=255)
                                                                                   ctaTCTGTACGATAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCACTAGCGCAGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                           <  1:1809277/148‑1 (MQ=255)
                                                                                   cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                           <  2:40298/150‑1 (MQ=255)
                                                                                   cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                           <  1:670794/150‑1 (MQ=255)
                                                                                   cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                           <  1:1612694/150‑1 (MQ=255)
                                                                                   cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                           <  2:1709127/150‑1 (MQ=255)
                                                                                         gTACGAGAAGATAACTAAATCAATGGCTTGATAAATAACCGAATGGTGGCAATAGCTCCGCCATTCTGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCg                                                     <  2:1069492/150‑1 (MQ=255)
                                                                                          tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                    <  2:863915/150‑1 (MQ=255)
                                                                                          tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCCTCCAGCTCCGc                                                    <  1:87017/150‑1 (MQ=255)
                                                                                            cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCTTTGCAACACCTCCGCTTCCCGCTCCGATa                                                  >  2:349785/1‑150 (MQ=255)
                                                                                            cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                  >  2:373194/1‑150 (MQ=255)
                                                                                               taagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                               <  1:1582713/149‑1 (MQ=255)
                                                                                               gaagaTAACTAAAGCTCTGGCTTTATAAATAACAGAATTGCGGCAATTGCGCCGCAATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCTATTCCAGCTCCGCTAATc                                               <  2:797186/150‑1 (MQ=255)
                                                                                               gaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                               <  2:1539524/150‑1 (MQ=255)
                                                                                                  taTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCa                                            <  2:244567/149‑1 (MQ=255)
                                                                                                     aaCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCATGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACAACCGCTTCCAGCTCCGCTAATCGccccc                                         >  1:1286131/1‑149 (MQ=255)
                                                                                                      aCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGa                                        >  1:378374/1‑150 (MQ=255)
                                                                                                      aCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCGGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGATTCCAGCTCCGCTAATCGCACCgc                                        >  2:1153497/1‑149 (MQ=255)
                                                                                                       cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTCCCCCTGTTTTCTCAGGCGCGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAAGGGCCCCGAc                                       >  2:1659119/1‑150 (MQ=255)
                                                                                                       cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAc                                       <  2:832664/150‑1 (MQ=255)
                                                                                                       cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAAAGAGCCGCAATTCGGCGAATTTACCCCTGTTATCTCAGCCGAGTTTCAGAATAACCACGTTGCAACACACGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcagcc                                       >  1:1352810/1‑146 (MQ=255)
                                                                                                                 ggCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                             <  1:1235858/150‑1 (MQ=255)
                                                                                                                  gCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcg                            <  2:1465233/150‑1 (MQ=255)
                                                                                                                   cTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcgc                           >  2:1766970/1‑150 (MQ=255)
                                                                                                                    ttGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcgcg                          <  1:1423456/150‑1 (MQ=255)
                                                                                                                        taaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCTCCGCCCCTAATCGACGCGGACg                      >  1:1209017/1‑150 (MQ=255)
                                                                                                                         aaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGCCGCGGACgg                     >  2:1795982/1‑150 (MQ=255)
                                                                                                                          aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                    >  2:131796/1‑150 (MQ=255)
                                                                                                                          aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                    <  1:373194/150‑1 (MQ=255)
                                                                                                                          aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                    >  2:1849830/1‑150 (MQ=255)
                                                                                                                          aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTAACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCCCCGACCCTAATCGACGCGGACggg                    >  1:1864889/1‑150 (MQ=255)
                                                                                                                            taaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggg                  <  1:377897/150‑1 (MQ=255)
                                                                                                                             aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGGTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAACGCCACTAATCGACGCGGACgggggg                 >  1:455076/1‑149 (MQ=255)
                                                                                                                              cccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCTCTAATCGCACAGACCCTAATCGACGCGGCCGGGGGat                >  2:73256/2‑150 (MQ=255)
                                                                                                                              cccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggggt                >  1:1869017/2‑148 (MQ=255)
                                                                                                                                cGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCCCCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGatat              >  2:1407293/1‑150 (MQ=255)
                                                                                                                                 gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCGGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc             <  2:921050/150‑1 (MQ=255)
                                                                                                                                  aaTGGCGTCATTATCTCCTCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCCAGTTTCAGAGTAGCCACCTTGCCTCCCCCGTTGCACCACCTCCGCTTCCAGCACCGCTATACGCAGCGACCATAATCGACGCGCACGGGGGCTAGcc            >  2:1136238/1‑150 (MQ=255)
                                                                                                                                      gcggcAATCGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGGTTCAGATTCACCACGTAGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGTGGATATCCACTg        <  1:1207425/150‑1 (MQ=255)
                                                                                                                                      gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTg        <  2:17780/150‑1 (MQ=255)
                                                                                                                                        ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGCTACTCGCACCGACCCTACTCGCCGCGGACGGGGGATATCCACGGTc      >  1:496992/1‑150 (MQ=255)
                                                                                                                                        ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGCCGGGGGATATCCACTGTc      >  1:1678602/1‑150 (MQ=255)
                                                                                                                                            aTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATGCACTGTCAAAt  >  1:1810911/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
CGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAAT  >  NC_000913/993115‑993404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: