Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,048,429 C→T 6.9% W88* (TGG→TAG) ‡ gfcC ← putative O‑antigen capsule production periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,048,4290CT 6.9% 354.1 / 10.7 116W88* (TGG→TAG) ‡gfcCputative O‑antigen capsule production periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (55/53);  new base T (3/5);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 120 total aligned reads displayed.

CCCCGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAA  >  NC_000913/1048280‑1048576
                                                                                                                                                     |                                                                                                                                                   
ccccGACCAACGGGGGATTGCTGTTCTCATCCCCTCCTACAAAATCACGGTCCAGCTTGACCGGCAAGCGCCCAGTGATAGTGTCATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCCTCAGCTTCTGCTTccc                                                                                                                                                     <  2:262466/150‑1 (MQ=255)
 cccGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATTGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCa                                                                                                                                                    >  2:567062/1‑150 (MQ=255)
          cGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcaga                                                                                                                                           <  1:1064953/150‑3 (MQ=255)
          cGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGaagcaag                                                                                                                                           <  1:1245924/150‑1 (MQ=255)
           gggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaa                                                                                                                                          <  1:534990/150‑4 (MQ=255)
           gggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGaagcaagc                                                                                                                                          >  2:1173128/1‑150 (MQ=255)
            ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                                         <  2:1631703/150‑5 (MQ=255)
            ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                                         <  2:578509/150‑5 (MQ=255)
            ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCt                                                                                                                                         <  2:1857981/150‑1 (MQ=255)
               gATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCtgtg                                                                                                                                      <  1:30332/150‑1 (MQ=255)
                aTTGCTGTTCTCATCCTCTCGTACAAAATCAGGGTCCAGTTTGACCGGTAATCGAGCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGGCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGc                                                                                                                                     <  2:1591310/150‑1 (MQ=255)
                 ttGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATCTTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGcc                                                                                                                                    <  1:274570/150‑1 (MQ=255)
                 ttGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGcc                                                                                                                                    >  1:1630732/1‑150 (MQ=255)
                  tGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCa                                                                                                                                   <  1:1797218/150‑1 (MQ=255)
                   gCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCAt                                                                                                                                  <  1:201230/150‑1 (MQ=255)
                       ttCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACa                                                                                                                              <  2:1075171/150‑1 (MQ=255)
                        tctcATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAt                                                                                                                             <  2:707144/150‑1 (MQ=255)
                            aTCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTACCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAAGTTg                                                                                                                         >  2:666038/1‑150 (MQ=255)
                            aTCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACCTGTTg                                                                                                                         >  2:966571/1‑150 (MQ=255)
                             tCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGCCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGtttg                                                                                                                        >  2:885419/1‑148 (MQ=255)
                             tCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTgg                                                                                                                        <  1:592604/150‑1 (MQ=255)
                             tCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTgg                                                                                                                        <  2:1520843/150‑1 (MQ=255)
                                ccacGTACAAAATCAGGGTCCAGTTTGACCGGAAAGCGACCAGGGATATTCAGATTGAGTAACTGCTCGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAg                                                                                                                     <  2:1247804/147‑1 (MQ=255)
                                aCTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAg                                                                                                                     <  2:577318/150‑1 (MQ=255)
                                      aCAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTa                                                                                                               <  1:1131141/150‑1 (MQ=255)
                                       cAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTAAAACGTTGCCGCCACATCGTAGTCAGCTTCTGCTTCCCAGGAAGCAAGCTCTGACATGACATGTTGGTAGTCTATaa                                                                                                              >  1:1727212/1‑150 (MQ=255)
                                        aaaaTCAGGGTCCTGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCGGCCACTTCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTatg                                                                                                             <  1:223364/150‑3 (MQ=255)
                                         aaaTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgc                                                                                                            >  2:249123/1‑150 (MQ=255)
                                           aTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgcgc                                                                                                          >  1:488337/1‑150 (MQ=255)
                                            tCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                                         >  2:1100872/1‑150 (MQ=255)
                                            tCAGCGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                                         <  2:1776923/150‑1 (MQ=255)
                                             cAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCtt                                                                                                        <  1:867376/150‑1 (MQ=255)
                                             cAGGGTCCAATTTGACCGGTAAGCGCCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACTTGTTGGTAGTGTTTAAGCGCtt                                                                                                        >  2:321429/1‑150 (MQ=255)
                                               gggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTa                                                                                                      >  2:355353/1‑150 (MQ=255)
                                                ggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:1507259/150‑1 (MQ=255)
                                                ggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:1759264/150‑1 (MQ=255)
                                                gctCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGACTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:664108/148‑1 (MQ=255)
                                                 gTCCAGGTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGc                                                                                                    <  2:892826/150‑1 (MQ=255)
                                                   ccAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCtt                                                                                                  >  1:1085415/1‑150 (MQ=255)
                                                     aGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTc                                                                                                >  1:1260248/1‑150 (MQ=255)
                                                     aGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTc                                                                                                <  2:10068/150‑1 (MQ=255)
                                                      gTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcg                                                                                               >  2:563744/1‑150 (MQ=255)
                                                       tttGACCGGTACGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgc                                                                                              <  2:1765862/150‑1 (MQ=255)
                                                          gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           <  2:1375097/150‑1 (MQ=255)
                                                          gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           >  2:1092655/1‑150 (MQ=255)
                                                          gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           >  2:1025808/1‑150 (MQ=255)
                                                          gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           >  1:411161/1‑150 (MQ=255)
                                                                 atgCGACCAGTGATATTTAGATTGAGTAACTGCTGGCGCACGGAGTTAATCGGTGCCGCCACATCGTGGTGAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCg                                                                                    <  1:1622130/148‑1 (MQ=255)
                                                                 aaGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCg                                                                                    <  1:543814/150‑1 (MQ=255)
                                                                  aGCGACCAGTGATATTGAGATTGAGTAAATGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTATGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGGAGATTCgg                                                                                   >  2:828617/1‑150 (MQ=255)
                                                                     gACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCAACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAATAGCGTTAGCTTTCGCCGCAGAGTCGGTCa                                                                                >  2:584677/1‑150 (MQ=255)
                                                                      aCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAg                                                                               >  1:873362/1‑150 (MQ=255)
                                                                      aCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAg                                                                               >  2:864447/1‑150 (MQ=255)
                                                                      aCCAGTGATATTGAGATTGAGTAACTGCTCGCGCACCGATTTAATCGTTCCCGCCACATCGTCGTCAGCTTCTGCTGCCCAGGAAGCAAGCAGTGCCCTGACAAGTTGGTAGTCTTTAAGCTCTTTAGCTTTCGCCGCAGAGTCGGTccc                                                                               >  2:802779/1‑148 (MQ=255)
                                                                           tGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCGTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAgg                                                                          <  1:1069126/150‑1 (MQ=255)
                                                                            gATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGc                                                                         <  2:414726/150‑1 (MQ=255)
                                                                            gATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGc                                                                         <  2:1403308/150‑1 (MQ=255)
                                                                            gATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGc                                                                         <  1:1187111/150‑1 (MQ=255)
                                                                               attgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCAcc                                                                      >  2:277088/1‑150 (MQ=255)
                                                                                ttgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCa                                                                     >  1:65045/1‑150 (MQ=255)
                                                                                ttgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCa                                                                     >  1:1018156/1‑150 (MQ=255)
                                                                                ttcagattgagTAACTGCTGGCGCACGGAGTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTACCCAGGAAGCAAGCTGTGCCATGACATGATGGTAGTCAGTAAGCGCTTTAGCTTTAGCCGCATAGTCGGTCAGCAAGGCACCa                                                                     >  2:839/4‑150 (MQ=255)
                                                                                 tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCTCCAg                                                                    >  1:1119812/1‑150 (MQ=255)
                                                                                 tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAg                                                                    >  1:1294292/1‑150 (MQ=255)
                                                                                   agattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGc                                                                  >  2:455396/1‑150 (MQ=255)
                                                                                          gTAACTGCTGGCGCACGGATTAAATCGTTGCCGCCACATCGTCGGCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTAAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCaga                                                           <  2:1118176/150‑1 (MQ=255)
                                                                                            aaCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAgg                                                         <  2:1575277/150‑1 (MQ=255)
                                                                                             aCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGc                                                        <  1:1734448/150‑1 (MQ=255)
                                                                                             aCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGAACCAGGCCACCAGAggg                                                        >  1:1548345/1‑149 (MQ=255)
                                                                                                gctgGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGAt                                                     >  1:1400998/1‑150 (MQ=255)
                                                                                                  tgGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATcg                                                   <  1:216088/150‑1 (MQ=255)
                                                                                                   ggCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATcgc                                                  >  2:1639585/1‑150 (MQ=255)
                                                                                                        aCGGATTTAATCGATGCCGCCACATCATCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGGAGCTAGTCTTTACGCGCAGTAGCTGTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCGCCAAGAGGCGCTCGCGAAGt                                             >  1:81520/1‑150 (MQ=255)
                                                                                                           gATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCCCCAGGCCACCAGAGGCGATCGCGCAGTTGc                                          >  2:1501592/1‑150 (MQ=255)
                                                                                                             tttAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCgg                                        >  2:255656/1‑150 (MQ=255)
                                                                                                             tttAATCGTTGCCGCCACATCGTCGTCAGCTTATGCTTACCAGGAAGCAAGCTGTGCCATGACATGTTGGTTGTGTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCCCCAGGCCACAAGAGGCGATCGCGGAGTTGCgg                                        >  1:1688445/1‑150 (MQ=255)
                                                                                                              ttAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGt                                       <  2:240241/150‑1 (MQ=255)
                                                                                                               tAATCGTTGCCGACACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCAATGACATGATGGTAGTCTTTAAGCGCATTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGtt                                      >  2:831380/1‑150 (MQ=255)
                                                                                                                actCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCGTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     >  2:923171/3‑150 (MQ=255)
                                                                                                                aaTCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  1:1410240/150‑1 (MQ=255)
                                                                                                                aaTCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTAAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  2:46101/150‑1 (MQ=255)
                                                                                                                aaTCGTTGCCGCCACATCGTCGGCAGCTTCTGCTTCCCTGGAAGCAAGCTGTGCCATGACATGGTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  1:1452843/150‑1 (MQ=255)
                                                                                                                  tCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCCGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   >  1:1653506/1‑150 (MQ=255)
                                                                                                                  tCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   <  2:153643/150‑1 (MQ=255)
                                                                                                                  tCGTTGCCGCCACATCGTCGGCAGCTTCTGCTTCCCTGGAAGCAAGCTGTGCCATGTCATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   <  2:732478/150‑1 (MQ=255)
                                                                                                                   cGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTgtgt                                  <  1:561465/150‑1 (MQ=255)
                                                                                                                    ggtGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTc                                 <  1:321429/148‑1 (MQ=255)
                                                                                                                       gccgccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCAcc                              <  1:1403125/150‑1 (MQ=255)
                                                                                                                       gccgccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTATTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGACAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCAcc                              >  2:654320/1‑150 (MQ=255)
                                                                                                                          gccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAGGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAAc                           >  1:1182718/1‑150 (MQ=255)
                                                                                                                             acaTCGTCGTCAGCTTCTGCGTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTgg                        <  2:1159075/150‑1 (MQ=255)
                                                                                                                              caTCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGa                       <  2:1085415/150‑1 (MQ=255)
                                                                                                                                tcgtcgTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGaca                     <  2:1766123/150‑1 (MQ=255)
                                                                                                                                  gtcgtcAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGacatc                   >  2:950480/1‑148 (MQ=255)
                                                                                                                                        aGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCCGCAAGGCACCAGGCCACCAGCGGCGCTCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTc             >  1:1504967/1‑150 (MQ=255)
                                                                                                                                           gcttGCTTCCTGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCCGGCCACCAGAGGCGATCGCGCAGGTGCGGTTGTGTCACCAACTGGACAACATTTTCCAc          >  2:1653122/4‑150 (MQ=255)
                                                                                                                                           gcttGCTTCCTGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCAc          >  1:1537372/4‑150 (MQ=255)
                                                                                                                                             cTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACgg        <  1:203339/150‑1 (MQ=255)
                                                                                                                                               gCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACCGGACAACATTTTCCACgggg      >  1:1332072/1‑150 (MQ=255)
                                                                                                                                                   cccAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAAGATTTTCCACGGGGCCaa  >  2:1287763/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCCCGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAA  >  NC_000913/1048280‑1048576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: