Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,048,430 A→G 7.0% W88R (TGG→CGG) ‡ gfcC ← putative O‑antigen capsule production periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,048,4300AG 7.0% 337.0 / 11.9 117W88R (TGG→CGG) ‡gfcCputative O‑antigen capsule production periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (54/52);  new base G (3/5);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 118 total aligned reads displayed.

CCCGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAA  >  NC_000913/1048281‑1048576
                                                                                                                                                     |                                                                                                                                                  
cccGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATTGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCa                                                                                                                                                    >  2:567062/1‑150 (MQ=255)
         cGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcaga                                                                                                                                           <  1:1064953/150‑3 (MQ=255)
         cGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGaagcaag                                                                                                                                           <  1:1245924/150‑1 (MQ=255)
          gggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaa                                                                                                                                          <  1:534990/150‑4 (MQ=255)
          gggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGaagcaagc                                                                                                                                          >  2:1173128/1‑150 (MQ=255)
           ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                                         <  2:578509/150‑5 (MQ=255)
           ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                                         <  2:1631703/150‑5 (MQ=255)
           ggggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCt                                                                                                                                         <  2:1857981/150‑1 (MQ=255)
            gggATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCATCGTCAGCTTCTGCTTCCTGGGaagcagaagc                                                                                                                                        <  1:27354/150‑6 (MQ=255)
              gATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCtgtg                                                                                                                                      <  1:30332/150‑1 (MQ=255)
                ttGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATCTTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGcc                                                                                                                                    <  1:274570/150‑1 (MQ=255)
                ttGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGcc                                                                                                                                    >  1:1630732/1‑150 (MQ=255)
                 tGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCa                                                                                                                                   <  1:1797218/150‑1 (MQ=255)
                  gCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCAt                                                                                                                                  <  1:201230/150‑1 (MQ=255)
                      ttCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACa                                                                                                                              <  2:1075171/150‑1 (MQ=255)
                       tctcATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAt                                                                                                                             <  2:707144/150‑1 (MQ=255)
                           aTCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTACCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAAGTTg                                                                                                                         >  2:666038/1‑150 (MQ=255)
                           aTCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACCTGTTg                                                                                                                         >  2:966571/1‑150 (MQ=255)
                            tCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGCCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGtttg                                                                                                                        >  2:885419/1‑148 (MQ=255)
                            tCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTgg                                                                                                                        <  2:1520843/150‑1 (MQ=255)
                               ccacGTACAAAATCAGGGTCCAGTTTGACCGGAAAGCGACCAGGGATATTCAGATTGAGTAACTGCTCGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAg                                                                                                                     <  2:1247804/147‑1 (MQ=255)
                               aCTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAg                                                                                                                     <  2:577318/150‑1 (MQ=255)
                                 tCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTc                                                                                                                   >  2:619543/1‑150 (MQ=255)
                                   gTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCtt                                                                                                                 <  1:1737660/150‑1 (MQ=255)
                                      cAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTAAAACGTTGCCGCCACATCGTAGTCAGCTTCTGCTTCCCAGGAAGCAAGCTCTGACATGACATGTTGGTAGTCTATaa                                                                                                              >  1:1727212/1‑150 (MQ=255)
                                       aaaaTCAGGGTCCTGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCGGCCACTTCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTatg                                                                                                             <  1:223364/150‑3 (MQ=255)
                                        aaaTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgc                                                                                                            >  2:249123/1‑150 (MQ=255)
                                          aTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgcgc                                                                                                          >  1:488337/1‑150 (MQ=255)
                                           tCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                                         >  1:710174/1‑150 (MQ=255)
                                           tCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                                         >  2:1100872/1‑150 (MQ=255)
                                           tCAGCGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                                         <  2:1776923/150‑1 (MQ=255)
                                            cAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCtt                                                                                                        <  1:867376/150‑1 (MQ=255)
                                            cAGGGTCCAATTTGACCGGTAAGCGCCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACTTGTTGGTAGTGTTTAAGCGCtt                                                                                                        >  2:321429/1‑150 (MQ=255)
                                              gggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTa                                                                                                      >  2:355353/1‑150 (MQ=255)
                                               ggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:1507259/150‑1 (MQ=255)
                                               ggTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:1759264/150‑1 (MQ=255)
                                               gctCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGACTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAg                                                                                                     <  2:664108/148‑1 (MQ=255)
                                                gTCCAGGTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGc                                                                                                    <  2:892826/150‑1 (MQ=255)
                                                  ccAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCtt                                                                                                  >  1:1085415/1‑150 (MQ=255)
                                                    aGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTc                                                                                                >  1:1260248/1‑150 (MQ=255)
                                                    aGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTc                                                                                                <  2:10068/150‑1 (MQ=255)
                                                     gTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcg                                                                                               >  2:563744/1‑150 (MQ=255)
                                                         gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           >  2:1092655/1‑150 (MQ=255)
                                                         gACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTcgccgc                                                                                           <  2:1375097/150‑1 (MQ=255)
                                                             ggTAAGCGACCAGTGATAATGAGATTGAGTAACTGCTGGCGAAAGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTGCCCCGGAAGAAAGCGTTGCCATGACATGTTGGTAGGATTTAAGCGCTTTATCTTTCGCCGCagac                                                                                       >  1:126954/1‑149 (MQ=255)
                                                                atgCGACCAGTGATATTTAGATTGAGTAACTGCTGGCGCACGGAGTTAATCGGTGCCGCCACATCGTGGTGAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCg                                                                                    <  1:1622130/148‑1 (MQ=255)
                                                                aaGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCg                                                                                    <  1:543814/150‑1 (MQ=255)
                                                                 aGCGACCAGTGATATTGAGATTGAGTAAATGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTATGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGGAGATTCgg                                                                                   >  2:828617/1‑150 (MQ=255)
                                                                     aCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAg                                                                               >  1:873362/1‑150 (MQ=255)
                                                                     aCCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAg                                                                               >  2:864447/1‑150 (MQ=255)
                                                                     aCCAGTGATATTGAGATTGAGTAACTGCTCGCGCACCGATTTAATCGTTCCCGCCACATCGTCGTCAGCTTCTGCTGCCCAGGAAGCAAGCAGTGCCCTGACAAGTTGGTAGTCTTTAAGCTCTTTAGCTTTCGCCGCAGAGTCGGTccc                                                                               >  2:802779/1‑148 (MQ=255)
                                                                         gTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAg                                                                           <  2:1539557/150‑1 (MQ=255)
                                                                          tGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCGTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAgg                                                                          <  1:1069126/150‑1 (MQ=255)
                                                                           gATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGc                                                                         <  2:414726/150‑1 (MQ=255)
                                                                           gATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGc                                                                         <  1:1187111/150‑1 (MQ=255)
                                                                              attgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCAcc                                                                      >  2:277088/1‑150 (MQ=255)
                                                                               ttgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCa                                                                     >  1:1018156/1‑150 (MQ=255)
                                                                               ttgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCa                                                                     >  1:65045/1‑150 (MQ=255)
                                                                               ttcagattgagTAACTGCTGGCGCACGGAGTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTACCCAGGAAGCAAGCTGTGCCATGACATGATGGTAGTCAGTAAGCGCTTTAGCTTTAGCCGCATAGTCGGTCAGCAAGGCACCa                                                                     >  2:839/4‑150 (MQ=255)
                                                                                tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCTCCAg                                                                    >  1:1119812/1‑150 (MQ=255)
                                                                                tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAg                                                                    >  2:1403125/1‑150 (MQ=255)
                                                                                tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAg                                                                    >  1:1294292/1‑150 (MQ=255)
                                                                                tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTAGCCGCAGAGTCGGTCAGCAAGGCACCAg                                                                    >  1:1449728/1‑150 (MQ=255)
                                                                                  agattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGc                                                                  >  2:299298/1‑150 (MQ=255)
                                                                                         gTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCaga                                                           <  2:171131/150‑1 (MQ=255)
                                                                                         gTAACTGCTGGCGCACGGATTAAATCGTTGCCGCCACATCGTCGGCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTAAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCaga                                                           <  2:1118176/150‑1 (MQ=255)
                                                                                           acctgcggGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTGCTGCTTCCCAGGACGCAAGCTGTGCCATGACATGGGGGTAGTCTTTCAGCGCTTTAGCTGTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAgg                                                         <  1:1120367/148‑1 (MQ=255)
                                                                                           aaCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAgg                                                         <  2:1575277/150‑1 (MQ=255)
                                                                                            aCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGc                                                        <  1:1734448/150‑1 (MQ=255)
                                                                                            aCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGAACCAGGCCACCAGAggg                                                        >  1:1548345/1‑149 (MQ=255)
                                                                                               gctgGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGAt                                                     >  1:1400998/1‑150 (MQ=255)
                                                                                                 tgGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATcg                                                   <  1:216088/150‑1 (MQ=255)
                                                                                                  ggCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATcgc                                                  >  2:1639585/1‑150 (MQ=255)
                                                                                                       aCGGATTTAATCGATGCCGCCACATCATCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGGAGCTAGTCTTTACGCGCAGTAGCTGTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCGCCAAGAGGCGCTCGCGAAGt                                             >  1:81520/1‑150 (MQ=255)
                                                                                                          gATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCCCCAGGCCACCAGAGGCGATCGCGCAGTTGc                                          >  2:1501592/1‑150 (MQ=255)
                                                                                                            tttAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCgg                                        >  1:496465/1‑150 (MQ=255)
                                                                                                             ttAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGt                                       <  2:240241/150‑1 (MQ=255)
                                                                                                              tAATCGTTGCCGACACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCAATGACATGATGGTAGTCTTTAAGCGCATTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGtt                                      >  2:831380/1‑150 (MQ=255)
                                                                                                               actCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCGTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     >  2:923171/3‑150 (MQ=255)
                                                                                                               aaTCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  1:1410240/150‑1 (MQ=255)
                                                                                                               aaTCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTAAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  2:46101/150‑1 (MQ=255)
                                                                                                               aaTCGTTGCCGCCACATCGTCGGCAGCTTCTGCTTCCCTGGAAGCAAGCTGTGCCATGACATGGTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtg                                     <  1:1452843/150‑1 (MQ=255)
                                                                                                                 tCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCCGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   >  1:1653506/1‑150 (MQ=255)
                                                                                                                 tCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   <  2:223842/150‑1 (MQ=255)
                                                                                                                 tCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTtgtg                                   <  2:153643/150‑1 (MQ=255)
                                                                                                                  cGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTgtgt                                  <  1:561465/150‑1 (MQ=255)
                                                                                                                   ggtGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTc                                 <  1:321429/148‑1 (MQ=255)
                                                                                                                   gTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTc                                 >  2:741437/1‑150 (MQ=255)
                                                                                                                      gccgccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCAcc                              <  1:1403125/150‑1 (MQ=255)
                                                                                                                      gccgccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTATTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGACAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCAcc                              >  2:654320/1‑150 (MQ=255)
                                                                                                                         gccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACAGGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAAc                           >  1:1182718/1‑150 (MQ=255)
                                                                                                                            acaTCGTCGTCAGCTTCTGCGTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTgg                        <  2:1159075/150‑1 (MQ=255)
                                                                                                                             caTCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGa                       <  2:1085415/150‑1 (MQ=255)
                                                                                                                               tcgtcgTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGaca                     <  2:1766123/150‑1 (MQ=255)
                                                                                                                                 gtcgtcAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGacatc                   >  2:950480/1‑148 (MQ=255)
                                                                                                                                          gcttGCTTCCTGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCCGGCCACCAGAGGCGATCGCGCAGGTGCGGTTGTGTCACCAACTGGACAACATTTTCCAc          >  2:1653122/4‑150 (MQ=255)
                                                                                                                                          gcttGCTTCCTGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCAc          >  1:1537372/4‑150 (MQ=255)
                                                                                                                                              gCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACgggg      >  1:1629127/1‑150 (MQ=255)
                                                                                                                                                  cccAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAAGATTTTCCACGGGGCCaa  >  2:1287763/1‑150 (MQ=255)
                                                                                                                                                  cccAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCaa  <  2:488337/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCCGACCAACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAA  >  NC_000913/1048281‑1048576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: