Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,932 C→T 5.5% intergenic (+30/‑29) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9320CT 5.5% 399.9 / 5.4 126intergenic (+30/‑29)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base C (60/59);  new base T (2/5);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCA  >  NC_000913/1104783‑1105078
                                                                                                                                                     |                                                                                                                                                  
aTGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGgcgc                                                                                                                                                    <  1:38186/150‑1 (MQ=255)
 tGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCa                                                                                                                                                   >  2:571929/1‑150 (MQ=255)
    cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCTGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                >  1:376593/1‑150 (MQ=255)
     ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                               <  2:592272/150‑1 (MQ=255)
     ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATATAACTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                               <  2:779777/150‑1 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                          >  2:990830/1‑150 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                          <  1:1166428/150‑1 (MQ=255)
           aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                         <  2:1078610/150‑1 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCTGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttc                                                                                                                                        <  1:1245893/150‑2 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTTAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttt                                                                                                                                       >  1:1105754/1‑150 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttct                                                                                                                                       <  1:84477/150‑3 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttt                                                                                                                                       <  2:1773372/150‑1 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCACATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttct                                                                                                                                       <  2:326716/150‑3 (MQ=255)
               ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                     <  2:215888/150‑1 (MQ=255)
                tCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttctgta                                                                                                                                    <  1:1518866/150‑6 (MQ=255)
                 cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                   >  1:1604603/1‑150 (MQ=255)
                  ggtggtGCCAACTGTGCTGCAGTTGACCAGACTGAATCTAACTCCTCGGTCAAGGTGACTCAGGTTGGCTTTGTTAACAACGCGACCGCTCATCTGTTCAAATACATCATTTGTATTACAGAAACAGGGCGCTTGCCCTGTTTTTTTTCg                                                                                                                                  <  2:1096202/150‑1 (MQ=255)
                   gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                 >  2:1039114/1‑150 (MQ=255)
                    tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCCTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATTCATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                <  2:266547/150‑1 (MQ=255)
                       tggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCGCTGGTTTTTTTCGGgaga                                                                                                                             >  2:1817512/1‑150 (MQ=255)
                            aCGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGGTTTTTTTCGGGAGAAGAat                                                                                                                        >  1:1643998/1‑150 (MQ=255)
                             cGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAata                                                                                                                       >  1:946847/1‑150 (MQ=255)
                             cGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAata                                                                                                                       <  1:1780933/150‑1 (MQ=255)
                                  ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                  >  1:603827/1‑150 (MQ=255)
                                     cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACg                                                                                                               >  2:1731031/1‑150 (MQ=255)
                                       gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTTAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                             <  2:1105754/150‑1 (MQ=255)
                                         tgctcataCTTCATCTAACTCCTCCGTCAACGTGACTCAGGTTTGCTTTTGTAAAAACGCGACCTCTCATCAGTACTAATAAATCATTTGTATTACAGAAACATGGCGCAATCCCTGTTTTTTTTCTGGAGAAGAATATTAATACTttat                                                                                                           <  1:456963/143‑1 (MQ=255)
                                            ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                >  1:1018588/1‑126 (MQ=255)
                                            ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                <  2:1018588/126‑1 (MQ=255)
                                            ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttattaa                                                                                                        >  1:587115/1‑149 (MQ=255)
                                              aGACTGAATCTAACTCCTCCGTCAACGTGACTCATGTTGGCTTTGGTAACAACGCGACTGCTCATCAGTACTAATACATCATTTGTATTACAGTAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTc                                                                                                      <  1:791566/150‑1 (MQ=255)
                                                aCTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCt                                                                                                    >  1:347945/1‑150 (MQ=255)
                                                    cATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCACGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCg                                                                                                >  2:359949/1‑150 (MQ=255)
                                                     aTCAAACTCCTCCGTCAACTTCACTCAGTTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                               <  1:851031/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                              <  2:606166/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                              >  1:1696773/1‑150 (MQ=255)
                                                         aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACt                                                                                           >  2:206202/1‑150 (MQ=255)
                                                          aCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCATTGCGGCACtt                                                                                          >  1:1345086/1‑150 (MQ=255)
                                                           ctcctcCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACttt                                                                                         >  1:1838990/1‑150 (MQ=255)
                                                             cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATATGTTATTACTCCTTGCGGCACTTTcc                                                                                       >  2:1473802/1‑150 (MQ=255)
                                                             cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTcc                                                                                       >  1:1422162/1‑150 (MQ=255)
                                                             cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTAGCGGCACTTTcc                                                                                       >  1:1763022/1‑150 (MQ=255)
                                                               tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  2:425437/150‑1 (MQ=255)
                                                               tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  2:1635311/150‑1 (MQ=255)
                                                                    cAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGa                                                                                <  1:1036347/150‑1 (MQ=255)
                                                                         tGACTCAGGTTGGCTTTTGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAAcc                                                                           <  1:335122/150‑1 (MQ=255)
                                                                            cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                        <  2:1356479/150‑1 (MQ=255)
                                                                                ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                    >  1:576054/1‑150 (MQ=255)
                                                                                    ggCTTTGGTAACAACGCGACCGCTCATCAGTTCTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAc                                                                <  2:545772/150‑1 (MQ=255)
                                                                                    ggCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACTTCATTTGTATTACATAAACAGGGCGCAAGCCCTGTTTTTTTTCTGGAGATGAATATGAATACGATATAACTCCTTGCGCCACTTTCCAGTCAGATAACATTAAATACGAc                                                                >  1:560593/1‑150 (MQ=255)
                                                                                          tgTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                          <  2:1274503/149‑1 (MQ=255)
                                                                                          ggTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                          >  1:511933/1‑150 (MQ=255)
                                                                                           gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCATGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCAAGTCAGATAACCTTTAATACGACCCAGCaa                                                         >  1:1121734/1‑150 (MQ=255)
                                                                                            tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                        >  2:1655503/1‑150 (MQ=255)
                                                                                               caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg                                                     <  2:1608523/150‑1 (MQ=255)
                                                                                               caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg                                                     >  1:1470639/1‑150 (MQ=255)
                                                                                               caacGCGACCGCTCATCAGTACTAATACATCATTTGAATTACACAAACAGCGCGCAAGCCCTGTTTTTTTTCGGGATAAGAATATGAATACGTTATTACTCCTAGCGGCACTTTACAGTCACATAACCTTTAATACCACCCAGCaatgtg                                                     >  1:72157/1‑146 (MQ=255)
                                                                                                aacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa                                                    <  1:612108/150‑1 (MQ=255)
                                                                                                  cgcgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGTGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                  >  2:959568/1‑150 (MQ=255)
                                                                                                    cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtgtg                                                <  2:102543/150‑1 (MQ=255)
                                                                                                       ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                             >  1:665434/1‑150 (MQ=255)
                                                                                                       ccGCTCATAAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTAAGATAACATTTATTACTAACCAGCAAGGGGATGTGtat                                             >  1:1277589/1‑150 (MQ=255)
                                                                                                         gCTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAc                                           <  2:100929/150‑1 (MQ=255)
                                                                                                          gtcatcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  2:467765/149‑1 (MQ=255)
                                                                                                          cTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGTCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  2:1157729/150‑1 (MQ=255)
                                                                                                          cTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  1:1176924/150‑1 (MQ=255)
                                                                                                          cTCATCAGTAATAATACATCATTTGTATTACAGAAACAGTGCTCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGTCACTTTCCAGTCACATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  2:245739/150‑1 (MQ=255)
                                                                                                            catcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGTAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCat                                        <  1:1424809/150‑1 (MQ=255)
                                                                                                             atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                        <  1:731561/133‑1 (MQ=255)
                                                                                                             atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatt                                       >  1:485331/1‑150 (MQ=255)
                                                                                                              tcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatta                                      >  2:1171608/1‑150 (MQ=255)
                                                                                                                aGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCGTTAATACGACCCAGCAAGGGGATGTGTATACCattatt                                    >  2:217549/1‑150 (MQ=255)
                                                                                                                  tACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTTCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTcc                                  <  1:1445298/150‑1 (MQ=255)
                                                                                                                    cTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTg                                <  1:632031/150‑1 (MQ=255)
                                                                                                                     tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                               <  2:946847/150‑1 (MQ=255)
                                                                                                                      aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                              <  1:1731031/150‑1 (MQ=255)
                                                                                                                      aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTACTTGCGGCACTTTACATTAAGATAACCGTTAATAAGACCCAGCAAGGGGATGTGTAAAACATTATTACTGaa                              >  2:777684/1‑150 (MQ=255)
                                                                                                                           atcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAc                         >  1:1358018/1‑150 (MQ=255)
                                                                                                                           atcatTTGTAGTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATAGGAATACGGTATTACTCCGGGCGGCCCTTTCCAGGCCGATTACCTTTAATACGACCCAGCAAGGGGCTGTGTATCCCATTATTCCTGAAGGCAc                         >  1:103126/1‑150 (MQ=255)
                                                                                                                             catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActc                       >  1:698649/1‑150 (MQ=255)
                                                                                                                             catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAAAACGACCCAGAAAGGGGATGTCTATACCATTATTCCTGAAGTCaata                       >  1:561586/1‑147 (MQ=255)
                                                                                                                                 tGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTAc                   >  2:410059/1‑150 (MQ=255)
                                                                                                                                    cgaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                >  1:1237776/8‑150 (MQ=255)
                                                                                                                                    aTTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                >  2:1675469/1‑150 (MQ=255)
                                                                                                                                      tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCGAGTCAGATAACATTTAATACGACCCAGGAAGGGGATGTGTATACGATTATTCCTGAAGTCAATCTTACTCAAt              >  2:156058/1‑150 (MQ=255)
                                                                                                                                      tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAt              >  2:1257317/1‑150 (MQ=255)
                                                                                                                                      tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAt              >  1:1557340/1‑150 (MQ=255)
                                                                                                                                          gAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTg          <  2:1397508/150‑1 (MQ=255)
                                                                                                                                           aaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGt         >  2:789059/1‑150 (MQ=255)
                                                                                                                                            aaCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc        <  2:249445/150‑1 (MQ=255)
                                                                                                                                             aCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt       <  1:1039114/150‑1 (MQ=255)
                                                                                                                                              cAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtg      >  2:1459486/1‑150 (MQ=255)
                                                                                                                                               aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTTTTCCTGAAGTCTATCTTACTCAATCTTGTCtat     >  1:1099394/1‑148 (MQ=255)
                                                                                                                                               aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt     <  2:25189/150‑1 (MQ=255)
                                                                                                                                                gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGTGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg    >  1:96013/1‑150 (MQ=255)
                                                                                                                                                gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg    <  2:1219135/150‑1 (MQ=255)
                                                                                                                                                gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg    >  1:1491478/1‑150 (MQ=255)
                                                                                                                                                 ggCGCATGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc   <  2:1706685/150‑1 (MQ=255)
                                                                                                                                                 ggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc   >  2:496330/1‑150 (MQ=255)
                                                                                                                                                  gcgcAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCa  <  1:990830/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCA  >  NC_000913/1104783‑1105078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: