Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,934 A→C 5.2% intergenic (+32/‑27) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9340AC 5.2% 372.3 / 8.3 127intergenic (+32/‑27)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base A (58/60);  new base C (3/4);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

Only 100 of 127 total aligned reads displayed.

CGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCA  >  NC_000913/1104787‑1105078
                                                                                                                                                   |                                                                                                                                                
cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCTGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                >  1:376593/1‑150 (MQ=255)
 ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                               <  2:592272/150‑1 (MQ=255)
 ggTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATATAACTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGcc                                                                                                                                               <  2:779777/150‑1 (MQ=255)
      aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                          >  2:990830/1‑150 (MQ=255)
       aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                         <  2:1078610/150‑1 (MQ=255)
        cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCTGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttc                                                                                                                                        <  1:1245893/150‑2 (MQ=255)
         aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTTAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttt                                                                                                                                       >  1:1105754/1‑150 (MQ=255)
         aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttct                                                                                                                                       <  1:84477/150‑3 (MQ=255)
         aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttt                                                                                                                                       <  2:1773372/150‑1 (MQ=255)
         aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCACATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttct                                                                                                                                       <  2:326716/150‑3 (MQ=255)
           ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                     <  2:215888/150‑1 (MQ=255)
            tCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttctgta                                                                                                                                    <  1:1518866/150‑6 (MQ=255)
             cGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                   >  1:1604603/1‑150 (MQ=255)
              ggtggtGCCAACTGTGCTGCAGTTGACCAGACTGAATCTAACTCCTCGGTCAAGGTGACTCAGGTTGGCTTTGTTAACAACGCGACCGCTCATCTGTTCAAATACATCATTTGTATTACAGAAACAGGGCGCTTGCCCTGTTTTTTTTCg                                                                                                                                  <  2:1096202/150‑1 (MQ=255)
               gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                 >  2:1039114/1‑150 (MQ=255)
                tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCCTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATTCATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                <  2:266547/150‑1 (MQ=255)
                 ggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGga                                                                                                                               >  1:434588/1‑150 (MQ=255)
                   tggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCGCTGGTTTTTTTCGGgaga                                                                                                                             >  2:1817512/1‑150 (MQ=255)
                         cGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAata                                                                                                                       >  1:946847/1‑150 (MQ=255)
                         cGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAata                                                                                                                       <  1:1780933/150‑1 (MQ=255)
                              ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                  >  1:603827/1‑150 (MQ=255)
                                 cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACg                                                                                                               <  1:656554/150‑1 (MQ=255)
                                   gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTTAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                             <  2:1105754/150‑1 (MQ=255)
                                     tgctcataCTTCATCTAACTCCTCCGTCAACGTGACTCAGGTTTGCTTTTGTAAAAACGCGACCTCTCATCAGTACTAATAAATCATTTGTATTACAGAAACATGGCGCAATCCCTGTTTTTTTTCTGGAGAAGAATATTAATACTttat                                                                                                           <  1:456963/143‑1 (MQ=255)
                                        ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                >  1:1018588/1‑126 (MQ=255)
                                        ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                <  2:1018588/126‑1 (MQ=255)
                                        ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttattaa                                                                                                        >  1:587115/1‑149 (MQ=255)
                                          aGACTGAATCTAACTCCTCCGTCAACGTGACTCATGTTGGCTTTGGTAACAACGCGACTGCTCATCAGTACTAATACATCATTTGTATTACAGTAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTc                                                                                                      <  1:791566/150‑1 (MQ=255)
                                              tGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTg                                                                                                  <  2:536615/150‑1 (MQ=255)
                                               gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                 >  1:25189/1‑150 (MQ=255)
                                                 aTCAAACTCCTCCGTCAACTTCACTCAGTTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                               <  1:851031/150‑1 (MQ=255)
                                                  tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTTTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                              <  1:1261784/150‑1 (MQ=255)
                                                  tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                              <  2:226733/150‑1 (MQ=255)
                                                  tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                              >  1:1696773/1‑150 (MQ=255)
                                                      aCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCATTGCGGCACtt                                                                                          >  1:1345086/1‑150 (MQ=255)
                                                       ctcctcCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACttt                                                                                         >  1:1838990/1‑150 (MQ=255)
                                                         cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATATGTTATTACTCCTTGCGGCACTTTcc                                                                                       >  2:1473802/1‑150 (MQ=255)
                                                           tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACTCGACCGCTCAACAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  1:1128752/150‑1 (MQ=255)
                                                           tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  2:1148999/150‑1 (MQ=255)
                                                           tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  2:425437/150‑1 (MQ=255)
                                                           tccGTCAACGTGAATCAGGTTGGCTTTTGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTAGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                     <  1:140626/150‑1 (MQ=255)
                                                            ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGt                                                                                    <  2:1454540/150‑1 (MQ=255)
                                                              gTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCa                                                                                  <  1:1483543/150‑1 (MQ=255)
                                                                cAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGa                                                                                <  1:1036347/150‑1 (MQ=255)
                                                                     tGACTCAGGTTGGCTTTTGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAAcc                                                                           <  1:335122/150‑1 (MQ=255)
                                                                     tGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAAcc                                                                           <  2:1561581/150‑1 (MQ=255)
                                                                     tGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAAcc                                                                           <  2:1785563/150‑1 (MQ=255)
                                                                        cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                        <  2:1356479/150‑1 (MQ=255)
                                                                            ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                    >  1:576054/1‑150 (MQ=255)
                                                                                ggCTTTGGTAACAACGCGACCGCTCATCAGTTCTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAc                                                                <  2:545772/150‑1 (MQ=255)
                                                                                ggCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACTTCATTTGTATTACATAAACAGGGCGCAAGCCCTGTTTTTTTTCTGGAGATGAATATGAATACGATATAACTCCTTGCGCCACTTTCCAGTCAGATAACATTAAATACGAc                                                                >  1:560593/1‑150 (MQ=255)
                                                                                      tgTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                          <  2:1274503/149‑1 (MQ=255)
                                                                                       gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCATGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCAAGTCAGATAACCTTTAATACGACCCAGCaa                                                         >  1:1121734/1‑150 (MQ=255)
                                                                                        tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                        >  2:1655503/1‑150 (MQ=255)
                                                                                           caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg                                                     <  2:1608523/150‑1 (MQ=255)
                                                                                           caacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg                                                     >  1:1470639/1‑150 (MQ=255)
                                                                                           caacGCGACCGCTCATCAGTACTAATACATCATTTGAATTACACAAACAGCGCGCAAGCCCTGTTTTTTTTCGGGATAAGAATATGAATACGTTATTACTCCTAGCGGCACTTTACAGTCACATAACCTTTAATACCACCCAGCaatgtg                                                     >  1:72157/1‑146 (MQ=255)
                                                                                            aacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa                                                    <  1:612108/150‑1 (MQ=255)
                                                                                              cgcgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGTGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                  >  2:959568/1‑150 (MQ=255)
                                                                                                cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtgtg                                                <  2:102543/150‑1 (MQ=255)
                                                                                                   ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                             >  1:665434/1‑150 (MQ=255)
                                                                                                     gCTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAc                                           <  2:100929/150‑1 (MQ=255)
                                                                                                      cTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  1:1176924/150‑1 (MQ=255)
                                                                                                      cTCATCAGTAATAATACATCATTTGTATTACAGAAACAGTGCTCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGTCACTTTCCAGTCACATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                          <  2:245739/150‑1 (MQ=255)
                                                                                                        catcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGTAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCat                                        <  1:1424809/150‑1 (MQ=255)
                                                                                                         atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                        >  2:731561/1‑133 (MQ=255)
                                                                                                         atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                        <  1:731561/133‑1 (MQ=255)
                                                                                                         atcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatt                                       >  1:485331/1‑150 (MQ=255)
                                                                                                          tcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatta                                      >  2:1171608/1‑150 (MQ=255)
                                                                                                            aGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCGTTAATACGACCCAGCAAGGGGATGTGTATACCattatt                                    >  2:217549/1‑150 (MQ=255)
                                                                                                             gTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc                                   <  2:260275/150‑1 (MQ=255)
                                                                                                              tACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTTCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTcc                                  <  1:1445298/150‑1 (MQ=255)
                                                                                                                 tAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                               <  2:946847/150‑1 (MQ=255)
                                                                                                                  aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                              <  1:1731031/150‑1 (MQ=255)
                                                                                                                  aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                              >  1:1601425/1‑150 (MQ=255)
                                                                                                                  aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTACTTGCGGCACTTTACATTAAGATAACCGTTAATAAGACCCAGCAAGGGGATGTGTAAAACATTATTACTGaa                              >  2:777684/1‑150 (MQ=255)
                                                                                                                   aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAg                             <  1:545756/150‑1 (MQ=255)
                                                                                                                       atcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAc                         >  1:1358018/1‑150 (MQ=255)
                                                                                                                       atcatTTGTAGTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATAGGAATACGGTATTACTCCGGGCGGCCCTTTCCAGGCCGATTACCTTTAATACGACCCAGCAAGGGGCTGTGTATCCCATTATTCCTGAAGGCAc                         >  1:103126/1‑150 (MQ=255)
                                                                                                                         catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActc                       >  1:698649/1‑150 (MQ=255)
                                                                                                                         catTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAAAACGACCCAGAAAGGGGATGTCTATACCATTATTCCTGAAGTCaata                       >  1:561586/1‑147 (MQ=255)
                                                                                                                             tGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTAc                   >  2:410059/1‑150 (MQ=255)
                                                                                                                             tGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTAc                   >  2:417148/1‑150 (MQ=255)
                                                                                                                                cgaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                >  1:1237776/8‑150 (MQ=255)
                                                                                                                                aTTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                >  2:1675469/1‑150 (MQ=255)
                                                                                                                                  tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCGAGTCAGATAACATTTAATACGACCCAGGAAGGGGATGTGTATACGATTATTCCTGAAGTCAATCTTACTCAAt              >  2:156058/1‑150 (MQ=255)
                                                                                                                                  tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAt              >  2:1257317/1‑150 (MQ=255)
                                                                                                                                  tACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAt              >  1:1557340/1‑150 (MQ=255)
                                                                                                                                    cAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGATGTCACTCTTACTCAATcg            >  1:1732338/1‑149 (MQ=255)
                                                                                                                                      gAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTg          <  2:1397508/150‑1 (MQ=255)
                                                                                                                                       aaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGt         >  2:789059/1‑150 (MQ=255)
                                                                                                                                         aCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt       <  1:1039114/150‑1 (MQ=255)
                                                                                                                                           aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTTTTCCTGAAGTCTATCTTACTCAATCTTGTCtat     >  1:1099394/1‑148 (MQ=255)
                                                                                                                                           aGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgt     <  2:25189/150‑1 (MQ=255)
                                                                                                                                            gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGTGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg    >  1:96013/1‑150 (MQ=255)
                                                                                                                                            gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg    <  2:1219135/150‑1 (MQ=255)
                                                                                                                                             ggCGCATGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc   <  2:1706685/150‑1 (MQ=255)
                                                                                                                                             ggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc   >  2:496330/1‑150 (MQ=255)
                                                                                                                                             ggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc   <  2:1696773/150‑1 (MQ=255)
                                                                                                                                              gcgcAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCa  <  1:990830/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCA  >  NC_000913/1104787‑1105078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: