Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,552,191 | 0 | C | A | 25.9% | 90.5 / 18.2 | 58 | intergenic (+200/+207) | fdnI/yddM | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base C (14/29); new base A (9/6); total (23/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.42e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
ATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAG > NC_000913/1552042‑1552335 | atatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCTTGAACGCCTTATCCGGCATGAAAAACCTTCAAATCCATTAGTTTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc < 1:642451/150‑1 (MQ=9) atatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc < 2:1363512/150‑1 (MQ=31) tatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa < 1:1289303/150‑1 (MQ=32) tatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa < 2:584131/150‑1 (MQ=32) tatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa < 2:1079501/150‑1 (MQ=32) tAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCATTGAACGTTTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATgg < 1:195729/150‑1 (MQ=21) aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgcgc < 1:1006441/150‑1 (MQ=34) aGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGAATGAAAACCCTTCAAATCCAATAGATTGCAGGGAACGGGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGGTTGTCATCAGTTTCAAATGGCGCTGTaaaa < 1:1411531/150‑1 (MQ=255) ggCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAAg > 1:714861/1‑150 (MQ=17) gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg > 1:1364180/1‑150 (MQ=40) gCTTTGTGCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCTAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCTCTTCAGGAAATGTTGCATTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg < 2:916876/150‑1 (MQ=14) gtttGTTCATGCCGGATCGGGCTGTAACG‑CATATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg < 2:1208457/149‑1 (MQ=14) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg < 2:1098397/150‑1 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg < 2:1567045/150‑1 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTaaaaagagac > 2:1583155/1‑144 (MQ=255) gTTCATGCCGGATGCGGAGTGAACGCCTTATCGGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGAAATGTTTCGTTTGTCATCAGTTTCAATTGGCGCTGTAAAAGGCGTCa < 2:872869/150‑1 (MQ=14) tgATGAGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCATATCCAATAGATTGCAGTGAACGTGTAGTCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCatat < 1:1570028/149‑1 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCatat < 1:407016/150‑1 (MQ=255) aTGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTg < 2:1567989/150‑1 (MQ=255) gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGTGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa < 2:415738/150‑1 (MQ=255) ggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGa > 2:1028793/1‑150 (MQ=255) gcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc < 1:1409517/150‑1 (MQ=255) cgTTAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGATTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAct < 1:492392/150‑2 (MQ=255) cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACa < 1:1345059/150‑1 (MQ=255) cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACa < 1:1351108/150‑1 (MQ=255) aCGCCTTATCCGTAATGAAAACACTTCAACTCCAATAGATTGCAGTGAACGTGTAGTCCTGATAAGCGTAGCGCATCAGGAAATGTTGCGTTTTTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTa < 2:255711/150‑1 (MQ=18) cGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAACGAGACATTTTCATATTGTATACAAAGTAg > 2:1108133/1‑150 (MQ=14) aTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTg < 1:76670/150‑1 (MQ=255) tCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGt < 2:1336177/150‑1 (MQ=255) cGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAAGGAGACATTTTGATATTGTTGACAAAGAAGGCTTTGtaa > 1:955297/1‑148 (MQ=14) cGGAATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTTTAAAAGGAGTCATTTTGATATTTTATACAAAGTCTGCTTTGTTc > 1:955300/1‑150 (MQ=14) ggCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGAAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCa < 1:802904/150‑1 (MQ=255) cATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTATTATTGTATAAAAAGAAGGCTTTATACAta > 1:166378/1‑149 (MQ=14) tGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGcc < 1:425353/150‑1 (MQ=255) tGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTATAAGGAGGCATTTTAATATTGAATAAAACGTAGGCTTAGTTCATGcc > 2:378854/1‑150 (MQ=14) ttCAAATCCAATAGATTGCATTGAAAGTGTAGTCCTGATAAGCGTAGCTCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc < 2:1265026/150‑1 (MQ=25) aaaTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg < 1:1309762/150‑1 (MQ=255) tCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTAAAAGGCGACATTTTAATATTGTATACAAAGTAGGCTTTGTTCATGCCGGATGCGGCGTGaaa > 1:1487417/1‑149 (MQ=21) gCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg > 2:543936/1‑150 (MQ=255) gCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAAAGTAGGCTTTGTTCATGCCGGATGCGGCGTGATCGACTTATCCgg > 1:1591410/1‑150 (MQ=255) gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt < 2:1364180/150‑1 (MQ=255) agtgtAGGTCTGATAAGCGTAGCGCATCAGGCAATGTTGAGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaaaa < 2:1515171/149‑1 (MQ=255) tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc > 1:1422335/1‑150 (MQ=255) tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc < 1:536156/150‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGTCGCTGTAAAATGCTTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa < 2:987796/150‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTTTAAAAAGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATTCCGGATGCTGCGTGAACGCCTTATAATGCATGAAAACACTTCa > 2:878890/1‑150 (MQ=18) gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTATTATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGGGTGAAAGACTTATACGGCATGAAAACCCTTCAAATCCAATa > 1:618153/1‑150 (MQ=18) tAGCTCATCAGGAAATGTTGGGTTTGTCATAAGATTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAt < 2:316658/150‑1 (MQ=14) cttaTCATGCAATTTTGCGTTTCGAATTAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGc < 2:315507/147‑1 (MQ=14) gcATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGAGGCGTTAAAGACTTATCCGGCATGAAAACCCTTAAAATCCAATAGaattca > 1:4728/1‑145 (MQ=17) gCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATACGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACg > 2:1087206/1‑150 (MQ=255) tGTCATGAGATTAAAATGGCGCTGTAAAAGGCGGCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATa < 2:1316985/150‑1 (MQ=255) gTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa > 2:298239/1‑150 (MQ=255) tcatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAg > 2:930466/1‑150 (MQ=40) ccataaGTTTAAAATGGCGCGGTAAAAGGCGGCATTTTCAAATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGAGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAATGCAGTGAACGTGTAGGCCTGATAAg < 2:258182/149‑1 (MQ=11) catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc > 2:353746/1‑150 (MQ=40) catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc > 2:452873/1‑150 (MQ=40) catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCATTCAAATCCAATAGATTGCAGTGGACGTGTAGGCCTGATAAGc > 2:1267503/1‑150 (MQ=255) aGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg > 2:1272220/1‑150 (MQ=37) aGTTGCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg < 1:371652/150‑1 (MQ=21) | ATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAG > NC_000913/1552042‑1552335 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |