Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,473,927 C→A A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9270CA100.0% 350.4 / NA 104A43S (GCC→TCC) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (51/53);  total (51/53)

Only 100 of 104 total aligned reads displayed.

GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073
                                                                                                                                                   |                                                                                                                                                  
gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCGGTCAGCACTATCTGTGAATACTGCTGTCGCTGCAGTCTCTTGTGCCAGGCGTTTAGCGGAAt                                                                                                                                                  >  1:1301610/1‑150 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  1:647238/150‑1 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  1:960359/150‑1 (MQ=255)
      cGCCAACGAAGCCAATACCTGCATTTACCACCAGGATCTCCAATGCGCCGCTCTTACGAACGACATCAATGACCGCGTCGCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCg                                                                                                                                            <  2:250656/150‑1 (MQ=255)
      cGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCg                                                                                                                                            <  2:813495/150‑1 (MQ=255)
      cGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCGCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCg                                                                                                                                            <  1:770622/150‑1 (MQ=255)
       gCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           >  1:1107210/1‑150 (MQ=255)
        ccAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTCTGCCAGGCGTTTAGCGGAATCTTTCGAc                                                                                                                                          >  1:167844/1‑150 (MQ=255)
         cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAcc                                                                                                                                         <  1:813647/150‑1 (MQ=255)
                gCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATa                                                                                                                                  <  2:1552817/150‑1 (MQ=255)
                gCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATa                                                                                                                                  <  2:588502/150‑1 (MQ=255)
                  cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                >  1:1348299/1‑150 (MQ=255)
                       ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  2:1144585/1‑150 (MQ=255)
                        cTGCATTTACCCCCAGGATATCCAATGCGCCGCGCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGGGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          <  2:1436930/150‑1 (MQ=255)
                        cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          >  1:1424244/1‑150 (MQ=255)
                        cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATc                                                                                                                          <  1:1544135/150‑1 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  1:1037807/1‑150 (MQ=255)
                          gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGt                                                                                                                        >  1:777669/1‑150 (MQ=255)
                                accaccAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTg                                                                                                                  <  1:1282947/150‑1 (MQ=255)
                                 ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTgg                                                                                                                 <  2:141059/150‑1 (MQ=255)
                                  caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                >  1:1293653/1‑150 (MQ=255)
                                  caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                <  2:1313115/150‑1 (MQ=255)
                                  caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                                                                                                                <  1:1551670/150‑1 (MQ=255)
                                            tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                      >  2:1150719/1‑150 (MQ=255)
                                            tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                      >  1:1157903/1‑150 (MQ=255)
                                             ccAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGa                                                                                                     <  2:1073471/150‑1 (MQ=255)
                                              cAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc                                                                                                    >  2:1431473/1‑150 (MQ=255)
                                              cAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc                                                                                                    >  1:1132359/1‑150 (MQ=255)
                                              cAATGCGCCGCTCTTACGAACGACAACAATGACAGCGTCTCTGTCAGAACTATCTGTGAATACTGCTGTCGCTCCACTCACTTGTGCCAGGCGATTAACGGAATCTTTCTACCACGCAAAGGTCAATCGTACATTGGCCCAATCAGTGaa                                                                                                    >  1:202921/1‑149 (MQ=255)
                                               aaTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg                                                                                                   <  2:862296/150‑1 (MQ=255)
                                               aaTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg                                                                                                   <  2:366303/150‑1 (MQ=255)
                                               aaTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg                                                                                                   <  1:1395662/150‑1 (MQ=255)
                                               aaTGCGCCGCTCTTACGAACGACATCAATGACAGAGTCTCTGTCAGCACTATCTGGGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg                                                                                                   <  1:1321780/150‑1 (MQ=255)
                                                     ccgcTCTTACGAACGACATCAATGACAGGGTCTCTGTCATCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacga                                                                                             <  2:794221/150‑1 (MQ=255)
                                                     ccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGAGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCGCCATCGGTGACGAAacga                                                                                             >  2:885705/1‑150 (MQ=255)
                                                      cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgac                                                                                            <  2:129296/150‑1 (MQ=255)
                                                      cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgac                                                                                            <  2:422301/150‑1 (MQ=255)
                                                       gcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgacg                                                                                           <  2:1295590/150‑1 (MQ=255)
                                                          ctTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAc                                                                                        <  1:671336/150‑1 (MQ=255)
                                                            tACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAACCGACGTACGa                                                                                      >  1:529030/1‑150 (MQ=255)
                                                               gaacgaCATCAATGACAGAGTCTCTGTCAGCACTATCTGTGAATACTGCTTTCGCTCCAGTCTCTTTTGCCAGGCGTAAAGCCGAATCTTTCGCCCCCGCAGAGGAGAATCGGACATTGGACACAGCGGTGACGAAACGACCTACGCTAg                                                                                   >  2:408225/1‑150 (MQ=255)
                                                                aacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGGGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                  <  1:935856/150‑1 (MQ=255)
                                                                 acgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                 <  2:1335825/150‑1 (MQ=255)
                                                                  cgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCGCTTGTGCCCGGCGTTTAGCGGAATCTGTCGCCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGGGACGAAACGCCGGACGATAGCgg                                                                                >  1:94827/1‑150 (MQ=255)
                                                                   gaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGc                                                                               <  1:1287901/150‑1 (MQ=255)
                                                                   gaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCGTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGc                                                                               >  1:966753/1‑150 (MQ=255)
                                                                    aCATCAATGACAGCGTCTCTGTCAGCACAATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGCGACGAAACGACGTACGATAGCGGCa                                                                              <  2:677144/150‑1 (MQ=255)
                                                                      aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            <  2:1424244/150‑1 (MQ=255)
                                                                       tCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAccc                                                                           >  1:149287/1‑149 (MQ=255)
                                                                       tCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCg                                                                           >  1:1172748/1‑150 (MQ=255)
                                                                          aTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATa                                                                        <  1:414616/150‑1 (MQ=255)
                                                                             aCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCa                                                                     >  2:587440/1‑150 (MQ=255)
                                                                             aCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCa                                                                     >  2:139322/1‑150 (MQ=255)
                                                                                gCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGa                                                                  <  1:1419836/150‑1 (MQ=255)
                                                                                  gTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACt                                                                >  1:821463/1‑150 (MQ=255)
                                                                                   tctcTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTg                                                               <  2:1421469/150‑1 (MQ=255)
                                                                                    ctctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGc                                                              >  1:680636/1‑150 (MQ=255)
                                                                                     tctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAAGCTTTCGCCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGcc                                                             <  1:1409778/150‑1 (MQ=255)
                                                                                      ctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCa                                                            <  2:1499671/150‑1 (MQ=255)
                                                                                         gcAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCg                                                         <  1:1181738/149‑1 (MQ=255)
                                                                                             cACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGa                                                     >  1:1141822/1‑150 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                <  1:1396248/150‑1 (MQ=255)
                                                                                                  tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                <  1:1144585/150‑1 (MQ=255)
                                                                                                    tgtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAAc                                              >  2:1462744/1‑150 (MQ=255)
                                                                                                      tgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                            >  1:165501/1‑150 (MQ=255)
                                                                                                      tgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                            <  1:900231/150‑1 (MQ=255)
                                                                                                             tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     >  2:941423/1‑150 (MQ=255)
                                                                                                             tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     >  2:601926/1‑150 (MQ=255)
                                                                                                             tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     <  1:108298/150‑1 (MQ=255)
                                                                                                             tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACAGCCACCGAGGATGAGAACTGTCTTAcc                                     >  2:1334671/1‑150 (MQ=255)
                                                                                                             agctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGCCGCAACGACGTACGATAGCGGCACCGATACCACGCCTGCCACCGAGGATGAGAACTGTCTTAcc                                     >  2:897525/2‑150 (MQ=255)
                                                                                                              gctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                    >  2:46103/1‑150 (MQ=255)
                                                                                                                     cTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAg                             <  2:777669/150‑1 (MQ=255)
                                                                                                                       ccAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTTAAGCGTACATTGTCCCCATCGGTGACGAAACGAATTACGATAGCGGATCCGATACCACGTCTGCCACCGTGGATGAGAAGTGTCTTTCCTGTAATAgcg                           <  2:845097/150‑1 (MQ=255)
                                                                                                                        cAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgcgc                          >  2:1541110/1‑150 (MQ=255)
                                                                                                                         aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         >  1:841897/1‑150 (MQ=255)
                                                                                                                           tctcTTGTGCCAGGCGTTTAGCGGATTCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCa                       <  1:708563/150‑1 (MQ=255)
                                                                                                                           tctcTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCa                       >  1:428871/1‑150 (MQ=255)
                                                                                                                             tctTGTGCCAGGCGTTTAGCGGAATCTTTCGCCCCCGCATAGGTGAATCGTACATTGGCGCCATCGGTGACGAAACGACGTACGATTGAGGCACCGATACCACGACTGCCACCGAGGATGAGAGCTGGCTTCCCGGTAAAAGCGCCCATa                     >  2:94541/1‑150 (MQ=255)
                                                                                                                             tctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCTCGGTGCGGATCCGCCGTACGATAGCGGCGCCGATACCGCGCCTGCCACCGAGGAGGAGACCTGTCTGACCTGTAAAAGCGCCCATa                     >  2:239638/1‑150 (MQ=255)
                                                                                                                                tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                  <  2:1132359/150‑1 (MQ=255)
                                                                                                                                tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                  <  2:1107210/150‑1 (MQ=255)
                                                                                                                                tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCACATAGGTGAATTGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                  >  1:410967/1‑150 (MQ=255)
                                                                                                                                  tgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGAc                >  2:679533/1‑150 (MQ=255)
                                                                                                                                   gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACt               >  2:804450/1‑150 (MQ=255)
                                                                                                                                   gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACt               >  2:622311/1‑150 (MQ=255)
                                                                                                                                        gCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg          <  2:533841/150‑1 (MQ=255)
                                                                                                                                         cGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGCTACCACGACTGCCACCGAGGATGAGAACTCTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGa         >  2:718817/1‑150 (MQ=255)
                                                                                                                                           tttATCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAtt       <  2:1097824/150‑1 (MQ=255)
                                                                                                                                           tttAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAtt       <  2:1201606/150‑1 (MQ=255)
                                                                                                                                           tttAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAtt       <  1:949070/150‑1 (MQ=255)
                                                                                                                                             tAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATtta     <  2:821463/150‑1 (MQ=255)
                                                                                                                                              aGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGCGGCACCGCTACCACGACTGCCCCCGAGGATGAGACCTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCGGGATttat    >  1:1338658/1‑150 (MQ=255)
                                                                                                                                               gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGCACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   >  2:1490173/1‑150 (MQ=255)
                                                                                                                                               gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAATAGCGCCCATAAGGACTCCTTGATttatt   <  1:731603/150‑1 (MQ=255)
                                                                                                                                               gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   >  2:1096170/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGGCTCCTTGATttatta  >  1:836394/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  >  1:241842/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  >  2:1179442/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  <  1:1169883/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑