Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,458,110 C→A T578T (ACC→ACA)  gspD → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,1100CA100.0% 350.1 / NA 105T578T (ACC→ACA) gspDgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (57/48);  total (57/48)

Only 100 of 108 total aligned reads displayed.

ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCT  >  NC_000913/3457964‑3458253
                                                                                                                                                  |                                                                                                                                               
aCTATTCAACACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                              <  2:152257/150‑1 (MQ=255)
aCTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                              <  1:391737/150‑1 (MQ=255)
   aTTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgc                                                                                                                                           >  2:379181/1‑150 (MQ=255)
    ttCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                          <  1:405550/150‑1 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                        >  2:51949/1‑150 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                        >  1:364487/1‑150 (MQ=255)
        aaaCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGAGTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTaa                                                                                                                                      >  1:1054005/1‑150 (MQ=255)
        aaaCGCCCTGCTGGTCAAAACAGGTGAAACAGTCGTCCTGGGCGGATTGCTGGATGATTTATATAAAGAACAAGATTCAAAGGTTACTCTGCTTGGCGATATTCCTTTAGTGGGTCAACTCTTCCACTATACCTCCACATAGCGCACTaa                                                                                                                                      >  2:430040/1‑150 (MQ=255)
           cGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTTGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACg                                                                                                                                   <  2:1197241/150‑1 (MQ=255)
           cGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACg                                                                                                                                   <  1:557873/150‑1 (MQ=255)
           cGCCGTGCGGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACg                                                                                                                                   <  2:1190840/150‑1 (MQ=255)
            gCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGc                                                                                                                                  >  2:1575078/1‑150 (MQ=255)
               gTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGTCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAc                                                                                                                               <  2:67546/150‑1 (MQ=255)
                tGCTGGTCAAAACCGGTGAAACGTTGGTCGTGGGCGGATTGCTGGATGATTATTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATGCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACATAGCGCGCTAAACGCAAcc                                                                                                                              >  2:807042/1‑150 (MQ=255)
                tGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAcc                                                                                                                              <  2:600854/150‑1 (MQ=255)
                   tGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                           <  2:1020414/150‑1 (MQ=255)
                    ggTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAt                                                                                                                          <  1:227011/150‑1 (MQ=255)
                    ggTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAt                                                                                                                          <  2:390527/150‑1 (MQ=255)
                     gTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                         <  1:259732/150‑1 (MQ=255)
                      tCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATgg                                                                                                                        <  2:1230683/150‑1 (MQ=255)
                       cAAAACCGGTGAACCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAATGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGt                                                                                                                       <  2:870707/150‑1 (MQ=255)
                          aaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                    <  2:362894/150‑1 (MQ=255)
                            ccGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTACTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTa                                                                                                                  >  1:1176658/1‑150 (MQ=255)
                              ggTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtc                                                                                                                <  2:172056/150‑1 (MQ=255)
                               gTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtcc                                                                                                               >  1:733923/1‑150 (MQ=255)
                               gTGAAACGGTGGTCCTGAGCGGATTGCTGGATGATTTTTATAAAGATCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGATAAACGCAACCTTATTATATTTAtca                                                                                                               >  1:78248/1‑149 (MQ=255)
                                 gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCATCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgt                                                                                                             >  2:1296016/1‑150 (MQ=255)
                                  aaaCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGATCCACTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccttc                                                                                                            >  2:1228256/1‑150 (MQ=255)
                                     cGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcga                                                                                                         >  1:949565/1‑150 (MQ=255)
                                         ggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAt                                                                                                     <  1:1391719/150‑1 (MQ=255)
                                         ggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAt                                                                                                     >  2:693096/1‑150 (MQ=255)
                                          gtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAtt                                                                                                    >  2:868953/1‑150 (MQ=255)
                                             cTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGATTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCCCAGAGCGCGCTAACCGCAACCGGATGGTATTTATCCGTCCGACGATTATc                                                                                                 >  2:298474/1‑150 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTACACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                >  1:304894/1‑150 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                >  1:1045751/1‑150 (MQ=255)
                                                  cGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGa                                                                                            <  2:666195/150‑1 (MQ=255)
                                                   ggATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGAc                                                                                           <  1:1465727/150‑1 (MQ=255)
                                                   ggATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGAc                                                                                           <  2:447161/150‑1 (MQ=255)
                                                    gATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACg                                                                                          >  2:159154/1‑150 (MQ=255)
                                                        gCTGGATGTTTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGTGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatga                                                                                      <  1:283963/150‑1 (MQ=255)
                                                        gCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatga                                                                                      >  1:1200489/1‑150 (MQ=255)
                                                        gCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCGTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatga                                                                                      <  2:1227073/150‑1 (MQ=255)
                                                          tGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGTGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCTTCCTACGATTATCAGTGACgatgagg                                                                                    >  1:282504/1‑148 (MQ=255)
                                                          tGGATGATTTATCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGatgatg                                                                                    <  2:1555708/150‑1 (MQ=255)
                                                           ggATGATTTTTCTAAAGATCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCACCCTCATGGTATTAATCCGTCCGACGATTATCCGTGACGATGATGt                                                                                   >  2:982759/1‑150 (MQ=255)
                                                           ggATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGt                                                                                   >  1:25930/1‑150 (MQ=255)
                                                                atTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATc                                                                              >  1:338175/1‑150 (MQ=255)
                                                                      cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCACCCTGATGGTATATATCCGTCCGCGGCTTATCCGTGACGATGATGTTTATCGCTCag                                                                        >  2:222181/1‑149 (MQ=255)
                                                                      cTAAAGAGAAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCAc                                                                        <  2:17998/150‑1 (MQ=255)
                                                                       tAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCAGCTATACCTCCACAGAGAGCGCTAAACGCAACCTGATGGTATTTATCCTTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACt                                                                       >  2:1104181/1‑150 (MQ=255)
                                                                       gaaaGAGCAAGTGTCAAAGGTTACTCTGCTTGGCGATATTCCTTTAGTCGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAAGGTATTTATCCGACCGACGATTATCAGTGACGATGCTGGTTATCGCGCAcg                                                                       >  1:1011782/2‑149 (MQ=255)
                                                                        aaaGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACtt                                                                      >  1:1091845/1‑149 (MQ=255)
                                                                          agatCAAGTGTCAAAGTTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCAGGTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGTTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTc                                                                    <  1:480821/150‑1 (MQ=255)
                                                                          agagCAAGTGTCAAAGGTTCCGCTGCGTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTAGACCTCCACAGCGCGCGCTATACGCAACCTGATGGTATTTAGACGTCCGACGATTTTCCGTGACGATGATGTTTATCGCTCAGTGTc                                                                    >  2:84390/1‑150 (MQ=255)
                                                                            cgCAAGTGTCAAACGTTCCTCTGCTTGGCGATATTCCTTTAGTGGCGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGTTGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGATTATCGCTCACTGTCaa                                                                  <  2:78248/149‑1 (MQ=255)
                                                                                   gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaa                                                           >  1:843453/1‑150 (MQ=255)
                                                                                    tCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                          >  1:1360298/1‑150 (MQ=255)
                                                                                     cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAAt                                                         >  2:598979/1‑150 (MQ=255)
                                                                                     cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAAt                                                         >  1:1427446/1‑150 (MQ=255)
                                                                                      aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                        >  1:1010077/1‑150 (MQ=255)
                                                                                      aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                        >  1:1519519/1‑150 (MQ=255)
                                                                                        aGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATTCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATaca                                                      >  1:949893/1‑150 (MQ=255)
                                                                                          gTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACAcc                                                    <  1:617257/150‑1 (MQ=255)
                                                                                                ctGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTAc                                              >  2:1029820/1‑150 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTTCCg                                            >  1:583780/1‑150 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCg                                            <  2:1558628/150‑1 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGAGGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCg                                            >  1:1152621/1‑150 (MQ=255)
                                                                                                       gCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAgg                                       <  1:389360/150‑1 (MQ=255)
                                                                                                       gCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAgg                                       >  1:1315846/1‑150 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCAGTGGCAAAAGAGAAATACGCCCGTTACCGTCAGGa                                      >  2:1070641/1‑150 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGCGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                      <  2:405496/150‑1 (MQ=255)
                                                                                                          atatTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGc                                    >  1:1319161/1‑150 (MQ=255)
                                                                                                              tCCTTTAGTGGGGCAACTCTTCCGCCATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaaca                                >  2:1436017/1‑150 (MQ=255)
                                                                                                                cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCCCTGTCAAAAGAGAAATACACCCGTTACCGTAAGGAGCaacaac                              >  2:860013/1‑150 (MQ=255)
                                                                                                                cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCaacaac                              <  1:1575078/150‑1 (MQ=255)
                                                                                                                  ttAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAg                            <  2:1363403/150‑1 (MQ=255)
                                                                                                                    aGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCg                          <  1:868953/150‑1 (MQ=255)
                                                                                                                      tGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAACCGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCCACAACAGCGGa                        >  2:161111/1‑150 (MQ=255)
                                                                                                                      tGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                        <  1:657580/150‑1 (MQ=255)
                                                                                                                       ggggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGAt                       >  1:1501949/1‑150 (MQ=255)
                                                                                                                        gggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATc                      >  2:1140822/1‑150 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGGCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                     <  2:949565/150‑1 (MQ=255)
                                                                                                                           cAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGAc                   >  2:1444675/1‑150 (MQ=255)
                                                                                                                            aaCTCTTCCGCTATCCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACg                  <  2:930647/150‑1 (MQ=255)
                                                                                                                            aaCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACg                  <  2:1186573/150‑1 (MQ=255)
                                                                                                                             aCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACgg                 >  1:410744/1‑150 (MQ=255)
                                                                                                                                   ccGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATc           <  1:948510/150‑1 (MQ=255)
                                                                                                                                    cGCTATACCTCCACAGAGCGCGCTAAACTCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCa          <  2:1176658/150‑1 (MQ=255)
                                                                                                                                       tataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAATAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaaa       <  1:51949/150‑1 (MQ=255)
                                                                                                                                       tataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaaa       >  2:28693/1‑150 (MQ=255)
                                                                                                                                        ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg      <  2:1442561/150‑1 (MQ=255)
                                                                                                                                        ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg      <  2:50731/150‑1 (MQ=255)
                                                                                                                                        ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg      <  2:1010077/150‑1 (MQ=255)
                                                                                                                                        ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg      <  2:1054005/150‑1 (MQ=255)
                                                                                                                                        ataCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg      <  2:1519519/150‑1 (MQ=255)
                                                                                                                                         taCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgc     >  1:100684/1‑150 (MQ=255)
                                                                                                                                         taCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGATCACTGTCAAAAGAGAAATACACCAGTTAACGTCAGGAGCAACAACAGCGGATATACGGGAAATCAAAAgc     >  2:925224/1‑150 (MQ=255)
                                                                                                                                          aCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcg    >  2:973788/1‑150 (MQ=255)
                                                                                                                                           ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcgc   >  2:829366/1‑150 (MQ=255)
                                                                                                                                            cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAGCAACAGCGGATCGACGGGAAATCAAAAgcgcg  >  1:199949/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCT  >  NC_000913/3457964‑3458253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑