Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 293:1 +G 5.2% intergenic (+38/‑44) thrL → / → thrA thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132931.G 5.2% 2060.8 / 101.2 446intergenic (+38/‑44)thrL/thrAthr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base . (195/228);  new base G (12/11);  total (207/239)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 459 total aligned reads displayed.

AGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG  >  NC_000913/144‑442
                                                                                                                                                      |                                                                                                                                                      
aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa                                                                                                                                                         >  2:2199368/1‑150 (MQ=255)
aGGCAAAGCGCACAGACAGATAAAAAATACAGAGAACACAACATCCATGAAACGCATTAGCACCACCAAAACCACCACCATCACCATAACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTcaca                                                                                                                                                         >  2:1651365/1‑150 (MQ=255)
     tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCg                                                                                                                                                   <  2:4413192/150‑1 (MQ=255)
      aGCGCACATACATATAAAAATTACAGAGTACACAACATCCATTAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAAGGGTGCGGGCTGACGCGTACAGGAAACACATAAAAAAGCCCGCACCTGACAGTGCgg                                                                                                                                                  <  1:505467/150‑1 (MQ=255)
      aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGACACACAGAAAAAAGCCCGCACCTGACAGTGCgg                                                                                                                                                  >  2:1815969/1‑150 (MQ=255)
        cgcAAAGACAGATAAAAATTACAGATTACACACACTACATGAAACGATTTAGCACCAGCAGTACCACCACCATAACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACTGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGc                                                                                                                                                <  1:7112780/150‑1 (MQ=255)
         gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                                                                              >  2:7759558/1‑150 (MQ=255)
          cacaGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCCCCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtt                                                                                                                                             <  1:1145728/150‑1 (MQ=255)
            cagacagaTAAAAATTACAGAGGACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACCGAAAAAAGCCCGCACCTGACAGTGCGGGCtttt                                                                                                                                           <  2:4956859/150‑1 (MQ=255)
               acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt                                                                                                                                        >  1:3772488/1‑150 (MQ=255)
               acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt                                                                                                                                        <  1:5690489/150‑1 (MQ=255)
                  taTAAAAATTACAGATTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGGTAAGGGTGCGGGCTGACGCTTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTc                                                                                                                                     <  1:1826353/149‑1 (MQ=255)
                    tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCCCCATTACCCCCACCAACACCATTACCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACACAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgtgt                                                                                                                                   <  1:2161777/150‑7 (MQ=37)
                    tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGa                                                                                                                                   <  2:5742779/150‑1 (MQ=255)
                    aaaaaaTGACAGATTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCCCTCGTAACGGTGCGGGCTCACGCGTACAGTAAACACCCAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGa                                                                                                                                   <  1:8618735/149‑1 (MQ=255)
                     aaaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCCCCAACACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACATAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAc                                                                                                                                  <  1:6204918/150‑1 (MQ=255)
                     aaaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAc                                                                                                                                  <  1:8945556/150‑1 (MQ=255)
                         aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaaa                                                                                                                              <  2:6493068/150‑1 (MQ=255)
                               gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAATAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAAc                                                                                                                        >  1:345097/1‑150 (MQ=255)
                                  tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAg                                                                                                                     <  1:7340564/150‑1 (MQ=255)
                                       aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACAATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaac                                                                                                                <  1:5099193/150‑1 (MQ=255)
                                         caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa                                                                                                              >  1:3143740/1‑150 (MQ=255)
                                           tCCATGAAACGCATTAGCACCACCATTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAAcc                                                                                                            <  1:1678679/150‑1 (MQ=255)
                                            ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                                                                           >  2:7308091/1‑150 (MQ=255)
                                              aTGAAACGCATAAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg                                                                                                         >  1:7531503/1‑150 (MQ=255)
                                               tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc                                                                                                        >  1:8577704/1‑150 (MQ=255)
                                                 aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa                                                                                                      <  1:8766955/150‑1 (MQ=255)
                                                 aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa                                                                                                      >  2:3853482/1‑150 (MQ=255)
                                                    cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt                                                                                                    <  2:7136188/150‑1 (MQ=255)
                                                        ttAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa                                                                                               >  1:1713869/1‑150 (MQ=255)
                                                          aGCACCACCATTACTACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg                                                                                             >  2:1363250/1‑150 (MQ=255)
                                                          aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg                                                                                             >  1:9176689/1‑150 (MQ=255)
                                                             atcaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTGTGTTCGACCACAGGTAACGAGGTAACACCCCTGCGCGGGTTGAAGTTc                                                                                          >  2:7676071/3‑150 (MQ=255)
                                                             accaccATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                                                                          <  1:5550461/150‑1 (MQ=255)
                                                               caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                                                                        >  1:8707048/1‑150 (MQ=255)
                                                                accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                                                                       <  1:3902905/150‑1 (MQ=255)
                                                                accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                                                                       <  1:5444321/150‑1 (MQ=255)
                                                                 ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcg                                                                                      <  2:3488468/150‑1 (MQ=255)
                                                                    ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCTTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa                                                                                   <  1:7411994/150‑1 (MQ=255)
                                                                    ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa                                                                                   <  2:8715671/150‑1 (MQ=255)
                                                                     tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc                                                                                  <  2:3772488/150‑1 (MQ=255)
                                                                       ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                                                                                >  1:6913945/1‑150 (MQ=255)
                                                                        caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc                                                                               >  2:8073253/1‑150 (MQ=255)
                                                                        caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc                                                                               >  2:3250996/1‑150 (MQ=255)
                                                                         accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAATAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGTCCAATGGTAACGTGGTTACAACCTTTCGTGTTTTGTAGTTAGGCGGTACAtga                                                                              >  2:1597274/1‑148 (MQ=255)
                                                                           caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGt                                                                            <  2:7531503/150‑1 (MQ=255)
                                                                           caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGACCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGt                                                                            >  1:6416553/1‑150 (MQ=255)
                                                                            accatcaccatTACCACAGGTAACGGTGCAGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                                                                           >  2:6176613/1‑150 (MQ=255)
                                                                                tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaa                                                                       >  1:1226655/1‑150 (MQ=255)
                                                                                 caccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaaa                                                                      >  2:5011634/1‑150 (MQ=255)
                                                                                  accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAAt                                                                     <  1:7743402/150‑1 (MQ=255)
                                                                                   ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg                                                                    <  1:2287708/150‑1 (MQ=255)
                                                                                    catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc                                                                   >  1:5783762/1‑150 (MQ=255)
                                                                                    catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc                                                                   >  1:1069603/1‑150 (MQ=255)
                                                                                     atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa                                                                  <  1:4504181/150‑1 (MQ=255)
                                                                                      ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAg                                                                 >  2:7997644/1‑150 (MQ=255)
                                                                                             aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                          >  2:8980490/1‑150 (MQ=255)
                                                                                               gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc                                                        <  1:5291604/150‑1 (MQ=255)
                                                                                               gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCCAGTGGTGAAGGTCGGCGGTACCTCGGTGGCAAATGCAGAACGGTTTc                                                        >  2:8625633/1‑150 (MQ=255)
                                                                                                tAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCt                                                       <  2:524434/150‑1 (MQ=255)
                                                                                                  aCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCCAAGGTAACGAGGTAACAACCATGCGAGTGGTGAAGTTCGGCGGGACATCAGTGGCAAATGCAGAACGTTTTCTGc                                                     >  1:2448682/1‑150 (MQ=255)
                                                                                                    ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt                                                   <  1:8156460/150‑1 (MQ=255)
                                                                                                    ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt                                                   <  2:2565634/150‑1 (MQ=255)
                                                                                                    ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt                                                   <  2:2975066/150‑1 (MQ=255)
                                                                                                     gTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtg                                                  >  2:5476153/1‑150 (MQ=255)
                                                                                                        cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTg                                               <  2:2347097/150‑1 (MQ=255)
                                                                                                          ggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGcc                                             <  1:830634/150‑1 (MQ=255)
                                                                                                                 gcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc                                      >  1:4944049/1‑150 (MQ=255)
                                                                                                                   gTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg                                    <  1:9318743/150‑1 (MQ=255)
                                                                                                                    tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGGGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAAGTTTTCTGCGTGTTGCCGATATTCTgg                                   >  1:6018143/1‑150 (MQ=255)
                                                                                                                    tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg                                   >  1:402385/1‑150 (MQ=255)
                                                                                                                     aCAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa                                  <  2:3143785/150‑1 (MQ=255)
                                                                                                                       aGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa                                <  1:9096746/150‑1 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACCTCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:4640355/1‑150 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:2814173/1‑150 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:4338622/1‑150 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:1533051/1‑150 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:2723566/1‑150 (MQ=255)
                                                                                                                         gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAAAGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                              >  2:1219868/1‑150 (MQ=255)
                                                                                                                            acacAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAAt                           <  1:6697739/150‑1 (MQ=255)
                                                                                                                              acaGAAAAAAGCCCGCACCTGACAGTGCGGGCTTCTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc                         <  1:7976596/150‑1 (MQ=255)
                                                                                                                                aGAACAAAGCCCGCCCCTGACAGTGCGGGCTTTGTTTTTCGACCACAGGTACCGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCCAATGCAGACCGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       <  1:7676071/150‑1 (MQ=255)
                                                                                                                                aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCGAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       <  1:2640443/150‑1 (MQ=255)
                                                                                                                                aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       <  2:6109046/150‑1 (MQ=255)
                                                                                                                                aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       <  2:9141337/150‑1 (MQ=255)
                                                                                                                                aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAATGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       >  2:6882585/1‑150 (MQ=255)
                                                                                                                                aGAAAAAAGCCCGCACCTGACAGTGCGGGCATTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca                       <  2:8253880/150‑1 (MQ=255)
                                                                                                                                  aaaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg                     <  1:4260903/150‑1 (MQ=255)
                                                                                                                                   aaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAAAAAACAAGCGAGTGTTGAATTTAGGCGGTACATCAGTGGCAAATGCAGAACGATTTCTGCGTGTTTCCGATATTCTGGAAAGCAATGCcaggc                    >  1:4828758/1‑150 (MQ=255)
                                                                                                                                      aaGCCCGCACTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg                 >  2:4113144/1‑150 (MQ=255)
                                                                                                                                      aaGCCCGCACTGTCAGGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAAAAACCAAGCGAGTGTTGAAGTTCGGCGGTACATCAGTTGCAAATTCAGAACGTTTTGTGCGTGTTGCCGATATGATTGAAAGCAAAGCcaggcagg                 >  1:7853873/1‑150 (MQ=255)
                                                                                                                                       aGCCCGCACTGTCAGGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg               >  2:8491850/1‑150 (MQ=255)
                                                                                                                                          ccGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGGAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACATCAGTGGCCAATTCAGAACGTTTTATGCGTGTTGCAGATATTATGGAAAGCAATGCCAggcaggggca             >  1:8408683/1‑150 (MQ=255)
                                                                                                                                           cGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag            <  2:5166052/150‑1 (MQ=255)
                                                                                                                                           cGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag            <  1:5889365/150‑1 (MQ=255)
                                                                                                                                             cACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt          <  1:9055229/150‑1 (MQ=255)
                                                                                                                                              aCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg         <  1:3215531/150‑1 (MQ=255)
                                                                                                                                                cTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGc       <  2:4778282/150‑1 (MQ=255)
                                                                                                                                                cTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGc       >  1:7192357/1‑150 (MQ=255)
                                                                                                                                                 tGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGcc      >  1:1435836/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG  >  NC_000913/144‑442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: