Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,596,781 A→T 5.6% K264N (AAA→AAT lgoD → L‑galactonate oxidoreductase; L‑gulonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,596,7810AT 5.6% 1562.4 / 4.2 504K264N (AAA→AATlgoDL‑galactonate oxidoreductase; L‑gulonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (285/135);  new base T (3/43);  total (288/216)
Fisher's exact test for biased strand distribution p-value = 1.53e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 510 total aligned reads displayed.

GCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTA  >  NC_000913/4596632‑4596930
                                                                                                                                                     |                                                                                                                                                     
   gAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGt                                                                                                                                                    >  1:7741348/1‑150 (MQ=255)
   gAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGt                                                                                                                                                    >  1:2835816/1‑150 (MQ=255)
   gAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGAGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGGATTTGTCGGCCTGTTTAAAGGt                                                                                                                                                    >  1:7456962/1‑150 (MQ=255)
    aaGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTg                                                                                                                                                   >  1:4035049/1‑150 (MQ=255)
    aaGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTg                                                                                                                                                   >  1:8514946/1‑150 (MQ=255)
      gATTTTGACGCGCAGCTACGGGCGCAGTTTGTTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGTCGCCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAg                                                                                                                                                 >  2:8083063/1‑150 (MQ=255)
           tGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCa                                                                                                                                            <  2:6043315/150‑1 (MQ=255)
           tGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCa                                                                                                                                            >  1:5312810/1‑150 (MQ=255)
              cgcgCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGtt                                                                                                                                         <  1:8079541/150‑1 (MQ=255)
              cgcgCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGtt                                                                                                                                         <  2:2788134/150‑1 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAGTCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAATGGTGAGTTGCAGTtc                                                                                                                                        >  2:8747729/1‑150 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtc                                                                                                                                        >  2:7083837/1‑150 (MQ=255)
                 gcAGCTACGGGCGCTGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTTTTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtctc                                                                                                                                      <  1:5977701/150‑1 (MQ=255)
                 gcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtctc                                                                                                                                      <  1:2345100/150‑1 (MQ=255)
                 gcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtctc                                                                                                                                      <  1:2667324/150‑1 (MQ=255)
                   aGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCg                                                                                                                                    >  1:3659803/1‑150 (MQ=255)
                      tACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATTAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATc                                                                                                                                 <  2:7966903/150‑1 (MQ=255)
                        cGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCg                                                                                                                               >  2:5799682/1‑150 (MQ=255)
                        cGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCAATCCg                                                                                                                               <  2:7294867/150‑1 (MQ=255)
                         gggCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCgg                                                                                                                              >  2:917859/1‑150 (MQ=255)
                           gcgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGaa                                                                                                                            >  1:9001709/1‑150 (MQ=255)
                           gcgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGaa                                                                                                                            <  2:346166/150‑1 (MQ=255)
                            cgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAATGGTGAGTTGCAGTTCTCCGATCCGGAAt                                                                                                                           >  2:1051656/1‑150 (MQ=255)
                            cgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAt                                                                                                                           >  2:7484587/1‑150 (MQ=255)
                            cgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAt                                                                                                                           <  1:2920359/150‑1 (MQ=255)
                             gcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAtt                                                                                                                          >  1:8406472/1‑150 (MQ=255)
                                gTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCa                                                                                                                       <  2:5420197/150‑1 (MQ=255)
                                 tttGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCAt                                                                                                                      <  1:7904274/150‑1 (MQ=255)
                                 tttGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCAt                                                                                                                      >  1:1417971/1‑150 (MQ=255)
                                   tggtggTTCGCTGGCGCAGAAAGAGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaa                                                                                                                    <  2:4422182/150‑1 (MQ=255)
                                    ggtggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaa                                                                                                                   <  2:3066503/150‑1 (MQ=255)
                                     gtggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaa                                                                                                                  >  1:3864734/1‑150 (MQ=255)
                                       ggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaaaa                                                                                                                <  1:3588818/150‑1 (MQ=255)
                                        gtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAg                                                                                                               >  2:2170572/1‑150 (MQ=255)
                                        gtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAg                                                                                                               >  1:123212/1‑150 (MQ=255)
                                        gtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAg                                                                                                               >  2:6561008/1‑150 (MQ=255)
                                         ttCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGa                                                                                                              >  1:7264302/1‑150 (MQ=255)
                                          tCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGaa                                                                                                             <  2:4125972/150‑1 (MQ=255)
                                           cGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGaaa                                                                                                            >  2:3635168/1‑150 (MQ=255)
                                            gCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCCCCGATCCGGAATTCCATAAAAAAGAAac                                                                                                           >  2:7633216/1‑150 (MQ=255)
                                              tGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAacga                                                                                                         >  2:1253422/1‑150 (MQ=255)
                                                    aGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACgatga                                                                                                   >  1:8056948/1‑150 (MQ=255)
                                                    aGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACgatga                                                                                                   >  1:6555423/1‑150 (MQ=255)
                                                    aGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACgatga                                                                                                   >  1:6397614/1‑150 (MQ=255)
                                                          tGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCa                                                                                             >  2:7746988/1‑150 (MQ=255)
                                                            aTCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGc                                                                                           >  1:6320683/1‑150 (MQ=255)
                                                             tCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGcc                                                                                          >  2:8561514/1‑150 (MQ=255)
                                                             tCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGcc                                                                                          >  1:1568606/1‑150 (MQ=255)
                                                                aCGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCa                                                                                       >  1:4562339/1‑150 (MQ=255)
                                                                aCGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCa                                                                                       >  2:8820837/1‑150 (MQ=255)
                                                                   cgACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacg                                                                                    >  1:1825684/1‑150 (MQ=255)
                                                                       aGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGAGCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccac                                                                                >  2:3290059/1‑150 (MQ=255)
                                                                           aaTCAACATGCGATGAATAACACCGTGAATTTGATTCGTCAGGGCGGCACGGTGGTATTTGTCGGCCTTTTTAACGGTGTGTTGCTGTTCTCCGATCCGGAATTCAATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCg                                                                            <  2:520653/150‑1 (MQ=255)
                                                                               aaCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAg                                                                        >  1:7036412/1‑150 (MQ=255)
                                                                                  aTGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAtt                                                                     >  1:7958438/1‑150 (MQ=255)
                                                                                     cGATGAATAACACCGTTATTTTGATTCGTCAGGGCGGCAGGGTGGTATTTGTCGGCCTGTTTAAAGGTGTGTTGCTGTTCTCCGATCCGGATTTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTg                                                                  <  2:8353952/150‑1 (MQ=255)
                                                                                          catcaccccGTGAATTTGATTCGTCACGGCGGACCGGTGGTATTTGTCGGCCTTTTTAAAGGTGAGTTGCTGTTCTCCGATCCGGATTTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTaaa                                                             <  2:8268060/143‑1 (MQ=255)
                                                                                            tacccccGTGAATTTGTTGCGTCCGGGGGGCCGGGTGGTATTTGTCGGCCTGTTTAACGGTGTGTTGCTGTTCTCCGATCGGGATTTCCATAAAAAAGAAACGCCGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgt                                                           <  2:3783444/145‑1 (MQ=255)
                                                                                             aaCACCGTGAATTTGATTCGTCACGGCGGCACGGGGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGACTTCCATAAAAAAGAAACGACGATGAGGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtc                                                          >  1:5425377/1‑150 (MQ=255)
                                                                                                acCGTTAATTTGTTTCGTCACGGCGGCAGGGTGGTTTTTGTCGGCCTTTTTAACGGTGTGTTGCAGTTCTCCGATCGGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                       <  2:4993082/150‑1 (MQ=255)
                                                                                                acCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                       >  1:6712577/1‑150 (MQ=255)
                                                                                                acCGTGAATTTGATTAGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                       >  2:5946202/1‑150 (MQ=255)
                                                                                                  cGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggtcg                                                     >  1:6073840/1‑150 (MQ=255)
                                                                                                  cGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggtcg                                                     >  1:3106299/1‑150 (MQ=255)
                                                                                                   gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGa                                                    >  2:1716690/1‑150 (MQ=255)
                                                                                                   gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGa                                                    >  1:8860717/1‑150 (MQ=255)
                                                                                                   gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGa                                                    >  2:5561079/1‑150 (MQ=255)
                                                                                                   gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGa                                                    >  1:2555961/1‑150 (MQ=255)
                                                                                                    tGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGAc                                                   >  2:1569415/1‑150 (MQ=255)
                                                                                                    tGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGAc                                                   >  2:1910065/1‑150 (MQ=255)
                                                                                                     gattttGCTGCGTCACGGCGGCACGGTGTTATTTGCCGGCCTGTTTAACGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATCAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACt                                                  <  2:4670752/147‑1 (MQ=255)
                                                                                                           gATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGc                                            >  1:1943579/1‑150 (MQ=255)
                                                                                                              tCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCgga                                         >  2:693381/1‑150 (MQ=255)
                                                                                                               ggacagggcggcCGGGTGTTTTTTGTGGGCTTTTTTAATGGTGTGTTGCTTTTCTCCTACCCGGATTTCAATAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaa                                        <  1:479163/144‑1 (MQ=255)
                                                                                                                gccACGGCGGCAGGGTGTTTTTTGTCGGCCTTTTTAAGGGTTTGTTGCTTTTCCCCGACCCGGAATTCAATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaag                                       <  2:6353044/148‑1 (MQ=255)
                                                                                                                gTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaag                                       >  2:3917232/1‑150 (MQ=255)
                                                                                                                 tCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCggaagg                                      >  2:6751249/1‑150 (MQ=255)
                                                                                                                    cggcggCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGaaa                                   >  1:331559/1‑150 (MQ=255)
                                                                                                                      ggggcAGGGTGGTTTTTGTGGGCCTTTTTATTGTTGTGTTGCATTCCGCGGTACCGTAATTCCAAAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGaaaaa                                 <  1:5267196/148‑1 (MQ=255)
                                                                                                                      gcggcACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGaaaaa                                 >  1:6944095/1‑150 (MQ=255)
                                                                                                                       gggcAGGGTGGTTTTTGTGGGCGTTTTTAACGGTGCGTTGCTGTTCTCCGATCCGTAATTCCATAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                <  1:8342807/149‑1 (MQ=255)
                                                                                                                          cACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCAc                             >  2:4853984/1‑150 (MQ=255)
                                                                                                                          cACGGTGGTATTTGACGCCCTGTTTAAAGGTGAGTTGCAGTACTCCCATCCGGAATTCCATAAAAAATACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCAc                             <  2:6155409/150‑1 (MQ=255)
                                                                                                                           aCGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCAct                            >  1:1716589/1‑150 (MQ=255)
                                                                                                                            cGGTGGTTTTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCTATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActg                           <  2:569091/150‑1 (MQ=255)
                                                                                                                            cGGTGGTATTTGTCGGCCTTTTTAATGGTGAGTTGCAGTTCTCCGATCGGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActg                           <  2:2296107/150‑1 (MQ=255)
                                                                                                                                  tATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                     >  1:8695711/1‑150 (MQ=255)
                                                                                                                                     ttGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatga                  >  2:2539389/1‑150 (MQ=255)
                                                                                                                                      tGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                 >  1:4997952/1‑150 (MQ=255)
                                                                                                                                          ggCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGGAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGTTGGCGGAAGGAAAATTCACTGCTGACATGATGTTa             >  1:9307718/1‑150 (MQ=255)
                                                                                                                                           gCCTTTTTAAAGGTGAGTTGCAGTTCTCCTATCCGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTaa            <  2:1417971/150‑1 (MQ=255)
                                                                                                                                           gCCTGTTTAACGGTGCGTTGCAGTTCTCCGATCCTTAATTCAAAAAAAAATACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTaa            <  1:1019910/150‑1 (MQ=255)
                                                                                                                                           gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTaa            >  1:2089066/1‑150 (MQ=255)
                                                                                                                                           gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTaa            >  1:5727562/1‑150 (MQ=255)
                                                                                                                                            cttttttAATGGTGTGTTGCTTTTCACCTAGCCTCAATACCTAAAAAAACACACGACGATGCTGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc           <  1:4237314/146‑1 (MQ=255)
                                                                                                                                            ccTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc           >  2:7904724/1‑150 (MQ=255)
                                                                                                                                              tGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAcac         >  2:9015355/1‑148 (MQ=255)
                                                                                                                                                 ttAAAGGTGCGTTGCAGTTCTCCGATCGGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATc      <  2:5340809/150‑1 (MQ=255)
                                                                                                                                                 ttAAAGGTGAGTTGCAGTTCTCCGATCCGTAATTCCATAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATc      <  2:2962672/150‑1 (MQ=255)
                                                                                                                                                  tAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCg     >  2:4561183/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTA  >  NC_000913/4596632‑4596930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: