Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 518,334 T→G 5.8% intergenic (‑55/+6) ybbN ← / ← ybbO DnaK co‑chaperone, thioredoxin‑like protein/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913518,3340TG 5.8% 1662.8 / 7.9 568intergenic (‑55/+6)ybbN/ybbODnaK co‑chaperone, thioredoxin‑like protein/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (226/283);  new base G (47/2);  total (283/285)
Fisher's exact test for biased strand distribution p-value = 1.37e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 589 total aligned reads displayed.

ACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTC  >  NC_000913/518186‑518483
                                                                                                                                                    |                                                                                                                                                      
  cAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCa                                                                                                                                                     <  1:248773/150‑1 (MQ=255)
  cAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGGCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCGTCa                                                                                                                                                     >  2:7173622/1‑150 (MQ=255)
  cAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGGTGTCGATATTTTCTTTTACATGGGGGCTTAAGCGCGCGCGTcc                                                                                                                                                     >  1:3687369/1‑149 (MQ=255)
   aaaaaTAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCaa                                                                                                                                                    >  2:4936940/1‑150 (MQ=255)
    aaaaTAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAAc                                                                                                                                                   <  2:6474459/150‑1 (MQ=255)
      aaTAGAACAGCACCGGAGTGGTCATCGACAGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTc                                                                                                                                                 >  1:7513599/1‑150 (MQ=255)
       aTAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTGGGTATTTTGTTTTACATGGGGGCTTAAGCGCGCGCGTCAACTcc                                                                                                                                                >  1:6346881/1‑149 (MQ=255)
          gAACAGCACCGGAGTGGTCATCGACTGTTCTAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCAccc                                                                                                                                            <  2:2673176/150‑1 (MQ=255)
            aCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTGGGTATTTTGTTTGTCATGGGGGCTTAAGCGCGCGCTTCAACTCAccccg                                                                                                                                          >  1:1172706/1‑149 (MQ=255)
             cAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTATTTTACATGGGGGCTTAAGCGCGCGCTTAAACACACCCCTg                                                                                                                                         >  2:1844400/1‑150 (MQ=255)
                cACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGGTGTCGATATTTTCTTTGACATTGGGGCGTAAGCGCGCGCTTCAACACACCCCGGCaa                                                                                                                                      >  1:9204536/1‑150 (MQ=255)
                cACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCGGTTGTCGATATTTTCTTTTAAATGGGGGCGTAAGCGCGCGCTTCAAAGCACCCCAGCac                                                                                                                                      >  1:2464444/1‑149 (MQ=255)
                        tGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGt                                                                                                                              <  2:6972028/150‑1 (MQ=255)
                        tGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGt                                                                                                                              <  1:4264075/150‑1 (MQ=255)
                        tGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGt                                                                                                                              <  1:8776927/150‑1 (MQ=255)
                          gTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTGTATGTTTGCGATATTTTAATTGTCAATGGGGCTTAAGCGCGCCCATAAACTCACCCCGGCAAAAatttttac                                                                                                                            >  1:1745164/1‑146 (MQ=255)
                          gTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTcc                                                                                                                            <  2:2031542/150‑1 (MQ=255)
                             aTCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACAAGGGGGCTTAAGCGCGCGCGTCAACACACCCCAGCAAAATTTTGTCCaac                                                                                                                         >  1:337279/1‑148 (MQ=255)
                              tCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTTACATGGGGGCTTAAGCGCGCGCTTAAACACACCCCTGCAAAATTTTGTCCAtaa                                                                                                                        >  1:4999343/1‑148 (MQ=255)
                              tCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCa                                                                                                                        <  2:9283580/150‑1 (MQ=255)
                                gACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCAcg                                                                                                                      >  2:9086423/1‑150 (MQ=255)
                                gACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTAGATATTTTCTTTGAAATGGGGGCTTAATCGCGCGCTTCAAAAAACCCCAGCAAAATTTTTTACATCaag                                                                                                                      >  2:3495505/1‑148 (MQ=255)
                                 aCTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCAcgc                                                                                                                     <  1:26291/150‑1 (MQ=255)
                                       cAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCg                                                                                                               <  2:5356223/150‑1 (MQ=255)
                                        aaGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTGTCTGTTGTCGATATTTTATTTTTCATTGGGGCTTAATCGCCCGCGTCAACACACCCCAGCCAAATTTTTTACAACACGcccccccg                                                                                                              >  1:2059311/1‑148 (MQ=255)
                                        aaGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGGTGTGGATATTTTGTTTTTCATGGGGGCGTAAGCGCGCCCGTCAACTCACCCCGGCAATATTTTGTCCATCACGCGcccccg                                                                                                              >  1:2295642/1‑148 (MQ=255)
                                         aGAAACTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCTATATTTTGTCCATCACGCGCCCAGgc                                                                                                             <  2:2936445/150‑1 (MQ=255)
                                          gAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCAACCCTGCAATATATTGTACATCACGCGCCCCCgca                                                                                                            >  2:7378995/1‑150 (MQ=255)
                                                 ttGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGgcg                                                                                                     <  2:5181060/150‑1 (MQ=255)
                                                  tGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTGTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGgcgc                                                                                                    <  1:7044552/150‑1 (MQ=255)
                                                  tGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGgcgc                                                                                                    <  2:5925687/150‑1 (MQ=255)
                                                   gCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCt                                                                                                   <  2:387932/150‑1 (MQ=255)
                                                   gCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCt                                                                                                   <  1:4082861/150‑1 (MQ=255)
                                                    cAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCtt                                                                                                  >  2:4535335/1‑150 (MQ=255)
                                                     aGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCATTTAAATTGGGGCTTAAGCGCGCGCATCAAATCAACCCTGCAAAATTTTTTACATCACGCGCCCCCGCAGCAGGCGCTTa                                                                                                 >  2:1772893/1‑150 (MQ=255)
                                                      ggTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTaa                                                                                                <  1:6200337/150‑1 (MQ=255)
                                                       gTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGAAATTTTATTTTTCAATGGGGGTTAATCGCGCGCGTCAAAAAACCCCAGCAAAAATTTTTACAACAACCGCCCCCCCACCAGGCGCATAAg                                                                                               >  1:5114086/1‑150 (MQ=255)
                                                        ttAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACACACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGc                                                                                              >  2:5765852/1‑150 (MQ=255)
                                                            aTTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCa                                                                                          <  1:4890428/150‑1 (MQ=255)
                                                                gTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTGTGTTGTCGATATTTTATTTTACATGGGGGCGTTATAGCCCGCATCAACACAACCCCGCCAAAATTTTTACCACAAGCGCCCCCGCCCCACGCGCGTAAGCAcccaaac                                                                                      >  1:5664061/1‑145 (MQ=255)
                                                                gTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTATCGGTTGTCGATATTTTCTTTGACAAGGGGGCTTAAGCGCCCGCGTCAACACAACCCTGCAATATTTTGTCCATAACGCGCCCCGGCACCAGGCGCGTAATCAcccatac                                                                                      >  2:7895420/1‑145 (MQ=255)
                                                                 ttAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCGGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTAcc                                                                                     >  2:2112848/1‑150 (MQ=255)
                                                                    aTGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGcc                                                                                  <  2:3726939/150‑1 (MQ=255)
                                                                      gTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCa                                                                                <  2:4648181/150‑1 (MQ=255)
                                                                      gTTGACAATATTTTCTACGGACATGGAGTCGCTCTATTTTTTCGATATTTTATTTTAAAATGGGGCTTAATCGCGCGCGTAAAAACAACCCAGCAAAATATTTTGCAAAAACCCCCCCCGCCGCAGGCGCGTAAGCACCAATAACGcccc                                                                                >  1:6694838/1‑149 (MQ=255)
                                                                        tGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAgg                                                                              <  1:8955371/150‑1 (MQ=255)
                                                                           cAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCa                                                                           >  2:7711671/1‑150 (MQ=255)
                                                                            aaTATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCAc                                                                          <  2:8492817/150‑1 (MQ=255)
                                                                              tatTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCa                                                                        <  2:861356/150‑1 (MQ=255)
                                                                              tatTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCa                                                                        <  1:1284685/150‑1 (MQ=255)
                                                                                ttttCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGc                                                                      <  2:7786763/150‑1 (MQ=255)
                                                                                     tACGGACATGGAGTCGCTCGCTGTTGTCGATATTTTCGTTGACATGGGGGCTTAAGCGCGCCCTTCCACGCACCCCGGCAATAATTTGTCCATCACCCCCCCCGGCAGCAGGCGCTTAAGCACCAATACCCCCCACGGCACCcgcggccc                                                                 >  2:8828917/1‑146 (MQ=255)
                                                                                           cATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATa                                                           <  2:8001590/150‑1 (MQ=255)
                                                                                              ggAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAgcg                                                        <  1:9043160/150‑1 (MQ=255)
                                                                                              ggAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAgcg                                                        >  2:2329169/1‑150 (MQ=255)
                                                                                                  tCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCCTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATc                                                    >  2:5664644/1‑150 (MQ=255)
                                                                                                     ctctctGTTGTCGATATTTTCTTTGACATGGGGGATTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTc                                                 <  1:3901658/150‑1 (MQ=255)
                                                                                                      tctctGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCg                                                <  2:4081614/150‑1 (MQ=255)
                                                                                                       ctctGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCgg                                               >  2:6591940/1‑150 (MQ=255)
                                                                                                          tgttgtCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCtt                                            <  2:8094867/150‑1 (MQ=255)
                                                                                                            ttgtCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTtct                                          >  2:4249027/1‑150 (MQ=255)
                                                                                                              gtCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCg                                        <  1:1018979/150‑1 (MQ=255)
                                                                                                               tCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGc                                       >  2:1366069/1‑150 (MQ=255)
                                                                                                               tCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGc                                       <  2:3687369/150‑1 (MQ=255)
                                                                                                               tCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCAGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCAATAACGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGc                                       >  1:7909553/1‑150 (MQ=255)
                                                                                                                  atatTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCtaa                                    >  2:8302778/1‑150 (MQ=255)
                                                                                                                   tatTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCtaat                                   >  1:64529/1‑150 (MQ=255)
                                                                                                                     ttttCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCtaataa                                 >  1:3221691/1‑150 (MQ=255)
                                                                                                                      tttCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATaaa                                >  1:8652009/1‑150 (MQ=255)
                                                                                                                         cTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGc                             <  1:9266622/150‑1 (MQ=255)
                                                                                                                         cTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGc                             <  1:8296699/150‑1 (MQ=255)
                                                                                                                          tttGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCa                            >  1:5764619/1‑150 (MQ=255)
                                                                                                                          tttGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCa                            >  1:1899674/1‑150 (MQ=255)
                                                                                                                          tttGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCa                            >  2:6207516/1‑150 (MQ=255)
                                                                                                                           ttGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCAt                           <  1:5222282/150‑1 (MQ=255)
                                                                                                                           ttGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCAt                           >  1:8520615/1‑150 (MQ=255)
                                                                                                                             gACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGc                                                                      >  1:2152551/1‑105 (MQ=255)
                                                                                                                              aCATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGc                        >  2:334304/1‑150 (MQ=255)
                                                                                                                                 tGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTa                     <  2:1138322/150‑1 (MQ=255)
                                                                                                                                  gggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTAc                    <  2:3120280/150‑1 (MQ=255)
                                                                                                                                   ggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACt                   >  1:284325/1‑150 (MQ=255)
                                                                                                                                   ggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACt                   >  1:5364189/1‑150 (MQ=255)
                                                                                                                                   ggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACt                   >  1:4498730/1‑150 (MQ=255)
                                                                                                                                   ggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACt                   >  2:215663/1‑150 (MQ=255)
                                                                                                                                    gggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACtt                  <  1:1040038/150‑1 (MQ=255)
                                                                                                                                    gggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACtt                  >  1:6877122/1‑150 (MQ=255)
                                                                                                                                       cTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGt               >  2:3181702/1‑150 (MQ=255)
                                                                                                                                        ttAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAgcgc                                                       <  2:681122/109‑1 (MQ=255)
                                                                                                                                        ttAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTc              <  1:7781041/150‑1 (MQ=255)
                                                                                                                                         tAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGTCAGCAGGCTCTTAAGCACCATTACCGCCCAGGTCAACATCGTCACCGGATAGCGCATCTTGGGCTTCTCGCTAATAAAAGCTTGGCGTACTTTGTcc             <  2:2260476/150‑1 (MQ=255)
                                                                                                                                            gcgcgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTccacc          >  2:7063387/1‑150 (MQ=255)
                                                                                                                                             cgcgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTccacca         >  1:5961183/1‑150 (MQ=255)
                                                                                                                                             cgcgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTccacca         <  1:4301645/150‑1 (MQ=255)
                                                                                                                                              gcgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCcaccac        >  1:3008069/1‑150 (MQ=255)
                                                                                                                                              gcgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCcaccac        >  2:1584323/1‑150 (MQ=255)
                                                                                                                                               cgcgcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCaccacc       <  2:6698281/150‑1 (MQ=255)
                                                                                                                                                  gcTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCt    >  2:4433284/1‑150 (MQ=255)
                                                                                                                                                    ttCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTc  >  2:4825611/1‑150 (MQ=255)
                                                                                                                                                    ttCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTc  >  2:2500036/1‑150 (MQ=255)
                                                                                                                                                    ttCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTc  >  2:6328283/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACCGCCCAGGTCACCAGCGTCACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCATGGCGTACTTTGTCCACCACCGCTTC  >  NC_000913/518186‑518483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: