Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,015 T→A 5.3% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0150TA 5.3% 1469.9 / 7.4 475intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base T (181/251);  new base A (37/3);  total (221/254)
Fisher's exact test for biased strand distribution p-value = 1.37e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 498 total aligned reads displayed.

ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164
                                                                                                                                                     |                                                                                                                                                     
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCAAGAAACAGCAAACAATCCAAAAAGCCGCGTTCAGCGGCGTTTTTTCTTCATTTATACGAGAATTAATTACTCATAGCAAt                                                                                                                                                       >  2:1021907/1‑150 (MQ=25)
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                       >  1:5336111/1‑150 (MQ=255)
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                       >  1:3148967/1‑150 (MQ=255)
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTTCTTTTATTCGCGAAATAAATACGCTTCGCAAtt                                                                                                                                                      >  2:39115/1‑150 (MQ=37)
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATACGCTTCGCAAtt                                                                                                                                                      >  2:6736341/1‑150 (MQ=255)
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGAGAAATAATTCAGCTTAGCAAtt                                                                                                                                                      >  1:8416566/1‑150 (MQ=255)
  gATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAAGCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAttt                                                                                                                                                     <  1:7659672/150‑1 (MQ=255)
     ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                  >  1:6807281/1‑150 (MQ=255)
     ctctCCAATATATTGAGTTTGCGTGCTTTTTTAGGCCGGATAAGGCGTTCACCCCCCATCCCCCAACAAACACCAAACAAACCAAAACGCCGCGTTAAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                  <  1:821693/150‑1 (MQ=37)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                             <  2:4443959/150‑1 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATTCTTTTGATCGCGAAATAAATACCCGTCGCCAATTATACaaaa                                                                                                                                             >  1:2740615/1‑150 (MQ=21)
           aaTATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaa                                                                                                                                            <  2:1464891/150‑1 (MQ=255)
           aaTATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaa                                                                                                                                            <  1:9227606/150‑1 (MQ=255)
              atatTGAGGTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaata                                                                                                                                         <  2:4853712/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                       <  1:6509140/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                       <  1:4803012/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                      <  2:8065168/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAAATCCGCTTCGCAAATTATCCaaaaaataaaa                                                                                                                                      >  2:5068406/1‑149 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTAGCGAAATAATTCCGCGTAGCAATTTATCCAAAaaataaat                                                                                                                                      >  2:8830253/1‑150 (MQ=38)
                   gAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTACGCTTAGCAATTTATACATAAAATAAAttt                                                                                                                                    >  1:2108075/1‑150 (MQ=255)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTAGCGAATTAATTACGCATCGCAAATTATCCAAAAAATAAAATTaa                                                                                                                                  >  1:4381723/1‑150 (MQ=38)
                      tttGCTGGCTGTGGTAGTCCGGATAAGGCTTTCACGCCGCATCCGGCTAGAAACAGCAAACAATCGAAAACGGCGCGTTCAGCTGCGTTTTTTCTGCTTTTCTTCGCGACGTTATTCAGCGTCGCAATTGTTCCATACAATAAATTTaaa                                                                                                                                 <  2:6089465/150‑1 (MQ=255)
                      tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAAATTaaa                                                                                                                                 >  2:8964332/1‑150 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  1:8792481/150‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  2:117098/150‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCTTAGCAATTTATACATAAAATAAATTTAAAATaa                                                                                                                             >  1:1924727/1‑150 (MQ=255)
                          cGTGCATTAGAAGGCCGGATAAGGCGTACACCCCCCAACCGCCAACAAACACCAAACAATCCAAAACGCCGCGTTCACCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  2:1812755/150‑1 (MQ=255)
                           gTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCCGCGGCGTGTTTTCTGCGGTTCTGCGCGAATTAATTCCGCGTCGCAAGTTATCCAAAAAAAAAAAGTAAAATAAc                                                                                                                            >  2:3427400/1‑150 (MQ=37)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACATCAAACAATCCAAATCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACa                                                                                                                           <  1:5580406/150‑1 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTTCTTTTATTAGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaaaa                                                                                                                           >  2:8648743/1‑148 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTCCGCTTCGCAATTTATACATAAAATAAATTTAAAATAACa                                                                                                                           >  1:5998654/1‑150 (MQ=255)
                              cTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTAGCAATTTATCCATAAAATAAATTTAAAATaaaaaa                                                                                                                         >  1:6165659/1‑150 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaaaa                                                                                                                        >  2:3106086/1‑150 (MQ=255)
                                   gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATa                                                                                                                    <  1:2542907/150‑1 (MQ=255)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                   <  2:8397217/150‑1 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                >  2:5637372/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTACGCTTCGCAATTTATACATAAAATAAAATTAAAATAAAAAAAaataaata                                                                                                                >  1:3687901/1‑150 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                              <  1:388581/150‑1 (MQ=255)
                                          ggATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTGTTCGCGAATTACTTGCGCGTGGCAATTTATCCCTAAAATAAATTTTAAATAAAAAAACATAATtaaat                                                                                                             >  1:4191803/1‑150 (MQ=255)
                                          ggATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTAGCGAATTAATTCCGCATCGCAAATTATACAAAAAAAAAATTTAAAATAAAAAAAAATAATtaaaa                                                                                                             >  1:5822324/1‑149 (MQ=255)
                                            aTAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaataa                                                                                                           <  2:8430174/150‑1 (MQ=255)
                                             tatggCGTTCACGCCGCATCCGTCAATAAACAGAAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaataaa                                                                                                          <  1:3357908/147‑1 (MQ=255)
                                             tAAGGCTTTCACGCCGCATCCGGAAAGAAACAGAAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaataaa                                                                                                          <  1:6333156/150‑1 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  1:4977699/150‑1 (MQ=255)
                                                ggCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTATTCGCGAATTAAATACGCGGCGCAAATTATACAAAAAATAAATTTAAAATAAAAAaaaaaaaataaaaaaaatt                                                                                                       >  2:4019812/1‑149 (MQ=255)
                                                      cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCg                                                                                                 <  2:4249604/150‑1 (MQ=255)
                                                      cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAAACg                                                                                                 >  2:8274013/1‑150 (MQ=255)
                                                              aTCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATc                                                                                         <  1:144119/150‑1 (MQ=255)
                                                               tCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCGTCGCGAATTAATTCCCCGTCGCAATTTATCCATAAAATAAAATTAAAATAACAAAACATAATTAAATAAAATGTTACCGCGTTCATCt                                                                                        >  2:1689180/1‑150 (MQ=255)
                                                                ccGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCtt                                                                                       <  2:459816/150‑1 (MQ=255)
                                                                 cGGCAAGAAACAGCAAACAATCCACAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCAACTTg                                                                                      <  1:4511560/150‑1 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                    <  1:1816961/150‑1 (MQ=255)
                                                                    cAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTg                                                                                   <  2:5558987/150‑1 (MQ=255)
                                                                         aaCAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGatgc                                                                              <  2:916435/150‑4 (MQ=255)
                                                                           cAGCAAACAATCCAAAACGCCGAGTTCAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTc                                                                            <  1:3801605/150‑1 (MQ=255)
                                                                            aGCAAACAATCCAACCCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATACTTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  2:5778132/150‑1 (MQ=255)
                                                                            aGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  1:7933477/150‑1 (MQ=255)
                                                                             gCAACCAATCCAACACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCAc                                                                          <  1:1089373/150‑1 (MQ=255)
                                                                              cAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAAAATAATTAAATAAAATGTAACCGCTTTCAACTTGCTGGAATTTCACg                                                                         >  2:2895448/1‑150 (MQ=255)
                                                                               aaaCAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATACATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATTTAACCGCTTTCATCTTGCTGGAATTTCACGc                                                                        >  2:2158374/1‑150 (MQ=255)
                                                                                 aCAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCtt                                                                      <  2:7325578/150‑1 (MQ=255)
                                                                                  cAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCttt                                                                     <  1:812111/150‑1 (MQ=255)
                                                                                      ccAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAtt                                                                 <  2:2647195/150‑1 (MQ=255)
                                                                                      ccAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAtt                                                                 <  2:6001517/150‑1 (MQ=255)
                                                                                       cAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttc                                                                <  2:3987672/150‑1 (MQ=255)
                                                                                        aaaaCGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTAGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAACATAATTAAATAAAATTTAACCGCTTTAATCTTTGTGGAATTTCACGCTttttttat                                                               >  2:5193105/1‑148 (MQ=255)
                                                                                         aaccgccgcGTACAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCCTCGCAATTTATCCATAAAATAAATTTAAAATAACCAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttctt                                                              <  2:2456226/147‑1 (MQ=255)
                                                                                         aaaCCCCGCCTACACCGGCGAATTATCTGCATTTCTTCGCGAATAAATACCGCTTCGCAATTTAACCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttctt                                                              <  1:4876017/150‑1 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGc                                                      <  2:6905764/150‑1 (MQ=255)
                                                                                                     cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCttt                                                  <  2:1339096/150‑1 (MQ=255)
                                                                                                        cggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTAATCTTTCTGGAATTTCAAGCTTTTATTCTTCTGCAAGACTTTCaa                                               >  2:5382391/1‑150 (MQ=255)
                                                                                                          gcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAAcc                                             <  2:6553577/150‑1 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          <  2:2334649/150‑1 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          >  1:5246706/1‑150 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          <  1:6737446/150‑1 (MQ=255)
                                                                                                             ttttttATGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTGATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGACTTTCAACCGCa                                          <  1:7109894/150‑1 (MQ=255)
                                                                                                               ttttcttcttttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCaaa                                        <  2:7183046/150‑1 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATATCAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  2:7057645/150‑1 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  2:1897003/150‑1 (MQ=255)
                                                                                                                     gCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAg                                  <  1:8613847/150‑1 (MQ=255)
                                                                                                                       ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                >  1:5144318/1‑150 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAATACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCGTTTCAACCGCAAACTTAAGCCTTGTa                           >  1:2445321/1‑150 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      <  2:2711909/150‑1 (MQ=255)
                                                                                                                                  aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                     <  2:4955501/150‑1 (MQ=255)
                                                                                                                                  aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                     <  2:8772191/150‑1 (MQ=255)
                                                                                                                                  aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                     >  2:3167346/1‑150 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   >  1:7193971/1‑150 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   >  2:3172592/1‑150 (MQ=255)
                                                                                                                                     taattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatc                  <  1:8895503/150‑1 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              >  1:1177413/1‑150 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              >  2:3547476/1‑150 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa            <  2:6282578/150‑1 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa            >  1:7478354/1‑150 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAACCATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTACAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaaa           <  1:2597409/150‑1 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCtaaa           >  2:7846621/1‑150 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaaa           <  2:3729793/150‑1 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaaa           <  1:2242232/150‑1 (MQ=255)
                                                                                                                                               taggtaTTTATCAATAAATTAAATTTAAAATATCATTACATAATTTTATAAAATGTAACCTCTTTCATCTTGCTGGAATTTCTCGCTTTTATTCTTCTGGAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        <  2:9314343/145‑1 (MQ=255)
                                                                                                                                                 gCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgt      >  1:4006706/1‑150 (MQ=255)
                                                                                                                                                   aaTTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCACACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGc    >  1:62667/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑