Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,227 A→C 5.5% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2270AC 5.5% 1363.0 / 12.3 434intergenic (‑188/+180)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base A (161/235);  new base C (29/2);  total (193/241)
Fisher's exact test for biased strand distribution p-value = 3.03e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

AATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351078‑1351376
                                                                                                                                                      |                                                                                                                                                     
   cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                                                                    <  1:5123591/150‑1 (MQ=255)
   cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                                                                    <  2:8127766/150‑1 (MQ=255)
    aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACACCCCACAgg                                                                                                                                                   >  2:8782600/1‑150 (MQ=255)
      gCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCTCACCCCAGAgggg                                                                                                                                                 >  2:3631750/1‑150 (MQ=255)
         aTTCGTCGTTTTAGTAAACAGTACGAACAGATACCCGGTTATTAGCATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                                                              <  2:7651990/150‑1 (MQ=39)
         aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                                                              <  1:8661620/150‑1 (MQ=255)
         aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                                                              <  2:7510450/150‑1 (MQ=255)
         aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCACACCCCAGAGGGGCGa                                                                                                                                              >  2:6434198/1‑150 (MQ=255)
          ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                             <  1:1263313/150‑1 (MQ=255)
          ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCCAACCCACACCCCACAGGGGCggg                                                                                                                                             >  2:4310878/1‑148 (MQ=255)
           tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATAAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGCACCCGAACCCTCTCCCCACAGGGGCGAgg                                                                                                                                            >  1:5384879/1‑150 (MQ=255)
            cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                                                           <  1:1105008/150‑1 (MQ=255)
            cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                                                           <  2:8554740/150‑1 (MQ=255)
            cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                                                           <  2:1453609/150‑1 (MQ=255)
                gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCAAACCCACACCCCCCAGGGGCGAGGGGGcc                                                                                                                                       >  1:1967920/1‑150 (MQ=255)
                 ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACACCCCACAGGGGCGAGGGGGCCg                                                                                                                                      >  1:1705821/1‑150 (MQ=255)
                 ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACCCCCGAACCCACTCCCCAGAGGGGCGAGGGGCCCg                                                                                                                                      >  2:8838542/1‑150 (MQ=255)
                   ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCACACCCAAACCCACTCCCCACAGGGGCGAGGGGGcccga                                                                                                                                    >  2:4742115/1‑147 (MQ=255)
                     aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                  <  1:3302527/150‑1 (MQ=255)
                     aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCTCGCCCCACAGGGGCGAGGGGACCGTAtt                                                                                                                                  >  1:631585/1‑150 (MQ=255)
                      gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGGTTCCCCTCACCCGAAACCACACCCCCGAGGGGCGAGGGGCCCGTATtg                                                                                                                                 >  1:1156337/1‑150 (MQ=255)
                        aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACACCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                               >  1:1561670/1‑150 (MQ=255)
                             gTACGAACAGATAAACGCTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCACCTCCGACCGCAGCAGGCAGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCGAATTGTCCAAat                                                                                                                          <  2:4882885/150‑1 (MQ=38)
                              tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGGCTTTGCCCCCCACCCAACCCCAAACCCCCGCGGGGCGGGGGGCCCGAATGTTGCaaaa                                                                                                                          >  1:9057276/1‑149 (MQ=255)
                                cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGTGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                       <  1:6715240/150‑1 (MQ=255)
                                cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                       >  1:4669410/1‑150 (MQ=255)
                                  aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCACACCCAAACCCACACCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTGt                                                                                                                     >  1:6118534/1‑150 (MQ=255)
                                   aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGGCCTCACCCTATACCGCTGCCCAGTGGGGGGGGGGGGGCGTTTTTTGGAAATATtttt                                                                                                                    >  2:2506813/1‑150 (MQ=255)
                                       aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                                                                                >  2:5148173/1‑150 (MQ=255)
                                           aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGc                                                                                                            <  1:5664093/150‑1 (MQ=255)
                                             ggTTATTATAATCAACCTGGCTGTGAGTAGCTATATTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATGGTGCAAATATTGTTACCCCAGcaa                                                                                                          >  2:8452188/1‑150 (MQ=255)
                                             ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa                                                                                                          >  2:1266914/1‑150 (MQ=255)
                                                 attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                      <  2:4537212/150‑1 (MQ=255)
                                                   tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                    <  1:2592252/150‑1 (MQ=255)
                                                    ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                                   <  1:7385797/150‑1 (MQ=255)
                                                       aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACCCCCTAACCCAAGCCCCCGAGGGGCGACGGGACCCTATTGTTCAAAAATTGTTACCCCCCCACCACACAGGcg                                                                                                >  1:4873641/1‑149 (MQ=255)
                                                        tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGCACCCCAACCCACACCCCCGCGGGGCGAGGGGGCCGGTTTGTGGAAAAATTTTTTACCCAGCAACAAACAGGcgc                                                                                               >  1:4360895/1‑148 (MQ=255)
                                                        tCAACCTGGCTGTGAGTAGCTATAGTGGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                               <  2:2226544/150‑1 (MQ=255)
                                                               ggCTGTGAGTAGCTATATTTGCCAGGTCCGACCGGAGCAGGCTGCGGGAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGTGGGGAGTGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  2:9300520/150‑1 (MQ=39)
                                                                  tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTTATACAGcccc                                                                                     <  1:8115107/150‑1 (MQ=255)
                                                                  tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcccc                                                                                     <  1:3910407/150‑1 (MQ=255)
                                                                  tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCACACCCAAAACCACACCCCACAGGGGCGAGGGGGCCGGATTGTTCAAAAAATTTTTAACCAGCAACAAAAAGGCGCAAAAAGcccc                                                                                     >  1:5894829/1‑150 (MQ=255)
                                                                     gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGCGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  2:1560312/150‑1 (MQ=255)
                                                                       gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                <  1:4495123/150‑1 (MQ=255)
                                                                         aGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCACACCCGAAACCACACCCCACAGGGGCGGGGGGGCCCGGTTTTGGAAATATTTTTTAACCCCCAACAAAAACGCTCATACACCCCCAAAcccc                                                                              >  1:5461838/1‑149 (MQ=255)
                                                                         aGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt                                                                              <  1:2619896/150‑1 (MQ=255)
                                                                         aGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTCACCCt                                                                              <  2:3185108/150‑1 (MQ=255)
                                                                          gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCtt                                                                             <  2:7828456/150‑1 (MQ=255)
                                                                           cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGCACCCCAACCCACTCCCCCGAGGGGCGGGGGGGCCGGGTTTTGGGAATATTTTTGAACCAGCCCCAAAAACCCGCAAACAACCCCAAAccccgt                                                                            >  1:3414861/1‑147 (MQ=255)
                                                                           cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTAACCTCACCCTAAACCTCTACCCAGTGGGGGGTGGGGGACGTATTGTTGAAATATTTTTTACCCAGGAACAAAAATGGTGATACAGCCCCGAAACCgtt                                                                            >  2:3231146/1‑150 (MQ=255)
                                                                            tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                           <  2:6813064/150‑1 (MQ=255)
                                                                            tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                           <  1:1390331/150‑1 (MQ=255)
                                                                            tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCAAACCCGAACCCACTACCCAGAGGGGCGCGGGGACCCTATTTTGCAAATATTTTTAACCCACCAACAAACAGGCTCCTAAACCCCCGAACCCctta                                                                           >  2:8002819/1‑149 (MQ=255)
                                                                                 ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCGCACCCGAACCCACGCCCCAGAGGGGCGCGGGGGCCCGATTGTGGAAATATTTTTTACCCACCAACAAACACGCGCAAAAAGCCCCGAACCCGGTCCTGGc                                                                      >  1:8257292/1‑150 (MQ=255)
                                                                                    ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGAGGGGCCCGTATTGTGCAAATATTGTTACCCCAGCCACAAACCGGCGCAAACAGCCCCTCACCCGATCATGGCGag                                                                   >  2:8264791/1‑149 (MQ=38)
                                                                                         tCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTg                                                              <  1:7826552/150‑1 (MQ=255)
                                                                                          ccGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTgg                                                             <  1:3596577/150‑1 (MQ=255)
                                                                                          ccGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTgg                                                             <  2:9040977/150‑1 (MQ=255)
                                                                                            gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                           <  1:2813601/150‑1 (MQ=255)
                                                                                            gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                           <  2:6283401/150‑1 (MQ=255)
                                                                                            gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                           <  2:2113107/150‑1 (MQ=255)
                                                                                              ccGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACg                                                         <  2:4087885/150‑1 (MQ=255)
                                                                                               cGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACgg                                                        <  1:6594647/150‑1 (MQ=255)
                                                                                               cGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACgg                                                        <  2:6543815/150‑1 (MQ=255)
                                                                                               cGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACgg                                                        <  1:7542777/150‑1 (MQ=255)
                                                                                                ggAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGt                                                       <  2:5248384/150‑1 (MQ=255)
                                                                                                  aGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTc                                                     <  1:9254095/150‑1 (MQ=255)
                                                                                                   gCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCa                                                    <  1:8423003/150‑1 (MQ=255)
                                                                                                      ggCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                 <  2:2669866/150‑1 (MQ=255)
                                                                                                       gCTGCGGCAGGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCACCCCAGCGGGGCGAGGGGACCGGATTGTGCAAATAATGTTACCCCAGCCACAAACAGGCTCATACAGCCCCAAACCCTTTCAAGGCGCTGGCTGGGACGGTTCAGAcc                                                >  2:8948246/1‑150 (MQ=255)
                                                                                                        cTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCACCCCAGAGGGGCGAGGGGCCCGTATTGTGCAAATATTGTTACCCCCGCCACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCt                                               >  2:9214415/1‑150 (MQ=255)
                                                                                                           cGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                            <  1:3175616/150‑1 (MQ=255)
                                                                                                           cGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAACCCAGCAACAAACAGGCTCATACAGCCCCGAACCCGTTAATGGCGATGGCTGGGACGGTTCAGACCGTGc                                            >  2:2882843/1‑150 (MQ=38)
                                                                                                            ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                           <  1:4083526/150‑1 (MQ=255)
                                                                                                            ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                           <  1:2153051/150‑1 (MQ=255)
                                                                                                                    ggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATAtt                                    <  1:3071670/150‑1 (MQ=255)
                                                                                                                     gggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtc                                   <  1:7575863/150‑1 (MQ=255)
                                                                                                                       gcgcTTTTCCCCTCACCCTACCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                 <  2:6679172/150‑1 (MQ=255)
                                                                                                                       gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                 <  1:1848713/150‑1 (MQ=255)
                                                                                                                           ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                             <  2:5711988/150‑1 (MQ=255)
                                                                                                                            tttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCa                            <  1:3715451/150‑1 (MQ=255)
                                                                                                                             ttCCCCTCACCCTAACCCGCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCACATATTGTTACCCCAGCAACCAACAGGCTCATACAGCCCCGAACCCGTTCATGGCGATGGCTGGGACGGGGTAGACCGTGCCGAAGATTCTCCCGccc                           >  2:1197726/1‑148 (MQ=37)
                                                                                                                             ttCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCGTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAc                           >  2:3488296/1‑150 (MQ=255)
                                                                                                                              gccccTCACCCCAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAcc                          <  2:3855022/149‑1 (MQ=255)
                                                                                                                                cccTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGt                        >  1:8869204/1‑150 (MQ=255)
                                                                                                                                   tCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCGGGGACGGTTCAGACCTTGCCGAATATTCTCCCGCACCGgctc                     >  1:2908400/1‑150 (MQ=255)
                                                                                                                                   tCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTCTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtctc                     <  2:6876045/150‑1 (MQ=255)
                                                                                                                                        cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                >  1:5758681/1‑150 (MQ=255)
                                                                                                                                         tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCttt                                                                            <  1:1004261/89‑1 (MQ=255)
                                                                                                                                         tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCttt                                                                            >  2:1004261/1‑89 (MQ=255)
                                                                                                                                         tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt               >  1:3001572/1‑150 (MQ=255)
                                                                                                                                         tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt               <  2:7170971/150‑1 (MQ=255)
                                                                                                                                          tccccTCGCCCCTCTGGGGAGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGttt              >  1:1807554/3‑150 (MQ=255)
                                                                                                                                             ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           <  2:5384879/150‑1 (MQ=255)
                                                                                                                                              ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          <  2:6096963/150‑1 (MQ=255)
                                                                                                                                               tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  2:4622155/1‑150 (MQ=255)
                                                                                                                                                ctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacca        >  1:2501791/1‑150 (MQ=255)
                                                                                                                                                   cccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaa     >  2:3005319/1‑150 (MQ=255)
                                                                                                                                                   cccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTCTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaa     <  1:7122983/150‑1 (MQ=255)
                                                                                                                                                      agagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAg  <  2:1941510/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351078‑1351376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: