Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,008,698 T→G 5.1% I141S (ATT→AGT)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,008,6980TG 5.1% 1410.6 / 7.5 514I141S (ATT→AGT) yedEUPF0394 family sulphur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (232/244);  new base G (2/36);  total (234/280)
Fisher's exact test for biased strand distribution p-value = 2.43e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 525 total aligned reads displayed.

CTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGG  >  NC_000913/2008549‑2008847
                                                                                                                                                     |                                                                                                                                                     
 tttGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAtt                                                                                                                                                      >  2:5833276/1‑150 (MQ=255)
      cGCAGCGCTGTGGGCCAACAATGTCAAACTGCTAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGTCGGCGTTCTTTACCGGTATTCCTCa                                                                                                                                                 <  1:6762688/150‑1 (MQ=255)
      cGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCa                                                                                                                                                 >  1:5028849/1‑150 (MQ=255)
      cGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCa                                                                                                                                                 <  2:530347/150‑1 (MQ=255)
      cGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCa                                                                                                                                                 <  1:931713/150‑1 (MQ=255)
        cAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGt                                                                                                                                               <  1:6431060/150‑1 (MQ=255)
        cAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGt                                                                                                                                               >  1:6583728/1‑150 (MQ=255)
            gcTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTtctc                                                                                                                                           <  1:8847465/150‑1 (MQ=255)
                  ggCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCa                                                                                                                                     >  2:199838/1‑150 (MQ=255)
                  ggCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCa                                                                                                                                     >  2:8085452/1‑150 (MQ=255)
                  ggCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCAGTATGCAGGCCATCATTGGCGGCATTATCGCAGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACAGGTATTCCACAGTTCTCgcagca                                                                                                                                     >  2:4198973/1‑150 (MQ=255)
                     caacaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGc                                                                                                                                  <  1:1288653/150‑1 (MQ=255)
                      aacaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCCGTTCTCGCTGCATGcc                                                                                                                                 <  2:1911026/150‑1 (MQ=255)
                      aacaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGcc                                                                                                                                 <  2:6318425/150‑1 (MQ=255)
                       acaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCt                                                                                                                                <  2:2694929/150‑1 (MQ=255)
                       acaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCt                                                                                                                                <  2:3993662/150‑1 (MQ=255)
                       acaaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCt                                                                                                                                <  1:7246274/150‑1 (MQ=255)
                        caaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTg                                                                                                                               >  2:6389755/1‑150 (MQ=255)
                         aaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTgg                                                                                                                              >  2:1087593/1‑150 (MQ=255)
                         aaTGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTgg                                                                                                                              <  1:752560/150‑1 (MQ=255)
                               aaaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCttt                                                                                                                        <  1:8748078/150‑1 (MQ=255)
                               aaaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCttt                                                                                                                        <  1:7804069/150‑1 (MQ=255)
                               aaaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTAGCTGCATGCCTGGTTCttt                                                                                                                        >  1:11458/1‑150 (MQ=255)
                               aaaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCttt                                                                                                                        <  2:7767040/150‑1 (MQ=255)
                               aaaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGGGTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCGTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCttt                                                                                                                        >  1:4664827/1‑150 (MQ=255)
                                aaCTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTg                                                                                                                       <  2:7349436/150‑1 (MQ=255)
                                aaCTGCGAATGCCGCGCAGCCGTATCCGCATGATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTg                                                                                                                       <  1:6290109/150‑1 (MQ=255)
                                  cTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGcc                                                                                                                     <  1:7075225/150‑1 (MQ=255)
                                  cTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGcc                                                                                                                     <  1:3285853/150‑1 (MQ=255)
                                         tGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCa                                                                                                              >  2:905082/1‑150 (MQ=255)
                                          gCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCAc                                                                                                             >  1:8542063/1‑150 (MQ=255)
                                           ccGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCGTGCCTGGTTCTTTGCCATCGCCACt                                                                                                            >  2:4397928/1‑150 (MQ=255)
                                            cgcgCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTg                                                                                                           >  2:7943159/1‑150 (MQ=255)
                                              cgcAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGcc                                                                                                         <  1:4077560/150‑1 (MQ=255)
                                                cAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCAt                                                                                                       <  1:2380354/150‑1 (MQ=255)
                                                 aGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCAtt                                                                                                      <  2:3836173/150‑1 (MQ=255)
                                                 aGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCAtt                                                                                                      >  1:5383451/1‑150 (MQ=255)
                                                   ccGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTgg                                                                                                    >  2:6799607/1‑150 (MQ=255)
                                                   ccGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTgg                                                                                                    >  2:3113363/1‑150 (MQ=255)
                                                      tATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTc                                                                                                 >  2:2503049/1‑150 (MQ=255)
                                                      tATCCGCATTATGCAGGCCATCATCGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTCTTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCAGTGGTTc                                                                                                 >  2:4624741/1‑150 (MQ=255)
                                                       aTCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCa                                                                                                <  1:810319/150‑1 (MQ=255)
                                                        tCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCAt                                                                                               <  1:8273488/150‑1 (MQ=255)
                                                        tCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCAt                                                                                               >  2:5174985/1‑150 (MQ=255)
                                                         ccGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGCTCATg                                                                                              >  1:896331/1‑150 (MQ=255)
                                                           gCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGt                                                                                            >  2:5980842/1‑150 (MQ=255)
                                                            cATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGtt                                                                                           <  1:4782375/150‑1 (MQ=255)
                                                               tATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTgg                                                                                        <  2:8695156/150‑1 (MQ=255)
                                                                 tGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGgcg                                                                                      >  1:7880001/1‑150 (MQ=255)
                                                                  gCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGgcgc                                                                                     <  1:6970210/150‑1 (MQ=255)
                                                                    aGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGgcgcgc                                                                                   >  1:8486653/1‑150 (MQ=255)
                                                                      gCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGgcgcgcgc                                                                                 >  2:7594023/1‑150 (MQ=255)
                                                                       ccATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCt                                                                                <  1:2739637/150‑1 (MQ=255)
                                                                          tcatTGGCGGCATTATCGCGGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACGGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGACACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTa                                                                             <  1:5952558/150‑1 (MQ=255)
                                                                           catTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTAc                                                                            <  2:2195186/150‑1 (MQ=255)
                                                                               ggcggcATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCctg                                                                        <  2:9210809/150‑1 (MQ=255)
                                                                                 cggcATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCctgct                                                                      >  2:3520309/1‑150 (MQ=255)
                                                                                  ggcATTATCGCCGGTTTTGGCGCGCGTCAGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTACTCAGTTCTCGCTGCAAGCCTGGTTATTTGCCAACGCCACTGCCAAAGGTTCATGGTTTTGCGCGCGCTTTACCctgctg                                                                     >  1:8824688/1‑150 (MQ=255)
                                                                                   gcATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCtgctgc                                                                    <  1:2966754/150‑1 (MQ=255)
                                                                                     aTTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGccc                                                                  <  2:5446995/150‑1 (MQ=255)
                                                                                         tCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCt                                                              <  1:9173955/150‑1 (MQ=255)
                                                                                           gCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTc                                                            >  2:2880066/1‑150 (MQ=255)
                                                                                           gCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTc                                                            <  1:567808/150‑1 (MQ=255)
                                                                                           gCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTc                                                            >  1:1752922/1‑150 (MQ=255)
                                                                                            ccGGTTTTGGCGCTCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCTCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTcc                                                           <  2:1389479/150‑1 (MQ=255)
                                                                                            ccGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTcc                                                           <  2:364896/150‑1 (MQ=255)
                                                                                               gTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTa                                                        >  2:2417987/1‑150 (MQ=255)
                                                                                                ttttGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTAt                                                       >  2:9106178/1‑150 (MQ=255)
                                                                                                     gcgcgcGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCg                                                  >  1:488115/1‑150 (MQ=255)
                                                                                                      cgcgcgTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGt                                                 <  1:3139951/150‑1 (MQ=255)
                                                                                                      cgcgcgTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGt                                                 <  1:5166811/150‑1 (MQ=255)
                                                                                                       gcgcgTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTg                                                >  2:8932315/1‑150 (MQ=255)
                                                                                                              tGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGGAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGc                                         <  1:3988148/150‑1 (MQ=255)
                                                                                                              tGGCAATGGGCTGTAACCGGGCGGCGTTCTGTACCGGTATTCCTCAGTTCTCGCTGCCTGCCTGGGTCTTTGCCATCGCCACTGCCATTGGTTCAGGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGc                                         >  2:5880359/1‑150 (MQ=255)
                                                                                                               ggCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCa                                        <  1:5508867/150‑1 (MQ=255)
                                                                                                                gCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAg                                       >  2:4986037/1‑150 (MQ=255)
                                                                                                                gCAATGGGCTGTAACCTGGCGGCGTTCTTTAACGGTATTACTCAGTTCTCGCTGCATGCCTGGGTCTTTGCCATCGCCACAGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCCTGAAAATTCAg                                       >  1:792369/1‑150 (MQ=255)
                                                                                                                   aTGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGaaa                                    <  2:610944/150‑1 (MQ=255)
                                                                                                                    tGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAg                                   >  2:7200609/1‑150 (MQ=255)
                                                                                                                        cTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGGTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTc                               <  2:7079267/150‑1 (MQ=255)
                                                                                                                         tGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTGTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCt                              <  2:5602933/150‑1 (MQ=255)
                                                                                                                           tAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTgc                            >  2:158826/1‑150 (MQ=255)
                                                                                                                              ccTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTgccgc                         >  2:8615369/1‑150 (MQ=255)
                                                                                                                              ccTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTgccgc                         <  2:7395807/150‑1 (MQ=255)
                                                                                                                               cTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTgccgcc                        <  1:2496799/150‑1 (MQ=255)
                                                                                                                               cTGGCGGCGTTCTTTACCGGGAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTgccgcc                        <  1:2048648/150‑1 (MQ=255)
                                                                                                                                tGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCt                       >  2:1754888/1‑150 (MQ=255)
                                                                                                                                tGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCt                       <  1:6237428/150‑1 (MQ=255)
                                                                                                                                tGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCt                       <  1:4598015/150‑1 (MQ=255)
                                                                                                                                     gcgTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCg                  <  1:4217999/150‑1 (MQ=255)
                                                                                                                                      cgTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGc                 >  2:1257930/1‑150 (MQ=255)
                                                                                                                                       gTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCt                <  1:2872757/150‑1 (MQ=255)
                                                                                                                                       gTTCTTTACCGGTAGTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCt                <  1:1590995/150‑1 (MQ=255)
                                                                                                                                        ttCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTg               >  2:8848995/1‑150 (MQ=255)
                                                                                                                                         tCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGa              <  2:149380/150‑1 (MQ=255)
                                                                                                                                           tttACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACg            >  1:6531395/1‑150 (MQ=255)
                                                                                                                                           tttACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACg            <  2:6490595/150‑1 (MQ=255)
                                                                                                                                                aggTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCaaaa       >  2:327748/2‑150 (MQ=255)
                                                                                                                                                   tATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAAcc    <  1:6126673/150‑1 (MQ=255)
                                                                                                                                                     ttCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCgg  <  1:3649457/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTGCCGCAGCGCTGTGGGCCAACAATGTCAAACTGCGAATGCCGCGCAGCCGTATCCGCATTATGCAGGCCATCATTGGCGGCATTATCGCCGGTTTTGGCGCGCGTCTGGCAATGGGCTGTAACCTGGCGGCGTTCTTTACCGGTATTCCTCAGTTCTCGCTGCATGCCTGGTTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGG  >  NC_000913/2008549‑2008847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: