Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,229 G→T 6.5% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2290GT 6.5% 1281.8 / 13.5 418intergenic (‑25/+283)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (201/170);  new base T (2/39);  total (207/211)
Fisher's exact test for biased strand distribution p-value = 1.53e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177080‑2177378
                                                                                                                                                     |                                                                                                                                                      
 gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                       <  1:1918249/150‑1 (MQ=255)
  cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAt                                                                                                                                                      >  1:5064513/1‑150 (MQ=255)
  cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAt                                                                                                                                                      <  1:4156509/150‑1 (MQ=255)
         gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                               <  2:2167973/150‑1 (MQ=255)
         gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                               >  2:1979777/1‑150 (MQ=255)
         gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                               <  2:139358/150‑1 (MQ=255)
          cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAACGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCAGTCGTTTGTTTTCGATTTAAAAat                                                                                                                                              >  1:1565049/1‑150 (MQ=255)
          cAGCGGTTTCTACCACCACATGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCCCAAAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAATTTAATATCCTGTCGTTTTTTTTAGATTTAAAAat                                                                                                                                              >  1:4359391/1‑150 (MQ=255)
           aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                             >  1:528087/1‑150 (MQ=255)
             cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                           <  2:3251510/150‑1 (MQ=255)
             cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCGTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                           >  1:2339173/1‑150 (MQ=255)
                  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCAATTTCAAAATATAATGaa                                                                                                                                      >  2:353841/1‑150 (MQ=255)
                       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                 <  1:8853296/150‑1 (MQ=255)
                       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAATGAAattat                                                                                                                                 >  1:4900311/1‑150 (MQ=255)
                       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCATGTCGTTTGTTTTAGATTTCAAAATATAATTAAattat                                                                                                                                 >  2:9332517/1‑150 (MQ=255)
                        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGACCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGATTTCAAAATATAAAGAAaatatt                                                                                                                                >  2:8176747/1‑150 (MQ=255)
                        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                <  1:1016633/150‑1 (MQ=255)
                        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                <  1:7925280/150‑1 (MQ=255)
                         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               <  1:3414418/150‑1 (MQ=255)
                         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               <  2:4708265/150‑1 (MQ=255)
                         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               >  2:5120358/1‑150 (MQ=255)
                         ccacGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               <  1:7538529/150‑1 (MQ=255)
                          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAtttt                                                                                                                              >  2:1684012/1‑149 (MQ=255)
                          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTAtttt                                                                                                                              >  1:7769086/1‑149 (MQ=255)
                           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                             <  1:2143802/150‑1 (MQ=255)
                            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCCGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                            <  1:6147667/150‑1 (MQ=255)
                             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAAATATTTGttt                                                                                                                           >  1:2922881/1‑150 (MQ=255)
                                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                        <  2:2462464/150‑1 (MQ=255)
                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCAATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                       >  2:4522797/1‑150 (MQ=255)
                                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGTAATTATTTTTTTTTtaa                                                                                                                      >  2:1812712/1‑150 (MQ=255)
                                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAata                                                                                                                    >  1:934399/1‑150 (MQ=255)
                                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCga                                                                                                                >  1:3943002/1‑150 (MQ=255)
                                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                <  2:7024084/150‑1 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                               <  2:6342772/150‑1 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                               >  2:1522830/1‑150 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                               <  2:5917093/150‑1 (MQ=255)
                                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                           <  1:634901/150‑1 (MQ=255)
                                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                           <  1:5357580/150‑1 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                       >  2:5470347/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                       <  2:7902590/150‑1 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTTATATCGGGGTAACGATc                                                                                                      >  1:405955/1‑150 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                      <  1:9198101/150‑1 (MQ=255)
                                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                     <  1:6883237/150‑1 (MQ=255)
                                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTCCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                     >  2:7492606/1‑150 (MQ=255)
                                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATcac                                                                                                    >  2:3571473/1‑150 (MQ=255)
                                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAACGATcac                                                                                                    >  2:8438571/1‑150 (MQ=255)
                                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                    >  2:2948692/1‑150 (MQ=255)
                                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTTAAATATTTTTTTTTAAATATAGAGATAAAGATCACaa                                                                                                  >  1:5578853/1‑150 (MQ=255)
                                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTAAAAATATAATGAAAATATTTGTTTTTTAATATCGAGATAACGATCACaa                                                                                                  >  1:5641206/1‑150 (MQ=255)
                                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAATTCAAAATAAAAAGAAAATATTTGTTTTTAAATATCGAGAAAACCATCACaa                                                                                                  >  2:8812664/1‑150 (MQ=255)
                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               >  1:14512/1‑118 (MQ=255)
                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                               >  1:5760081/1‑150 (MQ=255)
                                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAc                                                                                              >  2:6280396/1‑150 (MQ=255)
                                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGGTTTAAAAATATAATGAAATTATTTTTTTTTTAATATAGAGAGAAAGATAAAAAAAAAGACa                                                                                          >  1:3313883/1‑150 (MQ=255)
                                                                gAACCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAat                                                                                        <  2:7597641/150‑1 (MQ=255)
                                                                gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAat                                                                                        >  2:4148204/1‑150 (MQ=255)
                                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAata                                                                                       >  2:7798292/1‑150 (MQ=255)
                                                                    cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAATTAAATTATTTGTTTTTTAATATAGATATAAAAATAACAAAAACGAAAaaatta                                                                                    >  1:1733601/1‑148 (MQ=255)
                                                                     gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATAACGATAAAAAAAAAGAAAAAATGaa                                                                                   >  2:8060080/1‑150 (MQ=255)
                                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAat                                                                                >  2:6908445/1‑150 (MQ=255)
                                                                         aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                               >  2:7968096/1‑150 (MQ=255)
                                                                            cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                            <  2:7359267/150‑1 (MQ=255)
                                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTGAAAATATAAAGTAAATAATTTTTTTTTAATATAGAGATAAAGAACACAAAAACGACAAAAATTAAattatta                                                                           >  1:6407484/1‑149 (MQ=255)
                                                                                gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                        <  2:5018906/150‑1 (MQ=255)
                                                                                  ggtgtgCGTTGTCCCGCATCTGCTTTGTCTAAACAACGTACATTTTCATATCCTGTGTTTTGTTTTCTTTTTCAAAATATAATTAATTTTTTTTTTTTTAAATATCGACATAACGACCACAAAAACGACAATATGAAAATTATTCgagga                                                                      <  1:4773724/148‑1 (MQ=255)
                                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAGCGACAATATGAAAATTATTCGAGGAGTGaaa                                                                <  2:2564891/150‑1 (MQ=255)
                                                                                         gtttttAAGCATCTGCTTTGTCTATACCACTTAAATTTTCATATCCTGTGTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTGTTTTTAAATATCGATATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                               <  1:9105245/150‑1 (MQ=255)
                                                                                              tCAGCATCGGATTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                         <  2:9024793/150‑1 (MQ=255)
                                                                                               cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCAAAAAAACCACAATATGAAAAATATTAGAGGAGTGAAAGGCaaaa                                                        >  2:2703319/1‑150 (MQ=255)
                                                                                                   aTCTGTTTTTTCGATACCACTTAATTTTTAATATCCTGTGTTTTGTTTTGTATTTAAAAATTTATTTAATTTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  1:4449215/150‑1 (MQ=255)
                                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  2:4900311/150‑1 (MQ=255)
                                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  2:4359391/150‑1 (MQ=255)
                                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    >  2:6816844/1‑150 (MQ=255)
                                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATGAAATTATATTTTTTTAAATATCGAGAAAACGAAAACAAAAAACACAAAAATAAAAATATTAGAGGAGGGTAAGGCaaaaaaaag                                                   >  2:8585030/1‑148 (MQ=255)
                                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                   <  2:5645362/150‑1 (MQ=255)
                                                                                                     cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATTTTTAAAAAAAAAAAAAAAATATTTTTTTTTTAATAAATAGATAAACAACACAAAAAACACAAAAATAAAAATATTATAGGGGTGTAAGGCaaaaaaaaag                                                  >  1:4524633/1‑147 (MQ=255)
                                                                                                      tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGAATTAAAAAAAAAATTAAAATATTTTTTTTTTAATATAGTGCTAAACATCACAAAAACCACAAAATGAAAAATAATAGCGGCGTGTGAGGCACAAAAAACgc                                                  >  1:3723712/1‑149 (MQ=255)
                                                                                                       gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc                                                >  2:3837187/1‑150 (MQ=255)
                                                                                                             tctaaaCCACGTACATTTTAATATCTTTTCGTTTTTTTTCTTTTTAAAATTATATTTAATTTTTTTTTTTTAAAATATCGATATAACTACCAAAAAAACGAAAATATGAAAATTATTCTAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                          <  1:1220814/145‑1 (MQ=255)
                                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGCTa                                        >  2:7656997/1‑150 (MQ=255)
                                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                        <  2:3675236/150‑1 (MQ=255)
                                                                                                                aaaCCACTTAATTTTTAATATCCTTTGTTTTTTTTTCTTTTTAAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACTATCAAAAAAACTACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                       <  1:8113463/148‑1 (MQ=255)
                                                                                                                aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAg                                       >  2:6190785/1‑150 (MQ=255)
                                                                                                                 tACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGTTTTGAAAATATAATGGAAATATTTTTTTTTTAATATAGAGATAACGATCACAAAAACCACAAAATGAAAAATATTCGAGGGGTGTAAGGCAAAAAAAAGGCCACCCCATAgg                                      >  2:3331119/1‑150 (MQ=255)
                                                                                                                    cACTTAAATTTTAATATCCTGTCGTTTTTTTTCTATTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                   <  1:3653842/150‑1 (MQ=255)
                                                                                                                       gTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                <  2:6072528/150‑1 (MQ=255)
                                                                                                                        aataTTTTCTTATCCTGTTGTTTTTTTTCCTTTAAAAAATATAATTAATTTTTTTTTTTTTAATTATCTAGAAAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                               <  1:7178642/147‑1 (MQ=255)
                                                                                                                               taatatCCTTTCTTTTTTTTTCTATTAAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATAAAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                        <  1:2483408/148‑1 (MQ=255)
                                                                                                                               tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                        <  2:230779/150‑1 (MQ=255)
                                                                                                                                   atCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAATATGAAAAATAATCGAGGAGTGAAAGGCAAAAAAAAGGCCAACCGATAGGGAAGCCCTAGCAAAGTgc                    >  2:6550477/1‑150 (MQ=255)
                                                                                                                                       tgccGTTTGTTTTCTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                <  1:6808232/147‑1 (MQ=255)
                                                                                                                                       tGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                <  1:6030673/150‑1 (MQ=255)
                                                                                                                                         tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt              >  2:3113367/1‑150 (MQ=255)
                                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt            <  1:1315648/150‑1 (MQ=255)
                                                                                                                                             ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt          <  1:2643673/150‑1 (MQ=255)
                                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg       >  2:3724251/1‑150 (MQ=255)
                                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc      <  2:1284032/150‑1 (MQ=255)
                                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc      >  1:3871005/1‑150 (MQ=255)
                                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc      <  2:9240888/150‑1 (MQ=255)
                                                                                                                                                     gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa  <  1:701679/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177080‑2177378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: