Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 12.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT12.4% 1044.8 / 23.1 431intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (167/187);  new base T (34/34);  total (205/226)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394
                                                                                                                                                      |                                                                                                                                                      
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                                        >  1:6143156/1‑150 (MQ=255)
   cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGaaa                                                                                                                                                     >  1:5921738/1‑150 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                  >  2:717656/1‑150 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                  >  1:736513/1‑150 (MQ=255)
       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCATGTCGTTTGTTTTAGATTTCAAAATATAATTAAattat                                                                                                                                                 >  2:9332517/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  1:1016633/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTGAAATTATAATGTTAttttttt                                                                                                                                              >  2:7990250/1‑149 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATAttttt                                                                                                                                             >  1:5757233/1‑148 (MQ=255)
              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                          <  1:2651881/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATtttttttt                                                                                                                                         >  1:4852070/1‑150 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                        <  2:2462471/150‑1 (MQ=255)
                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTaaa                                                                                                                                      >  1:7779564/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTTAata                                                                                                                                    >  1:4267397/1‑150 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:6396617/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  1:1558633/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  1:3980728/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:822417/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                <  2:5103725/150‑1 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                              <  2:501853/150‑1 (MQ=255)
                           gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTTGTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATagagtt                                                                                                                             >  1:1221660/1‑148 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                           <  1:5357573/150‑1 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                           <  2:2281628/150‑1 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                          <  1:3903181/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                       <  1:5016342/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                       <  2:812551/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                       >  2:7672486/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                       <  2:7902633/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATAGAGATAACGAta                                                                                                                      >  1:405962/1‑149 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAGATATCGAGATAACGATc                                                                                                                      <  2:331872/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                      <  1:9198079/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATcac                                                                                                                    >  2:2730743/1‑150 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTGAAAATATTATGAAATTATTTGTTTTTTAATATGGAGTTAACGGTcac                                                                                                                    >  1:5580707/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                               >  1:14512/1‑118 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                               <  1:3170454/150‑1 (MQ=255)
                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAATAATTAAATTATTTTTTTTTTAATATAGAGATAAAGATCAaaaaaaag                                                                                                             >  1:1797636/1‑148 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                          >  2:4712881/1‑150 (MQ=255)
                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAata                                                                                                       >  1:8927167/1‑150 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                  >  2:2204094/1‑150 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATAAGaaa                                                                                                  >  2:2989960/1‑150 (MQ=255)
                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATGAAATTATTTTTTTTTTAATAAAGAGATAACGAGAACAAAAAAGACAATATGAAAat                                                                                                >  1:436284/1‑150 (MQ=255)
                                                            cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                            <  2:7359267/150‑1 (MQ=255)
                                                               cgcgcgGCGCGTGGTTCAGCATCTGCTTTGCCGAACCCACGTACATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAACTATCATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                         <  2:5577243/150‑1 (MQ=255)
                                                                  gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                      <  2:2605193/150‑1 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAATAATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTAgaggag                                                                                     >  2:3598768/1‑150 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACTTAAATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                    <  2:1873470/150‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCAAAAAAACGACAAAATTAAAATTATTCGAGGAGt                                                                                    >  2:1301887/1‑150 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAATAAATTATTTGTTTTTAAAAATAGAGATAACGATAAAAAAAACGAAAAAATTAAAAATATTAGAGGAGAGaaa                                                                                >  2:2358257/1‑150 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTAAAAAAATAAATAAAATAATTTTTTTTTAAAATAAAGATAAACATCAAAAAAAACAAAAAAATAAAAATATTAGAGTAGTTaaa                                                                                >  1:3342729/1‑150 (MQ=255)
                                                                         gttggtCAGCATCTGCTTTTTCGATACCACTAAAATTTCAATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                               <  1:6916272/150‑1 (MQ=255)
                                                                             ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATATAGGTAACCATCACACAAAACACAAAATTAAAATTAATAAAGGAGTGAAAGGCaa                                                                          >  1:6339838/1‑150 (MQ=255)
                                                                              tCAGCATCGGATTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  2:9024793/150‑1 (MQ=255)
                                                                                   atcttttttGTCGAAACCACGTACATTTTAATATCCTTTCGTTTTTTTTCTATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  1:3747346/144‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  2:4900311/150‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  2:3811281/150‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  2:4359391/150‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    >  2:6816844/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGAAACCACGTACATTTTAATATCCTGTCTTTTGTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  1:370077/150‑1 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATGAAATTATATTTTTTTAAATATCGAGAAAACGAAAACAAAAAACACAAAAATAAAAATATTAGAGGAGGGTAAGGCaaaaaaaag                                                                   >  2:8585030/1‑148 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAAGAAAATATTTTTTTTTAAATATAGAGATAAAGATGACAAAAACGACAATATTATAATTATTAGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCGa                                                          >  2:3171144/1‑150 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATGAAATTATTTTTTTTAAATATAGATATAAACATCACCAAAAAGACAAAATGAAAATTATTCGAGGAGTGTAATGGAAAAAAACGGCCACCCgc                                                          >  2:7820571/1‑149 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  2:3675236/150‑1 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                       <  2:4524633/150‑1 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAg                                                       >  2:6190785/1‑150 (MQ=255)
                                                                                                aTACAACTTAAATTTACCTCTCCTTTCTTTTTTTTTCTTTTAAAAAATATATTTAATTTTTTTTTTTTTAAATTTCTATATAACTAACAAAAAAACGACAATATTAAAATTTTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                       <  1:7123253/150‑1 (MQ=255)
                                                                                                 tACAACTTACATTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:8813600/150‑1 (MQ=255)
                                                                                                       gTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  2:6072528/150‑1 (MQ=255)
                                                                                                        taaaTTTCCATACCCTTCCGTTTTTTTTCGATTTCAAAAAAAAATGAAATTATTTTTTTTTAAATATCGACATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                               <  1:6655417/147‑1 (MQ=255)
                                                                                                          cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:7616214/150‑1 (MQ=255)
                                                                                                               tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  2:6280640/150‑1 (MQ=255)
                                                                                                                   atCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAATATGAAAAATAATCGAGGAGTGAAAGGCAAAAAAAAGGCCAACCGATAGGGAAGCCCTAGCAAAGTgc                                    >  2:6550477/1‑150 (MQ=255)
                                                                                                                    tCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   >  2:1458011/1‑150 (MQ=255)
                                                                                                                     ccTTTCGTTTGTTTTCGATTTAAAATTATAATGAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:6371346/150‑1 (MQ=255)
                                                                                                                       tGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTGTTTTTATATTTCGAGATAACGATGACAAAAACGTCAATTTGATAATTATTGGTGGTGTGTATGGGAATTATACGGCCTCCCGATAGGGTAGCCGTAGCTAAGTGcgcgg                                >  2:4128588/1‑149 (MQ=255)
                                                                                                                       ggTCGTTGGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                <  1:6808315/149‑1 (MQ=255)
                                                                                                                         tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              <  2:5921738/150‑1 (MQ=255)
                                                                                                                         tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              >  2:1658030/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  1:7968096/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  1:2071564/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  1:1315648/150‑1 (MQ=255)
                                                                                                                             tttttttCTATTTAAAAATATAATTAAATTATTTTTTTTTAATTATCTATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  1:2643283/147‑1 (MQ=255)
                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      <  2:9240888/150‑1 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:6947981/1‑150 (MQ=255)
                                                                                                                                   tctaTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  2:1145604/147‑1 (MQ=255)
                                                                                                                                   tctaTTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  1:3438800/147‑1 (MQ=255)
                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  1:2273147/1‑150 (MQ=255)
                                                                                                                                   tCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACTAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  1:8590135/150‑1 (MQ=255)
                                                                                                                                     gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  >  2:8456872/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  <  2:3719830/150‑1 (MQ=255)
                                                                                                                                       tttCAAAATATAATGAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                <  1:98286/150‑1 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  2:7494186/150‑1 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  2:4200771/150‑1 (MQ=255)
                                                                                                                                              atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  2:5718103/150‑1 (MQ=255)
                                                                                                                                               tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  1:5995429/150‑1 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:1728677/150‑1 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:5192595/150‑1 (MQ=255)
                                                                                                                                                   aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     <  2:3539768/150‑1 (MQ=255)
                                                                                                                                                   aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     <  1:764336/150‑1 (MQ=255)
                                                                                                                                                    aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    <  1:4482691/150‑1 (MQ=255)
                                                                                                                                                     tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   >  2:3460667/1‑150 (MQ=255)
                                                                                                                                                      gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:4651274/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: