Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 14.2% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC14.2% 538.0 / 16.6 279intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (55/148);  new base C (33/6);  total (98/181)
Fisher's exact test for biased strand distribution p-value = 1.69e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326
                                                                                                                                                     |                                                                                                                                                     
 aCTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAg                                                                                                                                                      <  2:4871243/150‑1 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCgggg                                                                                                                                                    >  2:7893389/1‑150 (MQ=255)
    ggtggtGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgggg                                                                                                                                                   <  1:7618530/150‑1 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCACTCATCCTGTTGCGCTCCTGTCATGTGCATTGCGTCATATAAACACTGGCGCAAGGAGCGCGCCgcggg                                                                                                                                                  >  2:8828936/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 >  1:6681744/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  1:1152990/150‑1 (MQ=255)
       ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                                <  2:5583688/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                               <  2:7375825/150‑1 (MQ=255)
         tgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                              >  2:7752765/1‑150 (MQ=255)
         tgACTGGCGGATGTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGACAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTCCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                              <  2:2589697/150‑1 (MQ=255)
           aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCa                                                                                                                                            <  2:1540156/150‑1 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGgggggggggcccg                                                                                                                                          >  1:124149/1‑145 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGCCCAt                                                                                                                                          >  1:4419646/1‑150 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCaaa                                                                                                                                          >  2:762185/1‑149 (MQ=255)
              tgCGTAATTAAGATGATATATTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                         <  2:859580/149‑1 (MQ=255)
              ggCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                         <  1:7110433/150‑1 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGcccaaag                                                                                                                                        >  2:5145028/1‑145 (MQ=255)
                cGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAcgc                                                                                                                                       >  2:6082788/1‑150 (MQ=255)
                cGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgc                                                                                                                                       <  1:967676/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATACTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  2:2424510/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCCAAcgcc                                                                                                                                      >  2:1023677/1‑150 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAAcgccg                                                                                                                                     >  2:7695548/1‑150 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAAagccg                                                                                                                                     >  2:1271228/1‑150 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccg                                                                                                                                     <  1:4520944/150‑1 (MQ=255)
                  tATTTAAGATGATATATTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccg                                                                                                                                     <  1:6444485/150‑1 (MQ=255)
                    tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAcgccccc                                                                                                                                   >  1:7594232/1‑150 (MQ=255)
                       aaGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                                                <  2:4174387/150‑1 (MQ=255)
                        aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGCGGCGGCGATTGgccgccccct                                                                                                                               <  2:2669327/150‑2 (MQ=39)
                         tattatatTTTAAAATTAATTAATGTAATCAGGTCCTAAAATAACTAGAATATTTCAGTCTATCATCCTGTTGCGCTCCTGTCATGTGCTTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                              <  1:7908900/146‑1 (MQ=37)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGcccccccccc                                                                                                                              >  2:6105297/1‑150 (MQ=255)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCt                                                                                                                            <  2:8481455/150‑1 (MQ=255)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCt                                                                                                                            <  1:2356890/150‑1 (MQ=255)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAAGCCGcccccccca                                                                                                                            >  2:3403883/1‑149 (MQ=255)
                           tgatATATTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCt                                                                                                                            <  2:8957057/150‑1 (MQ=255)
                            gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCCAACcccccccccccccc                                                                                                                           >  1:1920707/1‑148 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGc                                                                                                                          <  1:8375827/150‑1 (MQ=255)
                              tcttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATCTTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCa                                                                                                                         >  2:4705173/3‑150 (MQ=38)
                                ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGCCAAACACCCCCCCCCCCaccacc                                                                                                                       >  1:6002618/1‑150 (MQ=255)
                                ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAACCCCCCGCCCCCaccacc                                                                                                                       >  2:8272886/1‑150 (MQ=38)
                                 tttAAAATTATTTAATGTCATCTGGTCCTAAAATAACGAGAATATTTCAGTCTCTCATCGTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCAccc                                                                                                                      <  1:6477379/150‑1 (MQ=37)
                                 tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCCACGCCCCcgccccccgccccc                                                                                                                      >  1:5542354/1‑150 (MQ=255)
                                  ttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCAGATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCCCACGCCCCCCCCCCCACCAcccc                                                                                                                     >  1:7760238/1‑150 (MQ=38)
                                   tAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAccccc                                                                                                                    <  2:1465132/150‑1 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCAAAACCGCCCCCCCCACCAcccccccccg                                                                                                               >  2:2036323/1‑145 (MQ=255)
                                          aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCGGCACCCCCGGGcgct                                                                                                             >  2:3152529/1‑150 (MQ=255)
                                          aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGcact                                                                                                             >  2:8569332/1‑150 (MQ=255)
                                           attaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATGGCCGCCGCCCCCTGCACCCCCGGGCTCTg                                                                                                            <  2:49689/150‑1 (MQ=255)
                                           attaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGcgcag                                                                                                            >  2:9330538/1‑146 (MQ=37)
                                              aatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTTCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCCCCCCCCCCCCCACCCCCCGGCGCAGGCg                                                                                                         >  1:6932687/1‑150 (MQ=37)
                                                tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGAATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGaa                                                                                                       <  2:8659093/150‑1 (MQ=255)
                                                  tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGGGCCCAACGCCCCCCCCCCCAGCACCCCCCGGCGCTGGCGGAcc                                                                                                     >  1:3033030/1‑149 (MQ=255)
                                                    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCCATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACaaa                                                                                                   >  2:5314133/1‑150 (MQ=255)
                                                    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAAAAGCCCCCCCCCCCCGCCCCCCCCGCCCCAGGCGCACaaa                                                                                                   >  2:5641532/1‑150 (MQ=25)
                                                     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGACCaaaa                                                                                                  >  2:6104921/1‑150 (MQ=25)
                                                                 aTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGATCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCAAATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc                                                                                      <  2:7136943/150‑1 (MQ=255)
                                                                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCGCCCCCCCCAGCACCCCCCCCGGCAGGCGAACCAAAAAGCCGCCgcgccgg                                                                                   >  2:5938984/1‑147 (MQ=21)
                                                                      gagaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCACTGGCGAACAAAATCGCCGCTGCGCGGTGc                                                                                 >  2:6902338/1‑150 (MQ=255)
                                                                         aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                              <  2:2970669/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:2304837/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:5878741/150‑1 (MQ=255)
                                                                             tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                          <  2:329299/150‑1 (MQ=255)
                                                                             tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAAAAAAAAAGCCGCTGCGCGGTGCCCACGGc                                                                          >  2:551288/1‑150 (MQ=18)
                                                                              ttCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                         <  2:2629064/150‑1 (MQ=255)
                                                                              tactgTCTCTCACCCTGTTGCGCTCCTGTCATGTGATTTGATACAAATAATCCCTGGCGCACGGCGCGCGCGGGGGGCGGCCACTCGCGGCCGCCCCCTGCACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                         <  1:1564435/146‑1 (MQ=17)
                                                                                cAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                       <  2:697161/150‑1 (MQ=255)
                                                                                cAGTCTCTAATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCGGGCGAACGGAGCGCGCCGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                       <  1:6038620/150‑1 (MQ=18)
                                                                                   tctctcATCTTGTTGCGCTCGTTTCTTGTGATTTGCTAAATATAACCCGTGGCGATTGGCGCGGGCGGGGGGCGGCCATGCGCCGCCGCCCCCTGCACCCCGGGGCTCTGGCTAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  1:6104932/150‑1 (MQ=2)
                                                                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  2:3015776/150‑1 (MQ=255)
                                                                                       tcATCCTGTTGCGCTCCTGTCATGTGCTTTGATTAATATAATCACTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                <  1:4628506/150‑1 (MQ=17)
                                                                                          tCCTGTTGCGCTCCTGTCTTGTGCTTTGCTTAATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                             <  1:5792827/150‑1 (MQ=255)
                                                                                               ttGCGCTCCTGTAATGTGCTTTGCTTAATATATTCAGTGGGGCAAGGAGCGCGCTGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAcc                                                        <  1:9006039/150‑1 (MQ=17)
                                                                                                tGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCg                                                       <  2:1620078/150‑1 (MQ=255)
                                                                                                   gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCAACGCCGCCCCCCCCACCACCCCCCGGCACTGGCGAACAAAAAAGCCGCTGCGCCGGGCCCACCGCGTATACCAAAAGGCTACCGGGt                                                    >  1:7531007/1‑150 (MQ=255)
                                                                                                    cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGATCAAATTCGCCGCTGCGCGGTGCCCTGGGCTTGTCCCTGACGGGTACCGTGTc                                                   <  2:4918620/150‑1 (MQ=17)
                                                                                                       cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  2:8358213/150‑1 (MQ=255)
                                                                                                              gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  2:8034634/150‑1 (MQ=255)
                                                                                                              gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  1:7436526/150‑1 (MQ=255)
                                                                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCAAACGCCCCCCCCCCCCCCACCCCCCCGCTCAGGCGAACAAAAAACCCCCAGCGCCGTGCCCACCGCGTATACCCTAAGGCTACCCGGTCGGGCGCGCggg                                        >  2:2245201/1‑149 (MQ=255)
                                                                                                                 ctttGCTTCTTATAATCACTGGCGCTAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  1:8629159/148‑1 (MQ=255)
                                                                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  2:5286729/150‑1 (MQ=255)
                                                                                                                  aTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAAc                                     <  1:5177071/150‑1 (MQ=255)
                                                                                                                   ttGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                    <  1:8887338/150‑1 (MQ=255)
                                                                                                                      cTTCATATATTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCGTGGTCCCCCGGGCTCTGGCGATCAAAATCGCCGATGCGCGGTGCCCTCGGCTTATCCCTTACGGCTTCCGGGTCGGGGGCGTGGTATCATcc                                 <  2:7406867/150‑1 (MQ=11)
                                                                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                 <  1:6808885/150‑1 (MQ=40)
                                                                                                                       ttCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                <  2:1758124/150‑1 (MQ=40)
                                                                                                                           tataATCACTGGCGCAAGGTGCGCGCAGGGGGCGGCCAATCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa                            <  2:4031012/150‑1 (MQ=255)
                                                                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  1:6344823/150‑1 (MQ=34)
                                                                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  2:1838975/150‑1 (MQ=34)
                                                                                                                               aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  1:2645977/150‑1 (MQ=33)
                                                                                                                                 cACTGGCTCAAGGAGCGCGCTGGGGGCTGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                      <  2:69698/150‑1 (MQ=12)
                                                                                                                                  aCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  1:8186218/150‑1 (MQ=32)
                                                                                                                                   cTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:206583/150‑1 (MQ=17)
                                                                                                                                          aaGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc             <  1:2247964/150‑1 (MQ=32)
                                                                                                                                            ggAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc           <  1:3753115/150‑1 (MQ=32)
                                                                                                                                                cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCATGCGAAAAAAATAGCCGCTGCGCGGTGCCCAAGGCTTATACCTTAAGGCTACCGGGTAGGGCGCGAGGTAAAATACCAGTAAAAAGCGCCCTCAGCCCACAt       >  2:2342771/1‑150 (MQ=11)
                                                                                                                                                   gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAACCCAGTAAAACGCGCCCGCAGCCCACAGCCa    >  2:7838776/1‑150 (MQ=11)
                                                                                                                                                   gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATCCCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCGCAGCCCACATCCa    >  2:6662233/1‑150 (MQ=12)
                                                                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  2:7258934/150‑1 (MQ=32)
                                                                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  2:1113273/150‑1 (MQ=32)
                                                                                                                                                     aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  >  2:9201725/1‑150 (MQ=32)
                                                                                                                                                     |                                                                                                                                                     
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: