Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,393,847 A→C 9.2% D400E (GAT→GAG nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,393,8470AC 9.2% 1808.2 / 63.3 631D400E (GAT→GAGnuoLNADH:ubiquinone oxidoreductase, membrane subunit L
Reads supporting (aligned to +/- strand):  ref base A (288/270);  new base C (3/65);  total (296/335)
Fisher's exact test for biased strand distribution p-value = 9.03e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 642 total aligned reads displayed.

CCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGA  >  NC_000913/2393698‑2393996
                                                                                                                                                     |                                                                                                                                                     
 cTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATATACGCACCGACCAGACCTGCCACCATCAGAGTGTTATGACCCTTCGCCATCGCACCCGCGAGGATCTCAt                                                                                                                                                      >  2:1176792/1‑150 (MQ=255)
 cTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCAt                                                                                                                                                      >  1:9020297/1‑150 (MQ=255)
 cTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCAt                                                                                                                                                      >  2:2066837/1‑150 (MQ=255)
  tttCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATc                                                                                                                                                     >  1:7251197/1‑150 (MQ=255)
      aCGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTa                                                                                                                                                 <  1:8279510/150‑1 (MQ=255)
           gtgagcgtgaATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTgaa                                                                                                                                            >  1:7634777/1‑150 (MQ=255)
           gtgagcgtgaATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTgaa                                                                                                                                            <  2:1832024/150‑1 (MQ=255)
              agcgtgaATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTgaagaa                                                                                                                                         >  1:8430987/1‑150 (MQ=255)
                  tgaATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGccc                                                                                                                                     <  1:4784254/150‑1 (MQ=255)
                     aTTTGTTCTTTTCCGTGGAAGACGATTAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCa                                                                                                                                  <  1:1733286/150‑1 (MQ=255)
                       ttGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGt                                                                                                                                >  2:8976385/1‑150 (MQ=255)
                       ttGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCACACCTGCCACCATCAGATTGATCTGGCCCGTCGCCAGCGCACCCGCGAGGATCTCATCCTGACTGAGGGAGCCCGCAGt                                                                                                                                >  2:3605323/1‑150 (MQ=255)
                           tCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGAcc                                                                                                                            >  1:4013107/1‑150 (MQ=255)
                           tCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGAcc                                                                                                                            >  2:2952753/1‑150 (MQ=255)
                            cTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCa                                                                                                                           <  1:2100843/150‑1 (MQ=255)
                              tttCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGc                                                                                                                         >  1:2443840/1‑150 (MQ=255)
                              tttCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGc                                                                                                                         >  2:7953052/1‑150 (MQ=255)
                               gtCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCg                                                                                                                        <  2:9277637/149‑1 (MQ=255)
                                  ngTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTa                                                                                                                     >  1:7210081/2‑150 (MQ=255)
                                  cGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTa                                                                                                                     <  2:7388458/150‑1 (MQ=255)
                                      gAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGAGCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCTTCCTTAGTGTAGAAGCCCGCAGTGACCAGCGGTAGTGc                                                                                                                 >  2:5560282/1‑150 (MQ=255)
                                           cGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTAGTGAAGAAGCCCGCTGTGTCCAGCGGTCGTGCCGACa                                                                                                            >  2:9169043/1‑150 (MQ=255)
                                              tGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGtg                                                                                                         >  1:5649263/1‑150 (MQ=255)
                                                  aaTCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGtgctgc                                                                                                     <  2:3203235/150‑1 (MQ=255)
                                                  aaTCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGtgctgc                                                                                                     <  2:5652934/150‑1 (MQ=255)
                                                     cATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGcgcc                                                                                                  <  1:4592720/150‑1 (MQ=255)
                                                      aTACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGcgccg                                                                                                 >  1:2262196/1‑150 (MQ=255)
                                                       tACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGcgccgc                                                                                                <  1:2378965/150‑1 (MQ=255)
                                                        aCGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCgccgcc                                                                                               <  1:3028617/150‑1 (MQ=255)
                                                        aCGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCgccgcc                                                                                               <  1:3553312/150‑1 (MQ=255)
                                                          ggAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCcca                                                                                             <  2:4650269/150‑1 (MQ=255)
                                                          ggAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCcca                                                                                             >  1:3854527/1‑150 (MQ=255)
                                                                 gtAGAGCGAGGTCATAAACGCACCGACCAGTCCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGaa                                                                                      <  2:7135493/150‑1 (MQ=255)
                                                                 gtAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGaa                                                                                      <  1:5814603/150‑1 (MQ=255)
                                                                 gtAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGaa                                                                                      >  1:3160827/1‑150 (MQ=255)
                                                                 gtAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCACGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGaa                                                                                      >  2:3069291/1‑150 (MQ=255)
                                                                  tAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAg                                                                                     <  1:7054475/150‑1 (MQ=255)
                                                                  tAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAg                                                                                     <  2:2262603/150‑1 (MQ=255)
                                                                     agCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGTGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCag                                                                                  <  1:6138773/150‑1 (MQ=255)
                                                                     agCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCag                                                                                  >  1:4114579/1‑150 (MQ=255)
                                                                     agCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCag                                                                                  <  1:2876949/150‑1 (MQ=255)
                                                                      gCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCaga                                                                                 <  2:4544202/150‑1 (MQ=255)
                                                                        gAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAgaga                                                                               >  2:7446921/1‑150 (MQ=255)
                                                                          ggTCATAACCGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATa                                                                             <  1:2559052/150‑1 (MQ=255)
                                                                           gTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATaa                                                                            >  1:4458679/1‑150 (MQ=255)
                                                                            tCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATaaa                                                                           >  1:1281886/1‑150 (MQ=255)
                                                                             cATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAAc                                                                          <  2:65858/150‑1 (MQ=255)
                                                                              aTAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAAcc                                                                         >  1:8016138/1‑150 (MQ=255)
                                                                               tAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCa                                                                        <  1:7506399/150‑1 (MQ=255)
                                                                               tAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCa                                                                        <  2:5990199/150‑1 (MQ=255)
                                                                                aaaCGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAg                                                                       >  1:1383599/1‑150 (MQ=255)
                                                                                  aCGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCg                                                                     >  1:9191337/1‑150 (MQ=255)
                                                                                     cACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGaa                                                                  >  2:4897256/1‑150 (MQ=255)
                                                                                      aCCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGa                                                                   <  2:8774469/148‑1 (MQ=255)
                                                                                      aCCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAAt                                                                 >  1:6509410/1‑150 (MQ=255)
                                                                                       ccGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATa                                                                <  1:1560150/150‑1 (MQ=255)
                                                                                       ccGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATa                                                                <  2:5136103/150‑1 (MQ=255)
                                                                                        cGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAg                                                               >  2:8458244/1‑150 (MQ=255)
                                                                                         gACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGa                                                              >  1:5225873/1‑150 (MQ=255)
                                                                                         gACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGa                                                              <  1:814523/150‑1 (MQ=255)
                                                                                          aCCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGAGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGAt                                                             >  1:9325369/1‑150 (MQ=255)
                                                                                           ccAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGAtt                                                            <  1:674115/150‑1 (MQ=255)
                                                                                           ccAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGAtt                                                            >  2:5307524/1‑150 (MQ=255)
                                                                                            cAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGAttt                                                           <  2:6301975/150‑1 (MQ=255)
                                                                                                     caccaTCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGAcc                                                  >  2:4131155/1‑150 (MQ=255)
                                                                                                      accaTCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCg                                                 <  2:164457/150‑1 (MQ=255)
                                                                                                            aGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATc                                           <  2:859593/150‑1 (MQ=255)
                                                                                                             gATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCt                                          <  1:1007096/150‑1 (MQ=255)
                                                                                                              aTTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCtt                                         <  1:4341388/150‑1 (MQ=255)
                                                                                                                 gATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGaa                                      <  1:6876985/150‑1 (MQ=255)
                                                                                                                 gATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGaa                                      <  1:7902501/150‑1 (MQ=255)
                                                                                                                      gACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgt                                 >  2:827816/1‑150 (MQ=255)
                                                                                                                      gACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgt                                 <  2:2703595/150‑1 (MQ=255)
                                                                                                                       aCCAGTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgtt                                <  1:7848829/150‑1 (MQ=255)
                                                                                                                        ccATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttc                               <  1:4220258/150‑1 (MQ=255)
                                                                                                                        ccATTCGCCATCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttc                               <  1:4629013/150‑1 (MQ=255)
                                                                                                                         cATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttct                              <  1:5560282/150‑1 (MQ=255)
                                                                                                                         cATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttct                              <  2:7577855/150‑1 (MQ=255)
                                                                                                                          aTTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttctg                             >  2:6420732/1‑150 (MQ=255)
                                                                                                                           ttCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttctgt                            <  1:9150624/150‑1 (MQ=255)
                                                                                                                            tCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttctgtt                           <  2:3957017/150‑1 (MQ=255)
                                                                                                                            tCGCCAACGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCACGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttctgtt                           <  2:5484148/150‑1 (MQ=255)
                                                                                                                             cGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGAtgttctgttc                          <  1:3069351/150‑1 (MQ=255)
                                                                                                                              gCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCg                         >  1:53647/1‑150 (MQ=255)
                                                                                                                               ccATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGt                        <  2:1934812/150‑1 (MQ=255)
                                                                                                                                 aTCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGa                      <  1:4925469/150‑1 (MQ=255)
                                                                                                                                 aTCGCACCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGa                      <  1:189854/150‑1 (MQ=255)
                                                                                                                                 aTCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGa                      <  2:6821568/150‑1 (MQ=255)
                                                                                                                                  atggcaccGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGAt                     >  1:5998031/7‑150 (MQ=255)
                                                                                                                                     cACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGc                  >  2:5002420/1‑150 (MQ=255)
                                                                                                                                      aCCCGCGAGGATCTCCTCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCa                 <  1:3436401/150‑1 (MQ=255)
                                                                                                                                        ccGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAgg               >  2:1470901/1‑150 (MQ=255)
                                                                                                                                            gAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAg           >  2:3470648/1‑150 (MQ=255)
                                                                                                                                            gAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAg           <  2:3834732/150‑1 (MQ=255)
                                                                                                                                            gAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAg           <  2:6692603/150‑1 (MQ=255)
                                                                                                                                              ggATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGaa         <  1:8795402/150‑1 (MQ=255)
                                                                                                                                                  ctcctcCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGAc     <  1:6321123/150‑1 (MQ=255)
                                                                                                                                                  ctcATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGAc     <  2:7187093/150‑1 (MQ=255)
                                                                                                                                                   tcATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAg                                                                                     <  2:2530483/69‑1 (MQ=255)
                                                                                                                                                   tcATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACg    <  1:8503893/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCATTCGCCATCGCACCCGCGAGGATCTCATCCTTACTGAAGAAGCCCGCAGTGACCAGCGGTAGTGCCGACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGA  >  NC_000913/2393698‑2393996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: