Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,241 C→A 7.0% A38S (GCA→TCA)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,2410CA 7.0% 1710.5 / 21.7 519A38S (GCA→TCA) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (296/170);  new base A (2/51);  total (298/221)
Fisher's exact test for biased strand distribution p-value = 1.69e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 533 total aligned reads displayed.

CGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT  >  NC_000913/2411092‑2411390
                                                                                                                                                     |                                                                                                                                                     
cGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGc                                                                                                                                                       >  2:1465181/1‑150 (MQ=255)
cGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGc                                                                                                                                                       >  2:2126196/1‑150 (MQ=255)
      tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcgc                                                                                                                                                 >  1:1462807/1‑150 (MQ=255)
      tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcgc                                                                                                                                                 >  1:8258933/1‑150 (MQ=255)
         gACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGAc                                                                                                                                              <  1:6975875/150‑1 (MQ=255)
         gACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGAc                                                                                                                                              <  2:5996614/150‑1 (MQ=255)
           ccAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACaa                                                                                                                                            >  2:7782453/1‑150 (MQ=255)
           ccAGCCATGTGCCCATTACGGTGGCAACACGGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGTACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACaa                                                                                                                                            <  1:8017865/150‑1 (MQ=255)
             aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCTGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATa                                                                                                                                          <  1:5577318/150‑1 (MQ=255)
             aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGGTCCGCTTAATGCAAACGCGACACTa                                                                                                                                          >  2:6315763/1‑150 (MQ=255)
              gCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaa                                                                                                                                         >  2:872478/1‑150 (MQ=255)
               ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACACGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                                                                                                                                        <  1:1074389/150‑1 (MQ=255)
               ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                                                                                                                                        >  1:3573475/1‑150 (MQ=255)
               ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                                                                                                                                        <  2:137581/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATACACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  1:6624206/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  1:8467901/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  1:2299912/150‑1 (MQ=255)
                 aTGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaa                                                                                                                                      >  2:6748956/1‑150 (MQ=255)
                   gTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaaac                                                                                                                                    >  1:9271554/1‑150 (MQ=255)
                     tCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAacaaacaa                                                                                                                                  <  2:2612099/150‑1 (MQ=255)
                       ccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATGCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAg                                                                                                                                >  1:4710058/1‑150 (MQ=255)
                        cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                               <  2:2691230/150‑1 (MQ=255)
                        cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                               >  1:9161295/1‑150 (MQ=255)
                              ggTGGAAACACCGGTACGGACAAAACCATCGCAAATAATAAACAAGGCGGAAATCAGTACAACGTTTGGATCATAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAAACGCTACAATAAAAAACAAAGCGACCGc                                                                                                                         <  2:8881184/150‑1 (MQ=255)
                                 gtcAACACCGTTAGGTACCAAACAATCGAAAATAATAAACATGGGGGAAATCTGGACAAGTTTTGTATCATAAAAGCCTTAAAATACTTCTGGGACTGTAAGCGTTCCGCTTAATGAAAACGCTAAAATAACAAACAAAGCGACCGCATc                                                                                                                      <  2:8253329/148‑1 (MQ=255)
                                   cAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCa                                                                                                                    >  2:9289979/1‑150 (MQ=255)
                                    aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAt                                                                                                                   >  2:5655501/1‑150 (MQ=255)
                                    aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAt                                                                                                                   >  2:3808803/1‑150 (MQ=255)
                                       acCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACg                                                                                                                >  2:5263581/1‑150 (MQ=255)
                                         cGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCa                                                                                                              >  2:2423958/1‑150 (MQ=255)
                                               ggACCAACCCTGCGCCAATAATAACCACGGCGGCAATCAGGACACCGTTCGGATCAGAAAAGCGGGAAAATACTTGTGGGACTGTCAGCGTTCCGCTTATTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGc                                                                                                        <  2:2915651/150‑1 (MQ=255)
                                                  ccAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCg                                                                                                     >  2:5771861/1‑150 (MQ=255)
                                                  ccAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCg                                                                                                     >  1:2129644/1‑150 (MQ=255)
                                                   aaaaCAATCTCAAATAATAAACATGGCGGAAATCTGTACAACTTTTGTATCATAAATGCGGTAAAATACTTCTGGTACTGTCAGCGTTCCGATTATTGAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGt                                                                                                    <  2:1121352/149‑1 (MQ=255)
                                                     aaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCg                                                                                                  >  2:813744/1‑150 (MQ=255)
                                                     aaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCg                                                                                                  >  2:1286337/1‑150 (MQ=255)
                                                        cATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaa                                                                                               >  1:2485335/1‑150 (MQ=255)
                                                         aTCGCAAATAATAACCAAGGCGGAAATCTGGAAAACGTTAGTAAAATAAAAGCCGTAAATTACTTCTGGGACTGTCAGCTTTCCGTTTAATCCAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaaa                                                                                              <  2:6452468/150‑1 (MQ=255)
                                                          tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAAc                                                                                             >  1:4532899/1‑150 (MQ=255)
                                                            gCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                                                           >  2:2486946/1‑150 (MQ=255)
                                                               aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                        >  2:9278243/1‑150 (MQ=255)
                                                                 taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGa                                                                                      >  1:4110683/1‑150 (MQ=255)
                                                                         aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACg                                                                              >  2:7629806/1‑150 (MQ=255)
                                                                         aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACg                                                                              >  2:705235/1‑150 (MQ=255)
                                                                          aGGCGGAAATCAGGACAAAGTTAGTATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCTGCGTTCCGATTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACgg                                                                             <  2:7492144/150‑1 (MQ=255)
                                                                                  aTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                     >  2:4419627/1‑150 (MQ=255)
                                                                                  aTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                     <  2:6233747/150‑1 (MQ=255)
                                                                                   tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                    >  1:9013258/1‑150 (MQ=255)
                                                                                      ggACAACTTTCGTATAAGAAAAGCCGTAAAATACTTGGGGTACTTTCTGGGTTCCGCTTAATGAAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaaa                                                                 <  2:3325111/150‑1 (MQ=255)
                                                                                       gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCCGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                >  2:4655011/1‑150 (MQ=255)
                                                                                        aCAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGa                                                               >  2:1712933/1‑150 (MQ=255)
                                                                                         cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                              >  1:3746901/1‑150 (MQ=255)
                                                                                           actttAGGATCATAAAAGCCGTAAAATAATTCTGGTACTGTCAGCTTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                                                            <  2:4897648/147‑1 (MQ=255)
                                                                                             gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAc                                                             <  2:9130175/147‑1 (MQ=255)
                                                                                             gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAAAGGCCAGTAATGAAAGAACCCa                                                          >  2:396580/1‑150 (MQ=255)
                                                                                                agtaTCATAAACGCCTTAAAATTCTTGTGGGTGTTTCCGGGTTCCGTTTAATCAAACCGCTAAAATAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                       <  1:17079/147‑1 (MQ=255)
                                                                                                aGGATCAGAAAAGCGGGAAAATTCTTCTTGGACTGTCAGCGTTCCGCTTTATGCAAACGCTACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                       <  2:6863827/150‑1 (MQ=255)
                                                                                                aGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                       <  2:6969369/150‑1 (MQ=255)
                                                                                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                     >  1:524023/1‑150 (MQ=255)
                                                                                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                     >  1:630961/1‑150 (MQ=255)
                                                                                                   aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAAAAACAAACAAAGCGACCGCATCCATACGAACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                    >  1:3154690/1‑150 (MQ=255)
                                                                                                    tCAGAAAAGCCGTAAAATCCTTCTGGTACTGTCTGCGTTCGGATTATTGAAACCGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                   <  1:3832822/150‑1 (MQ=255)
                                                                                                     cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                  >  1:5681926/1‑150 (MQ=255)
                                                                                                            gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                           <  2:1564890/150‑1 (MQ=255)
                                                                                                             ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                          >  1:822529/1‑150 (MQ=255)
                                                                                                             ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                          >  2:3470349/1‑150 (MQ=255)
                                                                                                              cttaaaaTAATTGTGGGACTTTCTGGGTTCCTATTAATTAAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                         <  2:4136390/147‑1 (MQ=255)
                                                                                                              cGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                         >  1:596951/1‑150 (MQ=255)
                                                                                                                gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                       >  2:7223564/1‑150 (MQ=255)
                                                                                                                 aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                      <  1:5385162/150‑1 (MQ=255)
                                                                                                                     tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                                  >  2:5565133/1‑150 (MQ=255)
                                                                                                                       cTTCTGGTAGTGTCAGGTTTCCGCTTAATGCAAAGGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                <  1:4927716/150‑1 (MQ=255)
                                                                                                                       cTTCTGGGAGTGTCCGCGGTCCGCTTCATGCAACCGCGACAATCACAACCACCGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                <  2:4988591/150‑1 (MQ=255)
                                                                                                                       cTTCTGGGACTGTCCGCGTTCCGATTAATGCAAACGCCACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                <  1:6414510/150‑1 (MQ=255)
                                                                                                                       cTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                >  2:4790591/1‑150 (MQ=255)
                                                                                                                        ttCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                               >  1:4356931/1‑150 (MQ=255)
                                                                                                                        ttCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                               >  2:2021460/1‑150 (MQ=255)
                                                                                                                        ttCTGGGACTGTCAGCGTTCCGATTAATGAAAACGCGAAAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                               <  1:2948611/150‑1 (MQ=255)
                                                                                                                          cTGGGACTGTCAGCTTCCCGCTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                             <  1:6699004/150‑1 (MQ=255)
                                                                                                                          cTGGCAATGTCAGCGTTCCGATTAATCAAAACGCTAAAATAAAAAACAAAGCGACCCCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                             <  2:3695116/150‑1 (MQ=255)
                                                                                                                           tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                            >  2:4526191/1‑150 (MQ=255)
                                                                                                                           tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                            >  2:2541056/1‑150 (MQ=255)
                                                                                                                               agtGTCCGCGTTCCGCTTATTCCAAACGCTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTaa                        <  1:8739485/148‑1 (MQ=255)
                                                                                                                               aCTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTaa                        >  1:723527/1‑150 (MQ=255)
                                                                                                                                   tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                    >  2:2770331/1‑150 (MQ=255)
                                                                                                                                   tCAGCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                    <  2:8438059/150‑1 (MQ=255)
                                                                                                                                    cAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                   <  2:6534509/150‑1 (MQ=255)
                                                                                                                                    cAGCGTGCCGCTTAATGCAACCGCCACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                   <  1:6837275/150‑1 (MQ=255)
                                                                                                                                     aGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                  >  1:7522895/1‑150 (MQ=255)
                                                                                                                                      gCGTTCCGATTAATCAAAACGCCACAAAAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                 <  1:7868294/150‑1 (MQ=255)
                                                                                                                                      gCGTTCCGATTAACCCACACGCTACAACAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                 <  1:8589842/150‑1 (MQ=255)
                                                                                                                                       cGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg                >  2:2329860/1‑150 (MQ=255)
                                                                                                                                       cGTTCCGCTTAAGGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg                <  2:188961/150‑1 (MQ=255)
                                                                                                                                        gTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg               >  1:7809103/1‑150 (MQ=255)
                                                                                                                                          tCCTCTTAATCAAAACGCCAAAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt             <  2:7994920/150‑1 (MQ=255)
                                                                                                                                             gCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc          >  1:1895349/1‑150 (MQ=255)
                                                                                                                                             gCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc          >  2:4303012/1‑150 (MQ=255)
                                                                                                                                              cTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt         >  1:5073233/1‑150 (MQ=255)
                                                                                                                                                   tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGagt    >  1:8842253/1‑148 (MQ=255)
                                                                                                                                                     cAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCt  >  2:311191/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT  >  NC_000913/2411092‑2411390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑