Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 11.5% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT11.5% 946.0 / 13.5 346R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (134/157);  new base T (33/7);  total (182/164)
Fisher's exact test for biased strand distribution p-value = 1.41e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

TATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470284‑2470580
                                                                                                                                                   |                                                                                                                                                     
taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATaa                                                                                                                                                     >  2:4888849/1‑150 (MQ=255)
  tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                                   <  1:6074398/150‑1 (MQ=255)
  tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATaaaa                                                                                                                                                   >  1:8009310/1‑149 (MQ=255)
    tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCa                                                                                                                                                 >  1:343597/1‑150 (MQ=255)
      tattaggAATATCACAATTTTATGTGTCATTTTTCGGGCAAGGATATAGAGATATAAGCAAGCATTTATTTGGAATGTATATTTCGTTCGACCTTTGCTTATACATAACAGTCGATTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                               <  2:3896384/145‑1 (MQ=255)
         ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATAAAAAAAAAACAAGACa                                                                                                                                            >  2:2968114/1‑150 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         <  2:3685360/150‑1 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         <  2:2924754/150‑1 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAAAACaaaa                                                                                                                                         >  1:3309698/1‑148 (MQ=255)
                atCTCAATTTTATGTGTCATTTTTCGGGGAATGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  1:4169623/150‑1 (MQ=255)
                 tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAGGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  2:2143971/150‑1 (MQ=255)
                 tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  2:5193224/150‑1 (MQ=255)
                 tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  2:606453/150‑1 (MQ=255)
                   tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                  <  1:1545621/150‑1 (MQ=255)
                    cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAATAAAAAAAAACAAAAGAGATTTa                                                                                                                                 >  2:1583613/1‑150 (MQ=255)
                       ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  2:4493863/150‑1 (MQ=255)
                       ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGGGATGTAAAg                                                                                                                              >  2:7364058/1‑150 (MQ=255)
                         ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  2:3709959/150‑1 (MQ=255)
                         ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  2:102942/150‑1 (MQ=255)
                           aTGTGTCATTTTTCGGGGACGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGAAGTAAAGCACt                                                                                                                          >  2:7041348/1‑150 (MQ=255)
                             ttgtCATTTTTCGGGGAAGGAAATAGACATTTAAGCAAGCATATATTTGGAATGTATTTTTCGATCGACCTTTGCTTATACATAACAGTCGATTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                        <  2:2414039/149‑1 (MQ=255)
                              tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATTAAATAATTAAAATAAAAAAAAAAAAAAACGATGTAAAgaaaaaag                                                                                                                       >  1:789886/1‑143 (MQ=255)
                                tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATGCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAATAGAGATGTAAAGAACAAAGtt                                                                                                                     >  2:2778797/1‑150 (MQ=255)
                                tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                     <  1:6355288/150‑1 (MQ=255)
                                  aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACAAAGTTTa                                                                                                                   >  2:9071558/1‑150 (MQ=255)
                                  aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATGTAAATCAATAAGTTTa                                                                                                                   >  2:5856527/1‑150 (MQ=255)
                                  aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAATACAATAGCGATTTTAATAACTAAGTTTa                                                                                                                   >  1:6607367/1‑150 (MQ=255)
                                  aTTTTTCGGGGAAGGATACAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTa                                                                                                                   >  2:8298238/1‑150 (MQ=255)
                                   tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                  <  2:868977/150‑1 (MQ=255)
                                                gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAAAAATACAAAAAAAAAAATACAAAAGCGATGTAAAGCCCGAAGTTTAAATTTCGCGCCaca                                                                                                     >  1:3640324/1‑148 (MQ=255)
                                                     agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTGTAAATTGCGCGCCAAACATgg                                                                                                >  2:6961666/1‑150 (MQ=255)
                                                      gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                               <  1:5462382/150‑1 (MQ=255)
                                                        gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  1:2298884/150‑1 (MQ=255)
                                                         aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTCAAAGAAATAAAGACAATAGGGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATTgcgcg                                                                                            >  2:7483977/1‑150 (MQ=255)
                                                         aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAATCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAATCAAGgcgcg                                                                                            >  2:9110655/1‑150 (MQ=255)
                                                         aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTAATTATAAAATAATTCAAAAAAATAAAAAAAAAAAAGATGTGAAGCACAAAGTTTATAATTAGCGCCACTCAGTgcgcg                                                                                            >  1:7069847/1‑150 (MQ=255)
                                                           ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                          <  2:3990174/150‑1 (MQ=255)
                                                              agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  2:8846026/150‑1 (MQ=255)
                                                              agcaagcaTACATGTGGAATGTATACTTCGTTCGACCATCCCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTCAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  2:5733250/150‑1 (MQ=255)
                                                               gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                      <  2:6359145/150‑1 (MQ=255)
                                                                caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAGc                                                                                     >  2:8562758/1‑150 (MQ=255)
                                                                caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATACAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGc                                                                                     >  2:1946160/1‑150 (MQ=255)
                                                                 aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAAATAAAAAAAAAAAAAACAAAATCGATTTAAAAAACAAAATTTAAATTTCCCGCCAATCAATGCGCGCACaaacg                                                                                    >  2:2508478/1‑147 (MQ=255)
                                                                  agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaatttaaa                                                                                   <  2:4070345/150‑8 (MQ=255)
                                                                  agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAAAAAAAAAAAACAAAAACGAAGAAAAACACAAACTTTAAAAAGAGCGCCAAAAAAGGCCCCCACaaccaa                                                                                   >  2:4232925/1‑146 (MQ=255)
                                                                       ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAATAAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTTAGCGCCAATCATGGCGCGCACAAGCTataata                                                                              >  2:1991038/1‑150 (MQ=255)
                                                                         atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                            <  2:3577981/150‑1 (MQ=255)
                                                                           ttGTAATGTATTTTTCGTTCGACATTTTATTATAAATAACAGTGTTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATTTAAAGCACTATTTTTAAATTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCaa                                                                          <  1:1277256/150‑1 (MQ=255)
                                                                            tGGAATGTATTTTTCTTTCTACCTTTGCTTATACATCACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAACGCACTAAGTTTAATTTGCGCGCCATTCATGGCGCGCACAAGCTATAATACCAAc                                                                         <  1:9122518/150‑1 (MQ=255)
                                                                              gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAAAAGCGATGTAAATCACAAATTTTAAATTTCGCGCCAATAATTGCGCGCACAAGCTATAAAACCaaaca                                                                       >  2:3784708/1‑147 (MQ=255)
                                                                                 tttATTTTTCTTTCGACATTTGATTATAAAAAACATTCTTTTTTTAAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCTATGTAAAGCAATAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                    <  1:2657780/148‑1 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                <  2:8067004/150‑1 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                <  2:4660661/150‑1 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                <  2:319447/150‑1 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAACAAGAAAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAAGCATGGCGCGCACCAGCTATAATAACAACCAAAattaa                                                                >  2:2607175/1‑148 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTATTTTAATTTAAAAAATAATTCAAATAAATCAAAAAAATAGCGAAGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATTGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                >  2:6197766/1‑150 (MQ=255)
                                                                                      ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  2:7268843/150‑1 (MQ=255)
                                                                                       tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                              <  2:2173701/150‑1 (MQ=255)
                                                                                       tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGGTTAAATTTCGCGCCCAGCATGGCGCGCCCAAGCTCTAATACCCACCTAAGTTctcc                                                              >  2:5169010/1‑150 (MQ=255)
                                                                                         tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                            <  2:4445503/150‑1 (MQ=255)
                                                                                              cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                       <  1:1029310/150‑1 (MQ=255)
                                                                                                 ccTTTGCTTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                    <  1:588149/150‑1 (MQ=255)
                                                                                                  cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATGAAGTCAATAGAGATTTAAATCACTAAGTTTAAATTTAGCGCCAATCATGGGGCGCACAAGCTATAATAGAAAACTAATTTCTGCTCGTCTTattg                                                   >  2:1370700/1‑147 (MQ=255)
                                                                                                  cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                                   >  2:5385166/1‑150 (MQ=255)
                                                                                                  cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGGTGTAAAGCACTAAGTTTAAATTGCGCGCCAAACATGGCGCGCACAACCTATAATAACAACCAAATTTCTACTCCACTTagag                                                   >  2:3601197/1‑150 (MQ=255)
                                                                                                     tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                <  2:4590043/150‑1 (MQ=255)
                                                                                                     tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAAACAAGGCGCGCACAAGCTATAAAACCAACCAAAATTCTCCTACACGTAGAGTGa                                                >  2:5038701/1‑150 (MQ=255)
                                                                                                       cTTATACATAACAGTAGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                              <  1:1789240/150‑1 (MQ=255)
                                                                                                            aCATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAAAAACACAAAATCGATGTAAATCACAAAGTTTAAAATTCGCCCCCAAAAATGCGCGCCCCAACAATAATAACAAACAAAAATCTACACCACGTAGAGGGACgataac                                         >  2:30883/1‑148 (MQ=255)
                                                                                                             cATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAACACAATAGCGCTGTAAACCAATAATTTTAAATTTCGCGCCAATAATGGCGCGCACAAGCTATCATACCCACCTCAGTTCTCCTCCTCTTTGAGTGACGATAtct                                        >  2:6665952/1‑150 (MQ=255)
                                                                                                              aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                       <  2:1184368/150‑1 (MQ=255)
                                                                                                                     tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATat                                >  2:838376/1‑149 (MQ=255)
                                                                                                                     tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                >  2:5558794/1‑150 (MQ=255)
                                                                                                                        tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCGACTCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATATCCTGATAGAAt                             >  1:6844368/1‑150 (MQ=255)
                                                                                                                        tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:8950697/1‑150 (MQ=255)
                                                                                                                         ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                            >  1:8426977/1‑150 (MQ=255)
                                                                                                                           ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                          >  1:5220820/1‑150 (MQ=255)
                                                                                                                           ttttAATATATAAAATAATACAAATAAATCAACACAATAGCGATGTAAAGCACTAAGTTTAAATTCCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                          <  1:504888/150‑1 (MQ=255)
                                                                                                                                aTTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     <  2:1756437/150‑1 (MQ=255)
                                                                                                                                  ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                   >  2:5953781/1‑150 (MQ=255)
                                                                                                                                   tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  <  1:4361681/150‑1 (MQ=255)
                                                                                                                                   tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:4988256/1‑150 (MQ=255)
                                                                                                                                   tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:1425321/1‑150 (MQ=255)
                                                                                                                                    ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAACGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 <  1:7054151/150‑1 (MQ=255)
                                                                                                                                     taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                >  2:1536726/1‑150 (MQ=255)
                                                                                                                                        aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             >  1:4605805/1‑150 (MQ=255)
                                                                                                                                         ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa            >  2:235816/1‑150 (MQ=255)
                                                                                                                                          taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa           <  1:8272754/150‑1 (MQ=255)
                                                                                                                                            atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt         >  2:7667264/1‑150 (MQ=255)
                                                                                                                                              tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       <  2:6804397/150‑1 (MQ=255)
                                                                                                                                               cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      <  2:1671959/150‑1 (MQ=255)
                                                                                                                                               cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      <  1:1927945/150‑1 (MQ=255)
                                                                                                                                                aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:477334/1‑150 (MQ=255)
                                                                                                                                                aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:3937480/1‑150 (MQ=255)
                                                                                                                                                aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:3973060/1‑150 (MQ=255)
                                                                                                                                                aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:4514077/1‑150 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCTCTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  2:7871583/150‑1 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:3518668/150‑1 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:2607175/150‑1 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  1:7371343/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470284‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: