Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 15.8% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA15.8% 953.1 / 54.3 352Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (122/164);  new base A (63/2);  total (186/166)
Fisher's exact test for biased strand distribution p-value = 6.22e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585
                                                                                                                                                    |                                                                                                                                                     
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  2:2691204/150‑1 (MQ=255)
  tattaggAATATCACAATTTTATGTGTCATTTTTCGGGCAAGGATATAGAGATATAAGCAAGCATTTATTTGGAATGTATATTTCGTTCGACCTTTGCTTATACATAACAGTCGATTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                                    <  2:3896384/145‑1 (MQ=255)
    tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAc                                                                                                                                                  >  1:7668637/1‑150 (MQ=255)
    tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGAAAGCATTTATTTGGAATGTATTTTTCTTTAGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAGaa                                                                                                                                                  >  1:5176320/1‑149 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAAAACaaaa                                                                                                                                              >  1:3309698/1‑148 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAGGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:2143971/150‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:5193224/150‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  1:7051795/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                       <  1:1545621/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAATACAAAAGCGATGt                                                                                                                                       >  2:4483635/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAAAAAAAAAAACAATAGCGATGt                                                                                                                                       >  2:2425891/1‑150 (MQ=255)
                cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAATAAAAAAAAACAAAAGAGATTTa                                                                                                                                      >  2:1583613/1‑150 (MQ=255)
                 aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAACAATACAAAAACGATGTaa                                                                                                                                     >  2:1675944/1‑150 (MQ=255)
                  aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                    <  2:6188296/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTATAg                                                                                                                                   <  2:5716163/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGGGATGTAAAg                                                                                                                                   >  2:7364058/1‑150 (MQ=255)
                     ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                                 <  2:3709959/150‑1 (MQ=255)
                       aTGTGTCATTTTTCGGGGACGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGAAGTAAAGCACt                                                                                                                               >  2:7041348/1‑150 (MQ=255)
                         gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                             <  2:6250539/150‑1 (MQ=255)
                           gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                           >  2:4561347/1‑150 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                          <  1:6355228/150‑1 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAATAAATAAAGACAATAGAGATGTAAATCACTAAGtt                                                                                                                          >  2:5892625/1‑150 (MQ=255)
                              aTTTTTCGGGGAAGGATACAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTa                                                                                                                        >  2:8298238/1‑150 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                       <  2:5737215/150‑1 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                       <  2:9099700/150‑1 (MQ=255)
                                 tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAATAAAAAAAGAAAAAATAGATTTAAATCAAGAAGTTTAAAt                                                                                                                     >  1:4220850/1‑150 (MQ=255)
                                    cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                                  <  2:105033/150‑1 (MQ=255)
                                       ggACGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGACAAAAGCGATTTAAAGCACTAAGTTTAAAATgcgcg                                                                                                               >  2:539389/1‑150 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                      <  1:6562416/150‑1 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                      <  1:4752398/150‑1 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAATAAAAAAATACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCCATccag                                                                                                      >  1:5931940/1‑147 (MQ=255)
                                                 agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTGTAAATTGCGCGCCAAACATgg                                                                                                     >  2:6961666/1‑150 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTATTAATTCATAAAATAAATAAAAGAAAACAAGACAAAAGCGAGGTAAACCACGAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                    >  2:6442240/1‑150 (MQ=255)
                                                     aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTCAAAGAAATAAAGACAATAGGGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATTgcgcg                                                                                                 >  2:7483977/1‑150 (MQ=255)
                                                     aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTAATTATAAAATAATTCAAAAAAATAAAAAAAAAAAAGATGTGAAGCACAAAGTTTATAATTAGCGCCACTCAGTgcgcg                                                                                                 >  1:7069847/1‑150 (MQ=255)
                                                       ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                               >  2:2215783/1‑150 (MQ=255)
                                                           gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                           <  2:358228/150‑1 (MQ=255)
                                                           gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAAAAAAGACAAAAGCGATTTAAAGCACTAAGTTTAAATTTCGCGCCAATAATGGCGCGCACaaa                                                                                           >  2:3472158/1‑149 (MQ=255)
                                                            caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAGc                                                                                          >  2:8562758/1‑150 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                                         <  2:8834613/150‑1 (MQ=255)
                                                              agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaatttaaa                                                                                        <  2:4070345/150‑8 (MQ=255)
                                                                  tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                                    >  2:3090477/1‑150 (MQ=255)
                                                                   ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAGAAAATAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                                   >  2:6676765/1‑150 (MQ=255)
                                                                    tatttTGAATGTATTTTTCTTTCGACCTTTTATTATAAATAACTGTAGTTTTTTTAATTTATATAATATTTCTAAGAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGAACAAGCTATAATAc                                                                                  <  2:1961243/150‑1 (MQ=255)
                                                                             tttATTTTTCTTTCGACATTTGATTATAAAAAACATTCTTTTTTTAAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCTATGTAAAGCAATAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                         <  1:2657780/148‑1 (MQ=255)
                                                                                 tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                     <  2:4660661/150‑1 (MQ=255)
                                                                                 tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                     <  2:8067004/150‑1 (MQ=255)
                                                                                  ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  2:7268843/150‑1 (MQ=255)
                                                                                     tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                                 <  2:5998993/150‑1 (MQ=255)
                                                                                     tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                                 <  2:4445503/150‑1 (MQ=255)
                                                                                       gttcgACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                               <  1:7163299/150‑1 (MQ=255)
                                                                                          cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                            <  1:5314782/150‑1 (MQ=255)
                                                                                          cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                            <  1:1029310/150‑1 (MQ=255)
                                                                                              cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATGAAGTCAATAGAGATTTAAATCACTAAGTTTAAATTTAGCGCCAATCATGGGGCGCACAAGCTATAATAGAAAACTAATTTCTGCTCGTCTTattg                                                        >  2:1370700/1‑147 (MQ=255)
                                                                                              cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATAATTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCTATAATAACAACCTAATTTCTACTCCACCTagat                                                        >  2:2955533/1‑149 (MQ=255)
                                                                                              cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAAGCAATAAGTTTAAATTGAGCGCCAAACAAGGCGCGCACAAGCAATAATACCAACCAAATTTCTCCTACTCTAagag                                                        >  2:3663841/1‑150 (MQ=255)
                                                                                               tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAATAGCGAAGTAAAGCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCTAAAAAACCAACCAAATTTCTCCACCACATAGAGt                                                       >  2:8978569/1‑150 (MQ=255)
                                                                                                 tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAAACAAGGCGCGCACAAGCTATAAAACCAACCAAAATTCTCCTACACGTAGAGTGa                                                     >  2:5038701/1‑150 (MQ=255)
                                                                                                   cTTATACATAACAGTAGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                   <  1:1789240/150‑1 (MQ=255)
                                                                                                         cATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAAAAAAATCGATTTAAAGCACAAAGTTTAAATTTCGCGCCAATAAAGGCGCGCACAAGCAAAAAAACCAACCAAAATTATACTACACTTATATTGAAAatatat                                             >  2:3932983/1‑148 (MQ=255)
                                                                                                          aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                            <  2:1184368/150‑1 (MQ=255)
                                                                                                             aaattCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAATCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTg                                         <  2:5176117/146‑1 (MQ=255)
                                                                                                              cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                        <  2:186205/150‑1 (MQ=255)
                                                                                                                  cttttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                    <  1:4137790/148‑1 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCGACTCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATATCCTGATAGAAt                                  >  1:6844368/1‑150 (MQ=255)
                                                                                                                     ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTAAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                                 >  1:8426950/1‑150 (MQ=255)
                                                                                                                      tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                >  1:9116727/1‑150 (MQ=255)
                                                                                                                       ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                               >  2:8433988/1‑150 (MQ=255)
                                                                                                                       ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                               <  1:4561347/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:2425891/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:4483635/150‑1 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          <  1:7933439/150‑1 (MQ=255)
                                                                                                                              ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                        >  2:4858403/1‑150 (MQ=255)
                                                                                                                              ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                        >  2:8743695/1‑150 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  2:5000510/1‑150 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  2:2412288/1‑150 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  2:1425321/1‑150 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  1:979114/1‑150 (MQ=255)
                                                                                                                                ataAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      <  1:3438078/150‑1 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     >  2:1536726/1‑150 (MQ=255)
                                                                                                                                  aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                    >  2:4227465/1‑150 (MQ=255)
                                                                                                                                   aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                   <  2:2558336/150‑1 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  <  2:3750524/150‑1 (MQ=255)
                                                                                                                                      taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa                <  1:8272754/150‑1 (MQ=255)
                                                                                                                                        atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt              >  2:7667264/1‑150 (MQ=255)
                                                                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  1:1927945/150‑1 (MQ=255)
                                                                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  1:868947/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          >  2:9200601/1‑150 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          >  2:2979759/1‑150 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          <  2:2896072/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          >  1:3972943/1‑150 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCTCTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  2:7871583/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:5006368/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  1:3765749/1‑150 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:3518824/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:2607175/150‑1 (MQ=255)
                                                                                                                                               gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa       <  1:1421281/150‑1 (MQ=255)
                                                                                                                                                aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt      >  2:2006120/1‑150 (MQ=255)
                                                                                                                                                  aTCAAGACAATAGCGATGTAAAGCGCTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc    >  1:5457471/1‑150 (MQ=255)
                                                                                                                                                    cAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGTCGCGCACTATCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  2:6822683/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: