Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,832 G→T 6.4% intergenic (‑86/+237) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8320GT 6.4% 1615.6 / 21.8 479intergenic (‑86/+237)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base G (167/268);  new base T (41/3);  total (208/271)
Fisher's exact test for biased strand distribution p-value = 3.90e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 504 total aligned reads displayed.

AAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGA  >  NC_000913/2725683‑2725981
                                                                                                                                                     |                                                                                                                                                      
aaaTGCGGCGACGAGTATCACTACTTGTCAGTTTTCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATttgttg                                                                                                                                                        <  1:7877409/150‑1 (MQ=255)
aaaTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATttgttg                                                                                                                                                        <  1:5681235/150‑1 (MQ=255)
  aTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCa                                                                                                                                                      <  1:5818911/150‑1 (MQ=255)
   tGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAATGAAATATTATAAATTTGTTGAat                                                                                                                                                     >  2:8228902/1‑150 (MQ=255)
       gcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCa                                                                                                                                                 <  2:6989274/150‑1 (MQ=255)
       gcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCa                                                                                                                                                 <  2:5488208/150‑1 (MQ=255)
       gcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCa                                                                                                                                                 <  1:8414069/150‑1 (MQ=255)
         gacgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCAtt                                                                                                                                               <  1:223863/150‑1 (MQ=255)
                aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATAATGATATGCAAc                                                                                                                                       <  2:6209005/150‑1 (MQ=255)
                 tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                                                                                                                      >  2:469895/1‑150 (MQ=255)
                 tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                                                                                                                      >  2:7348263/1‑150 (MQ=255)
                   actactTGTCAGTTTGCTGTCTGCCGTTACAGTTCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTGTTAAGGTAATATTTTTAATTTGTTGGATTTAATTTTTCAAACtt                                                                                                                                    >  1:6268844/1‑150 (MQ=255)
                   actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATAATTATTCAAccat                                                                                                                                    >  2:2634146/1‑148 (MQ=255)
                     gccgtGTCAGTTTGCTGTCTGCCGGTACAGGACTTTCCGCCATGCCATGATGTCTCCTGCCGGTATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTaa                                                                                                                                  <  2:1176576/146‑1 (MQ=255)
                      actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                                 <  2:2450859/150‑1 (MQ=255)
                        ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATATTATACATTTTTTTAATATAATTATTCAACCTTaaaaa                                                                                                                               >  2:5723808/1‑147 (MQ=255)
                        ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCa                                                                                                                               <  1:1917139/150‑1 (MQ=255)
                           tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                            <  1:7458751/150‑1 (MQ=255)
                            cAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGGTGTTTATTTTTTTTTTAAATGAAATATTATAAATTTTTTTCATATCATTATGCAAACCTaaaaaagaa                                                                                                                           >  1:3684471/1‑143 (MQ=255)
                              gTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTGCATATAATTATTCAAACCTTAACAATAAAt                                                                                                                          >  1:154796/1‑150 (MQ=255)
                              gTTTGCTGTCTGCCGTTACAGTACTGTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAtt                                                                                                                         <  1:6706285/150‑1 (MQ=255)
                               tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTGTTGTCGGTCGCTGTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAAGAAttt                                                                                                                        >  2:5901577/1‑150 (MQ=255)
                                ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                       <  2:5175244/150‑1 (MQ=255)
                                  gCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagt                                                                                                                     <  2:1976157/150‑1 (MQ=255)
                                    tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTTAAGGAAATATTATAAATTTTTTTCAAATCATTATGAAAACATAAACAAGTATtttgttt                                                                                                                   >  2:6273815/1‑149 (MQ=255)
                                    tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAAAATTATAAATTTTTTTGATATAATTATTCAAACATAAACAATAATttagttt                                                                                                                   >  1:7199046/1‑149 (MQ=255)
                                       cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                                >  2:6821604/1‑150 (MQ=255)
                                       cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                                <  1:3468052/150‑1 (MQ=255)
                                        tGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTATTTTTTTTTTTAATTAAAAATTATAAAATTTTTTGATATAAATATGAAACCATAAACAAGAAAttagttttcag                                                                                                               >  1:2610849/1‑145 (MQ=255)
                                         gCCGTTACAGTACATTCACGCATGCCTTTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTAAAGGAAATATTATACATTTGTTGCACATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGa                                                                                                             <  2:6017992/150‑1 (MQ=255)
                                          ccGTTACAGTACTTTCAGCCATGCCTTTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTATTTTTTTTTTAAAGGAAATATTATACATTTGTTTCATATAATTATGACACCTTAACCAAGAATTTATTTAGCAGGa                                                                                                             >  2:933595/1‑150 (MQ=255)
                                            gTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGaaa                                                                                                           >  2:8061309/1‑150 (MQ=255)
                                               aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                                        <  1:4967910/150‑1 (MQ=255)
                                                cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                       <  1:7986834/150‑1 (MQ=255)
                                                 aGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGt                                                                                                      <  2:3334400/150‑1 (MQ=255)
                                                 aGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAATGAAATATTATAAATTTGTTGCATATCATTATGCAACCATAACCCTTAAGTTAGTTTGCAGGAAAATggg                                                                                                      >  1:7651217/1‑149 (MQ=255)
                                                  gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGtt                                                                                                     <  2:4962266/150‑1 (MQ=255)
                                                     cTTTCAGCCATGCCAGTCTGTCTCCTGCCTTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                  <  2:6643452/150‑1 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAAGGAAAAATTAAAAATTTGTTTCATATCAATATTCAAACATAAACAAGAAATTAGTTAGCAGGAAAATGGGTATTg                                                                                                 >  1:6937614/1‑150 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                 >  2:6117814/1‑150 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTTTTTTTTTTTTTAATGAAATATTATAAAATTTTTTGATTTAATTATTAAAACCAAACCAAGAAATTAGTTATAAGGAAAATGGTTTTTg                                                                                                 >  1:4384424/1‑150 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTCTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                 <  2:3065930/150‑1 (MQ=255)
                                                          aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCCTATCATTATGCAACCTTAACCATGAAATTAGTTAGCAGGAAAATTGTTATTgagga                                                                                             >  2:1730665/1‑150 (MQ=255)
                                                          aGCCATGCCATGATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGATATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagga                                                                                             <  2:7583629/150‑1 (MQ=255)
                                                           gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                                            >  2:7739183/1‑150 (MQ=255)
                                                            ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTTAGGAGc                                                                                           >  2:1284659/1‑150 (MQ=255)
                                                             catgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCt                                                                                          >  2:2327521/1‑150 (MQ=255)
                                                              atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTTTAAAGGAAATATTATACAATTGTTGCATATCAATATTCAACCATAAACAAGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCtt                                                                                         >  2:8875761/1‑150 (MQ=255)
                                                                  catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATTCAACCTTAACCATGAATTTAGTTAGCAGGAAAATTGTTATTGAGGAGCTTAAgg                                                                                     >  2:1767928/1‑150 (MQ=255)
                                                                   atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTGTTTTTTTTTTTTTTTAAGGAAAAAATATAAATTTTTTTGATAAAAATAATAAACCAAAAACAATAAATTATTTTGCAGGAAAATTTGTATTTAGGATCTTAATGa                                                                                    >  1:641137/1‑150 (MQ=255)
                                                                           tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTc                                                                            <  2:8557260/150‑1 (MQ=255)
                                                                            ccTGCCGTAATCCGCTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                           <  1:1694154/150‑1 (MQ=255)
                                                                            ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                           <  1:8105273/150‑1 (MQ=255)
                                                                             cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATAAATTTGTTGCATATCATTATTCAACCTTAACCAAGAATTTAGTTAGCAGGAAAATGGGTATTGAGGAGCTTAAGGATAAATTTCTg                                                                          >  2:2769949/1‑150 (MQ=255)
                                                                             cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                          <  2:7200159/150‑1 (MQ=255)
                                                                                ccGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTTTAAATGAAATATTATACAATTTTTTCATATAATTATTAAACCATAAACATGTATTTATTTATCAGGAAAATGGTTATTGAGGAGCTTAATGGTAAATTTATGGtt                                                                       >  2:5554147/1‑149 (MQ=255)
                                                                                 cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTaa                                                                      <  1:3251398/150‑1 (MQ=255)
                                                                                 cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTaa                                                                      <  1:9054658/150‑1 (MQ=255)
                                                                                    aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                                   <  2:3954485/150‑1 (MQ=255)
                                                                                    aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                                   <  2:3684471/150‑1 (MQ=255)
                                                                                      tCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAaggagg                                                                 <  2:2498996/150‑1 (MQ=255)
                                                                                         gATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTAAACATTTTTTGCAAATCATTATTCAACCTTAACCAAGAATTTAGTTAGCAGGAAAATGGTTAATGAGGAGCTTAAGGATAAATTTATGGTAAGGAGGaca                                                              >  2:833832/1‑150 (MQ=255)
                                                                                          aggCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGacac                                                             <  1:318038/148‑1 (MQ=255)
                                                                                             cTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTa                                                          <  2:439793/150‑1 (MQ=255)
                                                                                                ttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATgg                                                       <  1:5104222/150‑1 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTATGGGAAGGAGGACACGTATGGaa                                                     >  2:4600002/1‑150 (MQ=255)
                                                                                                        cgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGc                                               <  2:1050423/150‑1 (MQ=255)
                                                                                                          cTTTTGTTTATTTTTTTTGTAAAGTAAATATTATACATTTGTTGCATATCATTATGCAAACTTAACCATGAATTTATTTAGCATGAAATTGGTTATTGAGGAGCTTATGTATATATTTCTTGTAAGGAGGACACGTATGGAAGTGGGCaa                                             <  1:204475/150‑1 (MQ=255)
                                                                                                           ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCGTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                            >  1:4624840/1‑150 (MQ=255)
                                                                                                           ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                            >  1:5951863/1‑150 (MQ=255)
                                                                                                             ttGTTTATTTTTTTTGTAAAGGAATTATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCTAGtt                                          <  2:8702515/150‑1 (MQ=255)
                                                                                                             ttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGtt                                          <  2:3580673/150‑1 (MQ=255)
                                                                                                              tGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                         <  2:5789149/150‑1 (MQ=255)
                                                                                                                tttATTTTTTTTGTACAGGAAATATTATACAATTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                       <  1:9284719/150‑1 (MQ=255)
                                                                                                                tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                       <  2:4276737/150‑1 (MQ=255)
                                                                                                                tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                       >  1:708098/1‑150 (MQ=255)
                                                                                                                tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                       >  1:5479772/1‑150 (MQ=255)
                                                                                                                 ttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGACGTGGGCAAGTTgggg                                      >  1:4725267/1‑150 (MQ=255)
                                                                                                                   aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAGGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                                    >  2:9283614/1‑150 (MQ=255)
                                                                                                                   aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                                    <  2:2057158/150‑1 (MQ=255)
                                                                                                                    ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                   <  1:7348263/150‑1 (MQ=255)
                                                                                                                     tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                  >  1:4802229/1‑150 (MQ=255)
                                                                                                                     tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                  >  2:322186/1‑150 (MQ=255)
                                                                                                                       tttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCg                                >  2:5202685/1‑150 (MQ=255)
                                                                                                                        ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTTGGGAAGCCGt                               >  1:1105631/1‑150 (MQ=255)
                                                                                                                        ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGt                               <  1:8846543/150‑1 (MQ=255)
                                                                                                                           tGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGACGTGGGCAAGTTGGGGAAGCCGTATc                            >  2:3889884/1‑150 (MQ=255)
                                                                                                                            gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                           <  1:1354694/150‑1 (MQ=255)
                                                                                                                               aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt                        <  2:8989997/150‑1 (MQ=255)
                                                                                                                                aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                       <  1:3867535/150‑1 (MQ=255)
                                                                                                                                  gAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGGGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCt                     <  2:1561984/150‑1 (MQ=255)
                                                                                                                                  gAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCt                     >  2:7551817/1‑150 (MQ=255)
                                                                                                                                        ttatACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCt               >  1:3922034/1‑150 (MQ=255)
                                                                                                                                        ttatACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCt               >  1:4325653/1‑150 (MQ=255)
                                                                                                                                         tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg              <  1:4156453/150‑1 (MQ=255)
                                                                                                                                          ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg             <  2:7068317/150‑1 (MQ=255)
                                                                                                                                          ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg             >  2:3990273/1‑150 (MQ=255)
                                                                                                                                          ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg             <  1:1301109/150‑1 (MQ=255)
                                                                                                                                           taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCCAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGc            >  1:8561598/1‑150 (MQ=255)
                                                                                                                                                     gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa  <  1:5293806/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGA  >  NC_000913/2725683‑2725981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: