Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,609 T→G 8.4% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6090TG 8.4% 1093.3 / 43.6 378intergenic (+64/‑335)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (233/90);  new base G (9/20);  total (244/134)
Fisher's exact test for biased strand distribution p-value = 2.53e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 389 total aligned reads displayed.

GGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATT  >  NC_000913/3239461‑3239758
                                                                                                                                                     |                                                                                                                                                     
   cGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                                                                                                                                                   <  2:4535680/150‑1 (MQ=255)
       gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgag                                                                                                                                               >  1:7413281/1‑150 (MQ=255)
         gtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGagag                                                                                                                                             >  1:8471377/1‑150 (MQ=255)
         gtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGagag                                                                                                                                             <  1:59627/150‑1 (MQ=255)
         gtTTGGCATTCTGGTGATGACGTTTAGTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGagag                                                                                                                                             <  2:5363426/150‑1 (MQ=255)
          tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                            <  2:6118669/150‑1 (MQ=255)
          tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                            <  2:2449173/150‑1 (MQ=255)
          tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                            <  2:7895197/150‑1 (MQ=255)
               cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                       >  2:7230185/1‑150 (MQ=255)
                  tcagttgatgaCGTTTATTATCAACGCCTGTGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATATTTAATATGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACAGTTTTCTCCCTCTCCCTTCAAGGGAGAGGGTCGGGGt                                                                                                                                    >  1:8275684/6‑150 (MQ=255)
                  tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                    >  1:8679337/1‑150 (MQ=255)
                    tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                  >  1:235351/1‑150 (MQ=255)
                    tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                  >  1:5222105/1‑150 (MQ=255)
                     ggTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAg                                                                                                                                 >  2:3867718/1‑150 (MQ=255)
                      gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                                <  2:4337783/150‑1 (MQ=255)
                      gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                                <  2:1894090/150‑1 (MQ=255)
                          tgaCGTTTATTATCAACGCCTGGTTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTaa                                                                                                                            <  2:2100284/150‑1 (MQ=255)
                           gaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                                                                                           >  1:3205539/1‑150 (MQ=255)
                             cGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTGTTCTCCCTCTCCCTTCCAGGGCGAGGGTCGGGGTGAGGGTAAttt                                                                                                                         >  2:7433560/1‑150 (MQ=255)
                              gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                        >  2:6532433/1‑150 (MQ=255)
                               tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                       >  1:7830035/1‑150 (MQ=255)
                                 tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTTAGGGTAATTTTTCg                                                                                                                     <  2:4076547/150‑1 (MQ=255)
                                  attatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGc                                                                                                                    >  1:1401569/1‑150 (MQ=255)
                                   ttatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCa                                                                                                                   >  2:2235360/1‑150 (MQ=255)
                                     atCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                                                                                                 >  1:8210213/1‑150 (MQ=255)
                                      tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                                >  2:7754968/1‑150 (MQ=255)
                                         aCGCCTGGGTGAATTATCGGCATGATAAGCCGCGGGGGGGATAATTTTTAATCTGCCTCAGCCGGGTCCCCTGTCATTAACATGAGCACCGTGTTCTCCCTCTCCCTTCCAGGGAGAGGGGCGGGGTGAGGGTAATTGTTCGCACCGATg                                                                                                             >  2:8993037/1‑150 (MQ=255)
                                                gtttaaTTTTCGGCTTTTTAAGCTGGGGGTTGTTTATTTTTTAATCTGCCTAAGCGGTGTACCCTTTAATTAACATGAGCACGTTTTTCCCCCTCCCCCTCCCGGGGAGCGGGTGGGGTTGTGGGAAATTTTTCGCACCGATGCTGGCCt                                                                                                      <  2:551021/146‑1 (MQ=255)
                                                    atttATCGGCAGGATAAGCTGGGGGGTGTATATTTTTTAATCTGCCTAAGCGGTGTACCCTGTAATTAAAATGAGCACGTTTTTCTCCCTCCCCATCCCCGGGTGTGGGTCGGGGTTCGGTTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  <  2:3590319/148‑1 (MQ=255)
                                                    aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGTCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  >  1:3678055/1‑150 (MQ=255)
                                                    aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  >  1:157085/1‑150 (MQ=255)
                                                    aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  >  2:8466392/1‑150 (MQ=255)
                                                    aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTACCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  <  2:5726087/150‑1 (MQ=255)
                                                    aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGCCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCTCCGATGGTGGCCTGTTc                                                                                                  >  2:8194950/1‑150 (MQ=255)
                                                      ttATCGGCATGATAAGCAGGGGGGTGTATAATTTTTAATCGGCCTACGCCGTGTACCCGGTCATTAACATGAGCACGGTTTTCTCCCTCGCCCTTCCCGGGAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                                <  2:5181221/150‑1 (MQ=255)
                                                         tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTc                                                                                             >  2:6406454/1‑150 (MQ=255)
                                                                gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCTCCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                      >  1:2331043/1‑150 (MQ=255)
                                                                   aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCTAAccc                                                                                   >  1:4268325/1‑150 (MQ=255)
                                                                     gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctc                                                                                 >  2:6022040/1‑150 (MQ=255)
                                                                      cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                                >  1:8580057/1‑150 (MQ=255)
                                                                          ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                            >  2:4617949/1‑150 (MQ=255)
                                                                               gtatatTTTTTAATCTGCCTACGCGGTTTACCCTGTCTTTAACATGAGCACCTTTTTCCCCCTCCCCCTTCCGGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCGccccgtttg                                                                       <  1:2081018/148‑6 (MQ=21)
                                                                                gATAATTTTTAATCTGCCTAAGCGGTGTACCCTGTCATTAACATGAGCACCGTTTCCTCCCTCTCCCTGCCAGGGTTAGGGTCGGGTTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                      <  2:1261007/150‑1 (MQ=37)
                                                                                gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCAAACCCTCTCCCCAAACgg                                                                      >  1:7109838/1‑150 (MQ=255)
                                                                                  tattttttATTGTGCCTACGGTGTTTACCCTTTCATTAAATTTAGCACGTTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTGGGGTTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                    <  2:8088847/147‑1 (MQ=16)
                                                                                  tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                    >  2:588231/1‑150 (MQ=255)
                                                                                   attttttAATCGCCCTCCGCGGTGTACACTTTCTTTAAATTCCGCACCTTTGTCTCCCACACCGTGCCCGGGTGTGTGTGGGGGTCCGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                   <  1:5525778/148‑1 (MQ=12)
                                                                                     tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGGCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCgc                                                                 >  2:8791040/1‑149 (MQ=255)
                                                                                      ttttATTCTGCCTACGCCGTGTACCGTGCAATTAACATGAGCACCGTTTTCCCCCTCTCCCTTCCCGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                <  2:5698053/150‑1 (MQ=25)
                                                                                       tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                               >  1:754689/1‑150 (MQ=255)
                                                                                       tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGGTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                               >  2:3127334/1‑150 (MQ=255)
                                                                                            tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACt                                                          >  1:9038212/1‑150 (MQ=255)
                                                                                              tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                        >  1:947356/1‑150 (MQ=255)
                                                                                                ccTACGCCGTGTCCCCTGACTTAAACAGGCCCACCGTTTACCCCCCCGCCCTTCCTGGGTGTGGGTGGGGGTGTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                      <  1:4627613/150‑1 (MQ=18)
                                                                                                 cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                     >  1:799822/1‑150 (MQ=255)
                                                                                                 cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                     >  1:1071649/1‑150 (MQ=255)
                                                                                                  tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                    >  2:9263440/1‑150 (MQ=255)
                                                                                                   acgCCGTGTACCCTGTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                   <  1:2364079/148‑1 (MQ=255)
                                                                                                           tACCCTGAATTTAAAATGAGCACCGTTTTCCCCCTCCCCCTTCCAGGGAGAGGGTCGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                           <  1:3867718/150‑1 (MQ=21)
                                                                                                             cccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                         >  1:9187525/1‑150 (MQ=255)
                                                                                                              ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                        >  2:6933764/1‑150 (MQ=255)
                                                                                                              ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                        >  1:7242267/1‑150 (MQ=255)
                                                                                                              ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGa                                        >  1:8105216/1‑150 (MQ=255)
                                                                                                               gggTCATTAAAATGTGCACCGTTTTCTCCCTCTCCGTTCCTGGGCGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                       <  2:3061740/148‑1 (MQ=25)
                                                                                                               cTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                       >  1:6753300/1‑150 (MQ=255)
                                                                                                                tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                      >  2:1810434/1‑150 (MQ=255)
                                                                                                                    aTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                  >  1:4402157/1‑150 (MQ=255)
                                                                                                                    aTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                  >  2:4894054/1‑150 (MQ=255)
                                                                                                                       aaCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                               >  1:3364050/1‑150 (MQ=255)
                                                                                                                         cATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatca                             >  2:6952033/1‑150 (MQ=255)
                                                                                                                           ggcgCACGGTTTGCTCCCTCCCCCTGCCAGGGTGTGGTTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                           <  2:5112758/147‑1 (MQ=25)
                                                                                                                            gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                          >  1:6894196/1‑150 (MQ=255)
                                                                                                                             aGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                         <  1:7001235/150‑1 (MQ=255)
                                                                                                                              ccacgttttcCTCCCTCCCCCTCCCCGGGTGTGGGGGGGGGTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                        <  1:196087/144‑1 (MQ=25)
                                                                                                                              ccACCTTTTACACCCACTCCATACCAGGTATAGGGTCGGGTTTAGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                        <  2:8018180/149‑1 (MQ=25)
                                                                                                                               cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGGTCCCCTCACCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCCAGTCCTGGTTTGTTGTTGTCATCAGTCggg                       >  2:8611547/1‑150 (MQ=255)
                                                                                                                                cgggTTTTCCCCCTCTCCCTCCCGGGGTGTGGGGGGGGTTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                      <  1:1033759/147‑1 (MQ=25)
                                                                                                                                aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                      >  2:6762685/1‑150 (MQ=255)
                                                                                                                                aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTTTCATCAGTCTGGa                      >  1:6359118/1‑150 (MQ=255)
                                                                                                                                aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCTAACCCTCTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTGTCATAAGTCTGGa                      >  1:4116829/1‑150 (MQ=255)
                                                                                                                                  cGTTTTCCCCCCTCCCCCTCCGCGGGTGTGGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                     <  2:1675422/150‑1 (MQ=25)
                                                                                                                                   gTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                   <  2:8570608/150‑1 (MQ=255)
                                                                                                                                    ttttCCCCCTCCCCCTCCCTGGGTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGcc                  <  1:8392818/150‑1 (MQ=25)
                                                                                                                                        ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc              >  1:2199502/1‑150 (MQ=255)
                                                                                                                                            cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt           >  2:3707061/1‑150 (MQ=255)
                                                                                                                                             ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg          >  2:1549104/1‑150 (MQ=255)
                                                                                                                                             ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg          >  1:1832510/1‑150 (MQ=255)
                                                                                                                                             ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg          >  1:5639637/1‑150 (MQ=255)
                                                                                                                                             ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCCCCCTAACCCACTCCCCAAACGGGGCGCGGGGCCTGACCGAGTCCTTTTTTGATGTTGTCCTCAGTCTGGAAGCCGCACGTTg          >  1:2501348/1‑150 (MQ=255)
                                                                                                                                             ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTAACCTCACCCTAACCCACACCCCAAACGGGGCGAGGGGACGGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGTAAGCCGCACGttt          >  1:299847/1‑149 (MQ=255)
                                                                                                                                             ctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg          >  1:261527/1‑150 (MQ=255)
                                                                                                                                              cctccctTCCGGGGTGAGGGTCGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg         <  2:252451/149‑1 (MQ=255)
                                                                                                                                                tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCCCGTTGGCt       >  2:4826510/1‑150 (MQ=255)
                                                                                                                                                tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGGTGGCt       >  1:6282609/1‑150 (MQ=255)
                                                                                                                                                  cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt     >  2:9117454/1‑150 (MQ=255)
                                                                                                                                                  cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt     >  2:266171/1‑150 (MQ=255)
                                                                                                                                                   ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa    >  1:5114040/1‑150 (MQ=255)
                                                                                                                                                    ttCCGGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGACTTTAt   <  2:1144120/150‑1 (MQ=37)
                                                                                                                                                    ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt   >  2:5012983/1‑150 (MQ=255)
                                                                                                                                                    ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt   >  2:3375759/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATT  >  NC_000913/3239461‑3239758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: