Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,518 T→A 6.8% N48I (AAT→ATT)  tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5180TA 6.8% 1541.0 / 8.5 509N48I (AAT→ATT) tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base T (162/272);  new base A (45/2);  total (235/274)
Fisher's exact test for biased strand distribution p-value = 6.94e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 515 total aligned reads displayed.

CGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTT  >  NC_000913/3263369‑3263667
                                                                                                                                                     |                                                                                                                                                     
    cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAc                                                                                                                                                   <  1:2411569/150‑1 (MQ=255)
    cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAc                                                                                                                                                   <  1:302033/150‑1 (MQ=255)
    cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAc                                                                                                                                                   <  1:340584/150‑1 (MQ=255)
    cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGGGCAGGCTCTCCCCCAATTAc                                                                                                                                                   >  2:6428250/1‑150 (MQ=255)
        gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGAt                                                                                                                                               >  1:9145071/1‑150 (MQ=255)
         tAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGTGCCCGCGGTGCTGGCTCGCCCCCAATTAAGGTTa                                                                                                                                              >  2:4164897/1‑150 (MQ=255)
             aaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                                                          <  2:7464624/150‑1 (MQ=255)
                 cTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCa                                                                                                                                      <  2:4282805/150‑1 (MQ=255)
                  tGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAt                                                                                                                                     >  2:7669433/1‑150 (MQ=255)
                    ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCGCGGGGCGGGGTGGCCCCCCGTTAAGGATAAGAATGCAgtt                                                                                                                                   >  1:5542412/1‑150 (MQ=255)
                    ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAttt                                                                                                                                   >  2:6997024/1‑150 (MQ=255)
                    ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAATTAAGGATAAGAATGCAttt                                                                                                                                   >  1:5913075/1‑150 (MQ=255)
                      gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCGCTCCCCCCGTTACGGATAAGAAAGCCATTTc                                                                                                                                 >  2:3488168/1‑150 (MQ=255)
                        cgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCg                                                                                                                               <  1:8278263/150‑1 (MQ=255)
                         gTGAGCGATGCGGTGGCCAATTCAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                                              <  2:8933946/150‑1 (MQ=255)
                         gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAACTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                                              <  1:2234712/150‑1 (MQ=255)
                         gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                                              <  2:6150431/150‑1 (MQ=255)
                         gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                                              <  2:8192954/150‑1 (MQ=255)
                            aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGtt                                                                                                                           >  2:5268851/1‑150 (MQ=255)
                                 tGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGGGCGGGCGCTCCCCCCATTACGGATAAGAATGCAATTTACGAGGTAATAc                                                                                                                      >  1:3889621/1‑150 (MQ=255)
                                 tGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAc                                                                                                                      <  2:2245435/150‑1 (MQ=255)
                                 tGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAc                                                                                                                      <  1:5259355/150‑1 (MQ=255)
                                  gCGGTGGACAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAcc                                                                                                                     <  1:9000191/150‑1 (MQ=255)
                                     gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCGCGGGGCGGGCGCGCCCCCCGTTACGGCTCAGAATGCAATTTCCGAGTTAATACCCTc                                                                                                                  >  2:7247475/1‑150 (MQ=255)
                                     gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTc                                                                                                                  <  2:8858947/150‑1 (MQ=255)
                                      tGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGTTGCAGCGGGGCTGGCTCTGCCCCCAGTAAGGATAAGAATGGATTTTAAGAGTTAATAACGTCg                                                                                                                 >  2:7827826/1‑150 (MQ=255)
                                       ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                                <  2:2862391/150‑1 (MQ=255)
                                        gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCACCGGGGCGGGCGCGCCCCCCCTTACCGCGAAGCATGCAATTTCCGCGTTAAAACCCGCGGc                                                                                                               >  2:2545141/1‑150 (MQ=255)
                                        gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCCCGGGGCTGGCGCGCCCCCCAATACGGATAAGAATTGATTTTACGAGTTAAAAACggcggc                                                                                                               >  1:334825/1‑150 (MQ=255)
                                             ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAc                                                                                                          >  2:2951766/1‑150 (MQ=255)
                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                                      <  2:707666/150‑1 (MQ=255)
                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                                      <  1:4727712/150‑1 (MQ=255)
                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                                      <  1:6706840/150‑1 (MQ=255)
                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                                      <  1:3731199/150‑1 (MQ=255)
                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                                      <  1:1871112/150‑1 (MQ=255)
                                                       acTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTaa                                                                                                <  1:8917336/150‑1 (MQ=255)
                                                         tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                                              <  2:2011672/150‑1 (MQ=255)
                                                         tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                                              <  1:4341389/150‑1 (MQ=255)
                                                            cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACtt                                                                                           <  1:7321214/150‑1 (MQ=255)
                                                            cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACtt                                                                                           <  1:4487906/150‑1 (MQ=255)
                                                            cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACtt                                                                                           <  2:9145071/150‑1 (MQ=255)
                                                              tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAGCGGTGCTGGCTGTCCCCCATTTAAGGGTAAGAATGAATTTTCCGCGTTAAAAACGGCGGGCAAACCAGAAAGTAATaaataa                                                                                         >  1:6270683/1‑145 (MQ=255)
                                                              tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTca                                                                                         <  2:2399677/150‑1 (MQ=255)
                                                                tAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcaca                                                                                       <  2:6091548/150‑1 (MQ=255)
                                                                tAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcaca                                                                                       <  1:1005256/150‑1 (MQ=255)
                                                                 aaaTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCCCGGTGCTGGGTCTCCCCCAGTTAAGGGTAAGAATGCATTTTACGAGGTAATAACCTCGGGCAAACCAGACAATAATAcataaaag                                                                                      >  1:1269335/1‑143 (MQ=255)
                                                                 aaaTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACGGCTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAg                                                                                      >  2:7585678/1‑150 (MQ=255)
                                                                   aTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTc                                                                                    <  2:6684295/150‑1 (MQ=255)
                                                                    ttCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATGCATTTTCCGAGTTAATAACGTCGGCAATAACTGACATTAATACTTCAAAGTCg                                                                                   >  2:1324808/1‑150 (MQ=255)
                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                                <  1:3960221/150‑1 (MQ=255)
                                                                        gattttCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                                               <  2:3277695/148‑1 (MQ=255)
                                                                        gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATTCATTTTCCGAGTTAATACCGTCGGCAATAACAGACAATAAAAATTCAAAGTCGCggg                                                                               >  2:8014224/1‑150 (MQ=255)
                                                                        gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTGCTGGCTCTCCCCCAGTTACGGATAAGAAAGCATTTTCCGAGTTAATAACCTCGGCAAAAACAGACATTAATAATTCACAGTCGcgtg                                                                               >  2:4819297/1‑147 (MQ=255)
                                                                             tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTGCAGGCTCGCCCCCAATTACCGATAAGAATGCAATTTCCCAGGTAAAACCCTCGGCAAAACCAGACAATAATACATCACAGTCGCGGGccacg                                                                          >  2:4419255/1‑146 (MQ=255)
                                                                               cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAg                                                                        <  1:2017881/150‑1 (MQ=255)
                                                                                aGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAATTTCCGAGGTAATACCGTCGGCAATACCAGACATTAATACTTCACAGTCGCTGGCATCGAGc                                                                       >  2:548354/1‑150 (MQ=255)
                                                                                 gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCa                                                                      >  2:3915221/1‑150 (MQ=255)
                                                                                 gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCa                                                                      <  1:5375171/150‑1 (MQ=255)
                                                                                        tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaaa                                                               <  1:7611520/150‑1 (MQ=255)
                                                                                        tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaaa                                                               <  2:1610219/150‑1 (MQ=255)
                                                                                        tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaaa                                                               <  1:2948680/150‑1 (MQ=255)
                                                                                          cAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCACCGGTGGTGGCTCTCCCCCAATTTAGGATAAGAATGCAATTTACGAGTTTATACCGTCGGCAATAACAGACATTAATAATTAACAGTAGCGGGGCTAGGGCACGGGAAAca                                                             >  2:3409405/1‑149 (MQ=255)
                                                                                              tGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAAt                                                         <  1:7048500/150‑1 (MQ=255)
                                                                                                  ttCAATGCACCTTCGTAGCTGTGGTGTGCCAGCGGTTCTGGCTCTCCCCCAGTTAAGGATAAGAAAGCATTTTACGAGTTAATAACGGCGGCCAAACCGGACATTAATAATTAAAAGTCGCGGGCCTAGAGCACCGGAAACATAATCGaa                                                     >  2:850497/1‑150 (MQ=255)
                                                                                                  ttCAATGCACCTTCGTAGCTGTGGTGTGCCAGCGGTGCTGGCTCTCCCCCAATTAAGGATAAGAAAGCAATTTACGAGTTAATAACCTCGGCAAAAACAGAAAATAAAAAATAAAAGTCGCTGGCAACGAGCACCGAAAAATTAATAGaa                                                     >  2:1022373/1‑150 (MQ=255)
                                                                                                  ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGaa                                                     <  2:1107035/150‑1 (MQ=255)
                                                                                                    cAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                   <  2:3148461/150‑1 (MQ=255)
                                                                                                      aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAGTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg                                                 >  2:7751059/1‑150 (MQ=255)
                                                                                                        gCACCTTCGTAGCTGTGGTGGGCCCGCGGTGCGGGCTCTCCCCCATTTACCGGAAAGAAATCAATTTACCAGTTAAAACCCTCGGCAAAAACAGACAATAAAAATTAAAACTAGCGGGCAGAGCGCACGGGAAAAATAATAGaaatacca                                               >  2:8592200/1‑144 (MQ=255)
                                                                                                        gCACCTTCGTAGCTGTGGTGGGCCAACGGTGCTGGCTCACCCCCAGTTAAGCATAAAAAAGCATATTACGACGTAATACCCTCGCCAAAACCACACAATAATACGTCACCGGCGCGGGCCACGAGCCCCGAAAACATAAACGGAGACCaa                                               >  2:7153802/1‑150 (MQ=255)
                                                                                                        gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa                                               <  2:4549679/150‑1 (MQ=255)
                                                                                                            cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAACGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCCTCGAGCACGGACAACATAATCGAAGACGAACcac                                           >  2:5781226/1‑150 (MQ=255)
                                                                                                             ttCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcaca                                          <  2:5724599/150‑1 (MQ=255)
                                                                                                               cGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGt                                        <  2:40244/150‑1 (MQ=255)
                                                                                                                   gCTGTGGTGGGCCAGCGGGGCTGGCTCTCCCCCAATTACGGATAAGAATGCATTTTACGAGTTAATAACGTCGGCAAAACCAGACAAAAAAAAATAACAGTCGCTGGCAACGAGCACGGAAAAATAAATAGAAGAAGAAACACACTtaaa                                    >  2:5136428/1‑149 (MQ=255)
                                                                                                                     tgtgGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaataa                                  <  2:564444/150‑1 (MQ=255)
                                                                                                                       tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAATAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc                                <  1:7827826/150‑1 (MQ=255)
                                                                                                                         gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa                              >  2:1768588/1‑150 (MQ=255)
                                                                                                                         gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa                              <  2:4976891/150‑1 (MQ=255)
                                                                                                                         gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa                              <  2:4709961/150‑1 (MQ=255)
                                                                                                                            aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac                           <  1:4162134/150‑1 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                   >  2:3745727/1‑124 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaa                         >  1:8436381/1‑150 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaa                         >  2:8360189/1‑150 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaa                         >  2:8053713/1‑150 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaa                         >  1:4858168/1‑150 (MQ=255)
                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaa                         >  1:6386458/1‑150 (MQ=255)
                                                                                                                               cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaac                        >  2:1223458/1‑150 (MQ=255)
                                                                                                                                  cGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCTAAGACGAACAACAGTTAATAACCAAAACAACCgg                     >  1:7228348/1‑150 (MQ=255)
                                                                                                                                  cGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCgg                     <  2:2094499/150‑1 (MQ=255)
                                                                                                                                    gTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAGACAACCGGaa                   >  2:2206179/1‑150 (MQ=255)
                                                                                                                                    gTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaa                   >  2:7298914/1‑150 (MQ=255)
                                                                                                                                     tggtGGCTCTCCCCCATTTAGGTATATTATTGCTTTTTCCGTTTTAATAGCGTCGGAAATACCTGACATTAATACTTCACTGTCGGTGGCATCGAGCTCGTAAAACTTAATCTAAGACGAACCAATGTTAATAACAAAAACAACCGGaaa                  <  1:8040329/147‑1 (MQ=255)
                                                                                                                                        tGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattc               <  1:5666658/150‑1 (MQ=255)
                                                                                                                                         ggCTCTCCCCAATTTCCGGATAATAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAAATTAATACTTCACAGTCGCTGGCATCGAGCACGTAAAACTTAATCGAAGACGAACCACATTTAATAACCAAAACAACCGGAAattca              <  1:2109838/150‑1 (MQ=255)
                                                                                                                                           ctctCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt            <  2:1440203/150‑1 (MQ=255)
                                                                                                                                              tcCCCCATTTACGGATAAGAATGAATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATtcattcat         <  1:9271489/150‑1 (MQ=255)
                                                                                                                                                ccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtct       >  1:2894182/1‑150 (MQ=255)
                                                                                                                                                 cccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc      >  1:8822677/1‑150 (MQ=255)
                                                                                                                                                  ccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATctct     >  2:3065680/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTT  >  NC_000913/3263369‑3263667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: