Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,328 A→C 5.2% intergenic (‑300/+130) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,3280AC 5.2% 1791.9 / 13.2 611intergenic (‑300/+130)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (383/167);  new base C (2/44);  total (391/220)
Fisher's exact test for biased strand distribution p-value = 3.00e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 637 total aligned reads displayed.

CGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAC  >  NC_000913/3392179‑3392477
                                                                                                                                                     |                                                                                                                                                     
     aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                                  <  1:3903773/150‑1 (MQ=255)
      cGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                                                                                 >  1:3053604/1‑150 (MQ=255)
      cGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                                                                                 <  2:7765288/150‑1 (MQ=255)
       gCCTTATCCGACCTACGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                                                <  2:9288343/150‑1 (MQ=255)
       gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                                                >  1:5289791/1‑150 (MQ=255)
       gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                                                >  1:8823151/1‑150 (MQ=255)
        ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTCAACGCCATATCCGACCTACGGTTATGTTCAGGATCTATGCCGGATAAGACTCACACCGTCgaa                                                                                                                                               >  2:1367844/1‑148 (MQ=11)
        ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                                                                               >  2:5765601/1‑150 (MQ=255)
         cTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                                                                              <  1:4934906/150‑1 (MQ=255)
         cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                                                                              >  2:4753707/1‑150 (MQ=255)
            aTCCGACTTTCGTTTTTTTTGGGTTTTTCGGCCTGATAAGAGGCACCGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTTTGTTCCGTTTGTTGGGCTGATGCGACGCTGTGCGTCTTATCAg                                                                                                                                           <  2:7070987/150‑1 (MQ=9)
             tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                                          >  1:2046074/1‑150 (MQ=40)
             cccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAgg                                                                                                                                          <  2:4938706/149‑1 (MQ=14)
                 aCCTACGTTTTTTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                                                                                                      <  2:2010499/150‑1 (MQ=255)
                 aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                                                                                                      >  2:6384824/1‑150 (MQ=37)
                    tACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                                                                                                   >  2:3747994/1‑150 (MQ=35)
                         ttATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCg                                                                                                                              >  1:1892603/1‑150 (MQ=35)
                           ttGTTCGTTTTGTAGGCCTTATAAGACGCACCGCGTCGCAACCGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTTTGTTCGTTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa                                                                                                                            <  2:2802845/149‑1 (MQ=11)
                           ttGTTCCGTTTGTGGGCCTGCTACGAGGCCCCGCGGCGCATCTGGCAAGGGGTGCGGGATGCGGGGTAAACGCCTTATCCGACCTCGGGTTATGTTCCGTTTGTCGGCCTGATACGCCGCCCCGGGTCGCATCAGGCAACGGCTGCCGGa                                                                                                                            <  2:4183935/149‑1 (MQ=2)
                              ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                                                                         >  1:9347057/1‑150 (MQ=35)
                              ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGAGACGCATCAGGCAACGGCTGACGGAAgc                                                                                                                         >  2:9170499/1‑150 (MQ=11)
                               tCCTTTTTTAGGCATTACAAGACGCACCCCGGCGCATCCGCCAACGGCTGCCGGATGCGGCGTAACCGCCTAATCCGACCTACGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                                                                        <  2:1075381/150‑1 (MQ=11)
                               tCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATCAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAGgcg                                                                                                                        >  1:8955544/1‑150 (MQ=255)
                                  gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                                                                     >  1:4653369/1‑150 (MQ=32)
                                          gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGCGTGAACGcc                                                                                                             >  2:5942199/1‑150 (MQ=21)
                                             tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTa                                                                                                          >  1:5873492/1‑150 (MQ=37)
                                               aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATc                                                                                                        >  2:7644613/1‑150 (MQ=37)
                                                tAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATcc                                                                                                       >  1:6357424/1‑150 (MQ=37)
                                                  aGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGa                                                                                                     >  1:5439160/1‑150 (MQ=37)
                                                   gACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGt                                                                                                                                                   <  1:4216099/103‑1 (MQ=32)
                                                      gCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTAGCCGACCTa                                                                                                 >  2:8414544/1‑150 (MQ=255)
                                                        cccgCGTCGCTTCTGGCACCGGGGGCCGGAGGCGGGGTAACCGCCTTATCCGACCTCGGGTTTTGTTCCGTTTGTTGGGCTGTTAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACg                                                                                               <  2:1224545/147‑1 (MQ=11)
                                                        acaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACg                                                                                               >  2:5641852/1‑150 (MQ=37)
                                                        acaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACg                                                                                               >  1:7615671/1‑150 (MQ=37)
                                                        acaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAGGCGGCGAGAACGCCCTCGCCGACCTACg                                                                                               >  2:1593018/1‑150 (MQ=255)
                                                        acaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCCTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACg                                                                                               >  1:1563092/1‑150 (MQ=37)
                                                                  aTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCTCAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCt                                                                                     >  1:574524/1‑150 (MQ=38)
                                                                      ggCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCt                                                                                 >  2:322376/1‑150 (MQ=40)
                                                                       gccaCGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTc                                                                                >  1:7782099/4‑150 (MQ=255)
                                                                        cAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCg                                                                               >  1:8401174/1‑150 (MQ=255)
                                                                          aCGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGtt                                                                             <  2:8057554/150‑1 (MQ=255)
                                                                            ggCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTgg                                                                           >  2:8649286/1‑150 (MQ=255)
                                                                              cTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGcc                                                                         <  2:1531421/150‑1 (MQ=255)
                                                                                  cgtaTGCGGGGTAAACGCGTTACCCGACCTCCGTTTTTTTGCCTTTTGTAGGCCTGTTAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  1:7992871/147‑1 (MQ=17)
                                                                                  cgtaTGCGGCGTAAAGGCGTTACCGGACCTACGTTTATGTCCCTTTTGTAGGCCTGTTTAGACCCACCGGGTCTCTTCTGGCTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  1:5095749/147‑1 (MQ=17)
                                                                                  cGGTTGCGGGGTAACCGCCTTAGCGGACCTAGGGTTTGGTGCGGTTTGTAGGCCTGATACGACGCCCCGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  1:6010971/150‑1 (MQ=17)
                                                                                  cGGATGGGGCGTAACCGCCTTATCCGACCTCCGGTTTTGTTCCGTTTGTCGGCATTATACTCCGCACAGCGTCGCATCTGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  1:8198505/150‑1 (MQ=17)
                                                                                  cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  2:5405485/150‑1 (MQ=255)
                                                                                  cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTCATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     >  1:440217/1‑150 (MQ=255)
                                                                                    gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGc                                                                   >  2:4019409/1‑150 (MQ=255)
                                                                                        cggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGGTGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACg                                                               >  1:3640171/1‑150 (MQ=255)
                                                                                         ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGa                                                              >  1:2851504/1‑150 (MQ=255)
                                                                                         ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGa                                                              >  2:732980/1‑150 (MQ=255)
                                                                                                  gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTAGGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTAt                                                     >  1:9025036/1‑150 (MQ=255)
                                                                                                   ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATg                                                    <  1:6459133/150‑1 (MQ=255)
                                                                                                      tATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATgaga                                                 >  1:6713497/1‑150 (MQ=255)
                                                                                                       aTCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGAt                                                <  1:1170480/150‑1 (MQ=255)
                                                                                                        tCCTACCTACGTTTATGTTCCGATTTTAGGACTGATAAGTTGCAGCCCGTCGCTTATTTCTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGAtt                                               <  2:1689571/150‑1 (MQ=18)
                                                                                                        tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGAtt                                               >  2:5203724/1‑150 (MQ=255)
                                                                                                        tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGAtt                                               <  2:9021432/150‑1 (MQ=255)
                                                                                                         ccGACCTACGGTTTGGGTCGGTTTGTAGGCCTTATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                              <  1:2244328/150‑1 (MQ=21)
                                                                                                         ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                              <  2:6952509/150‑1 (MQ=255)
                                                                                                         ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                              <  2:8820685/150‑1 (MQ=255)
                                                                                                           gACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTggg                                            <  2:5821745/150‑1 (MQ=255)
                                                                                                            aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                           >  1:6942028/1‑150 (MQ=255)
                                                                                                                aCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAAt                                       >  1:4889112/1‑150 (MQ=255)
                                                                                                                  ggTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTa                                     >  1:1746389/1‑150 (MQ=255)
                                                                                                                  ggTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTa                                     >  2:71856/1‑150 (MQ=255)
                                                                                                                  ggTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTAAGTTCAGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTa                                     >  1:3594633/1‑150 (MQ=255)
                                                                                                                   gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAc                                    <  2:7827166/150‑1 (MQ=255)
                                                                                                                   gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAc                                    <  1:3281926/150‑1 (MQ=255)
                                                                                                                    ttATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcg                                   >  1:270312/1‑150 (MQ=255)
                                                                                                                     tATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcgc                                  <  2:3052430/150‑1 (MQ=255)
                                                                                                                          tCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCt                             >  2:8247337/1‑150 (MQ=255)
                                                                                                                           ccttttGCAGGCCTGCAAAGACGCACCGCGCCGCATCCGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTCCGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTCAGAGATTGGGGCAATTACGCGCCCTc                            <  1:3466941/147‑1 (MQ=25)
                                                                                                                           ccGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTc                            >  2:4456712/1‑150 (MQ=255)
                                                                                                                           ccGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTc                            >  2:6670390/1‑150 (MQ=255)
                                                                                                                             gTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGt                          <  1:8571664/150‑1 (MQ=255)
                                                                                                                             gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGt                          >  1:6202521/1‑150 (MQ=255)
                                                                                                                              tttGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTc                         >  2:4653234/1‑150 (MQ=255)
                                                                                                                              tttGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTc                         >  1:7172025/1‑150 (MQ=255)
                                                                                                                              tttGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTc                         >  1:2573358/1‑150 (MQ=255)
                                                                                                                                tGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCaa                       <  1:1362377/150‑1 (MQ=255)
                                                                                                                                 gTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCaaa                      >  1:2416364/1‑150 (MQ=255)
                                                                                                                                  ttggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCCACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAAt                     <  1:4804802/148‑1 (MQ=255)
                                                                                                                                     gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCAt                  >  1:919457/1‑150 (MQ=255)
                                                                                                                                          aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgcgc             >  2:6079901/1‑150 (MQ=255)
                                                                                                                                             aGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCttt          <  1:4086057/150‑1 (MQ=255)
                                                                                                                                               aCGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCttttt        <  2:2573815/150‑1 (MQ=255)
                                                                                                                                                cGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                                    <  1:1032225/89‑1 (MQ=255)
                                                                                                                                                cGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                                    >  2:1032225/1‑89 (MQ=255)
                                                                                                                                                 gCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCt      >  2:2389252/1‑150 (MQ=255)
                                                                                                                                                 gCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCt      <  2:2246229/150‑1 (MQ=255)
                                                                                                                                                 gCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTATTTCt      >  1:2517969/1‑150 (MQ=255)
                                                                                                                                                   acaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCttt    <  1:1412658/150‑1 (MQ=255)
                                                                                                                                                    caGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTa   <  2:6772944/150‑1 (MQ=255)
                                                                                                                                                     aGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAc  >  2:7475239/1‑150 (MQ=255)
                                                                                                                                                     aGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAc  >  2:6579498/1‑150 (MQ=255)
                                                                                                                                                     aGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAc  >  2:3363601/1‑150 (MQ=255)
                                                                                                                                                     aGCGTCGCATAAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAc  >  2:5006984/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAC  >  NC_000913/3392179‑3392477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: