Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,390 C→T 5.5% intergenic (+196/+33) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,3900CT 5.5% 1448.3 / 42.7 437intergenic (+196/+33)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base C (202/210);  new base T (13/11);  total (215/222)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

TTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAG  >  NC_000913/3423241‑3423539
                                                                                                                                                      |                                                                                                                                                     
tttCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTAAGCAAAGTTCAAATACCCTCCCGGCGGATTTTTCCAACTCAGGAGAGTGTTCACCGACAAACAACAGATTAAACAAAATGcc                                                                                                                                                       >  1:1750514/1‑150 (MQ=14)
     cTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGTCGCAACCTTAAAATCAGCTCCCGGCGGATTTGTCCTACACGGGAGAGTGTTCACCGACAAACAACAGAAAAAAAAAAAGGCCCAGTc                                                                                                                                                  >  1:350563/1‑150 (MQ=14)
     cTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGGCGCAACCTTCAAAAACCCACCCCGCCGCTTTGTACGACACGGGAGAGTGTTCACCGCCAAACACACGATTAAACAAAACGCCCCGTc                                                                                                                                                  >  1:1592891/1‑150 (MQ=9)
         atttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCCAGTCTTc                                                                                                                                               >  2:9229497/1‑150 (MQ=255)
          tttgatGTCTGGCTGTTTAGGGCGGGGGTCCTGCCCGCCACCCCCCGGGCCGTTGCTTCGCAACGTTAAAATCCGCTCCCGGCGGTTTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCg                                                                                                                                             <  1:995614/150‑1 (MQ=14)
          tttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCGACCTTAAAATAAGCACCCCCCGGGGTTGTGCGACACGGGGGAGTGTTGACCCACAAAAAACAAATAAAAAAAAAAGCCCAGTAttaag                                                                                                                                             >  1:3215134/1‑147 (MQ=9)
            tgttGTCTGGCTGTTTTGGGGGGGGGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGTATTTGTCCTACTCGGGTGTGTGTTCACCGACAAACAACAGATAAAAAAAAAGGCCCAGTCTTCCGAc                                                                                                                                           <  1:854294/147‑1 (MQ=14)
            tgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                                                                                                                           <  2:1251055/150‑1 (MQ=39)
              atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTg                                                                                                                                         >  2:8813823/1‑150 (MQ=39)
                gTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACATATAAAACAAAAGGCCCAGTCTTCCGACTGAg                                                                                                                                       <  1:1392568/150‑1 (MQ=38)
                 tcgggCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                                                                                                                      <  2:6173355/147‑1 (MQ=37)
                 tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGGTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                                                                                                                      >  2:1743967/1‑150 (MQ=38)
                   tGGCAGTTTATGGCGGGCGTCATGCCCGCCACCCTCCGGGCCGATGCTTCGCAACGTTAAAATCCGCTCACGGCGGAGTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCATCCGACTGAGCCt                                                                                                                                    >  2:641161/1‑150 (MQ=11)
                    ggCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCCCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtt                                                                                                                                   <  2:842858/150‑1 (MQ=37)
                    ggCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtt                                                                                                                                   >  2:6919723/1‑150 (MQ=37)
                     gCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCTCAGTCGGAAGACTGGGCCttt                                                                                                                                  <  1:4799381/150‑1 (MQ=11)
                      ctgTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCTTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtttt                                                                                                                                 <  1:5209295/148‑1 (MQ=255)
                        gTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGt                                                                                                                               <  2:5415469/150‑1 (MQ=14)
                         tttAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAAATAAAACAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGtt                                                                                                                              <  1:6710211/150‑1 (MQ=14)
                            agggcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTa                                                                                                                           <  1:7924558/148‑1 (MQ=39)
                            agggcgggcgTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGTTTTGTCCTACTCGGGAGAGGGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTa                                                                                                                           <  1:2767699/148‑1 (MQ=255)
                            aTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGGTTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACGGAGCCGTTTGttttt                                                                                                                           >  2:2459821/1‑149 (MQ=255)
                                 gggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                                      <  1:3330258/150‑1 (MQ=39)
                                  ggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAt                                                                                                                     <  1:8240839/150‑1 (MQ=39)
                                    cgTCCTGCCCGCCACCCTCCGGGCCGTCGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGt                                                                                                                   >  2:3144094/1‑150 (MQ=255)
                                      tCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                                 <  1:2265817/150‑1 (MQ=255)
                                      tCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                                 <  2:7223632/150‑1 (MQ=255)
                                        cTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAACCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAACCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTgg                                                                                                               <  1:4387540/150‑1 (MQ=255)
                                          gCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCa                                                                                                             <  1:203785/150‑1 (MQ=255)
                                           cccGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAg                                                                                                            >  2:1569878/1‑150 (MQ=255)
                                             cGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGtt                                                                                                          <  2:3915999/150‑1 (MQ=255)
                                                cACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTccc                                                                                                       <  2:2401657/150‑1 (MQ=255)
                                                       cGGGCCGTTGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAACCAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACtctc                                                                                                <  1:6261087/150‑1 (MQ=255)
                                                         ggCCGTTGCTTCGCAACGTTCAAAGCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAACCAACAGATAAAACAAAAGGCCCAGTCGTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGc                                                                                              <  1:4011654/150‑1 (MQ=255)
                                                                gcgtCGCAACGTTCAAATCCGCGCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGga                                                                                       <  1:6188515/147‑1 (MQ=255)
                                                                 cTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgag                                                                                      >  1:7483265/1‑150 (MQ=255)
                                                                  ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                                     >  1:6811634/1‑150 (MQ=255)
                                                                  ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                                     >  1:2104687/1‑150 (MQ=255)
                                                                     gCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAATCAACAGATATTTCTAATGGCCCAGTGTTCCGACTGAGGCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGTccc                                                                                  >  2:8309341/1‑150 (MQ=14)
                                                                     gCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                  <  2:5455608/150‑1 (MQ=255)
                                                                       aaCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCca                                                                                >  2:3350605/1‑150 (MQ=255)
                                                                         cGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                              <  2:2668052/150‑1 (MQ=255)
                                                                          gTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                             >  2:7055170/1‑150 (MQ=255)
                                                                           ttCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                                            >  2:7576357/1‑150 (MQ=255)
                                                                            tCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                           <  1:2493271/150‑1 (MQ=255)
                                                                               aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCa                                                                        >  2:4731060/1‑150 (MQ=255)
                                                                               aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCa                                                                        >  2:8233814/1‑150 (MQ=255)
                                                                                 tCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                      <  2:58404/150‑1 (MQ=255)
                                                                                 tCCGCTCCCGGCGGATTTGTCCTACTCGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCg                                                                     >  1:3446222/1‑150 (MQ=255)
                                                                                    gCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgc                                                                   <  1:49665/150‑1 (MQ=255)
                                                                                      tCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                                                 <  2:4995201/150‑1 (MQ=255)
                                                                                        ccGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                               >  2:8858499/1‑150 (MQ=255)
                                                                                         cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAc                                                              >  2:6274713/1‑150 (MQ=255)
                                                                                         cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAc                                                              >  1:1964589/1‑150 (MQ=255)
                                                                                              gATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                         <  1:560331/150‑1 (MQ=255)
                                                                                                  tGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                     <  2:4364340/150‑1 (MQ=255)
                                                                                                    tCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACt                                                   <  1:7781962/150‑1 (MQ=255)
                                                                                                     ccTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                                  <  1:733562/150‑1 (MQ=255)
                                                                                                     ccTACTCGGGAGAGTGTTCACCGACAAAAAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                                  >  1:6366531/1‑150 (MQ=255)
                                                                                                          tCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCCCTTCTGAg                                             >  2:1864384/1‑150 (MQ=255)
                                                                                                            gggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                           >  2:2920642/1‑150 (MQ=255)
                                                                                                             ggAGAGTGTTCACCGACAAACAACAGATAAAACAACAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                          <  2:2750987/150‑1 (MQ=255)
                                                                                                             ggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                          >  1:2625886/1‑150 (MQ=255)
                                                                                                                gagTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGc                                       >  1:4217000/1‑150 (MQ=255)
                                                                                                                  gtgtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACGTCTGAGGTCGGCAt                                     >  1:7682756/1‑150 (MQ=255)
                                                                                                                    gtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg                                   >  2:364622/1‑150 (MQ=39)
                                                                                                                    gtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg                                   >  1:8186017/1‑150 (MQ=39)
                                                                                                                    gtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg                                   <  1:4715456/150‑1 (MQ=39)
                                                                                                                     ttCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                                  <  1:3843068/150‑1 (MQ=39)
                                                                                                                      tCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg                                 >  2:8730758/1‑150 (MQ=39)
                                                                                                                        aCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTc                               >  1:837326/1‑150 (MQ=39)
                                                                                                                          cGACACACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                             <  2:3980562/150‑1 (MQ=255)
                                                                                                                           gACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAgg                            >  2:7916538/1‑150 (MQ=39)
                                                                                                                            ccagacatcaAATAAAACAAAAGGCTCAGTCGGAAGACGGGGCCTTTGGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTCACTTCTGAGTTCGGCAGGGGGGCAGGt                           >  2:4801323/5‑150 (MQ=11)
                                                                                                                            acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                           <  2:3215134/150‑1 (MQ=39)
                                                                                                                            acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                           >  1:6584124/1‑150 (MQ=39)
                                                                                                                            acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                           <  1:3115012/150‑1 (MQ=39)
                                                                                                                            acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                           <  1:8739161/150‑1 (MQ=39)
                                                                                                                              aaacaaCAGATAAAACAAATGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACGCCACACTACCCTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                         <  1:5514869/150‑1 (MQ=255)
                                                                                                                              aaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                         <  1:3721214/150‑1 (MQ=39)
                                                                                                                               aacaacAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                        <  2:2260924/150‑1 (MQ=39)
                                                                                                                                      gATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                 <  2:1409414/150‑1 (MQ=39)
                                                                                                                                      gATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                 <  2:2039762/150‑1 (MQ=39)
                                                                                                                                       aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                >  1:2955675/1‑150 (MQ=39)
                                                                                                                                       aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACagc                >  1:5856743/1‑150 (MQ=255)
                                                                                                                                        tAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg               >  1:1122840/1‑150 (MQ=39)
                                                                                                                                        tAAAACAAAAGGCCCAGTCTTCCGACTGAGCATTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg               <  1:8060856/150‑1 (MQ=255)
                                                                                                                                           aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                                     >  2:5123119/1‑125 (MQ=40)
                                                                                                                                           aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGATCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTa            >  2:3869600/1‑150 (MQ=255)
                                                                                                                                            aCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAc           <  2:8573866/150‑1 (MQ=39)
                                                                                                                                                 aGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccg      >  1:130748/1‑150 (MQ=37)
                                                                                                                                                  ggCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgc     <  2:6609359/150‑1 (MQ=37)
                                                                                                                                                  ggCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgc     >  2:4136013/1‑150 (MQ=37)
                                                                                                                                                     cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                         <  2:1065285/96‑1 (MQ=40)
                                                                                                                                                     cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                         >  1:1065285/1‑96 (MQ=40)
                                                                                                                                                     cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCa   <  1:9229497/150‑1 (MQ=37)
                                                                                                                                                      ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAc                                                    >  2:675549/1‑100 (MQ=40)
                                                                                                                                                      ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg  >  2:7526214/1‑150 (MQ=37)
                                                                                                                                                      ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg  >  2:5200041/1‑150 (MQ=37)
                                                                                                                                                      ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg  <  1:8895129/150‑1 (MQ=37)
                                                                                                                                                      |                                                                                                                                                     
TTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAG  >  NC_000913/3423241‑3423539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: