Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 6.2% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 6.2% 990.7 / 3.4 374intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (131/210);  new base G (16/17);  total (147/227)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAT  >  NC_000913/3504706‑3505003
                                                                                                                                                     |                                                                                                                                                    
   aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                   <  2:1178804/150‑1 (MQ=255)
    aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                  <  1:3491770/150‑1 (MQ=255)
    aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                  <  2:4498279/150‑1 (MQ=255)
     aCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcccc                                                                                                                                                 <  2:3798361/150‑1 (MQ=255)
          tGCTCGTCTCCGTACTCTTCTGCCGCGCCAATCGCATCACATTAACTATTGATAATAAAAGACATTAACCGTAGGCAGGATAAGATGCGCCATCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                            <  2:8617731/150‑1 (MQ=255)
          tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                            <  2:3814001/150‑1 (MQ=255)
              cGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCCCCCGaa                                                                                                                                        >  2:1882206/1‑150 (MQ=255)
               gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAc                                                                                                                                       >  1:5785595/1‑150 (MQ=255)
                tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATGTTACATCCCGTACGTTCCCCACACCCTaaac                                                                                                                                      >  2:3601303/1‑148 (MQ=255)
                  tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAccca                                                                                                                                    >  1:2940684/1‑149 (MQ=255)
                    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                                  <  1:3203616/150‑1 (MQ=255)
                    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCcact                                                                                                                                  >  2:7800597/1‑150 (MQ=255)
                     gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCCAACCCgctc                                                                                                                                 >  2:5737283/1‑150 (MQ=255)
                      aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                <  1:4133177/150‑1 (MQ=255)
                           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                           <  2:8971/150‑1 (MQ=255)
                            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                          <  1:1513830/150‑1 (MQ=255)
                            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                          <  1:570557/150‑1 (MQ=255)
                            tctGCCGCGCCAATCGCATCACATTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                          <  1:7124120/150‑1 (MQ=255)
                               gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTCGGCCGGAGAAGATGCGCCAGCCGCGCATCCGGCGATGCTGGCGCGTGGAATTTGACAGCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCACAgggg                                                                                                                       >  2:8373897/1‑150 (MQ=255)
                               gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                       <  2:5829182/150‑1 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                      <  2:2657913/150‑1 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                      <  2:7375862/150‑1 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCACTCCCCAAACGGGc                                                                                                                      >  1:4244174/1‑150 (MQ=39)
                                  gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                    <  1:2205407/150‑1 (MQ=255)
                                  gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                    <  1:2589477/150‑1 (MQ=255)
                                       cagcGCATCACCTTAACGATTGCGCATAAACGACCTGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCACTCCCCACCTGGGCGAGGGGa                                                                                                               <  2:7447572/147‑1 (MQ=37)
                                         tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                             <  2:5217956/150‑1 (MQ=255)
                                             aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTc                                                                                                         >  2:678330/1‑150 (MQ=255)
                                             aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACACCCCCCAGGCGCGAGGGGAcccgtc                                                                                                         >  1:6637901/1‑146 (MQ=38)
                                             aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTc                                                                                                         >  2:7748336/1‑150 (MQ=255)
                                                 ccTTAACTCTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                     <  2:1044595/150‑1 (MQ=255)
                                                  cTTACCTCTTGATAATAACCGACAGGACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                    <  2:2136001/150‑1 (MQ=38)
                                                  cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTACCCCACACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGATCAGGAc                                                                                                    >  1:4876636/1‑150 (MQ=39)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCACTCCCCAAAGGGGCGAGGGGCCCGTTCAGTACg                                                                                                   >  2:6745471/1‑150 (MQ=255)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                   >  1:6866318/1‑150 (MQ=255)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCACTCCCCAAAGGGGCGAGGGGGCCCGTCAGTAcc                                                                                                   >  2:7942740/1‑149 (MQ=38)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCAACACCCCCAACGGGCGCGGGGGCCGTTCACTaag                                                                                                   >  1:2481871/1‑148 (MQ=37)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                   <  1:1029677/150‑1 (MQ=255)
                                                     aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCCAAGGGGCGCGGGGACCGGTCACTACGGa                                                                                                 >  2:2260331/1‑150 (MQ=39)
                                                        tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                              <  2:3037684/150‑1 (MQ=255)
                                                         aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACACCCCAAACGGGCGAGGGGACCGGTCAGTACGGAAatt                                                                                             >  1:7665756/1‑150 (MQ=39)
                                                         aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCCGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAttt                                                                                             >  2:6078505/1‑150 (MQ=255)
                                                          ttGATAATAAAAGACATTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                            <  2:6002119/150‑1 (MQ=255)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACACCCCAAACGGGCGAGGGGGCCCGTCAGTACCGAAATTa                                                                                            >  1:3674750/1‑150 (MQ=37)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                            <  1:3293606/150‑1 (MQ=255)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                            <  1:5357723/150‑1 (MQ=255)
                                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                           >  2:4945625/1‑150 (MQ=255)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCCACCCCACAACCCCAAGGGGCGGCGGGAACCGTCCGTACGGGATTTAGc                                                                                          >  1:405407/1‑150 (MQ=25)
                                                             ataataAAAGACATTAACTGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                         <  1:618740/150‑1 (MQ=255)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                         <  2:7235074/150‑1 (MQ=255)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCAAAACCAAACCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCt                                                                                         >  2:616473/1‑150 (MQ=38)
                                                              taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                        <  1:8816305/150‑1 (MQ=255)
                                                                ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                      <  1:224969/150‑1 (MQ=255)
                                                                     acaCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTCGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTCCGGAATTTAGCTTGACGGTg                                                                                 <  2:3370754/148‑1 (MQ=255)
                                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTAt                                                                             <  1:438438/150‑1 (MQ=255)
                                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGGTCCCCCCCCCCCAACCCCCACCCCCAAAGGGCCCGGGGGCCCGGAAGTACGGAAATTATCTTGACGGTGGTaa                                                                             >  1:6509730/1‑149 (MQ=19)
                                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                            <  1:92389/150‑1 (MQ=255)
                                                                            aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                          <  1:5581385/150‑1 (MQ=255)
                                                                            aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                          <  2:4374916/150‑1 (MQ=255)
                                                                               cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                       <  2:4277778/150‑1 (MQ=255)
                                                                                 tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                     <  1:1213182/150‑1 (MQ=255)
                                                                                       ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                               <  1:8093726/150‑1 (MQ=255)
                                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATCCAGCATTAAATACt                                                              >  2:9256524/1‑150 (MQ=255)
                                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                              <  1:3543825/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                            <  2:814311/150‑1 (MQ=255)
                                                                                              aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAt                                                        <  1:7750272/150‑1 (MQ=255)
                                                                                               tGCGCCAGCATCGCATCCGGCGATGCGGGCGCGTAGTATTTTACCTCCCGGACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGTAATTTAGCTTGACGGTGCTTTCCAGCATTAAATACTGGCAATa                                                       <  2:5561600/150‑1 (MQ=38)
                                                                                                 cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                     <  2:4189529/150‑1 (MQ=255)
                                                                                                 cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                     <  1:5059674/150‑1 (MQ=255)
                                                                                                    cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                  <  2:4601975/150‑1 (MQ=255)
                                                                                                       catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                               <  1:6486305/150‑1 (MQ=255)
                                                                                                       catcgcatcCGGCGATGCTGGAGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGGGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                               >  2:8568270/1‑150 (MQ=255)
                                                                                                            catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                                          <  1:5198196/150‑1 (MQ=255)
                                                                                                                 ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCACAATCCgc                                     >  2:7001817/1‑150 (MQ=255)
                                                                                                                 ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                     <  1:3939639/150‑1 (MQ=255)
                                                                                                                  gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                    <  2:1613130/150‑1 (MQ=255)
                                                                                                                  gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCAACCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAATTTAGCTTGAAGGTGCAATACAGCATTAAATACTGGCAATACACAAACGCAAAATACgca                                    >  2:4968311/1‑150 (MQ=37)
                                                                                                                         tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCGCAACCCAAACGGGCCTGGGGACCGTTCAGGACGGAATTTATCATGTCGGTGCTAAACAGCCATAAAAAAAGGCAATACTCACACCCAAAAAACCCAGCAcccg                             >  2:3269986/1‑148 (MQ=255)
                                                                                                                          ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                            >  2:1372191/1‑150 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                           <  2:6485727/150‑1 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                           <  1:8725873/150‑1 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCGCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATACAGCATTAAATACTGGCAATACACTCAAGCAAAATACGCAGCACCGtct                           >  2:1465886/1‑150 (MQ=38)
                                                                                                                            cgcgTTGAATTTTACACCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCACGGGGCGAGGGGACCGTTCAGTACGGAATTGAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAACTCCGCAGCACCGcctc                          <  1:184548/150‑1 (MQ=39)
                                                                                                                             gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTctct                         <  2:297501/150‑1 (MQ=255)
                                                                                                                              cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                        <  1:7446370/150‑1 (MQ=255)
                                                                                                                               gTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGTGGGGACCGTTGAGTTCGGTATTTAGGTTTTCGGTGGTATGCAGCATTAAATTGTGGCAATTCTATGACGCAAAATGCGCAGCACCGTATCTTc                       >  2:7637754/1‑150 (MQ=25)
                                                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                      <  1:7854884/150‑1 (MQ=255)
                                                                                                                                 tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCAATAAATACTGGCAATACTCTAACGCAAAATCCGCAGCACCGtctcataac                     >  2:5363499/1‑145 (MQ=255)
                                                                                                                                 tGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                     <  1:6710727/150‑1 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGTTCCCCGCACCCTAAACCGATCCCCAAATGGGGGAGGGGAGCGGTCAGTCCGGAATTTAGCTTGTCCGGGGTATCCAGCATTAAATACTGGCCATACACGAGCGCCAAATCCGCCGCCCCGTCTCGGCACCGcg                 >  2:2025700/1‑149 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGTTCCCCACACCCTAACCCTCTACCCAAAGGGGCAAGGGGACCGTTCAGTACGGAATTTAGCTTGACCGTGCTATCCAGCAATAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCCCt                 >  2:2458475/1‑150 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGTTCCCCACACCCTAACCCACAAACCAAAGGGGCGAGGGGACCGTTCAGTTCGGAATTTAGCTTTACGGGGCGATCCAGCATTTAATACAGGCAATACACTCAAGCCAAATACGCAGCAACCTCACTTAAACGcg                 >  2:4790101/1‑149 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                 <  1:7987878/150‑1 (MQ=255)
                                                                                                                                      tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                <  1:5850843/150‑1 (MQ=255)
                                                                                                                                            tCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc          <  2:5620518/150‑1 (MQ=255)
                                                                                                                                             cccGTACGTTGCCCGCACCCTAAACCACTACCCAAAGGGGCGAGGGGGCCGTTCAGTACGGAATTTAGCTTTACGGTGCTATACAGCAATAAAAAAAGGCAATACTCTCACCCAAAATACCCACCACCCTCTCGTCACCCCAGCGATTCg         >  2:2798910/1‑150 (MQ=255)
                                                                                                                                              ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg        <  2:3726513/150‑1 (MQ=255)
                                                                                                                                              ccGTACGTTCCCCTCACCCAAACCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACAGGCCATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCGGCGATTCgg        >  2:9186236/1‑150 (MQ=39)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt  <  1:8189579/150‑1 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt  >  2:6556816/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAT  >  NC_000913/3504706‑3505003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: