Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,873 T→A 14.1% intergenic (+90/+62) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8730TA14.1% 965.1 / 27.0 374intergenic (+90/+62)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (96/205);  new base A (56/2);  total (167/207)
Fisher's exact test for biased strand distribution p-value = 7.89e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

TCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCT  >  NC_000913/3504724‑3505022
                                                                                                                                                     |                                                                                                                                                     
   gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCGCACCCAAACCCcctc                                                                                                                                                    >  1:6397877/1‑150 (MQ=255)
       tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTGGGCGTTGACATCCCGGACGTTGCCCTCACCCTACCCCGCGccccc                                                                                                                                                >  2:6424552/1‑149 (MQ=37)
         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACACCCCcaa                                                                                                                                              >  1:791204/1‑150 (MQ=39)
         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                              <  2:8971/150‑1 (MQ=255)
         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGccccttt                                                                                                                                              <  2:5528849/150‑4 (MQ=255)
          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                             <  1:570557/150‑1 (MQ=255)
          tctGCCGCGCCAATCGCATCACATTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                             <  1:7124120/150‑1 (MQ=255)
              ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                                         <  2:7375862/150‑1 (MQ=255)
              ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCTAACCCGCTCCCCAAAGGGGc                                                                                                                                         >  1:5850701/1‑150 (MQ=255)
                gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                       <  1:2589424/150‑1 (MQ=255)
                 cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                      <  2:4163677/150‑1 (MQ=255)
                 cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACACCCCAAACGGGCGAg                                                                                                                                      >  2:253704/1‑150 (MQ=39)
                   ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                    <  2:5841407/150‑1 (MQ=255)
                   ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCCAACCCCCACCCCACAgggggggggg                                                                                                                                    >  2:3535428/1‑150 (MQ=38)
                    cAATCGCATCACCTTAACTATTGATAATAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggt                                                                                                                                   <  1:9109180/150‑2 (MQ=38)
                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                                  <  2:4603711/150‑1 (MQ=255)
                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGAggggc                                                                                                                                  >  2:6552292/1‑149 (MQ=255)
                          cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                             <  1:6692795/150‑1 (MQ=255)
                               ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                        <  2:1407096/150‑1 (MQ=255)
                                cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                       <  1:3089024/150‑1 (MQ=255)
                                cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTACCCCACACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGATCAGGAc                                                                                                                       >  1:4876636/1‑150 (MQ=39)
                                cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTACCCCCCCCCAAAACCACAACCCCAACGGGCCGGGGGTCCGTTccctac                                                                                                                       >  1:8012551/1‑145 (MQ=25)
                                cTTAACTATTGATAATAAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCCCCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTAc                                                                                                                       <  1:4468756/150‑1 (MQ=255)
                                 ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                      <  1:137088/150‑1 (MQ=255)
                                 ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCCCACCCCCAAGGGGCGAGGGGGCCGGTCAGTACg                                                                                                                      >  1:2698551/1‑150 (MQ=38)
                                 ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCACTCCCCAAAGGGGCGAGGGGGCCCGTCAGTAcc                                                                                                                      >  2:7942740/1‑149 (MQ=38)
                                 ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                      <  1:1029677/150‑1 (MQ=255)
                                     cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAAt                                                                                                                  >  2:7654668/1‑150 (MQ=255)
                                      tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                                 <  2:3037684/150‑1 (MQ=255)
                                       aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAttt                                                                                                                >  2:6078553/1‑150 (MQ=255)
                                       aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACACCCCAAACGGGCGAGGGGACCGGTCAGTACGGAAatt                                                                                                                >  1:7665756/1‑150 (MQ=39)
                                         tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCTCCCCCAAGGGGCGAGGGGACCGTGCAGTACGGAATTTAg                                                                                                              >  2:4945660/1‑150 (MQ=255)
                                           ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                            <  2:7235074/150‑1 (MQ=255)
                                            taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                                           <  1:8816305/150‑1 (MQ=255)
                                              ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                         <  1:224969/150‑1 (MQ=255)
                                                 aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAGGGGCGCTGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                                      >  2:5744683/1‑150 (MQ=39)
                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTAt                                                                                                >  2:9237313/1‑150 (MQ=255)
                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCGCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCATGTCCGTGCTAt                                                                                                >  2:1168717/1‑150 (MQ=38)
                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCAACCAAAACCACACCCCCAAAGGGCCGGGGGGCCGGGAAGTACCGAAAATAGCTTGGAGGTGCTAt                                                                                                >  1:2198164/1‑150 (MQ=19)
                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGGTCCCCCCCCCCCAACCCCCACCCCCAAAGGGCCCGGGGGCCCGGAAGTACGGAAATTATCTTGACGGTGGTaa                                                                                                >  1:6509730/1‑149 (MQ=19)
                                                        ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCACAACCCAAAGGGGCGAGGGGCCCGTTCAGGAAGGAAATTATCTTGACGGTGGTAta                                                                                               >  2:291060/1‑149 (MQ=37)
                                                         tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGCGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                                              >  2:4524700/1‑150 (MQ=255)
                                                                 ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGCCCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaa                                                                                      >  2:5614085/1‑150 (MQ=255)
                                                                  gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                     <  1:8109596/150‑1 (MQ=255)
                                                                  gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                     <  2:4269747/150‑1 (MQ=255)
                                                                   ccGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAAATTATCTTGGCGGTGCTATACACCATTAAAt                                                                                    >  2:216095/1‑150 (MQ=38)
                                                                       attcaacgcgcCAGCATCGCCGGATGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGGGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAATACTg                                                                                >  2:2297858/8‑150 (MQ=38)
                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:6802715/150‑1 (MQ=255)
                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:380884/150‑1 (MQ=255)
                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  1:5377702/150‑1 (MQ=255)
                                                                        tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                               <  2:1478254/150‑1 (MQ=255)
                                                                        tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                               <  2:814311/150‑1 (MQ=255)
                                                                           gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                            <  1:3575092/150‑1 (MQ=255)
                                                                                gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCGAAGGGGCGCGGGGACCGGTCAGTACGGAAGTTAGCTTGACGGTGCTATCCCGCATTAAATACTGGCAATActc                                                                       >  2:6002413/1‑150 (MQ=39)
                                                                                  cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                                     <  1:4261731/150‑1 (MQ=255)
                                                                                   aGCGTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                                    <  1:3251085/150‑1 (MQ=255)
                                                                                   aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAATGGCAATACTCTCa                                                                    >  2:5335309/1‑150 (MQ=255)
                                                                                    gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                   <  1:4099726/150‑1 (MQ=255)
                                                                                          catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                                                             <  1:5198196/150‑1 (MQ=255)
                                                                                              cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTCGCAATACTCTCACGCAAAATCCg                                                         <  2:2720373/150‑1 (MQ=255)
                                                                                               ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCACAATCCgc                                                        >  2:7001817/1‑150 (MQ=255)
                                                                                               ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                        <  1:6826255/150‑1 (MQ=255)
                                                                                                   aTGCTGGCGCGTTGAATTTTACATCCCGTACTTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGTGGACCGTTCAGTACGGAATTTAGCTTTACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagca                                                    <  2:8596850/150‑1 (MQ=255)
                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCAACCTAAACCTCTAGCCAAAGGGGGGGGGGGGGGGTTGATTACGGTATTTATGTTTTCGGTGGTATTCAGGATTATATTGTTGGAATTATGTGACGGAAAATACGGAGCACCGt                                                >  2:1931003/1‑150 (MQ=255)
                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCGCAACCCAAACGGGCCTGGGGACCGTTCAGGACGGAATTTATCATGTCGGTGCTAAACAGCCATAAAAAAAGGCAATACTCACACCCAAAAAACCCAGCAcccg                                                >  2:3269986/1‑148 (MQ=255)
                                                                                                          cgcgTTGAATTTTACACCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCACGGGGCGAGGGGACCGTTCAGTACGGAATTGAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAACTCCGCAGCACCGcctc                                             <  1:184548/150‑1 (MQ=39)
                                                                                                           gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTctct                                            <  2:297501/150‑1 (MQ=255)
                                                                                                           gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTctct                                            <  2:4355097/150‑1 (MQ=255)
                                                                                                            cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                                           <  1:7446370/150‑1 (MQ=255)
                                                                                                              atGAATTTTACAACCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                                         <  1:2525119/149‑1 (MQ=255)
                                                                                                               tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                        >  2:7754232/1‑150 (MQ=255)
                                                                                                                 aaTTTTACATCCCGTACGTTCCCCGCCCCCAAACCCGCTCCCCAAAGGGGCGCGGGGCCCGTTCAGTACCGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACACACGCCAAAACCCCAGCACCGTCACTTCACCg                                      >  2:8483720/1‑150 (MQ=255)
                                                                                                                   ttttACATCCCGTACGTTCCCCACACCCTAACCCACAAACCAAAGGGGCGAGGGGACCGTTCAGTTCGGAATTTAGCTTTACGGGGCGATCCAGCATTTAATACAGGCAATACACTCAAGCCAAATACGCAGCAACCTCACTTAAACGcg                                    >  2:4790101/1‑149 (MQ=255)
                                                                                                                   ttttACATCCCGTACGTTCCCCACACCCCAAACCAAACCCCAAAAGGGCGAGGGGACCCGTCAGTAAGGAAATTAGCATTACGGTGCTATACAGCAATAAAAAAAGGCAAAAAAATCACCCAAAAAACCCACCACCCACTCTTCAcccca                                    >  2:2025596/1‑147 (MQ=255)
                                                                                                                    tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                                   <  2:6637901/150‑1 (MQ=255)
                                                                                                                            ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                           <  1:3319286/150‑1 (MQ=255)
                                                                                                                            ccGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGCGGGGACCGGTCAGTACGGAATTTAGCTTTACGGTGCTAACCCGCAATAAATACAGGCAATACTCTCACGCAAAATCCGCAGCACCCTCACTTCACCCCTGCGATTCgg                           >  2:7505035/1‑150 (MQ=255)
                                                                                                                             cGTACGTTCCCCACACCCTAAACCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCACACGCAAAATACGCAGCACCGTCTCTTCACCGCAGCGATTCggg                          >  2:3670254/1‑150 (MQ=38)
                                                                                                                                 cggtCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                      <  2:5656705/147‑1 (MQ=255)
                                                                                                                                  ggtCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCATGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt                     <  1:5567087/148‑1 (MQ=255)
                                                                                                                                        cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                >  1:5023737/1‑85 (MQ=255)
                                                                                                                                        cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:5023737/85‑1 (MQ=255)
                                                                                                                                         tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              >  1:2359883/1‑150 (MQ=255)
                                                                                                                                         tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              >  1:1696396/1‑150 (MQ=255)
                                                                                                                                         tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              <  1:3994061/150‑1 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac          >  1:4171564/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac          >  2:2450850/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac          >  2:1009501/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTACACCGCTGCGATTCGGGTTAATGCGAATCGcac          >  1:8305699/1‑150 (MQ=255)
                                                                                                                                               tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa        >  1:8447796/1‑150 (MQ=255)
                                                                                                                                                aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAAt       >  1:7731363/1‑150 (MQ=255)
                                                                                                                                                 aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATg      >  2:338600/1‑150 (MQ=255)
                                                                                                                                                 aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATg      >  2:7847578/1‑150 (MQ=255)
                                                                                                                                                  cccTCGCCCCTTTGGGGAGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGt     >  2:9141954/1‑150 (MQ=255)
                                                                                                                                                   ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGtt    <  2:6748171/150‑1 (MQ=255)
                                                                                                                                                    ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  1:383857/1‑150 (MQ=255)
                                                                                                                                                    ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   <  1:6497007/150‑1 (MQ=255)
                                                                                                                                                     tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  2:5155695/1‑150 (MQ=255)
                                                                                                                                                     tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  2:2809557/1‑150 (MQ=255)
                                                                                                                                                     tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  1:9294990/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCT  >  NC_000913/3504724‑3505022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: