Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,483 G→C 6.9% intergenic (+38/+49) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4830GC 6.9% 1283.0 / 6.7 395intergenic (+38/+49)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base G (132/227);  new base C (33/2);  total (166/229)
Fisher's exact test for biased strand distribution p-value = 1.06e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 461 total aligned reads displayed.

GGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAAC  >  NC_000913/3959335‑3959631
                                                                                                                                                    |                                                                                                                                                    
gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                                     <  1:5548129/150‑1 (MQ=255)
gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACTACCACagg                                                                                                                                                     >  2:3556986/1‑150 (MQ=255)
gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCCCCCTCTCCCACcgg                                                                                                                                                     >  1:4372279/1‑150 (MQ=255)
     aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACcgggagag                                                                                                                                                <  1:7032725/150‑1 (MQ=255)
        ttccgATCTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                             <  1:5245593/146‑1 (MQ=255)
        gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                             <  2:5152231/150‑1 (MQ=255)
         cGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCGCCCCGACCCTCTCCCACcgggtgggggag                                                                                                                                            >  1:2936000/1‑150 (MQ=255)
          gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcg                                                                                                                                           <  2:6557644/150‑4 (MQ=255)
          gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAgggagagggaga                                                                                                                                           <  2:2117465/150‑1 (MQ=255)
             aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                                                        <  2:5356883/150‑1 (MQ=255)
             aTGTGAACGAAGCGAGTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                                                        <  2:8861851/150‑1 (MQ=255)
               gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAaca                                                                                                                                      <  1:546006/150‑1 (MQ=255)
                 gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACt                                                                                                                                    >  2:3858973/1‑150 (MQ=255)
                   aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACAACCACAGGGAGAGGGAGAAAACACTCa                                                                                                                                  >  1:4567093/1‑150 (MQ=255)
                   aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCAAAAACACAGGGGGGGGGCGaaaaaaaaca                                                                                                                                  >  2:8643505/1‑144 (MQ=255)
                    cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                 <  1:7462536/150‑1 (MQ=255)
                    cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                 <  1:8838294/150‑1 (MQ=255)
                      aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCCCCCCGCTCCCACAGGGCGAGGGCGAAAAAACCCAAgg                                                                                                                               >  2:6715472/1‑150 (MQ=255)
                       aGCGATTCGCAGCCATGCGATTGAGCTGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                              <  1:4055182/150‑1 (MQ=255)
                       aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                              <  2:2849586/150‑1 (MQ=255)
                           aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACACAAGGCCAtc                                                                                                                          >  1:6229175/1‑150 (MQ=39)
                           aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCACAACCACAGGGAGAGGGAGAAAACACTCAAGGCCAtc                                                                                                                          >  1:6687180/1‑150 (MQ=255)
                             tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCGCACCCCCGCCCGAGACCCAAGGGGGAGGGGGAAAACACACAATGCCAtctc                                                                                                                        >  2:7951968/1‑150 (MQ=255)
                               gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                      <  2:3048785/150‑1 (MQ=255)
                               gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                      <  2:8482341/150‑1 (MQ=255)
                                   ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCACCTGgag                                                                                                                  >  1:6901684/1‑150 (MQ=255)
                                     aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                                <  1:1957847/150‑1 (MQ=255)
                                       gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                              <  1:8371128/150‑1 (MQ=255)
                                       gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGGGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                              >  1:8998327/1‑150 (MQ=255)
                                        cGATTGAGCACGTAGCTAAGAAACGCCCCGCCCACATCACACACACGAAACCCATTCCTGGTGCGCGTTAACAGCCCGCTGATGCCCTCCCCCCGACCCTCTCCCACACGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                                             <  2:7418284/150‑1 (MQ=255)
                                         gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACACGGGGAGGGAGAAAACACACAAGGCCATCTCCTGGAGAAGGcc                                                                                                            >  2:249264/1‑150 (MQ=39)
                                            tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                         <  1:315109/150‑1 (MQ=255)
                                             gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCCCCCCCCACCCCCACCCACACGGAGCGGGAGAAAAAACACAACGCCCTATCCCGGCGAAAGccaagc                                                                                                        >  1:4184164/1‑146 (MQ=255)
                                                  ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                   <  1:1372462/150‑1 (MQ=255)
                                                        taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACACGGAGATGGAGAAAAAACACAAGGCCGACAACAGGAGAACGCCGTGCGAGTAGTTggg                                                                                             >  2:5664979/1‑148 (MQ=255)
                                                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                            <  1:4822157/150‑1 (MQ=255)
                                                          aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCACCCCCCCGGCGAGGGAGAAAACACACAAGGCCATCTCCTGGAGCAGGCCGTGCTATTAGTTGCGGt                                                                                           >  1:6067048/1‑150 (MQ=255)
                                                          aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACAACCACACGGCGGGGGAGAAAACAAAAAAAGCCATATACAGGCGAAGGCCGTGCGATTATTTTCggg                                                                                           >  2:7086003/1‑149 (MQ=255)
                                                          aGAAACTGCGCGGCTATATGACAGATATGAAACGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCCCCCCGACCCACTCCCCCAGGGCGAGGGAGAAAACACTCAAGGCCGTCTCCGGGAGAAGGCCGGGCGATTAGGTGCggg                                                                                           >  2:6848075/1‑149 (MQ=255)
                                                           gAAACTGCGCGGCTATTTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                          <  2:3391442/150‑1 (MQ=255)
                                                           gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCCACCCGCACCCACAGGGAGAGGGAGAAAACACACAAGGCCATCTCCGGGAGCAGGCCGTGCAATTAGTTGAGGTa                                                                                          >  1:7755945/1‑150 (MQ=37)
                                                                tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGTGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                     <  1:249264/150‑1 (MQ=255)
                                                                tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                     <  2:204170/150‑1 (MQ=255)
                                                                      gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                                               <  1:4981800/150‑1 (MQ=255)
                                                                       cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCACCCCCCCACCCACACCCCCAGGGCGAGGGAGACAACACGCAATGCCATCTCCGGGAGAAGGCCGTGGGATTAGTTGCGGGACAGCAACCTAat                                                                              >  1:637268/1‑150 (MQ=255)
                                                                       cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                              <  2:5228906/150‑1 (MQ=255)
                                                                          tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat                                                                           <  1:3341693/150‑1 (MQ=255)
                                                                           atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                          <  1:3858973/150‑1 (MQ=255)
                                                                           atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAACGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                          <  1:3677152/150‑1 (MQ=255)
                                                                             gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACCGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAAGGCCTTGCAATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                        >  2:1517375/1‑150 (MQ=255)
                                                                                  atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                   >  2:5785880/1‑150 (MQ=255)
                                                                                  atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                   <  1:1695126/150‑1 (MQ=255)
                                                                                  atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCAACCCCACCCACACCCCCCGGGCGAGGGGGAAAAAACACAAGGCCTTCTCCGGGGGAAGGCCGTGCGAGTAGTTTCGGTACAGCACCAAAAGGAGGTGatata                                                                   >  2:7289582/1‑149 (MQ=255)
                                                                                   tatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                                                                  <  2:4322406/150‑1 (MQ=255)
                                                                                       aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCACCCACACGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGACGGCCGTGCGCTTAGTTGCGGGACAGCACCATAATGATGTGCTAACCGCAc                                                              >  2:4499219/1‑150 (MQ=37)
                                                                                       aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCCCCCACACCCCCCCGGCGGGGGAGCAAACACACCACGCCCTATCCGGGAGAACTCCGTGCGATTATTTGCGGTACAGCCACATAATGATGTGATTTCCCAAc                                                              >  2:4121270/1‑150 (MQ=255)
                                                                                         aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACAAAAGGCCTTCTCCTGGTGAAGGCCGTGCTATTAGTTGCGGTACACCACCATAATGATGTGATATCCGAAATg                                                            >  2:8290980/1‑150 (MQ=37)
                                                                                            tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                         <  1:9265725/150‑1 (MQ=255)
                                                                                               tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                      <  1:6870043/150‑1 (MQ=255)
                                                                                                gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc                                                     <  1:3488317/150‑1 (MQ=255)
                                                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAAGGCCTTGCTAATAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg                                                   >  2:9245853/1‑150 (MQ=255)
                                                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg                                                   >  2:6227911/1‑150 (MQ=255)
                                                                                                     tGCGGGTTACGTGCGCGCTGCGGCCCTCACCCCGCCCCTCTCCCACCGGGAGAGGGAGAAACCCCTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                                                <  2:6005373/150‑1 (MQ=255)
                                                                                                     tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                                                <  2:2984465/150‑1 (MQ=255)
                                                                                                       cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                              <  1:8617293/150‑1 (MQ=255)
                                                                                                          gTTAAGTGCGCGCTGATGCCCACACCCCCACCCTCACCAAAAGGGAGAGGGATAAAACAAAAAAGGCCATCACCAGGAGAAGGCCTTGCAAATAGTTGCGGTACAGCACCTAAATTATTTGATATACGAACAGGGGGTGCAGCGGGCCgg                                           >  2:9074841/1‑150 (MQ=255)
                                                                                                          gTTAAGGGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTT‑‑GGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                         <  1:2045175/150‑1 (MQ=255)
                                                                                                           ttAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt                                          <  1:7895029/150‑1 (MQ=255)
                                                                                                             aaGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                        <  2:720220/150‑1 (MQ=255)
                                                                                                              aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                       <  2:9176418/150‑1 (MQ=255)
                                                                                                               gTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGc                                      <  1:8554891/150‑1 (MQ=255)
                                                                                                                 gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                    <  1:2715609/150‑1 (MQ=255)
                                                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  2:8745558/150‑1 (MQ=255)
                                                                                                                       tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                              <  2:4592774/150‑1 (MQ=255)
                                                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                             <  1:143256/150‑1 (MQ=255)
                                                                                                                         aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                            <  2:6900917/150‑1 (MQ=255)
                                                                                                                         aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                            <  2:1853498/150‑1 (MQ=255)
                                                                                                                           gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                          <  1:8130824/150‑1 (MQ=255)
                                                                                                                           gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                          <  2:3949254/150‑1 (MQ=255)
                                                                                                                               tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCGATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACacg                      >  2:4696177/1‑148 (MQ=255)
                                                                                                                                  ccccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAgaga                   <  2:7530371/150‑1 (MQ=255)
                                                                                                                                   cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                  <  2:4816148/150‑1 (MQ=255)
                                                                                                                                    ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                 >  2:1628671/1‑150 (MQ=255)
                                                                                                                                     tctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGAGGGGATATCCGAACTGGGTGTGCCGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                >  1:6538034/4‑150 (MQ=255)
                                                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                <  2:1384366/150‑1 (MQ=255)
                                                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                <  1:3009465/150‑1 (MQ=255)
                                                                                                                                      gACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc               <  2:5547641/150‑1 (MQ=255)
                                                                                                                                      gACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc               <  1:227510/150‑1 (MQ=255)
                                                                                                                                         ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  1:7152289/1‑150 (MQ=255)
                                                                                                                                         ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  1:8273376/1‑150 (MQ=255)
                                                                                                                                         ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGGCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  2:3184135/1‑150 (MQ=255)
                                                                                                                                         ccTCTCCCACAGGGAGAGGGAGAAAACACTAAATGCCTTATCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAAATGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  1:953747/1‑150 (MQ=255)
                                                                                                                                          ctctCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt           >  1:5864691/1‑150 (MQ=255)
                                                                                                                                          ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt           >  2:8087366/1‑150 (MQ=255)
                                                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt          >  1:9118296/1‑150 (MQ=255)
                                                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACACGAGACAACCACTTTATc      >  1:1004004/1‑150 (MQ=255)
                                                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc      >  2:6404412/1‑150 (MQ=255)
                                                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa    >  2:8345823/1‑150 (MQ=255)
                                                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa    >  1:5148939/1‑150 (MQ=255)
                                                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa    >  1:1128932/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAAC  >  NC_000913/3959335‑3959631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: