Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,621 A→G 6.7% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,6210AG 6.7% 781.6 / 33.3 260intergenic (+87/‑109)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (70/164);  new base G (9/8);  total (87/173)
Fisher's exact test for biased strand distribution p-value = 5.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

Only 100 of 266 total aligned reads displayed.

ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAA  >  NC_000913/164473‑164770
                                                                                                                                                    |                                                                                                                                                     
ttttCTGGCCGCACGCCCGGCCAAATACTGCCCCGACGCGCGGGACTAAAATTTTTTCTTATGTTTGGTTTTCTCCCTCCCCGTGTGGTCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                      <  1:8320468/147‑1 (MQ=255)
      ggCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATACGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGgaga                                                                                                                                                <  2:3761441/150‑1 (MQ=38)
       gccgcccgacCCGGAAAATAGTCCCCCGCCGCGACGGACTAAAAATTATTCTTACGGTTGTTTTTCGCCCTCTCCCTGTGGGTGTGGGCGGGGGGTTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                                               <  2:2530238/144‑1 (MQ=255)
       gCCGGACGACCCGCCAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTATGGTTGTTTTTCTCCCCCTCCCTGTGGTAGTGGGCGGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                                               <  2:7265758/150‑1 (MQ=255)
        ccGGACGACCGGTAAAATCCTGCACCGACGCGCCGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                                                              <  1:7704719/150‑1 (MQ=37)
         gggACGACCCGGAAAATACTTCACCTACGCGACGTACGAAAAATTATTGTTATGTTTGTTTTTAACCCTCTCCCTGTGGGATCGGGCGGGGGGCAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                                                             <  2:2951417/149‑1 (MQ=255)
          ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTGGCCCCCCACCCCAACCCACACCCACAACGGCgggggg                                                                                                                                            >  1:8130761/1‑150 (MQ=25)
           gccgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa                                                                                                                                           <  2:2248187/148‑1 (MQ=255)
            ccgacCCGGCAACAACGGCCCCGACGCGCCGGCCGAACAAGTTTTCGTACGGTTGGTTTTCCCCCTCTCCCTGGGGGAGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgggtta                                                                                                                                          <  2:8325345/149‑4 (MQ=255)
            cccacCCGCCAAATACGGCACCGCCGCGACGCACCAAAAAGTTTCCTTATGTTTGTTTTACCCCCCCCCCCGGTGGGTGCGGGCCGGGGGGGGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggtta                                                                                                                                          <  2:1971407/147‑4 (MQ=255)
            acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGAAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggtta                                                                                                                                          <  2:8545718/150‑4 (MQ=255)
              cacCCGCAAAATACCCCCCCGCCGCGCGGGACTAAAATGTATTCGTTCGGTGGGTTTTCCCCGTCTCCCTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg                                                                                                                                        <  1:5214362/149‑6 (MQ=255)
                 cgggCAAATACGCCCCCGACGCGACGGACTAAAAAGTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                                                                                     <  2:3683446/148‑1 (MQ=38)
                  cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCACCCAAAACCACAACCACAAGGGCGAGGGGGCCGATCg                                                                                                                                    >  2:1545603/1‑150 (MQ=25)
                    gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCGCACCCAAACCCAAAACCACCAGGGAGAGGGGGCCGATCGAg                                                                                                                                  >  2:3134708/1‑150 (MQ=25)
                      aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCCACCGAAACCCCACCCGCAAGGGGGAGGGGGCCGATCGAGca                                                                                                                                >  2:5307036/1‑150 (MQ=25)
                           cTGCCCCGACGCGCCGGACGAAAAAGTATTCGTACGGTTGGTTGTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt                                                                                                                           <  2:7718288/150‑1 (MQ=39)
                           cTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGGTCACCCTCACCCGAAACCACTCCCACAAGGGGGAGGGGACCGATCGAGCACACAt                                                                                                                           >  2:6896208/1‑150 (MQ=37)
                              ccccGACGCGACGGACTAAAATGTTTTCGTATGGTTGTTTTTCTCCCTCTCCGGGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                                                                        <  1:3937985/148‑1 (MQ=255)
                              cACCGACGCGACGGACGAAAATGTTTTCGTCAGGTTGGTTTTCCCCCTCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                                                                        <  1:3793706/150‑1 (MQ=255)
                               cccgccgCGCCGTCCGAAAAATTTTTTTTATGGTTGGTTTTCGCCCTCTGCGGGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg                                                                                                                       <  1:9327842/149‑1 (MQ=255)
                                ccGACGCGACGTACTAAAAATTTTTCGTATGGTTGTTTTTCCCCCTCTCCGTGTGGTCGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                                                                      <  1:3087090/150‑1 (MQ=255)
                                   ccgcgacgGACGAAAATGTTTTCTTTTGGTTGTTTTTCTCCCTCTCCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                                                                   <  2:5174296/149‑1 (MQ=38)
                                      cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACAACCAAACCCACTCCCTCAAGGGAGAGGGGACCCATCGAGCCCAAAATTTGAGAGata                                                                                                                >  2:6170599/1‑150 (MQ=37)
                                           gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCCAACCCAAACCCAAAACCACAAGGGAGAGGGGACCCATACCGCACAAAATTTTAGATATAtatta                                                                                                           >  1:6929490/1‑149 (MQ=18)
                                           gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACACCCCAAACCACACACACAAGGGGGGGGGGGCCGCTCGAGCCCCAAAATTTAGAGataaaaaa                                                                                                           >  1:4680921/1‑145 (MQ=16)
                                             cgAAAAAGTTTGCGTATGGTTGGTTTTCTCCCTCTCCGGGTGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                                                                         <  1:5983761/150‑1 (MQ=255)
                                             cgAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                                                                         <  2:5416984/150‑1 (MQ=255)
                                             cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                                                                         <  2:9187416/150‑1 (MQ=255)
                                                 aatgttttCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt                                                                                                     <  1:9183158/144‑1 (MQ=38)
                                                  acgTATGCGTAGGGTGGGTTTTCGCCCGCCCCCTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc                                                                                                    <  2:7507718/148‑1 (MQ=255)
                                                    ttATTCTTATGTTTGTTTTTCACCCTCTCCCTGTGGTTGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                                                                  <  1:903594/149‑1 (MQ=255)
                                                    gTATTCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGAGGGCCGGGTTGAGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                                                                  <  1:533878/150‑1 (MQ=255)
                                                    gTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                                                                  <  1:6661782/150‑1 (MQ=255)
                                                            aaGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                                                                          <  2:5317733/150‑1 (MQ=255)
                                                              ggttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag                                                                                        <  1:6552254/150‑1 (MQ=255)
                                                               gttggttTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga                                                                                       <  2:1129164/150‑1 (MQ=255)
                                                                ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACACCCCCACCCACACCCACCAGGGAGAGGGGGCCGCTCGCGCCCCCAAATTGTGTGATATAATATTCTGTCTTGTGACacgagac                                                                                      >  2:3293458/1‑144 (MQ=12)
                                                                ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCACCCCAAACCACACCCACAACGGGGAGGGGGCCGATAGCGCCCAAAAATTGTGGGTTATAATATTGTGTCTTTTTACAgaagaa                                                                                      >  2:4490972/1‑150 (MQ=12)
                                                                ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCAAACCCACGCCCCCCAGGGGGAGGGGGCCCAGCGAGCCCCAAATTTGTGAGTTAAATTATTGTGTCTTGTAACagaagta                                                                                      >  2:3033827/1‑148 (MQ=16)
                                                                ttggttTTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa                                                                                      <  2:3901739/150‑1 (MQ=255)
                                                                ttggttTTCTCCCTCACCCTGTGGGAGAGGGGGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAGGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa                                                                                      <  1:2979503/150‑1 (MQ=255)
                                                                 tggttTACTCCCTCTCCGTGTGTGCGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                                                                     <  1:2804476/150‑1 (MQ=255)
                                                                       tctccctctcccTGTGGGTGAGGGCGGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaaa                                                                               <  1:1545603/150‑1 (MQ=255)
                                                                       tcgccctctcccgGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaaa                                                                               <  1:977176/147‑1 (MQ=255)
                                                                          ccctccccgtGTGGGCGAGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                                                            <  1:3303700/150‑1 (MQ=255)
                                                                            ctctccgtGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg                                                                          <  1:1840695/150‑1 (MQ=255)
                                                                                 cctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt                                                                     <  2:4534737/150‑1 (MQ=255)
                                                                                    gtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACAAAGGAGAGGGGACCGCTACAGCCCAAAATTTGAGAGATAAAATCATATGTATTGGAACAGAAAAACAGACAAACGGGCGTTTtcg                                                                  >  2:5996244/1‑150 (MQ=255)
                                                                                       ggtgggggCGGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                                                               <  1:3219750/145‑1 (MQ=255)
                                                                                       ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                                                               <  1:1069103/150‑1 (MQ=255)
                                                                                       ggAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                                                               <  2:901485/150‑1 (MQ=255)
                                                                                        gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCAAAACCGCACCCACAAGGGCGAGGGGCCCGATAGGGCCCAAAATTTGAGAGTTATATTCTTCTGTCTTGTTAAAGAAGAAAACTAAAACGGGCtttttagccgg                                                              >  2:1392780/1‑143 (MQ=255)
                                                                                             ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACAACGGAGAGGGGCCCGATAGAGCACAAAATTTTAGGGAAAAATTATTATGTCTTGTTAAAGAAGAACAGAAAAAAGGGCTTTTTCGCCGGAatat                                                         >  2:6098366/1‑150 (MQ=255)
                                                                                                cGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc                                                      <  1:29507/150‑1 (MQ=255)
                                                                                                 ggggTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                                     <  1:8362832/150‑1 (MQ=255)
                                                                                                 ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                                     <  2:694673/150‑1 (MQ=255)
                                                                                                 ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                                     <  2:5835836/150‑1 (MQ=255)
                                                                                                   ggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga                                                   <  2:4028380/150‑1 (MQ=255)
                                                                                                   ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga                                                   <  1:5239193/150‑1 (MQ=255)
                                                                                                        gggCCTCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAATGGAGAGGGGACCGATCGAGCACATATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa                                              <  1:2508510/150‑1 (MQ=255)
                                                                                                        gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa                                              <  2:5025573/150‑1 (MQ=255)
                                                                                                         ggCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACAAGGGAGAGGGGACCCAACCAGCACAAAAAATTAGATAAAAAATAATAAGTCATTTAAAAAAAAAACACAAAAAAGGGCTTTTTCGCCAGAAAAATTCGGAGaaaaa                                             >  2:9345931/1‑150 (MQ=255)
                                                                                                          gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg                                            <  2:806771/150‑1 (MQ=255)
                                                                                                           cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc                                           <  1:3289102/150‑1 (MQ=255)
                                                                                                                gctatggcGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGc                                      <  1:5449830/143‑1 (MQ=255)
                                                                                                                   cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg                                   <  1:4130298/150‑1 (MQ=255)
                                                                                                                        gTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATg                              <  2:4762985/150‑1 (MQ=255)
                                                                                                                         ttCACCCTCACCCTAACCCTCTCCCTCAGGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGa                             <  2:4377614/150‑1 (MQ=255)
                                                                                                                            accctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg                          <  1:3264886/150‑1 (MQ=255)
                                                                                                                              cctcaccctAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg                        >  1:4527423/1‑150 (MQ=255)
                                                                                                                               ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa                       <  1:4862118/150‑1 (MQ=255)
                                                                                                                               ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa                       >  1:3192731/1‑150 (MQ=255)
                                                                                                                               ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAGTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa                       >  1:7512357/1‑150 (MQ=255)
                                                                                                                                 caccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGc                     <  2:7702615/150‑1 (MQ=255)
                                                                                                                                  accctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc                    >  1:5525963/1‑150 (MQ=255)
                                                                                                                                  accctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc                    >  1:610335/1‑150 (MQ=255)
                                                                                                                                   ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCa                   <  2:5082123/150‑1 (MQ=255)
                                                                                                                                    cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa                  <  1:8381583/150‑1 (MQ=255)
                                                                                                                                    cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa                  >  1:6745143/1‑150 (MQ=255)
                                                                                                                                      tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGAGGAGAAAAGGCATGGCCGGGAATGACCGCGAGCCCAtt                >  1:7235578/1‑150 (MQ=255)
                                                                                                                                       tccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTg               >  2:5585989/3‑150 (MQ=255)
                                                                                                                                         cccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa             >  2:355900/1‑150 (MQ=255)
                                                                                                                                         cccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCAACTTATGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCCAATGGa             >  1:4880309/1‑150 (MQ=255)
                                                                                                                                         cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCGTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa             <  1:8919834/150‑1 (MQ=255)
                                                                                                                                         cccTCTACATCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGACTATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa             <  2:8388119/150‑1 (MQ=255)
                                                                                                                                          ccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc            >  2:8715333/1‑150 (MQ=255)
                                                                                                                                          ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc            <  2:8130761/150‑1 (MQ=255)
                                                                                                                                           ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg           >  2:6596451/1‑150 (MQ=255)
                                                                                                                                           ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg           >  1:5795774/1‑150 (MQ=255)
                                                                                                                                            tctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa             <  1:8643849/147‑1 (MQ=255)
                                                                                                                                               cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaaa       <  2:1731029/150‑1 (MQ=255)
                                                                                                                                                ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg      <  1:2029655/150‑1 (MQ=255)
                                                                                                                                                ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg      <  1:6417497/150‑1 (MQ=255)
                                                                                                                                                 cTCACGGGAGAGGGGACCGCTCGAGCACAAATCTTGAGAGATATCTTCTTCTGTCTTGTAACAGCAGAACAGAAAATCGGGCGTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg     <  1:7915820/150‑1 (MQ=255)
                                                                                                                                                 cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg     <  1:2382463/150‑1 (MQ=255)
                                                                                                                                                  tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg    >  1:1682294/1‑150 (MQ=255)
                                                                                                                                                  tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg    >  2:222138/1‑150 (MQ=255)
                                                                                                                                                   cAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGa   <  1:8183221/150‑1 (MQ=255)
                                                                                                                                                    aaGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGaa  >  1:6520008/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAA  >  NC_000913/164473‑164770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: