Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,005,308 A→T 5.6% A149A (GCA→GCT pldA → outer membrane phospholipase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,005,3080AT 5.6% 2129.3 / 13.1 699A149A (GCA→GCTpldAouter membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base A (322/312);  new base T (3/60);  total (325/374)
Fisher's exact test for biased strand distribution p-value = 4.18e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 711 total aligned reads displayed.

GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457
                                                                                                                                                      |                                                                                                                                                     
 ggATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAg                                                                                                                                                      <  1:5260742/150‑1 (MQ=255)
  gATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCTTTTTGCAgg                                                                                                                                                     >  2:7859706/1‑150 (MQ=255)
  gATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAgg                                                                                                                                                     <  1:4942167/150‑1 (MQ=255)
   aTTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGt                                                                                                                                                    <  2:7430464/150‑1 (MQ=255)
    ttttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGtt                                                                                                                                                   <  1:3268392/150‑1 (MQ=255)
     tttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTg                                                                                                                                                  <  1:5047721/150‑1 (MQ=255)
       tAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGa                                                                                                                                                <  1:2124553/150‑1 (MQ=255)
        aGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGAc                                                                                                                                               <  1:7572277/150‑1 (MQ=255)
          gCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGc                                                                                                                                             >  2:2961705/1‑150 (MQ=255)
            ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTg                                                                                                                                           >  1:1701596/1‑150 (MQ=255)
                 cTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGa                                                                                                                                      <  1:5971478/150‑1 (MQ=255)
                 cTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGa                                                                                                                                      <  2:3982959/150‑1 (MQ=255)
                  tCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAt                                                                                                                                     >  1:8201841/1‑150 (MQ=255)
                   cGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                                                                                                                                    <  2:6249642/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  1:7826737/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  2:8389298/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  2:604938/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAAGAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  2:8525922/150‑1 (MQ=255)
                      tgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                                                                                                                                 <  2:3670348/150‑1 (MQ=255)
                      tgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                                                                                                                                 <  2:4076723/150‑1 (MQ=255)
                         tGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGgaga                                                                                                                              <  2:8216296/150‑1 (MQ=255)
                         tGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGgaga                                                                                                                              <  1:5474436/150‑1 (MQ=255)
                          gggTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGAt                                                                                                                             >  1:3148405/1‑150 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           <  2:1270927/150‑1 (MQ=255)
                             tGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATggg                                                                                                                          >  2:3847622/1‑150 (MQ=255)
                             tGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATggg                                                                                                                          <  2:8684800/150‑1 (MQ=255)
                                 tCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGtat                                                                                                                      <  2:8909712/150‑1 (MQ=255)
                                   ttATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    >  2:9317223/1‑150 (MQ=255)
                                   ttATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    >  1:4273713/1‑150 (MQ=255)
                                       aCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCAc                                                                                                                <  1:6148317/150‑1 (MQ=255)
                                       aCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCAc                                                                                                                <  1:4124985/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              <  2:6925877/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              >  1:8677239/1‑150 (MQ=255)
                                          cAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGAc                                                                                                             >  1:2605166/1‑150 (MQ=255)
                                               aTCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTaa                                                                                                        <  2:5351193/150‑1 (MQ=255)
                                                  cTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACgg                                                                                                     >  1:8309120/1‑150 (MQ=255)
                                                   tggtggCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACggg                                                                                                    >  2:3225998/1‑150 (MQ=255)
                                                      tggCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGt                                                                                                 >  1:2263295/1‑150 (MQ=255)
                                                      tggCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGt                                                                                                 >  1:3423365/1‑150 (MQ=255)
                                                       ggCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGtt                                                                                                >  2:3079502/1‑150 (MQ=255)
                                                        gCAAGTGCCAAATCGCGACGTGTCTACACGGTTTGGTCAAACAAACTCCCACCCGAATTGGTCCCCGGTTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTc                                                                                               <  2:427418/150‑1 (MQ=255)
                                                        cgccgcgTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCGGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTc                                                                                               <  2:6605734/144‑1 (MQ=255)
                                                          aaCTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTccg                                                                                             >  1:944947/1‑150 (MQ=255)
                                                            cTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCATTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTccgac                                                                                           <  2:2075369/150‑1 (MQ=255)
                                                             tGTCCAATAGCCAAGTGTCTTCACCTTTTCGTTAAACAAACTACCAACCGAAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGATGGTTGGACGCTGCGCGATGTGGTGATGGGTTATAACCACGACTCTAACGGGCGTTccgacc                                                                                          <  1:1196653/150‑1 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     <  2:5838001/150‑1 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     >  2:6314040/1‑150 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     >  2:5285154/1‑150 (MQ=255)
                                                                     aGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTcc                                                                                  <  2:2321102/150‑1 (MQ=255)
                                                                      gCGAAGTGTCTACACCGTTTCTTTAAACCAACTACCACCCGCATTTGTGCCTGGTTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGTTGTGGTGAGGGGTTACACCCACGACTCTAACGGGCGTTCCGACCCGACCTccc                                                                                 <  1:7946388/150‑1 (MQ=255)
                                                                         aaGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCa                                                                              <  1:1424359/150‑1 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       >  2:7216348/1‑150 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       >  1:8916238/1‑150 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       >  2:5483166/1‑150 (MQ=255)
                                                                                 tCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAc                                                                      >  1:6936969/1‑150 (MQ=255)
                                                                                 tCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAc                                                                      >  1:743428/1‑150 (MQ=255)
                                                                                   aCCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCg                                                                    >  2:6960120/1‑150 (MQ=255)
                                                                                   aCCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCg                                                                    >  2:3389098/1‑150 (MQ=255)
                                                                                    cggTTTCTTTAAACCAACTACGACCCGAAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGCACGCTGCGCGTTGTGGTGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGc                                                                   <  2:7078776/148‑1 (MQ=255)
                                                                                    ccGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGTTGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGc                                                                   <  2:2470221/150‑1 (MQ=255)
                                                                                    ccGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGAATACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGc                                                                   >  2:4450280/1‑150 (MQ=255)
                                                                                       tttCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCtt                                                                <  2:5922066/150‑1 (MQ=255)
                                                                                       tttCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCtt                                                                >  1:724490/1‑150 (MQ=255)
                                                                                            tGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATAc                                                           >  1:902281/1‑150 (MQ=255)
                                                                                            tGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATAc                                                           >  2:2619913/1‑150 (MQ=255)
                                                                                                aCCAACTACGAACCGCATTTGTCCCTCGGTTTTGCCACCGTTTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGc                                                       <  2:6539411/150‑1 (MQ=255)
                                                                                                aCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGc                                                       <  2:1483196/150‑1 (MQ=255)
                                                                                                    aCTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGa                                                   >  2:9220499/1‑150 (MQ=255)
                                                                                                    aCTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGa                                                   >  2:5138473/1‑150 (MQ=255)
                                                                                                     cTACGAACCTCATTTGTTCCTCGGTTTTGCCACCTATTACCTTTTTGATGGTTGGACGCTGCGCGATGTGGATATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACAGCCTTTATACTCGCCTTAt                                                  <  2:4570944/150‑1 (MQ=255)
                                                                                                        cGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGc                                               >  1:848798/1‑150 (MQ=255)
                                                                                                         gAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCa                                              <  1:2989074/150‑1 (MQ=255)
                                                                                                         gAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCa                                              >  2:1603562/1‑150 (MQ=255)
                                                                                                            ccGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaa                                           >  2:6394799/1‑150 (MQ=255)
                                                                                                             cGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaaa                                          >  1:1059165/1‑150 (MQ=255)
                                                                                                                atttGTTCCTGGTTTTTGCCACCTTTTACCTTTTTGCTGTTTGGACGCTGCGCTTTGTGGAGTTGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACg                                       <  1:2391121/148‑1 (MQ=255)
                                                                                                                aaTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACg                                       >  2:7796225/1‑150 (MQ=255)
                                                                                                                   tGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTa                                    <  1:4384091/150‑1 (MQ=255)
                                                                                                                    gTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                   >  1:9223949/1‑150 (MQ=255)
                                                                                                                        cTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActgg                               >  2:8106133/1‑150 (MQ=255)
                                                                                                                          cGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGATATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggct                             <  2:1780326/150‑1 (MQ=255)
                                                                                                                          cGGTTTGGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggct                             <  2:1946833/150‑1 (MQ=255)
                                                                                                                          cGGATTTGCCACCGATTACCCATTTCCAGGTTGGACGCTCCGCGATGTGCACATGGGGAATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCATTATACTCGCCTGATGGCAGAAAACGGTAActggct                             <  1:5738548/150‑1 (MQ=255)
                                                                                                                           ggTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggctg                            <  2:9075819/150‑1 (MQ=255)
                                                                                                                           ggTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggctg                            >  1:8820981/1‑150 (MQ=255)
                                                                                                                           ggTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATAATCGCCTGATGGCAGAAAACGGTAActggctg                            >  1:4460417/1‑150 (MQ=255)
                                                                                                                            gtttttGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggctg                            <  2:1380618/148‑1 (MQ=255)
                                                                                                                               tttGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                         <  1:3617544/150‑1 (MQ=255)
                                                                                                                                    cACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCGGATGGCAGAAAACGGTAACTGGCTGGTAGAAGt                    >  1:4822314/1‑150 (MQ=255)
                                                                                                                                     aCCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTg                   >  2:8789910/1‑150 (MQ=255)
                                                                                                                                           tACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCg             >  2:9004187/1‑150 (MQ=255)
                                                                                                                                              ctttttGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTgg          <  1:3907455/148‑1 (MQ=255)
                                                                                                                                              cGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTgg          <  1:3799397/150‑1 (MQ=255)
                                                                                                                                                ttttGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTa        >  1:1952156/1‑150 (MQ=255)
                                                                                                                                                ttttGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTa        >  1:6564353/1‑150 (MQ=255)
                                                                                                                                                  ttGCTGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtg      <  1:6440762/150‑1 (MQ=255)
                                                                                                                                                   tGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgt     <  1:2822763/150‑1 (MQ=255)
                                                                                                                                                     cAGGTTGGACGCTGCGCGATGTGGTGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtgg   <  2:4955665/150‑1 (MQ=255)
                                                                                                                                                     cAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtgg   >  2:5709742/1‑150 (MQ=255)
                                                                                                                                                      aGGTTGGACGCTGCGCTATGTGTATATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggt  <  2:2840841/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: